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Docontrol WEB Documents DIATABS™ User’s Guide Diagnostic Tablets for Bacterial Identification Latest version DIATABS™- DIAGNOSTIC TABLETS for BACTERIAL IDENTIFICATION The DIATABS™, Diagnostic Tablets (D.T.), developed by Rosco are identification tests made available as individual tablets, which allow the microbiologist a free choice of the most appropriate tests for identification. Most of the Diatabs™ are rapid tests (chromogenic enzymatic reactions, and modified conventional tests). The tablets may be used as single tests to show isolated microbial properties or as part of cost-effective systems of their own. This User's Guide describes more than 80 different types of tests for identification of the clinically important groups of bacteria, and has been written by J.B. Casals on behalf of Rosco Diagnostica. The 8th Ed. 2009 of the DIATABS™ User's Guide contains updated text, tables and references, all necessary information when using Diatabs tablets for identification of bacteria and yeasts. Finally, we would like to quote The Manual of Clinical Microbiology 8th Ed. 2003, page 893: “Individually available tablets … (Rosco Diagnostic Tablets …) are much cheaper than commercial kits, they can be applied in a number of situations and allow flexibility in tailoring the set to best suit special needs”. The User's Guide is available at our website www.rosco.dk and updated information is continuously included. ROSCO Diagnostica A/S is welcoming any feedback and questions on bacterial identification from users directly ([email protected]) or through our representatives. ROSCO – Taastrupgaardsvej 30 – 2630 Taastrup – Denmark – Tel. +45 43 99 33 77 – www.rosco.dk – [email protected] Side 2 af 147 Table of Contents ACETAMIDE HYDROLYSIS (ACM) .......................................................................................................................................................... 5 ALKALINE PHOSPHATASE (Alk P) .......................................................................................................................................................... 6 ALPHA GLUCOSIDASE (-GLU) BETA-GLUCOSIDASE (ß-GLU) ............................................................................................................... 8 Alphabetic INDEX of Abbreviations and Codes .................................................................................................................................. 10 ALPHA- FUCOSIDASE (-FUC) ............................................................................................................................................................. 13 ALPHA-GALACTOSIDASE (-GAL) ....................................................................................................................................................... 17 AMINOPEPTIDASES ............................................................................................................................................................................ 18 ARGININE AMINOPEPTIDASE (ARG) ................................................................................................................................................... 19 Anaerobes, Presumptive Identification with Oxgall (bile), Brilliant Green and Antibiotic Tablets ..................................................... 20 ARGININE DIHYDROLASE (ADH) ......................................................................................................................................................... 22 BACITRACIN 40 UNITS (BACIT) Neo-Sensitabs .................................................................................................................................... 24 BACITRACIN LOW (BaL) ...................................................................................................................................................................... 25 Bacterial identification using Diatabs ................................................................................................................................................. 27 BETA LACTAMASE (Acido) .................................................................................................................................................................. 31 BETA-FUCOSIDASE (ß-FUC) ................................................................................................................................................................. 32 BETA-GLUCURONIDASE (PGUA) ......................................................................................................................................................... 34 Beta-lactamases (ESBL, AmpC, MBL) detection using Neo-Sensitabs and Diatabs ............................................................................ 35 BETA-N-ACETYLGLUCOSAMINIDASE (ß-NAG) ..................................................................................................................................... 39 BETA-XYLOSIDASE (ß-XYL) .................................................................................................................................................................. 42 CITRATE (CIT) ...................................................................................................................................................................................... 44 CYCLOHEXIMIDE (CYC) ....................................................................................................................................................................... 45 DEFEROXAMINE (DEFRX) .................................................................................................................................................................... 46 C-390 .................................................................................................................................................................................................. 49 S.P.S. (SPS) .......................................................................................................................................................................................... 50 DOUBLE TEST Diatabs ......................................................................................................................................................................... 52 ESCULIN HYDROLYSIS (ESC) BILE ESCULIN (BE) ................................................................................................................................... 53 FOSFOMYCIN 70 µg (FOSFO) Neo-Sensitabs ...................................................................................................................................... 56 FACTOR X, V, and X+V ........................................................................................................................................................................ 58 FURAZOLIDONE 50 µg (FURAZ) MUPIROCIN 10 µg (MUPIR) Neo-Sensitabs ...................................................................................... 60 GAMMA-GLUTAMYL AMINOPEPTIDASE (γ-GLU)................................................................................................................................ 62 GENTAMICIN 250 µg (GN250), KANAMYCIN 500 µg (KA500), STREPTOMYCIN 500 µg (ST500) Neo-Sensitabs ................................. 65 GLYCOSIDASES.................................................................................................................................................................................... 67 HIPPURATE HYDROLYSIS (HIP) ............................................................................................................................................................ 69 INDOXYL ACETATE (IAC) ..................................................................................................................................................................... 73 LDC / INDOLE (LDC/IND) ..................................................................................................................................................................... 76 LEUCINE AMINOPEPTIDASE (LAP) ...................................................................................................................................................... 78 LYSINE DECARBOXYLASE (LDC) ORNITHINE DECARBOXYLASE (ODC) ................................................................................................. 81 METRONIDAZOLE 5 µg (MTR.5) .......................................................................................................................................................... 83 METRONIDAZOLE 50 µg (MTR50) ....................................................................................................................................................... 85 NITRATE REDUCTION (NO3) ................................................................................................................................................................ 86 NOVOBIOCIN 5 µg (NOVO5) Neo-Sensitabs ....................................................................................................................................... 88 O/129 (Vibriostaticum) (O/129) ......................................................................................................................................................... 90 ODC / INDOLE (ODC/IND) ................................................................................................................................................................... 92 ONPG - Beta-Galactosidase (ONPG) ..................................................................................................................................................
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