First State Record of the Northern Two-lined Salamander in Michigan
February 20, 2009
Nicole Soderberg Kimberly Balash Teresa Yoder Ernest Szuch Overview
Murphy Lake State Game Area (MLSGA) Unique herpetofauna of MLSGA Northern Two-lined Salamander natural history results of 2008 field season Molecular genetics Murphy Lake State Game Area
Tuscola county East of Millington
Murphy Lake State Game Area (MLSGA)
Millington
Created by Teresa Yoder
Created by Teresa Yoder MLSGA
10.7 km2 of habitat islands surrounded by agriculture Hemlock-Northern Hardwoods community MLSGA
Topography influenced by glaciers end-moraine ridges pitted outwash plains kettle ponds Unique Herpetofauna of MLSGA
3 species in MI known only to occur in MLSGA Six-lined Racerunner (Aspidoscelis sexlineata) Northern Dusky Salamander (Desmognathus fuscus) Northern Two-lined Salamander (Eurycea bislineata) Unique Herpetofauna of MLSGA
Photo taken by James Harding Six-lined Racerunner (Aspidoscelis sexlineata)
Photo taken by James Harding
Photo taken by Teresa Yoder Unique Herpetofauna of MLSGA
Northern Dusky Salamander (Desmognathus fuscus)
Carlson (Yoder) and Szuch (2005) Unique Herpetofauna of MLSGA
Northern Two-lined Salamander (Eurycea bislineata)
Soderberg, Szuch, and Yoder (In Press) Northern Two-lined Salamander (Eurycea bislineata)
Identification Northern Two-lined Salamander (Eurycea bislineata)
Distribution
Conant and Collins 1998 Northern Two-lined Salamander (Eurycea bislineata)
Adult habitat Northern Two-lined Salamander (Eurycea bislineata)
Adult habitat Northern Two-lined Salamander (Eurycea bislineata)
Breeding
Photo taken by Teresa Yoder Northern Two-lined Salamander (Eurycea bislineata)
Breeding habitat Northern Two-lined Salamander (Eurycea bislineata)
Breeding habitat Northern Two-lined Salamander (Eurycea bislineata)
Nest Northern Two-lined Salamander (Eurycea bislineata)
Nest-guarding Northern Two-lined Salamander (Eurycea bislineata)
Larvae
Photo taken by James Harding Northern Two-lined Salamander (Eurycea bislineata)
Larvae habitat Northern Two-lined Salamander (Eurycea bislineata)
Voucher Northern Two-lined Salamander (Eurycea bislineata)
Voucher voucher number: UMMZ 239001
Results of 2008 field season (April – October)
April 12 Gravid female Photo by Teresa by Yoder Photo
Nest May 24
Nest-guarding female
July Larvae Photo by James by Harding Photo
July 4 Voucher
~ 16 adults TOTAL > 20 larvae Introduced or Native?
Six-lined Racerunner recent research points to native
Conant and Collins 1998 Introduced or Native?
Six-lined Racerunner recent research points to native
Northern Dusky Salamander?
Conant and Collins 1998 Introduced or Native?
Six-lined Racerunner recent research points to native
Northern Dusky Salamander?
Northern Two-lined Salamander?
Conant and Collins 1998 Molecular Genetics
Tissue collection Molecular Genetics
Extract DNA PCR to amplify mitochondrial ND2 gene Sequence ND2 gene Phylogenetic analysis Current and Future Research
Natural history Stream surveys Fluorescent powder tracking Molecular work Introduced or native? treat as native unless evidence shows otherwise
Acknowledgements
I thank Dr. Ann Sturtevant for providing guidance and support
I thank Matthew Chatfield , Nickole Hatley and Kara Pattison for their help and advice
I thank James Harding of MSU for identification of the original Eurycea bislineata individual
I thank Gregory Schneider of UMMZ for identification and deposition of the Eurycea bislineata voucher
Approval of protocol #10098 was given by UCUCA
Land use permits to use state property for this research were issued by Cass City office of DNR Questions?