Wignall-Fleming, Elizabeth Bowie (2019) Investigations into the dynamics of paramyxovirus infections by high-throughput sequencing. PhD thesis. https://theses.gla.ac.uk/40905/ Copyright and moral rights for this work are retained by the author A copy can be downloaded for personal non-commercial research or study, without prior permission or charge This work cannot be reproduced or quoted extensively from without first obtaining permission in writing from the author The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the author When referring to this work, full bibliographic details including the author, title, awarding institution and date of the thesis must be given Enlighten: Theses https://theses.gla.ac.uk/
[email protected] Investigations into the dynamics of paramyxovirus infections by high-throughput sequencing Elizabeth Bowie Wignall-Fleming University of Glasgow A thesis submitted for the degree of Doctor of Philosophy College of Medical, Veterinary & Life Sciences University of Glasgow July 2018 Abstract The paramyxovirus family can cause a broad spectrum of diseases from mild febrile illnesses to more severe diseases that may require hospitalisation and can in the most serious cases have fatal outcomes. Understanding the virus infection dynamics is fundamental to the development of novel targets for therapeutic and vaccine development. The advancement of High-throughput sequencing (HTS) has revolutionised biomedical research providing unparalleled opportunities to answer complex questions. In this study we developed a workflow using directional analysis of HTS data to gain a unique opportunity to simultaneously analyse the kinetics of virus transcription and replication for PIV5 strain W3, PIV2, MuV and PIV3.