Efficient Professional Protein and Antibody Platforms ANAPC1 Antibody Basic information: Catalog No.: UMA21402 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal 7G9B3 Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: The antibody was purified by immunogen affinity chromatography. Useful Information: WB:1:500 - 1:2000 ICC:1:100 - 1:500 Applications: FCM:1:200 - 1:400 ELISA:1:10000 Reactivity: Human Specificity: This antibody recognizes ANAPC1 protein. Purified recombinant fragment of human ANAPC1 (AA: 12-155) expressed in Immunogen: E. Coli. This encodes a subunit of the -promoting complex. This complex is an E3 that regulates progression through the Description: metaphase to anaphase portion of the by ubiquitinating proteins which targets them for degradation. Uniprot: Q9H1A4 BiowMW: 216kDa Buffer: Purified antibody in PBS with 0.05% sodium azide Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Figure 2:Western blot analysis using ANAPC1 mAb against human ANAPC1 (AA: 12-155) re- combinant protein. (Expected MW is 41.9 kDa)

Figure 3:Western blot analysis using ANAPC1 mAb against HEK293 (1) and ANAPC1 (AA: 12-155)-hIgGFc transfected HEK293 (2) cell ly- sate.

Figure 4:Immunofluorescence analysis of Hela cells using ANAPC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phal- loidin. Secondary antibody from Fisher (Cat#: 35503)

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]