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CURRICULUM VITAE Olga Stakhovskaya e-mail: [email protected]; [email protected]; [email protected];

EDUCATION: 2015 University of Maryland, College Park Au.D., Doctoral Program in 2002 Russian State Medical University, Moscow, Russia Ph.D., Medical Sciences, Pediatric otolaryngology, cisplatin 1997 Russian State Medical University, Moscow, Russia M.D., Pediatrics, not licensed in the US

POSTDOCTORAL EDUCATION/EMPLOYMENT: 2016-present Dept. of and Speech Sciences, University of Maryland, College Park, MD Assoc. Research Scientist 2015-present National Military Audiology and Speech Pathology Center, Walter Reed National Military Medical Center, Bethesda, MD Research Engineer II/Research Audiologist (Contractor: LRRI) 2014-2015 Audiology, Otolaryngology Branch, NIDCD, National Institutes of Health, Bethesda, MD Audiology Clinical Externship (director: Carmen Brewer, Ph.D.) 2011-2014 Department of Hearing and Speech Sciences, University of Maryland, College Park, MD Graduate Research Assistant, Auditory Perception and Modeling Lab (director: Matthew Goupell, Ph.D.) 2007-2011 Dept. of Otolaryngology - Head and Neck Surgery, University of California San Francisco, San Francisco, CA Associate/Assistant Specialist, Saul and Ida Epstein Laboratory for Auditory Physiology and Genetics 2

Other Employment/Education: 2004-2007 Dept. of Otolaryngology - Head and Neck Surgery, University of California San Francisco, San Francisco, CA Postdoctoral Fellow, Saul and Ida Epstein Laboratory for Auditory Physiology and Genetics (director: Patricia Leake, Ph.D.) 2001-2002 Morozov’s Children Hospital, Moscow, Russia Pediatric Otolaryngologist 1999-2002 N.N.Blokhin Russian Cancer Research Center, Moscow, Russia Research Fellow, Pediatric Oncology 1997-2002 Dept. of Otolaryngology, Russian State Medical University, Moscow, Russia Residency/Ph.D. program, Pediatric Otolaryngology PROFESSIONAL ORGANIZATIONS  2004-present Association of Research in Otolaryngology (ARO)  2013-present American Auditory Society (AAS)  2015-present American Speech-Language-Hearing Association (ASHA)  2015-present American Cochlear Implant Alliance (ACIA)

JOURNAL-REVIEWING ACTIVITIES:

 Audiology-Neurotology  and Hearing  IEEE Transactions on Biomedical Engineering  Annals of Otology, Rhinology & Laryngology  Hearing Research

RESEARCH GRANTS and AWARDS:

 Deafness Research Foundation, 2009-2011 ($50 000). Estimating optimum insertion depth for the HiFocus 1J and Contour Advanced electrode arrays in individual human cochleae based on high-resolution CT images.  AAA Student Summer Research Fellowship, Summer 2013 ($2500)  Invited speaker, Academy Research Conference, AAA meeting in Anaheim, CA, April 2013  Student Travel Award, CIAP meeting, Lake Tahoe, CA, July 2013 3

 Student Travel Award, AAS meeting, Scottsdale, AZ, March 2015  Outstanding AuD Student Award, University of Maryland, May 2015

RESEARCH EXPERIENCE: Workshops and Courses Attended 2014-2015 Introduction to the Principles and Practices of Clinical Trials, National Institutes of Health 2006, April Neurostereology Workshop, Marine Biological Laboratory, Woods Hole, Massachusetts Projects and responsibilities 2016-present Research Scientist, Dept. of Hearing and Speech Sciences, University of Maryland, College Park, MD

 Studying of binaural hearing in individuals with bilateral cochlear implants.

2015-present Research Audiologist/Engineer, WRNMC (Labs of Dr. Grant, Dr. Bernstein).

 Experimental support of clinical studies of hearing deficits in blast-exposed individuals.  Studying of binaural advantages in individuals with single-sided deafness and a cochlear implant. Skills and responsibilities: o Development of a research interface to study the auditory and cognitive deficits in blast-exposed individuals (Matlab). o Development of psychoacoustic experiments to study speech understanding in individuals with single-sided deafness using alternative cochlear implant maps. o Mapping of cochlear implants using alternative frequency allocation maps to optimize the binaural performance in individuals with single-sided deafness and a cochlear implant. o Collection, analysis and interpretation of complex auditory responses. o Recording of electrophysiological responses in selective attention tasks in blast-exposed and non-blast exposed individuals. o Development and support of IRB protocols, participant recruitment, consenting, and handling of protected health information (PHI).

2014-2015 Audiology Extern, NIH (Director: Dr. Brewer).  Participated in clinical research projects 4

o Audiological characteristics in patients with rare diseases and syndromes. o Stability of OAEs and their value in predicting ototoxicity-related changes in hearing thresholds. o Development of the protocol for determining the ABR thresholds in children with Niemann-Pick disease. o Audiological profile in patients with Alstrom syndrome.

2011-2014 Graduate Research Assistant, Auditory Processing and Modeling Lab, University of Maryland, College Park (Director – Dr. Goupell).  Participated in projects to study the benefits of bilateral cochlear implantation. Skills and responsibilities: o Programming of research interface for data collection using Matlab and direct stimulation of the cochlear implants. o Mapping of cochlear implants using research and clinical interface. o Studying lateralization of multi-frequency sounds in normal-hearing and cochlear implant listeners. o Modeling of multi-frequency ILDs in normal-hearing and cochlear implant listeners. 2007-2011 Assistant/Associate Specialist, Department of Otolaryngology, Epstein Lab, UCSF (Director – Dr. Leake).  Studied the role of the intracochlear infusion of neurotrophic factors (BDNF) on the survival of the spiral in cats with various deafness histories.  Tested the reliability of specific anatomical landmarks in predicting the required insertion depth of the intracochlear electrode arrays in the human temporal bones.  Studied the characteristics of monopolar and bipolar electrical stimulation in the central nucleus of the in guinea pigs.

Skills and responsibilities: o Dissection of human temporal bones, insertion of intracochlear arrays (Cochlear Contour Advance, HiFocus J1), micro-computer tomography of the human temporal bones with inserted arrays, 3D reconstruction of scanned images (AMIRA software, NIH Image). o Live surgery under prolonged anesthesia in experimental animals (cats, guinea pigs) to expose structures (, , inferior colliculus) for cochlear implantation and electrophysiological recordings. o Chronic intracochlear stimulation via cochlear implant in live animals, monitoring of auditory brainstem responses, and collection of electrophysiological data. 5

o Light and electron microscopy, imaging and stereological analysis. o Data analysis using Matlab, SPSS, Sigmaplot, Excel, Canvas, Photoshop, NIH Image, AMIRA, Graphpad Prism.

2004-2007 Post-doctoral fellow, Department of Otolaryngology, Epstein Lab, UCSF (director: Dr. Leake).  Studied the role of onset of deafness and developmental critical/sensitive periods in the adaptive changes in the auditory system following auditory deprivation and/or intracochlear electric stimulation in cats.  Developed a spiral ganglion frequency map for the human cochlea based on the trajectories of the auditory fibers from the into the spiral ganglion.

Skills and responsibilities: o Histological analysis; harvesting the tissues from experimental animals, dissection, sectioning (thin, ultrathin, frozen), mounting, embedding, staining of biological specimens (organ of Corti, spiral ganglion, cochlear nucleus, inferior colliculus) for histological evaluation. o Conduction of ABRs and Electric ABRs in cats and guinea pigs under isoflurane anesthesia. o Single unit and multi-electrode probe recordings from spiral ganglion and inferior colliculus in cats.

1997-2002 Ph.D. student  Investigation of ototoxic effects of different cisplatin-based chemotherapy protocols in pediatric patient.

Related skills and responsibilities: o Audiological evaluation of pediatric patients and monitoring for signs of early ototoxicity after different regimens of cisplatin/carboplatin-based chemotherapy. o Modeling of cisplatin-induced ototoxicity in guinea pigs and investigation of the otoprotective effects of sodium thiosulfate and unithiol in vivo.

DISSERTATION 6

Stakhovskaya, O.A. Cisplatin ototoxicity in treating malignant neoplasms (clinical and experimental study), Ph.D. dissertation, 2002, Moscow, 127 pp. (in Russian). PEER-REVIEWED PUBLICATIONS: Book chapter: Leake, P.A., Stakhovskaya, O., Rebscher, S. J. (2013). “Effects of early-onset deafness in the developing auditory system”, in Deafness (Editors: Kral, A., Fay, R.R., Popper, A.N., Springer – New-York, Heidelberg, Dordrecht, London). Peer-reviewed articles: 1. Bogomil'skii, M.R., Poliakov, V.G., Sapozhnikov, Ya.M., Lebedeva, L.I., Stakhovskaia, O.A. (2002). Cisplatin ototoxicity in children with malignant tumors. Detskaya Onkologia, 1: 10-12. (in Russian). 2. Bogomil'skii, M.R., D'iakonova, I.N., Poliakov, V.G., Rakhmanova, I.V., Stakhovskaia, O.A., Tikhomirov, A.M. (2002). Ototoxic effect and its prevention in administration of cisplatinum. Vestnik Otorinolaringologii, 2: 7-10. (in Russian). 3. Sridhar, D., Stakhovskaya, O., Leake, P. (2006). A frequency-position function for the human cochlear spiral ganglion. Audiology & Neurotology, 11(suppl): 16-20. 4. Stakhovskaya, O., Sridhar, D., Bonham, B., Leake P. (2007). Frequency map for the human cochlear spiral ganglion: implications for cochlear implants. J Assoc Res Otolaryngol., 8:220-233. 5. Jones, T.A., Leake, P.A., Snyder, R.L., Stakhovskaya, O., Bonham, B. (2007). Spontaneous discharge patterns in cochlear spiral ganglion cells prior to the onset of hearing in cats. J Neurophysiol., 98(4):1898-908. 6. Leake, P.A., Stakhovskaya, O., Hradek, G.T. (2008). Factors Influencing Neurotrophic Effects of Electrical Stimulation in the Deafened Developing Auditory System. (2008) Hear Res., 242(1-2): 86-99. 7. Stakhovskaya, O., Hradek, G.T., Snyder, R.L., Leake, P.A. (2008). Effects of Age at Onset of Deafness and Electrical Stimulation on the Developing Cochlear Nucleus. Hear Res., 243(1-2): 69-77. 8. Leake, P.A., Hradek, G.T., Hetherington, A.M., Stakhovskaya, O. (2011). Brain-derived neurotrophic factor (BDNF) promotes cochlear spiral ganglion cell survival and function in deafened, developing cats. J Comp Neurol., 519(8): 1526-45. 9. Schoenecker, M.C., Bonham, B.H., Stakhovskaya, O.A., Snyder, R.L., Leake, P.A. (2012). Monopolar intracochlear pulse trains selectively activate the inferior colliculus. J Assoc Res Otolaryngol., 13(5): 655-72. 10. Leake, P.A., Stakhovskaya, O., Hetherington, A., Rebscher, S.J., Bonham, B. (2013). Effects of Brain-Derived Neurotrophic Factor (BDNF) and Electrical Stimulation on Survival and Function of Cochlear Spiral Ganglion Neurons in Deafened, Developing Cats. J Assoc Res Otolaryngol., 14(2): 187-211. 11. Kandathil, C., Stakhovskaya, O., Leake, P. (2016). Effects of Brain-Derived Neurotrophic Factor (BDNF) on the Cochlear Nucleus in Cats Deafened as Neonates. Hear Res., 342: 134-143. 12. Stakhovskaya, O.A., Goupell, M.J. (2017). Lateralization of Interaural Level Differences with Multiple Electrode Stimulation in Bilateral Cochlear-Implant Listeners. Ear Hear, 38(1): 22-38.