MOLECULAR AND CELLULAR BIOLOGY Volume 10 September 1990 No. 9

GENE EXPRESSION Tc, an Unusual Promoter Element Required for Constitutive of the HIS3 Gene. Subramony Mahadevan and Kevin Struhl...... 4447 4455 N6-Methyladenosine Residues in an Intron-Specific Region of Prolactin Pre- mRNA. Simon M. Carroll, Prema Narayan, and Fritz M. Rottman...... 4456-4465 Identification and Characterization of the Small Nuclear Ribonucleoprotein Parti- cle D' Core Protein. Janet Andersen, Robert J. Feeney, and Gary W. Zieve . 4480-4485 Expression and Function of a Human Initiator tRNA Gene in the Yeast Saccharo- myces cerevisiae. Marilyn A. Francis and Uttam L. Rajbhandary ...... 4486-4494 Transcriptional Control of the Rat Hepatic CYP2E1 Gene. Takaki Ueno and Frank J. Gonzalez ...... 4495-4505 Synthesis of Functional mRNA in Mammalian Cells by T3 RNA Polymerase. Youwen Zhou, Thomas J. Giordano, Russell K. Durbin, and William T. McAllister ...... 4529-4537 Identification and Characterization of Transcriptional Arrest Sites in Exon 1 of the Human Adenosine Deaminase Gene. Zhi Chen, Michael L. Harless, David A. Wright, and Rodney E. Kellems...... 4555-4564 Domain Swapping Reveals the Modular Nature of Fos, Jun, and Creb Proteins. Lynn J. Ransone, Penny Wamsley, Kimberlin L. Morley, and Inder M. Verma . 4565-4573 nt' Glucocorticoid Receptor Transcripts Lack Sequences Encoding the Amino- Terminal Transcriptional Modulatory Domain. Ellen S. Dieken, Eckart U. Meese, and Roger L. Miesfeld ...... 4574 4581 Selection and Analysis of Galactose Metabolic Pathway Variants of a Mouse Liver Cell Line. Kenneth S. Zaret and Kimberly A. Stevens ...... 4582-4589 A Novel Small-Subunit Ribosomal Protein of Yeast Mitochondria That Interacts Functionally with an mRNA-Specific Translational Activator. Thomas W. McMullin, Pascal Haffter, and Thomas D. Fox...... 4590-4595 Functional Analysis and Nucleotide Sequence of the Promoter Region of the Mu- rine hck Gene. Peter Lock, Edouard Stanley, Douglas A. Holtzman, and Ashley R. Dunn ...... 4603-4611 Phosphorylated Forms of GAL4 Are Correlated with Ability To Activate Tran- scription. Lawrence M. Mylin, Mark Johnston, and James E. Hopper.... 4623-4629 Evi-2, a Common Integration Site Involved in Murine Myeloid Leukemogenesis. Arthur M. Buchberg, Hendrick G. Bedigian, Nancy A. Jenkins, and Neal G. Copeland...... 4658-4666 Spacer Promoters Are Orientation-Dependent Activators of Pre-rRNA Transcrip- tion in Drosophila melanogaster. Giovanna Grimaldi, Paola Fiorentini, and Pier Paolo Di Nocera...... 4667-4677 Regulatory Elements Controlling Pituitary-Specific Expression of the Human Pro- lactin Gene. Bernard Peers, Marianne L. Voz, Philippe Monget, Marianne Mathy-Hartert, Monique Berwaer, Alexandra Belayew, and Joseph A. Martial ...... 4690-4700

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Expression of the Neurotransmitter-Synthesizing Enzyme Glutamic Acid Decar- boxylase in Male Germ Cells. Hakan Persson, Markku Pelto-Huikko, Madis Metsis, Olof Soder, Stefan Brene, Sven Skog, Tomas H6kfelt, and E. Martin Ritzen...... 4701-4711 Characterization of a Complex Glucocorticoid Response Unit in the Phosphoenol- pyruvate Carboxykinase Gene. Enyu Imai, Per-Eric Stromstedt, Patrick G. Quinn, Jan Carlstedt-Duke, Jan-Ake Gustafsson, and Daryl K. Granner 4712-4719 Regulation of the Human T-Cell Receptor a Gene Enhancer: Multiple Ubiquitous and T-Cell-Specific Nuclear Proteins Interact with Four Hypomethylated En- hancer Elements. I-Cheng Ho and Jeffrey M. Leiden...... 4720-4727 The RPC31 Gene of Encodes a Subunit of RNA Poly- merase C (III) with an Acidic Tail. Christine Mosrin, Michel Riva, Monica Beltrame, Evelyne Cassar, Andre Sentenac, and Pierre Thuriaux ...... 4737-4743 The N-Terminal Tetratricopeptide Repeat Region Is the Functional Domain of SSN6, a Nuclear Phosphoprotein of Saccharomyces cerevisiae. Janet Schultz, Linda Marshall-Carlson, and Marian Carlson...... 4744 4756 Two Systems of Glucose Repression of the GAL] Promoter in Saccharomyces cerevisiae. Jeffrey S. Flick and Mark Johnston ...... 4757-4769 A Single Amino Acid Change in CUP2 Alters Its Mode of DNA Binding. Carla Buchman, Petra Skroch, Wendy Dixon, Thomas D. Tullius, and Michael Karin ...... 4778-4787 Overlapping Reading Frames at the LYSS Locus in the Yeast Yarrowia lipolytica. Jian-Wu Xuan, Philippe Fournier, Nathalie Declerck, Marion Chasles, and Claude Gaillardin ...... 4795-4806 Enhancers for RNA Polymerase I in Mouse Ribosomal DNA. Craig S. Pikaard, Louise K. Pape, Sheryl L. Henderson, Kenneth Ryan, Mark H. Paalman, Margaret A. Lopata, Ronald H. Reeder, and Barbara Sollner-Webb ...... 4816-4825 Brain and Muscle Creatine Kinase Genes Contain Common Tar-Rich Recognition Protein-Binding Regulatory Elements. Robert A. Horlick, Grace M. Hobson, James H. Patterson, Mark T. Mitchell, and Pamela A. Benfield 4826-4836 Induction of the Granulocyte-Macrophage Colony-Stimulating Factor (CSF) Re- ceptor by Granulocyte CSF Increases the Differentiative Options of a Murine Hematopoietic Progenitor Cell. Brent L. Kreider, Paul D. Phillips, Michael B. Prystowsky, Neelam Shirsat, Jacalyn H. Pierce, Robert Tushinski, and Giovanni Rovera...... 4846-4853 A Nonerythroid GATA-Binding Protein Is Required for Function of the Human Preproendothelin-1 Promoter in Endothelial Cells. David B. Wilson, David M. Dorfman, and Stuart H. Orkin ...... 4854 4862 Sequences within an Upstream Activation Site in the Yeast Enolase Gene EN02 Modulate Repression of EN02 Expression in Strains Carrying a Null Muta- tion in the Positive Regulatory Gene GCRI. Janice P. Holland, Paul K. Brindle, and Michael J. Holland ...... 4863-4871 Multiple Factors Bind the Upstream Activation Sites of the Yeast Enolase Genes ENO] and EN02: ABFI Protein, like Repressor Activator Protein RAP1, Binds cis-Acting Sequences Which Modulate Repression or Activation of Transcription. Paul K. Brindle, Janice P. Holland, Catherine E. Willett, Michael A. Innis, and Michael J. Holland ...... 4872-4885 Induction of a Chicken Small Heat Shock (Stress) Protein: Evidence of Multilevel Posttranscriptional Regulation. Brent V. Edington and Lawrence E. Hightower ...... 4886-4898

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An Initiation Zone of Chromosomal DNA Replication Located Upstream of the c-myc Gene in Proliferating HeLa Cells. Lyubomir Vassilev and Edward M. Johnson ...... 4899-4904 Upstream Elements Repress Premature Expression of an Aspergillus Developmen- tal Regulatory Gene. Thomas H. Adams and William E. Timberlake...... 4912-4919 The First Pre-rRNA-Processing Event Occurs in a Large Complex: Analysis by Gel Retardation, Sedimentation, and UV Cross-Linking. Susan Kass and Barbara Sollner-Webb...... 4920-4931 Point Mutations in the Yeast Histone H4 Gene Prevent Silencing of the Silent Mating Type Locus HML. Eun-Chung Park and Jack W. Szostak...... 4932-4934 SPT6, an Essential Gene That Affects Transcription in Saccharomyces cerevisiae, Encodes a Nuclear Protein with an Extremely Acidic Amino Terminus. Michele S. Swanson, Marian Carlson, and Fred Winston...... 4935-4941 Sequence Requirements in Different Steps of the Pre-mRNA Splicing Reaction: Analysis by the RNA Modification-Exclusion Technique. Katharine M. Lang and Walter Keller...... 4942-4947 Highly Repeated Sites in the Apolipoprotein(a) Gene Recognized by Methylated DNA-Binding Protein, a Sequence-Specific DNA-Binding Protein. Kenneth C. Ehrlich and Melanie Ehrlich...... 4957-4960 Domains of Human c-myc Protein Required for Autosuppression and Cooperation with ras Oncogenes Are Overlapping. Linda J. Z. Penn, Mary W. Brooks, Edward M. Laufer, Trevor D. Littlewood, Jay P. Morgenstern, Gerrard I. Evan, William M. F. Lee, and Hartmut Land...... 4961-4966 Oxidant Stress Leads to Transcriptional Activation of the Human Heme Oxygen- ase Gene in Cultured Skin Fibroblasts. Stephen M. Keyse, Lee Ann Applegate, Yvonne Tromvoukis, and Rex M. Tyrrell ...... 4967-4969 The Mouse Ribosomal DNA Promoter Has More-Stringent Requirements In Vivo than In Vitro. Sheryl L. Henderson and Barbara Sollner-Webb ...... 4970-4973 A Cellular Factor Binds to the Herpes Simplex Virus Type 1 Transactivator Vmw65 and Is Required for Vmw65-Dependent Protein-DNA Complex As- sembly with Oct-1. Peter Xaio and J. P. Capone...... 4974-4977 Transcriptional Regulation of the Transforming Growth Factor ,1 Promoter by v-src Gene Products Is Mediated through the AP-1 Complex. Maria C. Birchenall-Roberts, Francis W. Ruscetti, James Kasper, Hy-De Lee, Rosalind Friedman, Andrew Geiser, Michael B. Sporn, Anita B. Roberts, and Seong-Jin Kim...... 4978-4983 A Short, Highly Repetitive Element in Intron -1 of the Human c-Ha-ras Gene Acts as a Block to Transcriptional Readthrough by a Viral Promoter. Noel F. Lowndes, Pierre Bushel, Laurel Mendelsohn, Jing Wu, Muh-Yong Yen, and Maggi Allan ...... 4990-4995 Minimal Subenhancer Requirements for High-Level Polyomavirus DNA Replica- tion: A Cell-Specific Synergy of PEA3 and PEAl Sites. Rosemary Rochford, Christie T. Davis, Kenneth K. Yoshimoto, and Luis P. Villarreal...... 4996-5001 Repression of the a-Fetoprotein Gene Promoter by Progesterone and Chimeric Receptors in the Presencr of Hormones and Antihormones. Bernard Turcotte, Marc-Etienne Meyer, Marie-Therese Bocquel, Luc Belanger, and Pierre Chambon ...... 5002-5006

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trans-Activation of Human Alcohol Dehydrogenase Gene Expression in Hepatoma Cells by C/EBP Molecules Bound in a Novel Arrangement Just 5' and 3' to the TATA Box. Mark J. Stewart, Mary Lou Shean, and Gregg Duester ... 5007-5010 Functional State of the Epidermal Growth Factor-Receptor Complexes during Their Internalization in A-431 Cells. Alexander Nesterov, Galina Reshetnikova, Nina Vinogradova, and Nikolay Nikolsky...... 5011-5014 Nerve Growth Factor (NGF) Induces Neuronal Differentiation in Neuroblastoma Cells Transfected With the NGF Receptor cDNA. Hiroshi Matsushima and Emil Bogenmann ...... 5015-5020 The Testis-Specific Transcript (ferl) of the Tyrosine Kinase FER Is Expressed during Spermatogenesis in a Stage-Specific Manner. Eli Keshet, Ahuva Itin, Kineret Fischman, and Uri Nir ...... 5021-5025

CELL GROWTH AND DEVELOPMENT G Protein Mutations That Alter the Pheromone Response in Saccharomyces cere- visiae. David E. Stone and Steven I. Reed...... 4439-4446 Recombinant Transforming Growth Factor Type P3: Biological Activities and Receptor-Binding Properties in Isolated Bone Cells. Peter ten Dijke, Kenneth K. Iwata, Colin Goddard, Christian Pieler, Ernesto Canalis, Thomas L. McCarthy, and Michael Centrella ...... 4473 4479 Glucose-Induced Hyperaccumulation of Cyclic AMP and Defective Glucose Re- pression in Yeast Strains with Reduced Activity of Cyclic AMP-Dependent Protein Kinase. Kaishusha Mbonyi, Linda Van Aelst, Juan Carlos Arguelles, Arnold W. H. Jans, and Johan M. Thevelein...... 4518-4523 Overexpression of Protein Kinase C in HT29 Colon Cancer Cells Causes Growth Inhibition and Tumor Suppression. Paul M. Choi, Kam-Meng Tchou-Wong, and I. Bernard Weinstein ...... 4650-4657 Differential Repair of UV Damage in rad Mutants of Saccharomyces cerevisiae: A Possible Function of G2 Arrest upon UV Irradiation. Carrol Terleth, Paul Schenk, Raymond Poot, Jaap Brouwer, and Pieter van de Putte ...... 4678-4684 Diverse Forms of a Receptor for Acidic and Basic Fibroblast Growth Factors. Daniel E. Johnson, Pauline L. Lee, John Lu, and Lewis T. Williams ...... 4728-4736 Characterization and cDNA Cloning of Phospholipase C-,y, a Major Substrate for Heparin-Binding Growth Factor 1 (Acidic Fibroblast Growth Factor)-Acti- vated Tyrosine Kinase. Wilson H. Burgess, Craig A. Dionne, June Kaplow, Robert Mudd, Robert Friesel, Asher Zilberstein, Joseph Schlessinger, and Michael Jaye...... 4770-4777 Characterization of a Novel Promoter Insertion in the c-rel Locus. Neil Kabrun, Nat Bumstead, Michael J. Hayman, and Paula J. Enrietto...... 4788-4794 K28, A Unique Double-Stranded RNA Killer Virus of Saccharomyces cerevisiae. Manfred J. Schmitt and Donald J. Tipper...... 4807-4815 Effect of Myogenic and Adipogenic Differentiation on Expression of Colony-Stim- ulating Factor Genes. Maureen A. Harrington, J. H. Frederik Falkenburg, Raymond Daub, and Hal E. Broxmeyer...... 4948-4952 The Genes for Leukemia Inhibitory Factor and Interleukin-6 Are Expressed in Mouse Blastocysts prior to the Onset of Hemopoiesis. Richard Murray, Frank Lee, and Choy-Pik Chiu...... 4953-4956

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CELL AND ORGANELLE STRUCTURE AND ASSEMBLY Characterization of a cDNA Encoding a Cysteine-Rich Cell Surface Protein Lo- cated in the Flagellar Pocket of the Protozoan Trypanosoma brucei. Mary Gwo-Shu Lee, Bernard E. Bihain, David G. Russell, Richard J. Deckelbaum, and Lex H. T. Van der Ploeg...... 4506-4517 A Drosophila Ribosomal Protein Functions in Mammalian Cells. C. G. Maki, D. D. Rhoads, J.-J. Diaz, and D. J. Roufa...... 4524-4528 Characterization of an In Vitro Assay for Import of 3-Phosphoglycerate Kinase into the Glycosomes of Trypanosoma brucei. Jurg M. Sommer, Julia A. Thissen, Marilyn Parsons, and Ching C. Wang...... 4545-4554 Cell Surface Processing of Transforming Growth Factor a Precursor by Elastases in Arterioles. Susan G. Mueller, Andrew J. Paterson, and Jeffrey E. Kudlow ...... 4596-4602 The Saccharomyces cerevisiae DPMI Gene Encoding Dolichol-Phosphate-Mannose Synthase Is Able To Complement a Glycosylation-Defective Mammalian Cell Line. P. J. Beck, P. Orlean, C. Albright, P. W. Robbins, M.-J. Gething, and J. F. Sambrook ...... 4612-4622 Characterization of Yeast Vps33p, a Protein Required for Vacuolar Protein Sort- ing and Vacuole Biogenesis. Lois M. Banta, Thomas A. Vida, Paul K. Herman, and Scott D. Emr ...... 4638-4649 Overexpression of a Leaderless Form of Yeast Cytochrome c Oxidase Subunit Va Circumvents the Requirement for a Leader Peptide in Mitochondrial Im- port. Lori K. Dircks and Robert 0. Poyton...... 4984-4986

CHROMOSOME STRUCTURE AND DYNAMICS Homologous Recombination in Hybridoma Cells: Dependence on Time and Frag- ment Length. Marc J. Shulman, Lynette Nissen, and Catherine Collins 4466-4472 Isolation of the URAS Gene from Cryptococcus neoformans var. neoformans and Its Use as a Selective Marker for Transformation. Jeffrey C. Edman and K. J. Kwon-Chung ...... 4538-4544 Expression of the Yeast PHRI Gene Is Induced by DNA-Damaging Agents. Joseph Sebastian, Betsy Kraus, and Gwendolyn B. Sancar...... 4630-4637 Mapping an Origin of DNA Replication at a Single-Copy Locus in Exponentialy Proliferating Mammalian Cells. Lyubomir T. Vassilev, William C. Burhans, and Melvin L. DePamphilis ...... 4685-4689 Transcription Stimulates Homologous Recombination in Mammalian Cells. Jac A. Nickoloff and Richard J. Reynolds ...... 4837-4845 A Conserved Sequence in Histone H2A Which Is a Ubiquitination Site in Higher Eucaryotes Is Not Required for Growth in Saccharomyces cerevisiae. Paul S. Swerdlow, Tillman Schuster, and Dan Finley...... 4905-4911 Use of an HpaII-Polymerase Chain Reaction Assay To Study DNA Methylation in the Pgk-l CpG Island of Mouse Embryos at the Time of X-Chromosome In- activation. Judith Singer-Sam, Mark Grant, Jeanne M. LeBon, Kazuhiko Okuyama, Verne Chapman, Marilyn Monk, and Arthur D. Riggs...... 4987-4989

Date of Issue: 21 August 1990