Supplementary File S6b. Maximum likelihood phylogram inferred from nrITS of the investigated dataset integrated with 251 sequences of the subtribe Agrostidinae

(Soreng et al. 2017) retrieved from GenBank. Bootstrap support (>50) is indicated on the corresponding branches. Colouration refers to different genera.

Tree scale: 0.01

KU883475 Briza humilis 100 KU883474 Briza humilis HG797493 rupestris

85 KX872969 Calamagrostis erectifolia FJ377653 Calamagrostis eriantha 100 FJ377641 Calamagrostis minima 60 FJ377645 Calamagrostis pringlei HG797492 Calamagrostis rigida

100 HG797491 Calamagrostis filifolia HG797496 Calamagrostis tarmensis 93 KX873039 Calamagrostis brevifolia HG797503 Calamagrostis tarmensis 87 85 HG797495 Calamagrostis tarmensis MF348279 Calamagrostis brevifolia HG797494 Calamagrostis tarmensis KX872956 Calamagrostis coarctata

84 DQ539590 Ammophila arenaria 100 KX872915 Ammophila arenaria KX872916 Ammophila arenaria 83 70 KU883483 Ammophila arenaria 76 KP711072 Calamagrostis epigeios KX872962 Calamagrostis emodensis KX873018 Calamagrostis varia

69 KU883492 Calamagrostis varia KX873017 Calamagrostis varia KX873019 Calamagrostis varia Colored ranges KX872940 Calamagrostis arundinacea 67 KX872941 Calamagrostis arundinacea KX872997 Calamagrostis rivalis

Ammophila 99 KX872998 Calamagrostis rivalis FJ821786 Calamagrostis pseudophragmites Briza 94 88 KX872994 Calamagrostis pseudophragmites subsp tartarica Calamagrostis KX872993 Calamagrostis pseudophragmites subsp tartarica 66 KP711071 Calamagrostis pseudophragmites KX872991 Calamagrostis pseudophragmites KX872992 Calamagrostis pseudophragmites EU887016 Calamagrostis pseudophragmites Podagrostis MF348276 Agrostis rosei KX873015 Calamagrostis tianschanica KX872977 Calamagrostis lapponica 60 KX873005 Calamagrostis stricta subsp groenlandica KX873004 Calamagrostis stricta subsp groenlandica KX872957 Calamagrostis deschampsioides 57 FJ377617 Calamagrostis purpurascens 83 FJ377651 Calamagrostis purpurascens KX872944 Calamagrostis bolanderi 100 KX872943 Calamagrostis bolanderi KX873136 Podagrostis aequivalvis KX873021 Calamagrostis villosa KX873020 Calamagrostis villosa KX872952 Calamagrostis canescens KX872951 Calamagrostis canescens KX872950 Calamagrostis canescens KX872982 Calamagrostis nutkaensis 87 KX872983 Calamagrostis nutkaensis KX872978 Calamagrostis lapponica KX873085 Calamagrostis lahulensis KX872947 Calamagrostis canadensis subsp canadensis KX872949 Calamagrostis canadensis subsp langsdorffii FJ377631 Calamagrostis canadensis var langsdorffii

51 FJ377632 Calamagrostis canadensis 56 FJ377630 Calamagrostis canadensis var langsdorffii KX872996 Calamagrostis purpurea KX873022 Calamagrostis villosa HQ114381 Calamagrostis canadensis

88 HQ114382 Calamagrostis canadensis KX872937 Calamagrostis angustifolia subsp angustifolia FJ377629 Calamagrostis canadensis var langsdorffii KX872945 Calamagrostis brachytricha 98 KX872942 Calamagrostis arundinacea 74 HQ600531 Calamagrostis arundinacea 74 KX872959 Calamagrostis distantiflora 93 GQ266675 Calamagrostis arundinacea KX872967 Calamagrostis epigeios subsp glomerata

57 KX873007 Calamagrostis stricta subsp inexpansa 80 KX873009 Calamagrostis neglecta FJ377659 Calamagrostis stricta subsp stricta FJ377640 Calamagrostis lapponica KX873014 Calamagrostis neglecta

53 KX873025 Calamagrostis gracilescens 62 KX873012 Calamagrostis neglecta 65 FJ377644 Calamagrostis poaeoides FJ377639 Calamagrostis lapponica KX872946 Calamagrostis cainii 99 FJ377627 Calamagrostis cainii KX872988 Calamagrostis porteri subsp porteri

60 FJ377623 Ammophila champlainensis KX872986 Calamagrostis pickeringii KJ598966 Agrostis alpina KX872974 Calamagrostis koelerioides 61 KX872970 Calamagrostis foliosa KX872972 Calamagrostis holciformis KX872938 Calamagrostis anthoxanthoides subsp laguroides

69 KX872939 Calamagrostis anthoxanthoides KX872955 Calamagrostis chalybaea KX873002 Calamagrostis sesquiflora 68 KX873001 Calamagrostis sesquiflora

54 KX873000 Calamagrostis sesquiflora KX872973 Calamagrostis howellii 100 FJ377638 Calamagrostis howellii KX872971 Calamagrostis guatemalensis FJ377652 Calamagrostis rubescens FJ377654 Calamagrostis scopulorum

63 FJ377655 Calamagrostis scopulorum AY705904 Lachnagrostis billardierei

96 KX873137 72 FJ377671 Polypogon australis KU883524

50 KX873141 Polypogon interruptus 98 KX873144 Polypogon viridis 99 KX873143 Polypogon viridis

52 KX873145 Polypogon viridis DQ146796 Polypogon viridis

88 DQ146770 Agrostis exarata

68 DQ146769 Agrostis exarata

59 KX872901 Agrostis exarata 83 KX872902 Agrostis exarata KP135427 Polypogon fugax KP296104 KP135428 Polypogon fugax KU883525 KF713280 Polypogon monspeliensis KF713277 Polypogon fugax KF713274 Polypogon fugax 95 JN040628 Polypogon monspeliensis

60 JN040629 Polypogon monspeliensis HQ600511 Polypogon fugax MF063642 Polypogon fugax LR699049 Polypogon monspeliensis MF063644 Polypogon fugax

51 KX873142 Polypogon monspeliensis 64 MF063643 Polypogon fugax 56 KF713273 Polypogon fugax JN040630 Polypogon monspeliensis

70 JN040631 Polypogon monspeliensis JN040624 Polypogon monspeliensis 88 JN040625 Polypogon monspeliensis DQ146794 Polypogon monspeliensis DQ146795 Polypogon monspeliensis KF850575 Polypogon monspeliensis KX872979 Calamagrostis llanganatensis

61 KX872953 Calamagrostis carchiensis KU883502 Gastridium phleoides KP296126 Triplachne nitens 90 100 DQ336816 Triplachne nitens

62 LR588244 Triplachne nitens LR588232 Gastridium phleoides 4 LR588236 Gastridium phleoides 11 LR588229 Gastridium phleoides 1 LR588215 3 LR588216 Gastridium ventricosum 4 100 LR588239 Gastridium phleoides 14 LR588237 Gastridium phleoides 12 KU883503 Gastridium ventricosum LR588231 Gastridium phleoides 3 LR588240 Gastridium phleoides 15

62 DQ336817 Gastridium ventricosum LR588228 Gastridium ventricosum 21 82 LR588234 Gastridium phleoides 9

63 LR588238 Gastridium phleoides 13 LR588230 Gastridium phleoides 2 LR588233 Gastridium phleoides 7 LR588243 Gastridium lainzii 1 LR588226 Gastridium ventricosum 18 LR588219 Gastridium ventricosum 9 LR588214 Gastridium ventricosum 2 LR588221 Gastridium ventricosum 11 LR588225 Gastridium ventricosum 16 LR588227 Gastridium ventricosum 19 LR588213 Gastridium ventricosum 1 LR588218 Gastridium ventricosum 8 LR588220 Gastridium ventricosum 10 LR588222 Gastridium ventricosum 12 LR588217 Gastridium ventricosum 7 LR588235 Gastridium phleoides 10 LR606134 Gastridium lainzii 2 LR588223 Gastridium ventricosum 13

67 LR588224 Gastridium ventricosum 15 LR588241 Gastridium scabrum 1 69 LR588242 Gastridium scabrum 2

84 LR594548 Gastridium scabrum 3 DQ539592 Agrostis truncatula subsp commista KU136318 Agrostis barbuligera 100 KU136317 Agrostis barbuligera var longipilosa KX640977 Lachnagrostis lyallii KX872908 Agrostis mertensii GQ324467 Agrostis mertensii AY705903 Lachnagrostis leptostachys FJ042866 Agrostis canina FJ042864 Agrostis canina FJ042856 Agrostis canina 59 FJ042855 Agrostis canina 99 FJ042849 Agrostis canina

55 FJ042859 Agrostis canina

60 FJ042847 Agrostis canina AY705881 Agrostis personata AY705907 Lachnagrostis ammobia JN040622 Agrostis stolonifera 99 JN040623 Agrostis stolonifera 88 JN040615 Agrostis stolonifera 50 JN040614 Agrostis stolonifera HQ600521 Agrostis clavata

65 FJ766144 Agrostis clavata var nukabo FJ042846 Agrostis stolonifera FJ042836 Agrostis stolonifera 64 FJ042826 Agrostis stolonifera FJ821787 Agrostis gigantea FJ821782 Agrostis stolonifera KM999951 Agrostis gigantea KF713269 Agrostis clavata 83 HQ600526 Agrostis hyemalis 54 KF713268 Agrostis clavata DQ539593 Agrostis fasciculatus 77 FJ042829 Agrostis capillaris DQ539591 Agrostis castellana FJ042821 Agrostis capillaris 50 KU883481 Agrostis castellana

50 DQ146768 Agrostis castellana FJ042824 Agrostis capillaris 52 94 FJ042819 Agrostis capillaris LR699050 Agrostis castellana FJ042842 Agrostis capillaris FJ042841 Agrostis capillaris FJ377621 Agrostis scabra DQ146767 Agrostis capillaris DQ146772 Agrostis gigantea 51 DQ146771 Agrostis gigantea KX873140 Polypogon elongatus KX873139 Polypogon elongatus 68 98 KX873138 Polypogon elongatus FJ042872 Agrostis vinealis FJ042871 Agrostis vinealis 61 FJ042804 Agrostis vinealis

80 FJ042803 Agrostis vinealis FJ377619 Agrostis meyenii DQ146775 Agrostis pallens DQ146773 Agrostis idahoensis DQ146784 Agrostis stolonifera DQ146774 Agrostis mertensii KX872909 Agrostis scabra KU883482 Agrostis tolucensis 95 JF904802 Agrostis tolucensis DQ146776 Agrostis scabra 95 KX872907 Agrostis hallii KX872913 Agrostis tolucensis

89 KX872897 Agrostis breviculmis 88 KX872898 Agrostis breviculmis KX872904 Agrostis gelida

74 KX872912 Agrostis tolucensis 92 KX872903 Agrostis foliata

93 KX872906 Agrostis tolucensis