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ACADL Rabbit pAb

Catalog No.: A1266 1 Publications

Basic Information Background

Catalog No. The encoded by this belongs to the acyl-CoA dehydrogenase family, which A1266 is a family of mitochondrial flavoenzymes involved in and branched chain amino-acid . This protein is one of the four that catalyze the initial Observed MW step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are 47kDa the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia. Calculated MW 47kDa

Category Primary antibody

Applications WB, IHC, IF

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:500 - 1:2000 Gene ID Swiss Prot 33 P28330 IHC 1:50 - 1:200 Immunogen 1:50 - 1:200 IF Recombinant fusion protein containing a sequence corresponding to amino acids 31-210 of human ACADL (NP_001599.1).

Synonyms ACADL;ACAD4;LCAD

Contact Product Information

www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification

Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of various cell lines, using ACADL antibody (A1266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded rat ovary using ACADL antibody embedded human lung cancer using embedded mouse heart using ACADL (A1266) at dilution of 1:100 (40x lens). ACADL antibody (A1266) at dilution of antibody (A1266) at dilution of 1:100 (40x 1:100 (40x lens). lens).

Immunofluorescence analysis of HeLa cells using ACADL antibody (A1266) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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