Supplementary Table S3. GDF-15 induced elevation in the levels of extracellular proteins secreted by human TM cells based on quantitative proteomics analysis
Identified Proteins Accession Experiment Experiment Number 1 2 Protein-glutamine gamma-glutamyltransferase 2 TGM2 Cluster of Serpin B3 SPB3 Pyruvate kinase PKM KPYM Serpin B3 SPB3 Desmoplakin DESP Junction plakoglobin PLAK Beta-actin-like protein 2 ACTBL Serpin B4 SPB4 Plasminogen activator inhibitor 1 PAI1 Cluster of Myosin-9 MYH9 Elastin ELN Hemicentin-1 HMCN1 Myosin-9 MYH9 Alpha-enolase ENOA Heat shock protein beta-1 HSPB1 Tubulin beta chain TBB5 Laminin subunit beta-2 LAMB2 Versican core protein CSPG2 Filaggrin FILA Serine protease 23 PRS23 Serpin B5 SPB5 Cluster of 14-3-3 protein zeta/delta 1433Z Integrin alpha-5 ITA5 Cluster of Laminin subunit gamma-1 LAMC1 Nidogen-1 NID1 Galectin-7 LEG7 Glyceraldehyde-3-phosphate dehydrogenase G3P Collagen alpha-2(IV) chain CO4A2 Serum albumin ALBU Collagen alpha-1(V) chain CO5A1 Filamin-C FLNC Ubiquitin-60S ribosomal protein L40 RL40 Tubulin alpha-3E chain TBA3E 14-3-3 protein zeta/delta 1433Z
Downloaded from iovs.arvojournals.org on 10/02/2021 Latent-transforming growth factor beta-binding protein 2 LTBP2 Latent-transforming growth factor beta-binding protein 1 LTBP1 Protein-glutamine gamma-glutamyltransferase E TGM3 Cornulin CRNN Cilia- and flagella-associated protein 57 CFA57 Cluster of Serine protease HTRA1 HTRA1 Elongation factor 1-alpha 1 EF1A1 Cluster of Tubulin alpha-1B chain TBA1B Tubulointerstitial nephritis antigen-like TINAL Alpha-actinin-1 ACTN1 Glia-derived nexin GDN Nidogen-2 NID2 Laminin subunit alpha-3 LAMA3 14-3-3 protein epsilon 1433E G-protein coupled receptor 98 GPR98 Glutathione S-transferase P GSTP1 Annexin A1 ANXA1 Fibulin-1 FBLN1 Heat shock-related 70 kDa protein 2 HSP72 Beta-Ala-His dipeptidase CNDP1 Cluster of Actin, cytoplasmic 1 ACTB Beta-crystallin B2 CRBB2 Elongation factor 2 EF2 Polymerase I and transcript release factor PTRF Peroxiredoxin-2 PRDX2 Actin, alpha cardiac muscle 1 ACTC Cluster of Peroxiredoxin-1 PRDX1 Fatty acid synthase FAS NADH-cytochrome b5 reductase 3 NB5R3 Cluster of Guanine nucleotide-binding protein G(i) subunit GNAI2 alpha-2 Peptidyl-prolyl cis-trans isomerase B PPIB Endoplasmin ENPL Annexin A2 ANXA2 Collagen alpha-1(XVIII) chain COIA1 Peroxiredoxin-1 PRDX1 ATP synthase subunit beta, mitochondrial ATPB Thrombospondin type-1 domain-containing protein 4 THSD4 Cluster of Annexin A2 ANXA2
Downloaded from iovs.arvojournals.org on 10/02/2021 Laminin subunit alpha-5 LAMA5 Integrin alpha-V ITAV Fanconi anemia group E protein FANCE Integrin beta-1 ITB1 Biglycan PGS1 Extended synaptotagmin-1 ESYT1 Transferrin receptor protein 1 TFR1 Plectin PLEC Annexin A6 ANXA6 Prelamin-A/C LMNA Peroxiredoxin-6 PRDX6 Aldehyde dehydrogenase, dimeric NADP-preferring AL3A1 Collagen alpha-1(III) chain CO3A1 Thrombospondin-1 TSP1 Collagen alpha-1(I) chain CO1A1 Calmodulin-like protein 5 CALL5 Collagen alpha-1(VIII) chain CO8A1 Periostin POSTN Laminin subunit beta-1 LAMB1 Metalloproteinase inhibitor 3 TIMP3
Experiment 1 and 2 were carried out using the TM cells derived from 19 and 71 year-old human donors, respectively.
Downloaded from iovs.arvojournals.org on 10/02/2021