Plant Bioinformatics METHODS IN MOLECULAR BIOLOGYTM John M. Walker, SERIES EDITOR 419. Post-Transcriptional Gene Regulation, edited by 390. Protein Targeting Protocols, Second Edition, edited Jeffrey Wilusz, 2008 by Mark van der Giezen, 2007 418. Avidin–Biotin Interactions: Methods and 389. Pichia Protocols, Second Edition, edited by James Applications, edited by Robert J. McMahon, 2008 M. Cregg, 2007 417. Tissue Engineering, Second Edition, edited by 388. Baculovirus and Insect Cell Expression Protocols, Hannsjörg Hauser and Martin Fussenegger, 2007 Second Edition, edited by David W. 416. Gene Essentiality: Protocols and Bioinformatics, Murhammer, 2007 edited by Andrei L. Osterman, 2008 387. Serial Analysis of Gene Expression (SAGE): Digital 415. Innate Immunity, edited by Jonathan Ewbank and Gene Expression Profiling, edited by Kare Lehmann Eric Vivier, 2007 Nielsen, 2007 414. Apoptosis in Cancer: Methods and Protocols, edited 386. 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MRSA Protocols, edited by Yinduo Ji, 2007 Moorhead, 2007 METHODS IN MOLECULAR BIOLOGYTM Plant Bioinformatics Methods and Protocols Edited by David Edwards Australian Centre for Plant Functional Genomics, Institute for Molecular Biosciences and School of Land, Crop and Food Sciences, University of Queensland, Brisbane, Australia ©2007 Humana Press Inc. 999 Riverview Drive, Suite 208 Totowa, New Jersey 07512 www.humanapress.com All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise without written permission from the Publisher. Methods in Molecular BiologyTM is a trademark of The Humana Press Inc. All papers, comments, opinions, conclusions, or recommendations are those of the author(s), and do not necessarily reflect the views of the publisher. This publication is printed on acid-free paper. 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The fee code for users of the Transactional Reporting Service is: [978-1-58829-653-5/07 $30]. Printed in the United States of America. 10987654321 Library of Congress Control Number: 2007933141. Preface Bioinformatics is a new and rapidly evolving field of research that is being driven by the development and application of various omics technologies. The term bioinformatics means different things to different people, and following the theme of this series, this volume focuses on applied bioinformatics with specific applications to crops and model plants. This volume is aimed at plant biologists who have an interest in or requirement for accessing the huge amount of data being generated by high-throughput technologies. The volume would also be of interest to bioinformaticians and computer scientists who would benefit from an introduction to the different tools and systems available for plant research. Bioinformatics was initially driven by the need to understand and exploit the information being generated by gene and genome sequencing projects, the scope of bioinformatics now extends from the genome to the phenome. It is the integration of information relating to heritable agronomic traits, including important metabolic profiles, with the emerging genome and transcriptome data that will drive plant research and bioinformatics developments into the future. One observation during the production of this volume is the increasing integration of both related data from different plants and also the integration of multiple diverse forms of data. I expect this trend to continue as this field of research continues to develop in the future. Scientific research by its nature progresses and changes, and this is especially true for bioinformatics. The rapid evolution of the tools and systems described in this volume may change during the lifetime of this edition, and it is suggested that the reader consults the relevant web pages directly as they frequently host detailed list of updates and changes. David Edwards Contents Preface................................................................... v Contributors .............................................................. xi 1. The EMBL Nucleotide Sequence and Genome Reviews Databases Peter Sterk, Tamara Kulikova, Paul Kersey, and Rolf Apweiler ..... 1 2. Using GenBank David Wheeler ................................................... 23 3. A Collection of Plant-Specific Genomic Data and Resources at NCBI Tatiana Tatusova, Brian Smith-White, and James Ostell ........... 61 4. UniProtKB/Swiss-Prot Emmanuel Boutet, Damien Lieberherr, Michael Tognolli, Michel Schneider, and Amos Bairoch .................................. 89 5. Plant Database Resources at The Institute for Genomic Research Agnes P. Chan, Pablo D. Rabinowicz, John Quackenbush, C. Robin Buell, and Chris D. Town ............................. 113 6. MIPS Plant Genome Information Resources Manuel Spannagl, Georg Haberer, Rebecca Ernst, Heiko Schoof, and Klaus F. X. Mayer.......................................... 137 7. HarvEST Timothy J. Close, Steve Wanamaker, Mikeal L. Roose,