Enzyme-Linked ImmunoSpot (ELISpot)

Enzyme-Linked ImmunoSpot assay (ELISpot) is designed for the quantitation of single secreting cell detection among 1,000,000 cells in a 96 well plate. The high sensitivity of ELISpot made it a power- ful technique in detecting low frequency -secreting cells (about 1 in 300,000 cells) and is widely used in cancer research, disease studies and vaccine development.

Stimulant Cell Biotin Anti-cytokine A spot represent single responding cell

Cytokine Release

Streptavidin- Remove enzyme cells conjugate PVDF membrane Cytokine-specific capture antibody

ELISpot v.s. Conventional ELISA

Comparison ELISpot Conventional ELISA Enzyme-Linked ImmunoSpot Assay (ELISpot) Sample Format Cell Culture Supernatant

Measurement Format Single Cell Supernatant

T cell Functional Study Yes No

Cytokine Per Cell Net Cytokine Per Cell Cytokine Productivity (Source of Cytokine) (Source Non-distinguishable)

Resolution High Subtle Differences Non-detectable

Dual Detection Yes No

Storage Time Weeks to Months Hours

Detection Device ELISpot Reader/Scanner ELISA Reader (camera with visual analysis software) (photometer)

Cytokine Related Research Tools

● Pre-designed Chimeras for RNAi ● Antibody Pairs ● Recombinant Proteins ● ELISA Kits

Abnova ● MaxPab ● Antibody Arrays ● Monoclonal Antibodies ● Mammalian Cell Lysates

● Polyclonal Antibodies ● DNA FISH Probe

Abnova Corporation 9F, No. 108, Jhouzih St., Neihu, Taipei 114, Taiwan Tel: + 886 2 8751 1888 Fax: + 886 2 6602 1218 www.abnova.com ELISpot Kits

Catalog No. Product Name Research Area

KA3938 IL8 (Human) ELISpot Kit Angiogenesis KA3913 IL1A (Human) ELISpot Kit Apoptosis KA3914 IL2RA (Human) ELISpot Kit Apoptosis KA3939 CCL2 (Human) ELISpot Kit Apoptosis KA3915 IL17A (Human) ELISpot Kit Apoptosis KA3919 FASLG (Human) ELISpot Kit Apoptosis KA3920 GZMB (Human) ELISpot Kit Apoptosis KA3921 IL1B (Human) ELISpot Kit Apoptosis KA3933 PRF1 (Human) ELISpot Kit Apoptosis KA3931 IL12 (Human) ELISpot Kit Binding KA3914 IL2RA (Human) ELISpot Kit CD Markers KA3919 FASLG (Human) ELISpot Kit CD Markers KA3922 IL2 (Human) ELISpot Kit Cell Ad/Junc/Cytoskel KA3934 Il2 (Mouse) ELISpot Kit Cell Ad/Junc/Cytoskel KA3940 CSF1 (Human) ELISpot Kit Cell Cycle KA3924 IL5 (Human) ELISpot Kit Cell Cycle KA3928 IL6 (Human) ELISpot Kit Cell Cycle KA3912 CSF2 (Human) ELISpot Kit Cytokine KA3916 IL17F (Human) ELISpot Kit Cytokine KA3917 IL-17A/F (Human) ELISpot Kit Cytokine KA3918 IFNG (Human) ELISpot Kit Cytokine KA3929 IL10 (Human) ELISpot Kit Cytokine KA3935 Ifng (Mouse) ELISpot Kit Cytokine KA3936 Ifng (Rat) ELISpot Kit Cytokine KA3923 IL4 (Human) ELISpot Kit Plasma/Serum KA3930 TNF (Human) ELISpot Kit Plasma/Serum KA3932 IL13 (Human) ELISpot Kit Plasma/Serum KA3937 Tnf (Rat) ELISpot Kit Plasma/Serum

Abnova Corporation 9F, No. 108, Jhouzih St., Neihu, Taipei 114, Taiwan Tel: + 886 2 8751 1888 Fax: + 886 2 6602 1218 www.abnova.com