DOCSLIB.ORG

Cytogenetic Evolution of Genus Salmo

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Cytogenetic Evolution of Genus Salmo Cytogenetic Evolution of Genus Salmo by Song Lin M.Sc., University of New Brunswick, 2012 B.Sc., Dalhousie University, 2009 B.Sc., Fujian Agriculture and Forestry University, 2009 Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in the Department of Molecular Biology and Biochemistry Faculty of Science © Song Lin 2019 SIMON FRASER UNIVERSITY Spring 2019 Copyright in this work rests with the author. Please ensure that any reproduction or re-use is done in accordance with the relevant national copyright legislation. Approval Name: Song Lin Degree: Doctor of Philosophy Title: Cytogenetic Evolution of Genus Salmo Examining Committee: Chair: Tim Audas Assistant Professor William Davidson Senior Supervisor Professor Nancy Hawkins Supervisor Associate Professor Nicholas Harden Supervisor Professor Ben Koop Internal Examiner Professor Biology University of Victoria Kerry Naish External Examiner Professor School of Aquatic and Fishery Sciences University of Washington Date Defended/Approved: February 21, 2019 ii Abstract The ancestral genome duplication that occurred around 80-100 MYA in the common ancestor of extant salmonids induced a stressed auto-tetraploid genome. This was followed by expansion of transposable elements which is believed to facilitate large- scale chromosomal rearrangements. The species radiation did not occur until around 40- 50 million years later when earth’s climate was going into a cooling stage. As the result of the large-scale genome reorganization, present-day salmonids share large syntenic collinear blocks in a species-specific order. In the present study, our first aim was to visualize these rearrangements by taking advantage of genus Salmo (including two sole members, namely, Atlantic salmon (S. salar) and brown trout (S. trutta)) where species not only exhibit distinct karyotypes, but also intra-species chromosomal polymorphisms. Moreover, another aim of present study was to provide cytogenetic support for confirming the identity of sex determining gene, sdY, in Atlantic salmon by revealing its physical locations in both European and North American derived Tasmanian Atlantic salmon. In the genus of Salmo, large scale rearrangements including fusions, translocations, fissions and possible chromosome arm loss were evident in the present study, giving rise to the extant karyotypes of S. salar and S. trutta. The fact that no polymorphic karyotype of the S. trutta was observed in our study and other publications suggests a relative stable genomic background. In contrast, polymorphic karyotypes due to Robertsonian rearrangements of three pairs of chromosomes were found in Canadian subpopulations of S. salar. The fixation of the homozygous translocation in most Canadian populations indicates possible fixation of advantageous mutations and suggests a mild to near-neutral underdominance of this rearrangement in its ancestral heterozygous state. On the other hand, the observation of potentially deleterious Robertsonian tandem fusions in all Canadian populations indicate the initial rearrangement likely took place in a small effective population with subsequent spreading into other river populations through colonization. In our effort to visualize the physical locations of sdY, a single-copy gene, the gene was indeed found residing on the sub-telocentric region of Ssa02q in European Atlantic salmon, consistent with results from previous SEX mapping studies. In comparison, multiple locations including Ssa02q, Ssa03p and Ssa06p were shown to be locations of sdY in a North American derived Tasmanian population, supporting a previous study suggesting a jumping gene theory. iii Keywords: sex determination; sex differentiation; salmonids; cytogenetics iv Acknowledgements To my beloved wife Xiating Huang and my lovely daughter Juliet Lin: because I owe it all to you. Many Thanks! I am grateful to my father and mother Jiantong Lin and Jianmei Kong, who have provided me through moral and emotional support in my life. I am also grateful to my other family members and friends who have supported me along the way. A very special gratitude and respect goes out to my senior supervisor, Dr. William S. Davidson for his patient guidance and tremendous trust and support. I sincerely wish him a forever happy retirement life. I am also grateful to my supervisor committee members: Dr. Nancy Hawkins and Dr. Nicholas Harden. Nick not only taught me the fundamental knowledge related to molecular biology as the instructor for MBB331, but also provides valuable ideas and supports along the way. Nancy has been providing brilliant ideas and guidance whenever it is needed. I truly grateful to all their valuable assistance throughout the PhD research. And finally, last but by no means least, also to all members of Davidson Lab: Evelyn, Alejandra, Will, Michelle, Rico … it was great sharing laboratory with all of you. Thanks for all your encouragement! v Table of Contents Approval .......................................................................................................................... ii Abstract .......................................................................................................................... iii Acknowledgements ......................................................................................................... v Table of Contents ........................................................................................................... vi List of Tables ................................................................................................................. viii List of Figures................................................................................................................. ix Chapter 1. Introduction .............................................................................................. 1 1.1. Whole genome duplication and how it relates to the evolution of Salmonidae ....... 1 1.2. The cytogenetic evolution in Salmonidae ............................................................... 6 1.3. Sex determination in animals ............................................................................... 10 1.3.1. Sex determination in mammals .................................................................... 11 1.3.2. Sex determination in Drosophila .................................................................. 13 1.3.3. Sex determination in Caenorhabditis elegans .............................................. 14 1.3.4. Sex determination in fish ............................................................................. 15 1.4. The importance of conducting molecular cytogenetic research in the genus Salmo and general procedures used in fluorescence in-situ hybridization (FISH) ..................... 19 1.5. Objectives of this study ........................................................................................ 21 1.6. References .......................................................................................................... 22 Chapter 2. Cytogenetic variation among populations of Canadian Atlantic salmon (Salmo salar)......................................................................................... 35 2.1. Abstract ............................................................................................................... 36 2.2. Introduction .......................................................................................................... 37 2.3. Material and methods .......................................................................................... 39 2.3.1. Blood collection and lymphocyte culture ...................................................... 39 2.3.2. BAC probe preparation and FISH ................................................................ 40 2.3.3. Genotyping with mtDNA and nuclear DNA markers ..................................... 42 2.4. Results ................................................................................................................ 42 2.5. Discussion ........................................................................................................... 50 2.6. Reference ............................................................................................................ 53 Chapter 3. Cytogenetic relationship between members of genus Salmo. ........... 56 3.1. Abstract ............................................................................................................... 57 3.2. Introduction .......................................................................................................... 58 3.3. Material and methods .......................................................................................... 60 3.3.1. Fish blood collection .................................................................................... 60 3.3.2. C-banding techniques .................................................................................. 61 3.3.3. BAC marker assignment and fluorescence in-situ hybridization ................... 61 3.4. Results ................................................................................................................ 63 3.5. Discussion ........................................................................................................... 72 3.6. References .......................................................................................................... 75 vi Chapter 4. Turnover of sex chromosomes in Tasmanian Atlantic salmon (Salmo. salar) ..……………………………………………………………………………………78
Load more

Recommended publications
  • Reproduction De L'huître Perlière Pinctada Margaritifera
    UNIVERSITÉ DE LA POLYNÉSIE FRANÇAISE École doctorale du Pacifique (ED469) Ifremer- UMR 241 Ecosystèmes Insulaires Océaniens THÉSE DE DOCTORAT Présentée par Vaihiti TEANINIURAITEMOANA Pour l’obtention du grade de : Docteur de l’Université de la Polynésie française Discipline : Biologie des populations et écologie Spécialité : Génomique des organismes marins Reproduction de l’huître perlière Pinctada margaritifera : Étude des déterminants du sexe femelle chez l’adulte Soutenue le 8 Décembre 2014 à l’Université de la Polynésie française MEMBRES DU JURY ~ Anne-Sophie MARTINEZ, Maître de Conférences, Université de Caen Basse-Normandie Rapporteur Yann GUIGUEN, Directeur de Recherche, INRA Rapporteur Jean-Claude GAERTNER, Directeur de Recherche, IRD Examinateur Yannick GUEGUEN, Cadre de Recherche, Ifremer Examinateur Gilles LE MOULLAC, Cadre de Recherche, Ifremer Co-Directeur Nabila GAERTNER-MAZOUNI, Professeur, Université de la Polynésie française Co-Directrice Avant-propos Avant-propos Cette thèse a été réalisée au sein de l’École Doctorale du Pacifique (ED469) dans l’Unité Mixte de Recherche Ecosystèmes Insulaires Océaniens (UMR 241 EIO) sous la co-direction du Professeur Nabila GAERTNER-MAZOUNI (UMR 241 EIO, Université de la Polynésie française) et du Docteur Gilles LE MOULLAC (UMR 241 EIO, Ifremer Tahiti); et sous l’encadrement scientifique du Docteur Arnaud HUVET (UMR 6539 LEMAR, Ifremer Brest). Cette thèse a bénéficié d’un co-financement de la part de l’Institut français de recherche pour l’exploitation de la mer (Ifremer) et de la part de la Délégation à la Recherche de Polynésie française. À l’Ifremer, ces travaux de recherche s’intègrent au sein du projet PJ0707 « Développement durable de la perliculture » et ont été financés d’une part, par l’ANR-AGROBIOSPHERE dans le cadre du programme de recherche Polyperl (Gestion intégrée et adaptation de la perliculture en Polynésie française dans le contexte du changement global, 2012-2015) et d’autre part, par le contrat de projet État-Pays Biodiperl (Préservation de la biodiversité des stocks d’huîtres perlières P.
    [Show full text]
  • Genome Sequence of the Euryhaline Javafish Medaka, Oryzias Javanicus
    GENOME REPORT Genome Sequence of the Euryhaline Javafish Medaka, Oryzias javanicus: A Small Aquarium Fish Model for Studies on Adaptation to Salinity Yusuke Takehana,* Margot Zahm,† Cédric Cabau,‡ Christophe Klopp,†,‡ Céline Roques,§ Olivier Bouchez,§ Cécile Donnadieu,§ Celia Barrachina,** Laurent Journot,** Mari Kawaguchi,†† Shigeki Yasumasu,†† Satoshi Ansai,‡‡ Kiyoshi Naruse,‡‡ Koji Inoue,§§ Chuya Shinzato,§§ Manfred Schartl,***,†††,‡‡‡ Yann Guiguen,§§§,1 and Amaury Herpin§§§,****,1,2 *Department of Animal Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bioscience and Technology, 1266 Tamura, Nagahama 526-0829, Japan, †Plate-forme bio-informatique Genotoul, Mathématiques et Informatique Appliquées de Toulouse, INRAE, Castanet Tolosan, France, ‡SIGENAE, GenPhySE, Université de Toulouse, INRAE, § ENVT, Castanet Tolosan, France, INRAE, US 1426, GeT-PlaGe, Genotoul, Castanet-Tolosan, France, **MGX, Biocampus Montpellier, CNRS, INSERM, University of Montpellier, Montpellier, France, ††Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioi-cho Chiyoda-ku, Tokyo 102-8554, Japan, ‡‡Laboratory of Bioresources, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji-cho, Okazaki 444-8585, §§ Japan, Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa 277-8564, Japan, ***University of Wuerzburg, Developmental Biochemistry, Biocenter, 97074 Wuerzburg, Germany, †††Comprehensive Cancer Center Mainfranken, University Hospital, 97080 Wuerzburg,
    [Show full text]
  • Khan Msc 19 Aug 2017 Sex Determination and Sex Differentiation Genes in Lampreys AK MD AK Aug 26
    Investigation of candidate sex determination and sex differentiation genes in sea lamprey, Petromyzon marinus, and Pacific lamprey, Entosphenus tridentatus By Arfa A. Khan A thesis submitted to the Faculty of Graduate Studies of The University of Manitoba In partial fulfillment of the requirements of the degree of MASTER OF SCIENCE Department of Biological Sciences University of Manitoba Winnipeg, Manitoba Copyright © 2017 by Arfa Khan i Abstract Genes associated with sex differentiation (the process by which undifferentiated gonads develop into testes or ovaries) are relatively conserved among vertebrates, but those associated with sex determination (the genetic and/or environmental “switch” that activates differentiation) are more variable, particularly in fishes. Virtually nothing is known regarding the genetic basis of these processes in lampreys, one of the two extant groups of jawless fishes. Therefore, I tested whether 19 candidate sex determination genes identified from other vertebrates showed sex specific sequence differences in sea lamprey, Petromyzon marinus, and Pacific lamprey, Entosphenus tridentatus; 11 and seven genes amplified in sea and Pacific lampreys, respectively, but none showed sex specific differences. I also used qRT-PCR to measure expression of seven candidate sex differentiation genes (e.g., SOX9, DMRT1, WT1, DAZAP1) prior to and during testicular differentiation in sea lamprey, and found that expression patterns were consistent with their presumed role in other vertebrates. ii Acknowledgements I would like to thank my advisor, Dr. Margaret Docker, for all her support. I couldn’t have done it without her mentoring and passion for learning more about the mystery that is lampreys. You have been influential in my growth as a student during my time at the University of Manitoba and have motivated me every step of the way.
    [Show full text]
  • The Genetics of Sexual Development in East African Cichlid Fishes
    The Genetics of Sexual Development in East African Cichlid Fishes Inauguraldissertation Zur Erlangung der Würde eines Doktors der Philosophie vorgelegt der Philosophisch-Naturwissenschaftlichen Fakultät der Universität Basel von Corina Heule aus Altstätten, Schweiz Basel, 2015 Genehmigt von der Philosophisch-Naturwissenschaftlichen Fakultät auf Antrag von Prof. Dr. Walter Salzburger (Universität Basel) und Prof. Judith Mank (University College London) Basel, den 12.11.2013 Prof. Dr. Jörg Schibler (Dekan, Universität Basel) Contents Introduction ..................................................................1 Chapter 1 .................................................................... 11 The ontogenetic development of egg-spots in the haplochromine cichlid fish Astatotilapia burtoni Chapter 2 .................................................................... 21 Genetics of sexual development – an evolutionary playground for fish Chapter 3 .................................................................... 37 Expression and sequence evolution of aromatase cyp19a1 and other sexual development genes in East African cichlid fishes Chapter 4 .................................................................... 59 Genetics and timing of sex determination in the East African cichlid fish Astatotilapia burtoni Perspectives ............................................................... 79 Acknowledgments ...................................................... 85 Introduction The cichlid fishes of East Africa Cichlid fishes occur in tropical
    [Show full text]
  • Sex Determination in Annual Fishes: Searching for the Master Sex-Determining Gene in Austrolebias Charrua (Cyprinodontiformes, Rivulidae)
    Genetics and Molecular Biology, 37, 2, 364-374 (2014) Copyright © 2014, Sociedade Brasileira de Genética. Printed in Brazil www.sbg.org.br Research Article Sex determination in annual fishes: Searching for the master sex-determining gene in Austrolebias charrua (Cyprinodontiformes, Rivulidae) María José Arezo1, Nicolás Papa1, Verónica Guttierrez2, Graciela García2 and Nibia Berois1 1Sección Biología Celular, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. 2Sección Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. Abstract Evolution of sex determination and differentiation in fishes involves a broad range of sex strategies (herma- phroditism, gonochorism, unisexuality, environmental and genetic sex determination). Annual fishes inhabit tempo- rary ponds that dry out during the dry season when adults die. The embryos exhibit an atypical developmental pattern and remain buried in the bottom mud until the next rainy season. To elucidate genomic factors involved in the sex determination in annual fish, we explored the presence of a candidate sex-specific gene related to the cascade network in Austrolebias charrua. All phylogenetic analyses showed a high posterior probability of occurrence for a clade integrated by nuclear sequences (aprox. 900 bp) from both adults (male and female), with partial cDNA frag- ments of A. charrua from juveniles (male) and the dsx D. melanogaster gene. The expressed fragment was detected from blastula to adulthood stages showing a sexually dimorphic expression pattern. The isolated cDNA sequence is clearly related to dsx D. melanogaster gene and might be located near the top of the sex determination cascade in this species. Keywords: annual fish, development, sex determination, doublesex gene related sequence.
    [Show full text]
  • The Genomic and Genetic Toolbox of the Teleost Medaka (Oryzias Latipes)
    GENETICS | REVIEW GENETIC TOOLBOX The Genomic and Genetic Toolbox of the Teleost Medaka (Oryzias latipes) Stephan Kirchmaier,* Kiyoshi Naruse,† Joachim Wittbrodt,*,1 and Felix Loosli‡,1 *Centre for Organismal Studies, University of Heidelberg, INF 230, 69120 Heidelberg, Germany, †Laboratory of Bioresources, National Institute for Basic Biology, Okazaki 444-8585, Aichi, Japan, and ‡Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, Germany ABSTRACT The Japanese medaka, Oryzias latipes, is a vertebrate teleost model with a long history of genetic research. A number of unique features and established resources distinguish medaka from other vertebrate model systems. A large number of laboratory strains from different locations are available. Due to a high tolerance to inbreeding, many highly inbred strains have been established, thus providing a rich resource for genetic studies. Furthermore, closely related species native to different habitats in Southeast Asia permit comparative evolutionary studies. The transparency of embryos, larvae, and juveniles allows a detailed in vivo analysis of development. New tools to study diverse aspects of medaka biology are constantly being generated. Thus, medaka has become an important vertebrate model organism to study development, behavior, and physiology. In this review, we provide a comprehensive overview of established genetic and molecular-genetic tools that render medaka fish a full-fledged vertebrate system. KEYWORDS teleost; Oryzias latipes; medaka; genetics; genomics EDAKA (Oryzias latipes) is a small freshwater fish of embryo (Sampetrean et al. 2009). Thus, in the laboratory, Mthe family Adrianichthyidae in the order Beloni- temperature can be used to control the developmental speed formes. It is closely related to other members of the super- without adverse effects.
    [Show full text]
  • Characterization of Sex Determination and Sex Differentiation Genes in Latimeria
    Characterization of Sex Determination and Sex Differentiation Genes in Latimeria Mariko Forconi1, Adriana Canapa1*, Marco Barucca1, Maria A. Biscotti1, Teresa Capriglione2, Francesco Buonocore3, Anna M. Fausto3, Daisy M. Makapedua4, Alberto Pallavicini5, Marco Gerdol5, Gianluca De Moro5, Giuseppe Scapigliati3, Ettore Olmo1., Manfred Schartl6. 1 Dipartimento di Scienze della Vita e dell’Ambiente, Universita` Politecnica delle Marche, Ancona, Italy, 2 Dipartimento di Biologia Strutturale e Funzionale, Universita` Federico II, Napoli, Italy, 3 Dipartimento per l’Innovazione nei Sistemi Biologici, Agroalimentari e Forestali, Universita` della Tuscia, Viterbo, Italy, 4 Faculty of Fisheries and Marine Science, University of Sam Ratulangi, Manado, Indonesia, 5 Dipartimento di Scienze della Vita, Universita` di Trieste, Trieste, Italy, 6 Physiological Chemistry, Biocenter, University of Wuerzburg, Wuerzburg, Germany Abstract Genes involved in sex determination and differentiation have been identified in mice, humans, chickens, reptiles, amphibians and teleost fishes. However, little is known of their functional conservation, and it is unclear whether there is a common set of genes shared by all vertebrates. Coelacanths, basal Sarcopterygians and unique ‘‘living fossils’’, could help establish an inventory of the ancestral genes involved in these important developmental processes and provide insights into their components. In this study 33 genes from the genome of Latimeria chalumnae and from the liver and testis transcriptomes of Latimeria menadoensis, implicated in sex determination and differentiation, were identified and characterized and their expression levels measured. Interesting findings were obtained for GSDF, previously identified only in teleosts and now characterized for the first time in the sarcopterygian lineage; FGF9, which is not found in teleosts; and DMRT1, whose expression in adult gonads has recently been related to maintenance of sexual identity.
    [Show full text]
  • Sex Determination in the Teleost Medaka, Oryzias
    A N RV260- G E39-14 ARI 15 O ctober 2005 12:55 Sex D etermination in the Teleost Medaka, Oryzias latipes Masaru Matsuda PR ES T O , Japan Science and Technology Corporation (JS T ), Laboratory of Reproductive Biology, N ational Institute for Basic Biology, O kazaki 444-8585, Japan; email: [email protected] Annu. Rev. G enet. K ey Words 2005. 39:293–307 sexual differentiation of gonads, sex-determining gene, medaka, T he Annual Review of Genetics is online at D MY D MRT1, Oryzias species http://genet.annualreviews.org Abstract doi: 10.1146/ annurev.genet.39.110304.095800 Although the sex of most animals is determined by genetic informa- Copyright c 2005 by tion, sex-determining genes had been identified only in mammals, by AUSTRALIAN NATIONAL UNIVERSITY on 03/22/09. For personal use only. Annu. Rev. Genet. 2005.39:293-307. Downloaded from arjournals.annualreviews.org Annual Reviews. All rights several flies, and the worm Caenorhabditis elegans until the recent reserved discovery of D MY (D M-domain gene on the Y chromosome) in 0066-4197/05/1215- the sex-determining region on the Y chromosome of the teleost 0293$20.00 fish medaka, Oryzias latipes. Functional and expression analyses of D MY have shown it to be the master gene for male sex determi- nation in the medaka. T he only sex-determining genes found so far in vertebrates are Sry and D MY. T herefore, the medaka is ex- pected to become a good experimental animal for investigating the precise mechanisms involved in primary sex determination in non- mammalian vertebrates.
    [Show full text]
  • Vertebrate Sex-Determining Genes Play Musical Chairs
    C. R. Biologies 339 (2016) 258–262 Contents lists available at ScienceDirect Comptes Rendus Biologies w ww.sciencedirect.com Trajectories of genetics, 150 years after Mendel/Trajectoires de la ge´ne´tique, 150 ans apre`s Mendel Vertebrate sex-determining genes play musical chairs Les ge`nes qui de´terminent le sexe des verte´bre´s jouent aux chaises musicales a a,1 a,b a Qiaowei Pan , Jennifer Anderson , Sylvain Bertho , Amaury Herpin , c c b,d,e Catherine Wilson , John H. Postlethwait , Manfred Schartl , a, Yann Guiguen * a Inra, Fish Physiology and Genomics Laboratory, 35042 Rennes, France b University of Wuerzburg, Physiological Chemistry, Biocenter, 97074 Wu¨rzburg, Germany c University of Oregon, Institute of Neuroscience, Eugene, OR 97403, USA d Comprehensive Cancer Center Mainfranken, University Hospital, 97080 Wu¨rzburg, Germany e Texas Institute for Advanced Study and Department of Biology, Texas A&M University, College Station, Texas 77843, USA A R T I C L E I N F O A B S T R A C T Article history: Sexual reproduction is one of the most highly conserved processes in evolution. However, Received 18 March 2016 the genetic and cellular mechanisms making the decision of whether the undifferentiated Accepted after revision 26 April 2016 gonad of animal embryos develops either towards male or female are manifold and quite Available online 10 June 2016 diverse. In vertebrates, sex-determining mechanisms range from environmental to simple or complex genetic mechanisms and different mechanisms have evolved repeatedly and Keywords: independently. In species with simple genetic sex-determination, master sex-determining Sex-determination genes lying on sex chromosomes drive the gonadal differentiation process by switching on Evolution a developmental program, which ultimately leads to testicular or ovarian differentiation.
    [Show full text]
  • Genome Sequence of the Euryhaline Javafish Medaka, Oryzias Javanicus: a Small Aquarium 2 Fish Model for Studies on Adaptation to Salinity
    bioRxiv preprint doi: https://doi.org/10.1101/593673; this version posted March 29, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Genome sequence of the euryhaline Javafish medaka, Oryzias javanicus: a small aquarium 2 fish model for studies on adaptation to salinity. 3 4 Yusuke Takehana1, Margot Zahm2, Cédric Cabau3, Christophe Klopp2, 3, Céline Roques4, Olivier 5 Bouchez4, Cécile Donnadieu4, Celia Barrachina5, Laurent Journot5, Mari Kawaguchi6, Shigeki 6 Yasumasu6, Satoshi Ansai7, Kiyoshi Naruse7, Koji Inoue8, Chuya Shinzato8, Manfred Schartl9, 10, 11, 7 Yann Guiguen12, * and Amaury Herpin12, *, ¶. 8 9 10 11 12 AFFILIATIONS 13 1 Department of Animal Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bioscience and 14 Technology, 1266 Tamura, Nagahama 526-0829, Japan. 15 2 Plate-forme bio-informatique Genotoul, Mathématiques et Informatique Appliquées de 16 Toulouse, INRA, Castanet Tolosan, France. 17 3 SIGENAE, GenPhySE, Université de Toulouse, INRA, ENVT, Castanet Tolosan, France. 18 4 INRA, US 1426, GeT-PlaGe, Genotoul, Castanet-Tolosan, France 19 5 MGX, Biocampus Montpellier, CNRS, INSERM, University of Montpellier, Montpellier, France. 20 6 Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia 21 University, 7-1 Kioi-cho Chiyoda-ku, Tokyo 102-8554, Japan. 22 7 Laboratory of Bioresources, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji-cho, 23 Okazaki 444-8585, Japan. 24 8 Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, 25 Kashiwa 277-8564, Japan. 26 9 University of Wuerzburg, Developmental Biochemistry, Biocenter, 97074 Wuerzburg, Germany.
    [Show full text]
  • Researchonline@JCU
    ResearchOnline@JCU This file is part of the following work: Budd, Alyssa (2020) Epigenetic effects of temperature on sex change in barramundi, Lates calcarifer. PhD Thesis, James Cook University. Access to this file is available from: https://doi.org/10.25903/95mj%2Dpk34 © 2020 Alyssa Budd. The author has certified to JCU that they have made a reasonable effort to gain permission and acknowledge the owners of any third party copyright material included in this document. If you believe that this is not the case, please email [email protected] Epigenetic effects of temperature on sex change in barramundi, Lates calcarifer Thesis submitted by Alyssa Budd 26 May 2020 For the degree of Doctor of Philosophy In Agriculture, Environmental and Related Studies College of Science and Engineering James Cook University ACKNOWLEDGEMENTS Thank you. To the funding bodies and collaborating partners that enabled the work to be carried out. To Professor Dean Jerry, for showing me how you ‘blaze your own trail’ and demonstrating a stubborn sense of optimism that is incredibly powerful. I have learnt a lot from you, I hope you have learnt from me too. To Dr Jose Domingos, for your unwavering enthusiasm and genuine interest in my results. To Dr Roger Huerlimann, for your generosity in offering your time, extensive knowledge and friendship. To Dr Carmel McDougall and Dr Nick Wade, for your unfailing kindness and mentorship. To Dr Carolyn-Smith Keune, for your tremendous dedication to the functionality of the MEEL lab and well-being of its members. To Associate Professor Jan Strugnell, for the opportunity to work for you and your patience whilst I wrote up.
    [Show full text]
  • 467 Pelvic-Fin Brooding in a New Species of Riverine
    THE RAFFLES BULLETIN OF ZOOLOGY 2012 THE RAFFLES BULLETIN OF ZOOLOGY 2012 60(2): 467–476 Date of Publication: 31 Aug.2012 © National University of Singapore PELVIC-FIN BROODING IN A NEW SPECIES OF RIVERINE RICEFISH (ATHERINOMORPHA: BELONIFORMES: ADRIANICHTHYIDAE) FROM TANA TORAJA, CENTRAL SULAWESI, INDONESIA Fabian Herder Zoologisches Forschungsmuseum Alexander Koenig, Sektion Ichthyologie, Adenauerallee 160, D-53113 Bonn, Germany Email: [email protected] Renny Kurnia Hadiaty Ichthyology Laboratory, Division of Zoology, Research Center for Biology, Indonesian Institute of Sciences (LIPI) JL. Raya Bogor Km 46, Cibinong 16911, Indonesia Email: [email protected] Arne W. Nolte Max-Planck-Institut fuer Evolutionsbiologie, Abteilung Evolutionsgenetik, August-Thienemannstrasse 2, 24306 Ploen Email: [email protected] ABSTRACT. — A new species of ricefi sh is described from a hill stream in Tana Toraja, Sulawesi. Oryzias eversi, new species, is distinguished from all other adrianichthyids in Sulawesi by having a low number of fi n rays in anal (17–18 (19)) and dorsal (10–12) fi ns, only 33–36 scales in lateral midline, ½14 transverse scale rows at dorsal fi n origin, 30–32 (33) vertebrae, small eyes (28.2–35.5% of head length), a conspicuous blackish male courtship colouration, and pelvic brooding behaviour similar to lacustrine ricefi shes. Female Oryzias eversi carry the eggs until the embryos hatch, and show a conspicuous abdominal concavity and extended pelvic fi ns, accommodating and holding the clutch of eggs. The eggs are connected to the female for the whole time of development by attaching fi laments that protrude from the female’s urogenital pore.
    [Show full text]