Product Datasheet

MRPL24 Antibody

Catalog No: #34323

Package Size: #34323-1 50ul #34323-2 100ul Orders: [email protected] Support: [email protected]

Description

Product Name MRPL24 Antibody

Host Species Rabbit

Clonality Polyclonal

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific

immunogen.

Applications WB IHC

Species Reactivity Hu Ms

Specificity The antibody detects endogenous levels of total MRPL24 .

Immunogen Type Peptide

Immunogen Description Synthesized peptide derived from internal of human MRPL24.

Target Name MRPL24

Other Names 39S L24; mitochondrial precursor; RM24;

Accession No. Swiss-Prot: Q96A35NCBI ID: 79590

SDS-PAGE MW 32kd

Concentration 1.0mg/ml

Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide

and 50% glycerol.

Storage Store at -20°C

Application Details

Western blotting: 1:500~1:3000

Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from HUVEC cells, using MRPL24 antibody #34323.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MRPL24 antibody #34323.

Background

Mammalian mitochondrial ribosomal are encoded by nuclear and help in protein synthesis within the . Mitochondrial (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by . This gene encodes a 39S subunit protein which is more than twice the size of its E.coli counterpart (EcoL24). Sequence analysis identified two transcript variants that encode the same protein. Kenmochi N., Genomics 77:65-70(2001). Woodfine K., Nature 441:315-321(2006). Ota T., Nat. Genet. 36:40-45(2004).

Note: This product is for in vitro research use only and is not intended for use in humans or animals.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2