ILF3 Antibody Cat. No.: 29-328

ILF3 Antibody

Antibody used in IHC on Human Skeletal muscle at Antibody used in WB on Human Raji at 0.0625 ug/ml. 4.0-8.0 ug/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human ILF3.

TESTED APPLICATIONS: ELISA, IHC, WB

ILF3 antibody can be used for detection of ILF3 by ELISA at 1:62500. ILF3 antibody can be APPLICATIONS: used for detection of ILF3 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1207 - Raji Cell Lysate

PREDICTED MOLECULAR 76 kDa, 95 kDa, 75 kDa WEIGHT:

October 2, 2021 1 https://www.prosci-inc.com/ilf3-antibody-29-328.html Properties

PURIFICATION: Antibody is purified by peptide affinity chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store ILF3 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: ILF3

ILF3, DRBF, DRBP76, MMP4, MPHOSPH4, MPP4, NF-AT-90, NF90, NFAR, NFAR-1, TCP80, ALTERNATE NAMES: CBTF, NF110, NF90a, NF90b, NFAR2, NF110b, TCP110

ACCESSION NO.: NP_004507

PROTEIN GI NO.: 24234753

GENE ID: 3609

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

October 2, 2021 2 https://www.prosci-inc.com/ilf3-antibody-29-328.html ILF3 may facilitate double-stranded RNA-regulated expression at the level of post- transcription. ILF3 can act as a translation inhibitory which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 can regulate protein arginine N-methyltransferase 1 activity. ILF3 may regulate transcription of the IL2 gene during T-cell activation. It can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.Nuclear factor of activated T-cells (NFAT) is a required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is BACKGROUND: a heterodimer of 45 kDa and 90 kDa , the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.

REFERENCES: 1) Nie, Y., (2005) Mol. Cell. Biol. 25 (16), 6956-6963.

ANTIBODIES FOR RESEARCH USE ONLY.

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