Supplementary Table S3. GDF-15 induced elevation in the levels of extracellular proteins secreted by human TM cells based on quantitative proteomics analysis

Identified Proteins Accession Experiment Experiment Number 1 2 Protein-glutamine gamma-glutamyltransferase 2 TGM2   Cluster of Serpin B3 SPB3  Pyruvate kinase PKM KPYM   Serpin B3 SPB3  Desmoplakin DESP   Junction plakoglobin PLAK   Beta-actin-like protein 2 ACTBL   Serpin B4 SPB4  Plasminogen activator inhibitor 1 PAI1  Cluster of Myosin-9 MYH9   ELN  Hemicentin-1 HMCN1  Myosin-9 MYH9   Alpha-enolase ENOA   Heat shock protein beta-1 HSPB1   Tubulin beta chain TBB5   Laminin subunit beta-2 LAMB2  core protein CSPG2  Filaggrin FILA   Serine protease 23 PRS23   Serpin B5 SPB5  Cluster of 14-3-3 protein zeta/delta 1433Z  Integrin alpha-5 ITA5   Cluster of Laminin subunit gamma-1 LAMC1   Nidogen-1 NID1   Galectin-7 LEG7   Glyceraldehyde-3-phosphate dehydrogenase G3P   Collagen alpha-2(IV) chain CO4A2   Serum albumin ALBU   Collagen alpha-1(V) chain CO5A1   Filamin-C FLNC  Ubiquitin-60S ribosomal protein L40 RL40   Tubulin alpha-3E chain TBA3E  14-3-3 protein zeta/delta 1433Z 

Downloaded from iovs.arvojournals.org on 10/02/2021 Latent-transforming growth factor beta-binding protein 2 LTBP2  Latent-transforming growth factor beta-binding protein 1 LTBP1  Protein-glutamine gamma-glutamyltransferase E TGM3   Cornulin CRNN  Cilia- and flagella-associated protein 57 CFA57   Cluster of Serine protease HTRA1 HTRA1   Elongation factor 1-alpha 1 EF1A1   Cluster of Tubulin alpha-1B chain TBA1B   Tubulointerstitial nephritis antigen-like TINAL   Alpha-actinin-1 ACTN1   Glia-derived nexin GDN   Nidogen-2 NID2   Laminin subunit alpha-3 LAMA3  14-3-3 protein epsilon 1433E  G-protein coupled receptor 98 GPR98   Glutathione S-transferase P GSTP1   A1 ANXA1   -1 FBLN1   Heat shock-related 70 kDa protein 2 HSP72  Beta-Ala-His dipeptidase CNDP1  Cluster of Actin, cytoplasmic 1 ACTB   Beta-crystallin B2 CRBB2   Elongation factor 2 EF2   Polymerase I and transcript release factor PTRF   Peroxiredoxin-2 PRDX2   Actin, alpha cardiac muscle 1 ACTC   Cluster of Peroxiredoxin-1 PRDX1   Fatty acid synthase FAS  NADH-cytochrome b5 reductase 3 NB5R3  Cluster of Guanine nucleotide-binding protein G(i) subunit GNAI2  alpha-2 Peptidyl-prolyl cis-trans isomerase B PPIB  Endoplasmin ENPL  ANXA2   Collagen alpha-1(XVIII) chain COIA1   Peroxiredoxin-1 PRDX1   ATP synthase subunit beta, mitochondrial ATPB   Thrombospondin type-1 domain-containing protein 4 THSD4   Cluster of Annexin A2 ANXA2  

Downloaded from iovs.arvojournals.org on 10/02/2021 Laminin subunit alpha-5 LAMA5   Integrin alpha-V ITAV   Fanconi anemia group E protein FANCE  Integrin beta-1 ITB1  Biglycan PGS1  Extended -1 ESYT1  Transferrin receptor protein 1 TFR1  Plectin PLEC   ANXA6  Prelamin-A/C LMNA   Peroxiredoxin-6 PRDX6   Aldehyde dehydrogenase, dimeric NADP-preferring AL3A1  Collagen alpha-1(III) chain CO3A1  Thrombospondin-1 TSP1   Collagen alpha-1(I) chain CO1A1  -like protein 5 CALL5   Collagen alpha-1(VIII) chain CO8A1   Periostin POSTN   Laminin subunit beta-1 LAMB1   Metalloproteinase inhibitor 3 TIMP3  

Experiment 1 and 2 were carried out using the TM cells derived from 19 and 71 year-old human donors, respectively.

Downloaded from iovs.arvojournals.org on 10/02/2021