cells Article The Bioinformatic and In Vitro Studies of Clostridioides Difficile Aminopeptidase M24 Revealed the Immunoreactive KKGIK Peptide Katarzyna Pacyga 1 , Agnieszka Razim 1,2,* , Gayane Martirosian 3 , Małgorzata Aptekorz 3 , Andrzej Szuba 4,5, Andrzej Gamian 2, Andrzej Myc 2,6 and Sabina Górska 1,* 1 Department of Microbiology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland;
[email protected] 2 Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland;
[email protected] (A.G.);
[email protected] (A.M.) 3 Department of Medical Microbiology, School of Medical Science in Katowice, Medical University of Silesia, 40-752 Katowice, Poland;
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[email protected] (M.A.) 4 Division of Angiology, Wroclaw Medical University, 51-618 Wroclaw, Poland;
[email protected] 5 Department of Internal Medicine, 4th Military Hospital in Wroclaw, 50-981 Wroclaw, Poland 6 MNIMBS, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109-5648, USA * Correspondence:
[email protected] (A.R.);
[email protected] (S.G.); Tel.: +48-71-3371-172 (ext. 183) (A.R.); +48-71-3371-172 (ext. 148) (S.G.) Received: 17 February 2020; Accepted: 5 May 2020; Published: 7 May 2020 Abstract: Clostridioides difficile (CD) is a Gram-positive pathogen responsible for CD-associated disease (CDAD), which is characterized by symptoms ranging from mild diarrhea to pseudomembranous colitis. This work is an attempt to respond to the need of novel methods for CD infection (CDI) prevention, since the number of CDI cases is still rising.