Datasheet Version: 1.0.0 Revision date: 03 Dec 2020

SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1) Antibody Catalogue No.:abx004102

Western blot analysis of extracts of various cell lines, using SMARCE1 antibody (abx004102) at 1/1000 dilution.

Immunohistochemistry of paraffin-embedded human gastric cancer using SMARCE1 Antibody (abx004102) at dilution of 1/200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using SMARCE1 Antibody (abx004102) at dilution of 1/200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using SMARCE1 Antibody (abx004102) at dilution of 1/200 (40x lens). For Reference Only

Immunohistochemistry of paraffin-embedded human colon using SMARCE1 Antibody (abx004102) at dilution of 1/100 (40x lens).

v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 1 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected] Datasheet Version: 1.0.0 Revision date: 03 Dec 2020

Immunohistochemistry of paraffin-embedded human esophageal cancer using SMARCE1 Antibody (abx004102) at dilution of 1/100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using SMARCE1 Antibody (abx004102) at dilution of 1/100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using SMARCE1 Antibody (abx004102) at dilution of 1/100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using SMARCE1 Antibody (abx004102) at dilution of 1/200 (40x lens).

For Reference Only Immunohistochemistry of paraffin-embedded human esophageal cancer using SMARCE1 Antibody (abx004102) at dilution of 1/200 (40x lens).

v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 2 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected] Datasheet Version: 1.0.0 Revision date: 03 Dec 2020

Immunohistochemistry of paraffin-embedded human gastric cancer using SMARCE1 Antibody (abx004102) at dilution of 1/200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using SMARCE1 Antibody (abx004102) at dilution of 1/200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse stomach using SMARCE1 Antibody (abx004102) at dilution of 1/200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse cancer using SMARCE1 Antibody (abx004102) at dilution of 1/200 (40x lens).

For Reference Only Immunofluorescence analysis of U2OS cells using SMARCE1 antibody (abx004102). Blue: DAPI for nuclear staining.

SMARCE1 Antibody is a Rabbit Polyclonal antibody against SMARCE1. The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA,

v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 3 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected] Datasheet Version: 1.0.0 Revision date: 03 Dec 2020

which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Target: SMARCE1

Clonality: Polyclonal

Reactivity: Human, Mouse

Tested Applications: WB, IHC, IF/ICC

Host: Rabbit

Recommended dilutions: WB: 1/500 - 1/2000, IHC: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.

Conjugation: Unconjugated

Immunogen: Recombinant protein of human SMARCE1.

Isotype: IgG

Form: Liquid

Purification: Affinity purified.

Storage: Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

UniProt Primary AC: Q969G3 (UniProt, ExPASy)

Gene Symbol: SMARCE1 GeneID: For6605 Reference Only KEGG: hsa:6605

String: 9606.ENSP00000323967

Molecular Weight: Calculated MW: 34 kDa, 37 kDa, 39 kDa, 41 kDa, 42 kDa, 46 kDa Observed MW: 55 kDa

Buffer: PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.

Concentration: > 1 mg/ml

v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 4 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected] Datasheet Version: 1.0.0 Revision date: 03 Dec 2020

Note: This product is for research use only.

For Reference Only

v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 5 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected]