SAN TA C RUZ BI OTEC HNOL OG Y, INC . NBPF1 shRNA Plasmid (h): sc-106281-SH

BACKGROUND STORAGE AND RESUSPENSION NBPF1 (neuroblastoma breakpoint family, member 1), also known as AD2, Store lyophilized shRNA plasmid DNA at 4° C with desiccant. Stable for NBG, AB13, AB14 or AB23, is a 1,214 amino acid protein that localizes to at least one year from the date of shipment. Once resuspended, store at the cytoplasm and contains eight NBPF domains. Expressed in a variety of 4° C for short term storage or -80° C for long term storage. Avoid repeated tissues, NBPF1 belongs to the neuroblastoma breakpoint family and is encoded freeze thaw cycles. by a which maps to 1. spans 260 Resuspend lyophilized shRNA plasmid DNA in 200 µl of the deionized water million base pairs, contains over 3,000 and comprises nearly 8% of provided. Resuspension of the shRNA plasmid DNA in 200 µl of deionized the . Chromosome 1 houses a large number of disease-asso - water makes a 0.1 µg/µl solution in a 10 mM Tris, 1 mM EDTA buffered ciated genes, including those that are involved in familial adenomatous poly - solu tion. posis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of APPLICATIONS cancers, including head and neck cancer, malignant melanoma and multiple myeloma. NBPF1 shRNA Plasmid (h) is recommended for the inhibition of NBPF1 expression in human cells. REFERENCES SUPPORT REAGENTS 1. Weise, A., Starke, H., Mrasek, K., Claussen, U. and Liehr, T. 2005. New insights into the evolution of chromosome 1. Cytogenet. Genome Res. For optimal shRNA Plasmid transfection efficiency, Santa Cruz Biotechnology’s 108: 217-222. shRNA Plasmid Transfection Reagent: sc-108061 (0.2 ml) and shRNA Plasmid Transfection Medium: sc-108062 (20 ml) are recommended. Control shRNAs 2. Vandepoele, K., Van Roy, N., Staes, K., Speleman, F. and van Roy, F. 2005. are available as 20 µg lyophilized plasmid DNA. Each encodes a scrambled A novel gene family NBPF: intricate structure generated by gene duplica - shRNA sequence that will not lead to the specific degradation of any known tions during primate evolution. Mol. Biol. Evol. 22: 2265-2274. cel lular mRNA. Control shRNA Plasmids include: sc-108060, sc-108065 and 3. Marzin, Y., Jamet, D., Douet-Guilbert, N., Morel, F., Le Bris, M.J., Morice, P., sc- 108066. Abgrall, J.F., Berthou, C. and De Braekeleer, M. 2006. Chromosome 1 abnor - ma lities in multiple myeloma. Anticancer Res. 26: 953-959. RT-PCR REAGENTS 4. Online Mendelian Inheritance in Man, OMIM™. 2007. Johns Hopkins Semi-quantitative RT-PCR may be performed to monitor NBPF1 gene expres - University, Baltimore, MD. MIM Number: 610501. World Wide Web URL: sion knockdown using RT-PCR Primer: NBPF1 (h)-PR: sc-106281-PR (20 µl). http://www.ncbi.nlm.nih.gov/omim/ Annealing temperature for the primers should be 55-60° C and the extension temperature should be 68-72° C. 5. Vandepoele, K., Andries, V., Van Roy, N., Staes, K., Vandesompele, J., Laureys, G., De Smet, E., Berx, G., Speleman, F. and van Roy, F. 2008. A RESEARCH USE constitutional translocation t(1;17 )(p36.2;q11.2) in a neuroblastoma patient disrupts the human NBPF1 and ACCN1 genes. PLoS ONE 3: e2207. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product and all replicates and derivatives 6. Vandepoele, K., Andries, V. and van Roy, F. 2009. The NBPF1 promoter has for research purposes conducted by the buyer in his laboratory only (whether been recruited from the unrelated EVI5 gene before simian radiation. Mol. the buyer is an academic or for-profit entity). The buyer cannot sell or other - Biol. Evol. 26: 1321-1332. wise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party, or otherwise use this product CHROMOSOMAL LOCATION or its components or materials made using this product or its components Genetic : NBPF1 (human) mapping to 1p36.13. for Commercial Purposes.

PRODUCT NBPF1 shRNA Plasmid (h) is a target-specific lentiviral vector plasmid encod - ing a 19-25 nt (plus hairpin) shRNA designed to knock down gene expression. Each plasmid contains a puromycin resistance gene for the selection of cells stably expressing shRNA. Each vial contains 20 µg of lyophilized shRNA plas mid DNA. Suitable for up to 20 transfections. Also see NBPF1 siRNA (h): sc-106281 and NBPF1 shRNA (h) Lentiviral Particles: sc-106281-V as alternate gene silencing products.

PROTOCOLS See our web site at www.scbt.com for detailed protocols and support products.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com