DOCSLIB.ORG

Investigation of the Zinc-Mitophagy Signaling in Hypoxic Cells a Dissertation Presented to the Faculty of the College of Arts An

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Investigation of the Zinc-Mitophagy Signaling in Hypoxic Cells a Dissertation Presented to the Faculty of the College of Arts An Investigation of the Zinc-Mitophagy Signaling in Hypoxic Cells A dissertation presented to the faculty of the College of Arts and Sciences of Ohio University In partial fulfillment of the requirements for the degree Doctor of Philosophy Qiping Lu May 2020 © 2020 Qiping Lu. All Rights Reserved. 2 This dissertation titled Investigation of the Zinc-Mitophagy Signaling in Hypoxic Cells by QIPING LU has been approved for the Department of Molecular and Cellular Biology and the College of Arts and Sciences by Yang V. Li Professor of Biomedical Sciences Florenz Plassmann Dean, College of Arts and Sciences 3 ABSTRACT LU, QIPING, Ph.D., May 2020, Molecular and Cellular Biology Investigation of the Zinc-Mitophagy Signaling in Hypoxic Cells Director of Dissertation: Yang V. Li Zinc is one of the most essential trace elements in the body. The concentration of intracellular free zinc is strictly regulated. The abnormal zinc concentration has been implicated in numerous clinical manifestations including ischemic stroke. Zinc homeostasis is achieved by proteins and organelles which sequester zinc or release zinc. Mitochondria are the power plants of the cell, the proper function of mitochondria is crucial for cellular metabolisms and physiological activities, their quality and quantity are regulated by mitophagy through selectively removing damaged mitochondria. Emerging evidence over the past decade has shown that zinc affects mitochondria in response to ischemia. It is progressively clear that zinc-mitochondrial interactions occur in and contribute to ischemic injury. Among the pathological effects of zinc accumulation on mitochondria, zinc induced release and accumulation of ROS draws special interest. ROS is a major cause of mitochondrial damage and initiates mitophagy. In addition, emerging evidence suggests that mitophagy plays critical roles in the pathophysiological process of cerebral ischemia. In this study I investigated the role of zinc and mitochondria in hypoxia-induced mitophagy, screened for and evaluated the cross talks between zinc and mitophagy under hypoxia condition. First, I studied the distribution of intracellular free zinc in multiple types of cell cultures, including HeLa cells, cortical neurons, and pancreatic HIT-T15 cells. The live cell fluorescent imaging with zinc specific probe 4 revealed that mitochondria, ER and the Golgi apparatus are potential zinc storage sites during cellular zinc homeostasis. Among them, mitochondria serve as a major organelle that sequesters zinc. I demonstrated the critical roles of zinc in stroke through my study in in vitro stroke model by showing that zinc chelation alleviates the hypoxia-triggered mitophagy activity, and zinc influx damages mitochondria and induces mitophagy. The interplay between zinc and mitophagy during hypoxia was then investigated by large- scale screening and fine-scale validation. The expression of superoxide dismutase 1 (SOD1) was elevated to defend the oxidative stresses during hypoxia and zinc influx, and during this process, spastic paraplegia 7 (SPG7) was high up-regulated, and possibly participate in the formation of mitochondrial permeability transient pore. In contrast, SOD1 is not responsive to the mitochondrial uncoupler, Carbonyl cyanide-4- (trifluoromethoxy) phenylhydrazone (FCCP), and superoxide dismutase 2 (SOD2) takes the major role in defending FCCP-induced oxidative stress defense. In conclusion, zinc chelation is effective in rescuing cells from stresses induced by stroke/hypoxia by reducing the damages to mitochondria, preserving the mitochondrial integrity and lowering the mitophagy activity. Furthermore, the concept of zinc and mitophagy crosstalk during hypoxia/stroke was demonstrated and investigated. Through the elucidation of zinc-mitophagy crosstalk, more promising interventions can be invented to target the zinc-mitophagy signaling during stroke. 5 DEDICATION I dedicate this work to my father Aijun Lu Who had inspired me to chase a dream in life science . 6 ACKNOWLEDGMENT First and foremost, I would like to express the gratitude to my supervisor, Dr. Yang V Li, for his constant guidance, strong support, valuable teaching, extraordinary patience through my doctorial training at Ohio University. He taught me how to fight through difficulties, solve problems, and maintain optimism. He has not only trained me so that I am capable with research in depth, but also quickly adapted to a past-paved environment with well-trained critical thinking ability. I would also like to express my sincere gratitude to my committee, Dr. Robert Colvin, Dr. Tomo Sugiyama, Dr. Zhihua Hua for maintaining the high standard and giving me valuable suggestions and being very supportive on my work. Moreover, I would like to thank Dr. Sarah Wyatt for her teaching in scientific writing and presentation, especially for her strong support and help in solving the difficulties I have faced. I am grateful to everyone who was or is in the lab: Dr. Christian Stork, Kira Slepchenko, Dr. Zhijun Shen, Dr. Xinge Yu, Yuli Hu, Zihui Wang, Hariprakash Haragopal, and Katherine knies for their assistance, their instructive discussion during my study. In addition, I would like to thank all my friends, Yunyi Feng, Panduan An, Yanrong Qian, Xiaodan Zhao and many more, for their encouragement and support during the past years. Their intelligence and persistence have always inspired me to pursue a better outcome. 7 Last but not least, my deepest appreciation goes to my mother, Linge Liu, my brother and sister-in-law, Qiwei Lu and Yang Li, for their whole-hearted and everlasting support, care and love. 8 TABLE OF CONTENTS Page Abstract ...........................................................................................................................3 Dedication .......................................................................................................................5 Acknowledgment .............................................................................................................6 List of Tables................................................................................................................. 11 List of Figures ............................................................................................................... 12 Abbreviations ................................................................................................................ 14 Introduction ................................................................................................................... 15 1. Zinc ..................................................................................................................... 15 1.1 Zinc Content and its Roles ............................................................................ 15 1.2 Zinc Homeostasis and Trafficking ................................................................ 16 1.3 Fluorescent Detection of Zinc ....................................................................... 17 1.4 Zinc in Cerebral Ischemia............................................................................. 18 2. Hypoxia ............................................................................................................... 19 2.1 Hypoxia Signaling Pathway ......................................................................... 19 2.2 Hypoxia in Stroke ........................................................................................ 20 2.3 Hypoxia Inducing Chemicals........................................................................ 21 3. Mitochondria ....................................................................................................... 22 3.1 Mitochondrial Structure and Function .......................................................... 22 3.2 Mitochondrial Quality Control ..................................................................... 23 3.3 Molecular Mechanisms of Mitophagy .......................................................... 25 3.4 Mitophagy in Hypoxia .................................................................................. 26 4. The Potential Interplay between Zinc and Mitophagy during Hypoxia.................. 27 Specific Aims ................................................................................................................ 29 Specific Aim 1: To Investigate the Intracellular Distribution of Free Zinc ................ 29 Specific Aim 2: To Investigate the Effect of Zinc on Mitophagy during Hypoxia by Modulating the Zinc Levels ..................................................................................... 30 Specific Aim 3: To Investigate How Zinc Interplays with Mitophagy during Hypoxia by Screening and Validating the Candidate Genes involved in the Signaling Pathways ................................................................................................................................ 31 Materials and Methods .................................................................................................. 32 1.Cell Cultures ......................................................................................................... 32 9 2. Zinc Labeling ....................................................................................................... 32 3. Organelle and Zinc Co-labeling............................................................................ 33 4. Fluorescence Imaging .......................................................................................... 34 5. in vitro Treatment to Induce or
Load more

Recommended publications
  • Material Safety Data Sheet Sodium Hydrosulphite (Hydro)
    Safety Data Sheet According to EC Regulation / 1272/2008 TSIPL/MSDS/001/18, Revision 03/03-01-2018 MATERIAL SAFETY DATA SHEET SODIUM HYDROSULPHITE (HYDRO) 1. Identification of Substance and Manufacturer 1.1 Product details Product name : Sodium hydrosulphite CAS No. : 7775 – 14 – 6 Synonyms : Sodium hydrosulphite; Sodium dithionite, Hydro Chemical Formula : Na2S2O4 Molecular weight : 174.11 g/mole Reach Registration No : 01 – 2119520510 – 57 – 001 Relevant identified uses : Reducing and Bleaching agent Bleaching : As a beaching aid in bleaching of ground wood pulp Textile : For dying and printing of Vat, Sulphur & Indigo dyes. Reduction clearing of synthetic goods. Stripping of dyes. Reducing Agent :Reduction of heavy metals such as Titanium, Chromium,Manganese, Molybdenum and Tungsten. Disulphide linkage in wool hair. Other Applications : Machine cleaning, Water treatment, Food bleaching (Sugar,Honey and gelatin),Soap Manufacturing and Glue manufacturing. 1.2 Company details TRANSPEK – SILOX INDUSTRY PRIVATE LIMITED Kalali Road, Atladra, Vadodara – 390 012, Gujarat, India Telephone : +91 265 2680401-05 Fax : +91 265 2680407 / 2680062 1.3 Emergency contact details Telephone: +91 265 2680401, Email : [email protected] Contact Person: Factory Manager www.transpek-silox.com Page 1 of 19 Safety Data Sheet According to EC Regulation / 1272/2008 TSIPL/MSDS/001/18, Revision 03/03-01-2018 MATERIAL SAFETY DATA SHEET SODIUM HYDROSULPHITE (HYDRO) 2. Hazard identification 2.1 Classification according to regulation (EC) 1272/2008 [EU-GHS/CLP] NFPA Rating HMIS Rating Health 1 1 Flammability 2 2 Reactivity 2 2 Specific hazard Not available E Classification according to EU Directives 67/548/EEC or 1999/45/EC May cause fire.
    [Show full text]
  • Zinc Pyrithione
    PATIENT INFORMATION SHEET Zinc pyrithione (Z-006) Your patch testing results indicate that you have a contact allergy to Zinc pyrithione . It is important that you familiarize yourself with this chemical and take steps to avoid coming in contact with it. i What is Zinc pyrithione and where is it found? This chemical is used as an anti-fungal, anti-bacterial and anti-seborrheic agent in many therapeutic shampoos, hair creams and other cosmetic products for the treatment of skin problems. Further research may identify additional product or industrial usages of this chemical. i What else is Zinc pyrithione called? This chemical can be identified by different names, including: 2‐mercaptopyridine ‐1‐oxide zinc salt OM ‐1563 Zinc omadine 2‐pyridinethiol ‐1‐oxide, zinc salt Pyrithione zinc Zinc pyridine ‐2‐thiol ‐1‐oxide Bis(2 ‐pyridylthio)zinc 1,1' ‐dioxide Pyridinethiol ‐1‐oxide, zinc salt Zinc, bis(1 ‐hydroxy ‐2(1H) ‐ Bis ‐(1 ‐hydroxy ‐2(1H) ‐pyridinethionato ‐ Vancide p pyridinethionato ‐O,S) ‐, (T ‐4) ‐ O,S) zinc zinc 1 ‐hydroxy ‐2‐pyridine ‐thione Zinc 2 ‐pyridinethiol ‐1‐oxide Bis(2 ‐pyridylthio)zinc, N,N' ‐dioxide Zinc ‐pyrion Zinc polyanemine Bis(1 ‐hydroxy ‐2(1H) ‐ Zinc 1 ‐hydroxypyridine ‐2‐thione Zinc PT pyridinethionato)zinc Zinc 2 ‐mercaptopyridine ‐N‐oxide Zinc, bis(2 ‐pyridylthio) ‐, N,N,' ‐dioxide Omadine zinc Zinc pyridinethione ZNPT This may not be a complete list as manufacturers introduce and delete chemicals from their product lines. THINGS YOU CAN DO TO HELP MANAGE YOUR CONTACT ALLERGY Be vigilant read the product label. Always take the time to read the ingredient listing on product packages.
    [Show full text]
  • Derivatization of Methylglyoxal for LC-ESI-MS Analysis—Stability and Relative Sensitivity of Different Derivatives
    Supplementary Derivatization of Methylglyoxal for LC-ESI-MS Analysis—Stability and Relative Sensitivity of Different Derivatives Stefan Fritzsche 1,2, Susan Billig 1,, Robby Rynek 1, Ramarao Abburi 1,3, Elena Tarakhovskaya 1,4, Olga Leuner 1,5, Andrej Frolov 6 and Claudia Birkemeyer 1,* 1 Institute of Analytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, 04103 Leipzig, Germany; [email protected] (S.F.), [email protected] (S.B.), [email protected] (R.R.), [email protected] (R.A.), [email protected] (E.T.), [email protected] (O.L.); 2 Institute of Pharmacy, Faculty of Medicine, University of Leipzig, 04103 Leipzig, Germany; 3 Department of Chemistry, Krishna University, Machilipatnam-521001, Andhra Pradesh, India; [email protected] 4 Department of Plant Physiology and Biochemistry, Saint Petersburg State University and Russian Academy of Science Library, Saint Petersburg 199034, Russian Federation; 5 Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, 165 00 Praha-Suchdol, Czech Republic; 6 Department of Biochemistry, St. Petersburg State University, Saint Petersburg 199034, Russian Federation; [email protected]; * Correspondence: [email protected]; Tel.: +49-341-973-6092 Synthesis of the permanently charged hydrazines and anilines 1.1. Materials and chemicals Acetonitrile (ACN; ROTISOLV®, ≥ 99.8%, for preparative HPLC), formic acid (> 98%, p.s.), n-hexane (ROTISOLV®, ≥ 95%, GC Ultra Grade), potassium bromate (p.a.), zinc dust (98%), methanol (ROTISOLV®, ≥ 99.95%, LC-MS Grade) and diethyl ether (ROTIPURAN®, ≥ 99.5%, p.a.) were purchased from Carl Roth, Karlsruhe, Germany. Ethanol (absolute, > 99.7%, HiPerSolv® CHROMANORM®), cyclohexane, 4-hydroxyacetanilide (p.s., ≥ 99.0%), sodium hydrogen carbonate, sodium sulfite (purum, anhydrous), potassium carbonate and hydroxide, ammonia (35%, p.a.), acetic (100%, p.a.) and sulfuric acid (98%, p.a.) were purchased from VWR Chemicals, Darmstadt, Germany.
    [Show full text]
  • Copper Pyrithione (PT 21)
    Competent Authority Report Work Programme for Review of Active Substances in Biocidal Products Pursuant to Council Directive 98/8/EC Copper pyrithione (PT 21) Applicant: TNO Quality of Life DOCUMENT III A4 Analytical methods Rapporteur Member State: Sweden Draft December 2010 Table of Contents; DOC III-A4 Section A4 Analytical Methods for Detection and Identification ........................................................ 3 General RMS comments on the Analytical Methods section .................................................................................. 3 Section A4.1/01 Determination of the pure active substance and impurities in the technical material ...... 3 Section A4.2(a)/01 Determination of residues in soil ...................................................................................... 4 Section A4.2(b)/01 Determination of residues in air ........................................................................................ 5 Section A4.2(c)/01 Determination of residues in water (drinking, sea and ground water) ............................. 9 Section A4.2(c)/02 Determination of residues in water (sea water) ............................................................... 14 Section A4.2(c)/03 Determination of residues in water (river water) ............................................................ 18 Section A4.2(c)/04 Determination of residues in sediment (and water) - ...................................................... 23 Section A 4.2(c)/05 Determination of residues in sediment (and water)........................................................
    [Show full text]
  • What's the Best Treatment for Cradle Cap?
    From the CLINIcAL InQUiRiES Family Physicians Inquiries Network Ryan C. Sheffield, MD, Paul Crawford, MD What’s the best treatment Eglin Air Force Base Family Medicine Residency, Eglin Air for cradle cap? Force Base, Fla Sarah Towner Wright, MLS University of North Carolina at Chapel Hill Evidence-based answer Ketoconazole (Nizoral) shampoo appears corticosteroids to severe cases because to be a safe and efficacious treatment of possible systemic absorption (SOR: C). for infants with cradle cap (strength of Overnight application of emollients followed recommendation [SOR]: C, consensus, by gentle brushing and washing with usual practice, opinion, disease-oriented baby shampoo helps to remove the scale evidence, and case series). Limit topical associated with cradle cap (SOR: C). ® Dowden Health Media Clinical commentary ICopyrightf parents can’t leave it be, recommend brush to loosen the scale. Although mineral oil andFor a brush personal to loosen scale use noonly evidence supports this, it seems safe Cradle cap is distressing to parents. They and is somewhat effective. want everyone else to see how gorgeous This review makes me feel more FAST TRACK their new baby is, and cradle cap can make comfortable with recommending ketocon- their beautiful little one look scruffy. My azole shampoo when mineral oil proves If parents need standard therapy has been to stress to the insufficient. For resistant cases, a cute hat to do something, parents that it isn’t a problem for the baby. can work wonders. If the parents still want to do something
    [Show full text]
  • Safety Data Sheet
    World Headquarters Hach Company P.O.Box 389 MSDS No: M00107 Loveland, CO USA 80539 (970) 669-3050 SAFETY DATA SHEET _____________________________________________________________________________ 1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION Product Name: Hydrosulfite Reagent for Total Copper Catalog Number: 2118869 Hach Company Emergency Telephone Numbers: P.O.Box 389 (Medical and Transportation) Loveland, CO USA 80539 (303) 623-5716 24 Hour Service (970) 669-3050 (515)232-2533 8am - 4pm CST MSDS Number: M00107 Chemical Name: Dithionous acid, disodium salt CAS Number: 7775-14-6 Additional CAS No. (for hydrated forms): Not applicable Chemical Formula: Na2S2O4 Chemical Family: Reducing Agent Intended Use: Laboratory Use _____________________________________________________________________________ 2. HAZARDS IDENTIFICATION GHS Classification: Hazard categories: Self Heating: Self-heat. 1 Acute Toxicity: Acute Tox. 4-Orl . Serious Eye Damage/Eye Irritation:Eye Irrit. 2 Specific Target Organ Toxicity - Single Exposure: STOT SE 3 Hazardous to the Aquatic Environment: Aquatic Chronic 3 GHS Label Elements: DANGER Hazard statements: Self-heating: maycatch fire. Harmful if swallowed. Causes serious eye irritation. May cause respiratory irritation. Harmful to aquatic life with long lasting effects. Precautionary statements: Avoid breathing dust/fume/gas/mist/vapours/spray. Handle environmental release according to local, state, federal, provincial requirements. Wear eye protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention. IF INHALED: Remove victim/person to fresh air and keep at rest in a position comfortable for breathing. Call a POISON CENTER or doctor/physician if you feel unwell. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.
    [Show full text]
  • Development and Validation of UV Spectroscopic Method for Estimation of Climbazole in Climbazole Shampoo
    RESEARCH ARTICLE Am. J. PharmTech Res. 2020; 10(01) ISSN: 2249-3387 Journal home page: http://www.ajptr.com/ Development and Validation of UV Spectroscopic Method for Estimation of Climbazole in Climbazole Shampoo Rajashree N. Patil*1, Pratiksha A. Patil1, Swapnil R. Patil2, Amol Sawale2 1.Department of Pharmaceutical Chemistry, Arunamai College of Pharmacy, Mamurabad, North Maharashtra University, Jalgaon (MH) INDIA 425002. 2.Department of Pharmaceutics and Quality Assurance, Vidya Bharati College of Pharmacy, Amravati University, Amravati (MH) INDIA 444602. ABSTRACT To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Climbazole in Climbazole shampoo. The drug is freely soluble in organic solvents such as Chloroform and Methanol. The drug was identified in terms of solubility studies and on the basis of melting point done on Melting Point Apparatus of Equiptronics. It showed absorption maxima were determined in Methanol: Water (50:50). The drug obeyed the Beer’s law and showed good correlation of concentration with absorption which reflect in linearity. The UV spectroscopic method was developed for estimation of Climbazole in shampoo dosage form and also validated as per ICH guidelines. The drug is freely soluble in organic solvents such as Methanol, Chloroform. So, the Analytical Grade Methanol: Water is used as a diluent in equal proportion for method. The melting point of Climbazole was found to be 93-94˚C (uncorrected). It showed absorption maxima 256 nm in Methanol: Water (50:50). On the basis of absorption spectrum the working concentration was set on 6µg/ml (PPM). The linearity was observed between 2-10 μg/ml (PPM).
    [Show full text]
  • An Approach to the Influence of Particle Size Distribution of Leuco Vat Dye Converted by a Reducing Agent
    Fibers and Polymers 2006, Vol.7, No.2, 164-168 An Approach to the Influence of Particle Size Distribution of Leuco Vat Dye Converted by a Reducing Agent Woo Sub Shim*, Jung Jin Lee1, and Renzo Shamey Fiber and Polymer Science Program, North Carolina State University, Raleigh, NC 27695-8301, USA 1Department of Textile Engineering, Dankook University, Seoul 140-714, Korea (Received January 25, 2006; Revised March 23, 2006; Accepted April 2, 2006) Abstract: Three vat dyes have been applied to regular viscose rayon and their dyeing and wash fastness properties were eval- uated. Particle size determination was undertaken to obtain information about the size of dye particles converted by a reduc- ing agent, to see if dye particle size has an affect on dyeing properties of regular viscose rayon. It is observed that viscose rayon exhibits more dyeability with reducing agent concentrations between 5-7.5 g/l. Also, we found that the vat dyeing sys- tem is greatly affected by the particle size of the vat dye converted to leuco form by a reducing agent. Keywords: Particle size, Particle size distribution, Reducing agent, Viscose rayon, Vat dye, Dyeing properties, Wash fastness Introduction (Na2S2O4). The name “hydro” is commonly used for sodium hydrosulfite in the dyehouse. Reduction is done in the presence Vat dyes are used to dye cellulosic fibers in relatively dull of sodium hydroxide (NaOH) which is usually called caustic. shades requiring good fastness. They are insoluble in water The quantities of caustic and hydro required depend on how and cannot be used directly for dyeing cellulosic fibers.
    [Show full text]
  • 12 Env/Jm/Mono(2004)12
    Unclassified ENV/JM/MONO(2004)12 Organisation de Coopération et de Développement Economiques Organisation for Economic Co-operation and Development 24-Jun-2004 ___________________________________________________________________________________________ _____________ English - Or. English ENVIRONMENT DIRECTORATE JOINT MEETING OF THE CHEMICALS COMMITTEE AND Unclassified ENV/JM/MONO(2004)12 THE WORKING PARTY ON CHEMICALS, PESTICIDES AND BIOTECHNOLOGY OECD SERIES ON EMISSION SCENARIO DOCUMENTS Number 7 EMISSION SCENARIO DOCUMENT ON TEXTILE FINISHING INDUSTRY English - Or. English JT00166691 Document complet disponible sur OLIS dans son format d'origine Complete document available on OLIS in its original format ENV/JM/MONO(2004)12 OECD Environmental Health and Safety Publications Series on Emission Scenario Documents No. 7 EMISSION SCENARIO DOCUMENT ON TEXTILE FINISHING INDUSTRY Environment Directorate Organisation for Economic Co-operation and Development June 2004 3 ENV/JM/MONO(2004)12 ABOUT THE OECD The Organisation for Economic Co-operation and Development (OECD) is an intergovernmental organisation in which representatives of 30 industrialised countries in North America, Europe and the Pacific, as well as the European Commission, meet to co-ordinate and harmonize policies, discuss issues of mutual concern, and work together to respond to international problems. Most of the OECD's work is carried out by more than 200 specialised Committees and subsidiary groups composed of Member country delegates. Observers from several countries with
    [Show full text]
  • Research Paper on Antidandruff Formulation and Evaluation of Antidandruff Shampoo Minal S
    ISSN XXXX XXXX © 2019 IJESC Research Article Volume 9 Issue No. 6 Research Paper on Antidandruff Formulation and Evaluation of Antidandruff Shampoo Minal S. Agrawal B. Tech Student Department of Cosmetics Smt. Meharbanu College of Science and Commerce, Akola, India Abstract: Shampooing is the most common form of hair treatment to remove dirt, grease, and debris from the hair, scalp and other parts of body without damaging the natural gloss of hair and to keep the hair fragrant, lustrous, soft and manageable. The shampoo is prepared by using Antidandruff Agents Zinc Pyrithione. The other ingredients used are sodium lauryl sulphate as surfactant, urea as solubilizing agent, sodium EDTA as chelating agent, guar gum as foam stabilizer and thickening agent, distilled water is used as vehicle. It was evaluated for various test and the ranges were in acceptable limits. The Ph range was 6.5 to 9.0, physical appearance was off white color. percentage (%) of zinc contents ranges from 09.3 to 11.3%,particle size was in the range of 90%<1 Micron, Assay of (% ZPT) was 48% to 50%.The present research emphasizes about the preparation and evaluation of synthetic antidandruff cosmetic shampoo. Keywords: Antidandruff, Zinc Pyrithi one. I.0. INTRODUCTION sulphate, triethanol lauryl sulphate. Secondary surfactant e.g. dialkyl sulphosuccinates, monoalkyl sulphosuccinates. The first antidandruff shampoo containing Zinc Pyrithione was Germicides and Antidandruff E.g. Zinc Pyrithione. launched almost four decades ago. Since then Zinc Pyrithione Conditioning agents e.g. fatty substance like lanolin, oils. bactericide-fungicide has become the world’s leading Pearlescent agent e.g.
    [Show full text]
  • Ge 1/9 Safety Data Sheet Acc
    Page 1/9 Safety Data Sheet acc. to ISO/DIS 11014 Printing date 09/03/2014 Reviewed on 09/03/2014 1 Identification · Product identifier · Trade name: Vario Ferro F10, 25 ml · Catalogue number: 00530569, (4)530560, 530563, 00530579, (4)530570 · Relevant identified uses of the substance or mixture and uses advised against: No further relevant information available. · Application of the substance / the mixture: Reagent for water analysis · Manufacturer/Supplier: Tintometer Inc. 6456 Parkland Drive Sarasota, FL 34243 USA phone: (941) 756-6410 fax: (941) 727-9654 www.lovibond.us Made in Germany · Emergency telephone number: Chemtrec® : (US & Canada) 800-424-9300 (International) +1 (703) 527-3887 * 2 Hazard(s) identification · Classification of the substance or mixture US-GHS ~ GHS05 Corrosion Eye Dam. 1 H318 Causes serious eye damage. ~ GHS07 Acute Tox. 4 H302 Harmful if swallowed. Skin Sens. 1 H317 May cause an allergic skin reaction. · Classification according to Directive 67/548/EEC or Directive 1999/45/EC ?@G Xn; Harmful R22: Harmful if swallowed. ?@G Xi; Irritant R41: Risk of serious damage to eyes. R31: Contact with acids liberates toxic gas. · Label elements · GHS label elements The product is classified and labeled according to the Globally Harmonized System (GHS). · Hazard pictograms GHS05, GHS07 · Signal word Danger · Hazard-determining components of labeling: sodium dithionite disodium disulphite methenamine · Hazard statements H302 Harmful if swallowed. H318 Causes serious eye damage. H317 May cause an allergic skin reaction. (Contd. on page 2) USA 39.2.3.5 Page 2/9 Safety Data Sheet acc. to ISO/DIS 11014 Printing date 09/03/2014 Reviewed on 09/03/2014 Trade name: Vario Ferro F10, 25 ml (Contd.
    [Show full text]
  • Safety Data Sheet
    SAFETY DATA SHEET Preparation Date: 8/12/2016 Revision Date: 8/11/2016 Revision Number: G1 1. IDENTIFICATION Product identifier Product code: S1809 Product Name: SODIUM HYDROSULFITE, REAGENT Other means of identification Synonyms: Disodium dithionite Disodium hydrosulfite Dithionous acid, disodium salt Sodium dithionite Sodium hyposulfite Sodium sulfoxylate CAS #: 7775-14-6 RTECS # Not available CI#: Not available Recommended use of the chemical and restrictions on use Recommended use: In the textile industry, it is primarily used as a reducing agent for vat dyes and sulfur containing dyes and for removal of pigments on textiles. Pulp and paper bleaching. Dyestripping applications. Uses advised against No information available Supplier: Spectrum Chemical Mfg. Corp 14422 South San Pedro St. Gardena, CA 90248 (310) 516-8000. Order Online At: https://www.spectrumchemical.com Emergency telephone number Chemtrec 1-800-424-9300 Contact Person: Martin LaBenz (West Coast) Contact Person: Ibad Tirmiz (East Coast) 2. HAZARDS IDENTIFICATION Classification This chemical is considered hazardous according to the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200) Considered a dangerous substance or mixture according to the Globally Harmonized System (GHS) Serious eye damage/eye irritation Category 2B Specific target organ toxicity (single exposure) Category 3 Self-heating substances and mixtures Category 1 Label elements Danger Hazard statements Product code: S1809 Product name: SODIUM 1 / 12 HYDROSULFITE, REAGENT Causes eye irritation May cause respiratory irritation Self-heating: may catch fire Hazards not otherwise classified (HNOC) Reacts with water to release toxic and/or corrosive gases Reacts with water to evolve heat Other hazards May be harmful if swallowed Precautionary Statements - Prevention Wash face, hands and any exposed skin thoroughly after handling Avoid breathing dust/fume/gas/mist/vapors/spray Use only outdoors or in a well-ventilated area Keep cool.
    [Show full text]