CDC5L Antibody Cat. No.: 63-716
CDC5L Antibody
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat- anti-rabbit secondary antibodies were used for the analysis.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
This CDC5L antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 187-213 amino acids from the Central region of human CDC5L.
TESTED APPLICATIONS: Flow, WB
For WB starting dilution is: 1:1000 APPLICATIONS: For FACS starting dilution is: 1:10~50
PREDICTED MOLECULAR 92 kDa WEIGHT:
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This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: CDC5L
Cell division cycle 5-like protein, Cdc5-like protein, Pombe cdc5-related protein, CDC5L, ALTERNATE NAMES: KIAA0432, PCDC5RP
ACCESSION NO.: Q99459
PROTEIN GI NO.: 73619933
GENE ID: 988
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
The protein is a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was BACKGROUND: also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre- mRNA splicing.
1) Grillari,J., et.al., J. Biol. Chem. 284 (42), 29193-29204 (2009) Zhang,N., et.al., EMBO Rep. REFERENCES: 10 (9), 1029-1035 (2009)
ANTIBODIES FOR RESEARCH USE ONLY.
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