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Standard Operating Procedure for Manual Dispensing Tools Copyright© 2021 Eppendorf AG, Germany ErratingoperatingatingN) procedureProcedure procedure for manual dispensing for manual tools dispensing tools Standard Operating Procedure Standard operating procedure for manual dispensing tools Copyright© 2021 Eppendorf AG, Germany. All rights reserved, including graphics and images. No part of this publication may be reproduced without the prior permission of the copyright owner. Eppendorf® and the Eppendorf Brand Design are registered trademarks of Eppendorf AG, Germany. Biomaster®, Combitips® advanced, epT.I.P.S.®, Eppendorf Varitips®, Eppendorf Reference® 2, Eppendorf Research® plus, Eppendorf Xplorer®, Eppendorf Xplorer® plus, Mastertip®, Maxipettor®, Move It®, Multipette® E3, Multipette® E3x, Multipette® M4, Multipette® plus, Multipette® stream, Multipette® Xstream, Repeater® E3, Repeater® E3x, Repeater® M4, Repeater® plus, Repeater® stream, Repeater® Xstream, Varipette®, Varispenser®, Varispenser® plus, Varispenser® 2 and Varispenser® 2x are registered trademarks of Eppendorf AG, Germany. Eppendorf Top Buret ™ is a protected trademark of Eppendorf AG, Germany. Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual. U.S. Design Patents are listed on www.eppendorf.com/ip ASOP P39 020-13/072021 Table of contents Standard Operating Procedure 3 English (EN) Table of contents 1 Operating instructions. 9 1.1 Glossary. 9 1.2 Preface . 14 1.3 Version overview. 15 1.4 Supported Eppendorf dispensers . 16 1.4.1 Mechanical piston stroke pipettes – air-cushion principle . 16 1.4.2 Electronic piston stroke pipettes – air-cushion principle . 16 1.4.3 Mechanical piston stroke pipettes – hybrid system . 16 1.4.4 Mechanical piston stroke pipettes – positive displacement principle. 16 1.4.5 Mechanical multiple-dispensers – positive displacement principle. 16 1.4.6 Electronic multiple-dispensers – positive displacement principle. 16 1.4.7 Mechanical single stroke dispensers – positive displacement principle. 16 1.4.8 Mechanical bottle-top burette – positive displacement principle. 16 2 Cleaning and maintenance information . 17 2.1 Cleaning the pistion stroke pipettes – air-cushion principle . 17 2.1.1 Single-channel pipettes . 17 2.1.2 Multi-channel pipettes with fixed cone spacing (9 mm) . 18 2.1.3 Multi-channel pipettes with fixed cone spacing (4.5 mm) . 18 2.1.4 Multi-channel pipettes with adjustable cone spacing . 18 2.2 Cleaning piston-stroke pipettes – Positive displacement principle. 18 2.3 Cleaning the multi-dispenser – Positive displacement principle . 18 2.4 Cleaning the single stroke dispenser . 19 2.5 Cleaning bottle-top burets. 19 2.6 Decontamination before shipment . 19 3 Test intervals . 20 4 Types of testing . 21 4.1 Visual inspection of all dispensers . 21 4.2 Visual inspection of single stroke dispensers and bottle-top burets . 21 4.3 Testing the tightness of dispensers with air-cushion principle . 21 4.3.1 Dispensing system is tight. 22 4.3.2 Dispensing system is not tight . 22 4.4 Checking the leak tightness of dispensers with positive displacement principle . 22 4.5 Intermediate check – Quick-Check . 22 Table of contents 4 Standard Operating Procedure English (EN) 4.6 Conformity test . 22 5 Prerequisites for gravimetric testing . 23 5.1 Measuring place setup . 23 5.1.1 Analytical balance . 23 5.1.2 Single-channel balance . 23 5.1.3 Multi-channel balance . 23 5.1.4 Weighing vessel. 24 5.1.5 Measuring place . 24 5.2 Test liquid . 24 5.3 Temperature . 24 5.4 Test tips . 25 5.5 Data transfer and data evaluation . 25 5.6 Additional test conditions . 25 6 Performing the calibration . 26 6.1 Preparing the measuring place for calibration . 28 6.1.1 Preparing the dispenser, test liquid and analytical balance . 28 6.1.2 Preparing a reusable 384 box for multi-channel pipettes with 16 channels . 28 6.1.3 Preparing a reusable 384 box for multi-channel pipettes with 24 channels . 29 6.1.4 Preparing the documentation . 29 6.2 Checklists for the preparation of the calibration. 30 6.2.1 A – Test conditions . 30 6.2.2 B – Test liquid . 31 6.2.3 C – Dispenser. 31 6.2.4 D – Analytical balance . 32 6.2.5 E – Calibrating software . 32 6.3 Conducting a series of measurements . 33 6.3.1 Nominal volume. 33 6.3.2 Number of measured values . 33 6.3.3 Number of equipped tip cones - 4-channel and 12-channel lower parts. 33 6.3.4 Number of equipped tip cones - 16-channel and 24-channel lower parts. 33 6.3.5 Testing volume . 34 6.3.6 Presaturating the air cushion . 34 6.3.7 Overview of the calibration procedures. 34 6.3.8 Determining measured values - mechanical single-channel pipettes . 36 6.3.9 Determining measured values - mechanical multi-channel pipettes with 4.5 mm cone distance . 36 6.3.10 Test run I and II . 37 6.3.11 Determining measured values - mechanical multi-channel pipettes with 9 mm cone distance. 38 Table of contents Standard Operating Procedure 5 English (EN) 6.3.12 Determining measured values – mechanical multi-channel pipettes with adjustable cone spacing . 38 6.3.13 Determining measured values - electronic single-channel pipettes . 39 6.3.14 Determining measured values - electronic multi-channel pipettes with 4.5 mm cone distance . 39 6.3.15 Test run I and II . 40 6.3.16 Determining measured values - electronic multi-channel pipettes with 9 mm cone distance. 41 6.3.17 Determining measured values – electronic multi-channel pipettes with adjustable cone spacing. 41 6.3.18 Determining measured values - hybrid systems . 42 6.3.19 Determining measured values - mechanical multiple-dispensers . 42 6.3.20 Determining measured values - electronic multiple-dispensers . 43 6.3.21 Determining measured values - mechanical single stroke dispensers . 43 6.3.22 Determining measured values - mechanical bottle-top burette . 43 7 Evaluating the calibration . 44 7.1 Converting gravimetric measured values to volumes . 45 7.2 Correction factor Z . 46 7.3 Calculating the arithmetic mean volume value. 47 7.4 Calculating the systematic error of measurement . 48 7.4.1.
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