Human CD302/CLEC13A Antibody Antigen Affinity-purified Polyclonal Sheep IgG Catalog Number: AF6367

DESCRIPTION Species Reactivity Human

Specificity Detects human CD302/CLEC13A in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross­reactivity with recombinant mouse CD302 is observed. Source Polyclonal Sheep IgG

Purification Antigen Affinity­purified

Immunogen Mouse myeloma cell line NS0­derived human CD302/CLEC13A Ala20­Asn167 Accession # Q8IX05 Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (­SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Western Blot 1 µg/mL See Below Flow Cytometry 2.5 µg/106 cells See Below CyTOF­ready Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

DATA

Western Blot Flow Cytometry Detection of Human CD302/CLEC13A by Western Blot. Western Detection of CD302/CLEC13A in blot shows lysates of HL­60 human acute promyelocytic leukemia cell Human Monocytes by Flow line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti­Human Cytometry. Human peripheral blood CD302/CLEC13A Antigen Affinity­purified Polyclonal Antibody monocytes were stained with Sheep (Catalog # AF6367) followed by HRP­conjugated Anti­Sheep IgG Anti­Human CD302/CLEC13A Affinity Secondary Antibody (Catalog # HAF016). A specific band was detected Purified Polyclonal Antibody for CD302/CLEC13A at approximately 30 kDa (as indicated). This (Catalog # AF6367, filled histogram) experiment was conducted under reducing conditions and using or control antibody (Catalog # 5­001­ Immunoblot Buffer Group 1. A, open histogram), followed by Phycoerythrin­conjugated Anti­Sheep IgG Secondary Antibody (Catalog # F0126).

PREPARATION AND STORAGE Reconstitution Sterile PBS to a final concentration of 0.2 mg/mL. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (­SP) is shipped with polar packs. Upon receipt, store it immediately at ­20 to ­70 °C Stability & Storage Use a manual defrost freezer and avoid repeated freeze­thaw cycles. l 12 months from date of receipt, ­20 to ­70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 6 months, ­20 to ­70 °C under sterile conditions after reconstitution.

BACKGROUND CD302 (also DCL­1 and CLEC13A) is a 31­36 kDa member of the C­type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion. Mature human CD302 is a 210 amino acid (aa) type I transmembrane glycoprotein. It contains a 146 aa extracellular domain (ECD) (aa 23­168) plus a 43 aa cytoplasmic region. The ECD possesses a C­type lectin domain (aa 32­152) that apparently cannot bind carbohydrate. CD302 has also been detected in dendritic cells as the C­terminal portion of a 215 kDa fusion protein that involves DEC205. CD302 contributes aa 23­232 to the fusion protein. There is one potential alternative start site at Met63. Over aa 23­168, human CD302 shares 82% aa identity with mouse CD302.

Rev. 2/6/2018 Page 1 of 1