Monoclonal Antibody to SNAP25 - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AM08404PU-N Monoclonal Antibody to SNAP25 - Purified

Alternate names: RIC4, SNAP, SNAP-25, SUP, Super Protein, Synaptosomal-associated 25 kDa protein, Synaptosomal-associated protein 25, ric-4 Quantity: 50 µg Concentration: 1 mg/ml Background: SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre- synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. SNAP25 is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems and it associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9 amino acids in the central portion of the protein. Uniprot ID: P60880 NCBI: NP_003072.2 GeneID: 6616 Host / Isotype: Mouse / IgG1 Recommended Isotype SM10P (for use in human samples), SM20P (for use in rat samples), AM03095PU-N Controls: Clone: 3H2886 Immunogen: Protein extract. Crude Human synaptic immunoprecipitate. Format: State: Liquid purified Ig fraction Purification: Protein A Chromatography Buffer System: PBS, pH 7.2 containing 40% Glycerol and 0.09% Sodium Azide as preservative. Applications: ELISA. Immunohistochemistry on Paraffin Sections: 5 µg/ml. Western Blot: 1/250 - 1/2500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: Appears to recognize the Human pre-synaptic protein, SNAP-25; an antigen of 26-27kD molecular weight. It will recognize SNAP-25 fusion protein from COS cells, but not with the fusion protein from bacterial systems. Research studies have used this antibody to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 1 / 2 AM08404PU-N: Monoclonal Antibody to SNAP25 - Purified

Species Reactivity: Tested: Human. Expected from sequence similarity: Hamster, Porcine and Rat. Add. Information: Predicted molecular weight: 29 kDa. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Pictures: AM08404PU-N SNAP25 antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 2 / 2