DOCSLIB.ORG

Expression of Albumin Messenger RNA Detected by in Situ Hybridization in Preneoplastic and Neoplastic Lesions in Rat Liver1

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Expression of Albumin Messenger RNA Detected by in Situ Hybridization in Preneoplastic and Neoplastic Lesions in Rat Liver1 (CANCER RESEARCH 46, 5903-5912, November 1986] Expression of Albumin Messenger RNA Detected by in Situ Hybridization in Preneoplastic and Neoplastic Lesions in Rat Liver1 Michael Schwarz,2 Gabriele Peres, David G. Beer, MaimónMaor, Albrecht Buchmann, Werner Kunz, and Henry C. Pitot German Cancer Research Center, Institut of Biochemistry, Im Neuenheimer Feld 280, D-6900 Heidelberg, Federal Republic of Germany [M. S., G. P., M. M., A. B., W. K.J, and McArdle Laboratory for Cancer Research, The Medical School, University of Wisconsin, Madison, Wisconsin 53706 ¡D.G. B., H. C. P.] ABSTRACT synthesis and excretion are frequently reduced in chemically induced primary hepatoma and transplantable hepatoma cells The process of chemical hepatocarcinogenesis is characterized by the in vivo and in vitro (2, 4, 7-10). For reasons which are not fully appearance of preneoplastic lesions showing changes in the expression of various marker enzymes. We have analyzed the phenotype of small understood the expression of albumin was found to vary exten preneoplastic foci and expansively growing nodules in liver sections sively in hepatoma and primary hepatocellular carcinoma (7). obtained from rats treated with various carcinogens. Changes within the We were therefore interested to know whether such variability lesions in canalicular adenosine triphosphatase, •y-glutamyltranspepti- in albumin expression could already be detected in early pre dase, NADPH-(cytochrome P-450) reducíase,cytochrome P-450 PB2, neoplastic and neoplastic lesions in rat liver. These focally epoxide hydrolase, and glycogen content were detected by means of growing lesions, which are generally regarded as tumor precur enzyme histochemical and immunohistochemical staining procedures. In sors, are characterized by changes in the expression of marker parallel sections the expression of albumin messenger RNA was investi enzymes such as canalicular ATPase, GGT, glucose-6-phospha- gated by in situ hybridization using a "S-labeled albumin specific com tase, NADPH-(cytochrome P-450) reducíase, EH, and micro- plementary DNA probe. In general, small preneoplastic lesions showed somal monooxygenases (11-20). Both the multiplicity of en unchanged levels of albumin messenger RNA. In contrast, the expression of albumin messenger RNA was found to be reduced to varying degrees zyme changes which occur in distinct patterns within individual in large hepatic nodules. An expression of a-fetoprotein messenger RNA foci and nodules and the inducibility of certain enzymes point could not be detected in any of the nodules. No direct correlation between towards alterations in processes directly linked to cell differ the enzyme phenotype of the lesions and the degree in reduction of entiation. albumin messenger RNA could be established except that the reduction Albumin and AFP have been localized in livers of carcinogen was most pronounced in nodules which had lost their ability to store treated rats by means of immunohistochemical staining tech glycogen. Since the synthesis and excretion of albumin is a typical niques (5, 21-25). In the present investigation we have studied function of the differentiated hepatocyte in the adult animal, the observed the expression of albumin mRNA by using a powerful in situ decrease in albumin messenger RNA expression in large hepatic nodules hybridization technique in frozen liver sections containing pre is in accordance with the hypothesis of a gradual differentiation or retrodifferentiation of the cell population during carcinogenesis. Hyper- neoplastic and neoplastic lesions induced by treatment of rats plastic nodules produced by continuous treatment of rats with 4-dimeth- with various carcinogens. ylaminoazobenzene showed increased rather than decreased albumin levels. The analysis of albumin messenger RNA expression might there fore be used as a tool to discriminate between nodules of differing MATERIALS AND METHODS biological nature and fate. Treatment of Animals. Female Wistar rats (Zentralinstitut fürVer suchstierkunde, Hannover, Federal Republic of Germany) were used INTRODUCTION throughout the experiments. Treatment with carcinogens was started at an age of 4 weeks. Five rats were given DEN in the drinking water The synthesis and excretion of albumin is one of the major at a dose of 100 mg/liter for 10 days. Following a recovery period of functions of the differentiated hepatocytes in mammalian liver. 10 days they were put on a diet containing 0.05% phénobarbital.The In the adult animal it is produced at high and constant levels time periods of treatment are given in Table 1. One rat was given 4- (1,2). The gene copy number has been shown to be in the range DAB (0.06%) in the diet for 85 days and was killed 2 days later. of one to three/haploid rat genome (1, 3). Variation in the The effects of partial hepatectomy and phénobarbitaltreatment on expression of the gene is not due to permanent changes in gene albumin mRNA expression in liver were investigated in 12-week-old number or structure but modulated by the steady state concen rats. Partial hepatectomy was performed according to the method of Higgins and Anderson (26). Phénobarbital-sodium (80 mg/kg body trations of the specific mRNA molecules (1,2, 4). Albumin is under developmental control. It is not or only minutely ex weight in 0.9% NaCl solution) was administered by i.p. injection. The pressed in the fetus where large quantities of «-fetoprotein are treatment schedules are given in the legend to Fig. 9. Preparation of Tissue Sections. Livers of rats were removed under produced instead, whereas during normal adult life only trace ether anesthesia and immediately frozen at —40"C.Microscope slides amounts of AFP3 are found (5, 6). The levels of albumin were rinsed in acetone prior to use, air dried, and briefly immersed in a 1% solution of serum albumin. Slides were then dried at 70'C and Received 1/28/86; revised 7/17/86; accepted 7/24/86. stored until use. For in situ hybridization, 10-/im frozen sections were The costs of publication of this article were defrayed in part by the payment prepared, mounted on the albumin-coated slides, and air dried (10-20 of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. min). For enzyme and immunohistochemistry freshly prepared cryostat 1This work was partly supported by the Deutsche Forschungsgemeinschaft, sections were used. ATPase activity was stained by the method of Sonderforschungsbereich 302 and by grants from the National Cancer Institute (CA-07575 and CA-22484) (H. C. P.) and is part of the doctoral thesis of G. P. Wachstein and Meisel (27) and GGT activity was stained according to The paper is dedicated to Professor Dr. E. Hecker on the occasion of his 60th the method of Rutenberg et al. (28). Glycogen content was demon birthday. strated by periodic acid-Schiff reaction (29). Slides were counterstained 2To whom requests for reprints should be addressed. with toluidine blue. Immunohistochemical staining for albumin, cyto 3The abbreviations used are: AFP, a-fetoprotein; EH, epoxide hydrolase; 4- chrome P-450 PB2, and EH and staining of NADPH-(cytochrome P- DAB, 4-dimethylaminoazobenzene; DEN, diethylnitrosamine; GGT, -y-glutamyl transpeptidase; cDNA, complementary DNA; PBS, phosphate buffered saline; 450) reducíasewere performed as previously described (16). Rabbit SSC, standard saline citrate (0.15 M sodium chloride: 0.015 M sodium citrate, anti-albumin antiserum was from Bioscience products, Emmenbrucke. pH 7.4). Switzerland. Antisera against the other enzymes were a kind gift of 5903 Downloaded from cancerres.aacrjournals.org on September 30, 2021. © 1986 American Association for Cancer Research. ALBUMIN mRNA DETECTED BY IN SITU HYBRIDIZATION Table 1 Expression of albumin and AFP mRNA, glycogen, and marker enzymes in neoplaslic nodules of rat lirer after start of NADPH Animal12 treatmentGGT186 (days) ATPase storageEH± reducíase P-450PB2+±± AFP±± (10days)DEN +190 -' +± + (a)" (10days)DEN +333 (b)3 +±' ± - ±± (a) (10days)DEN +'333 ++ ±' (b)4 ±±£-Not ± ±± (a) (10days)DEN ±±285 -' -' ± (b)56Carcinogen"DEN +±+• + ±±' (10days)4-DAB +87 +5+ ±+ (continuously)Phénobarbital*YesYesYesYesYesNoKilling +Glycogen + Not ± determinedCytochromedeterminedAlbumin " Carcinogens were given in the drinking water (DEN) or diet (4-DAB) for the time periods indicated. b Phénobarbitalwas given continuously in the diet and was withdrawn 10-14 days before killing except for rats 1, 2. and 5 which were kept on phénobarbitaluntil death. c Data are expressed in relation to surrounding tissue as decreased (- or ), unchanged (±),or increased (+). " More than one large neoplastic nodule is designated by (a) and (b). ' Nonhomogeneous staining. Drs. R. Wolf and F. Oesch, University of Mainz, Federal Republic of Germany. Hybridization Probes. Cloned rat albumin cDNA pRS-1 (30), rat a- fetoprotein cDNA pRAFP 87 (31), and human actin pseudogene cDNA phi 1 (32) were nick translated according to the method of Rigby et al. (33) using 32P-labeled (for RNA dot blots) or 35S-labeled (for in situ hybridization) deoxyribonucleotides (Amersham Buchler, Braun schweig, Federal Republic of Germany). The reaction was run for 1 (32P) to 3 (35S) h using the Bethesda Research Laboratories nick translation kit, 50 ^Ci [32P]dCTP or [35S]dCTP (Amersham Buchler) and 0.2 MgDNA. The resulting specific activities ranged from 2-5 x lO'cpm/Mgof DNA. In Situ Hybridization.
Load more

Recommended publications
  • Nucleic Acids Research
    Volume 1 1 Number 4 1983 Nucleic Acids Research Isolation and nudeotide sequence of a plant tRNA gene: petunia asparagine tRNA Nurit Bawnik, Jacques S.Beckmann*, Sara Sarid + and Violet Daniel Departments of Biochemistry and + Biophysics, The Weizmann Institute of Science, Rehovot 76100, Israel, and *Institute of Field and Garden Crops, The Volcani Center, Bet Dagan 50200, Israel Received 29 October 1982; Revised and Accepted 18 January 1983 ABSTRACT A 14.3 kb petunia genomic DNA fragment was isolated and found to contain a single tRNA gene coding for asparagine tRNA. The nucleotide sequence of the asparagine tRNA gene and its flanking regions has been determined. This gene does not contain intervening sequences nor the 3'-end CCA sequence of the ma- ture tRNA and presents a similar overall sequence homology (70%) to both E. coli and mammalian asparagine tRNA. As in other eukaryotic tRNA genes the 5'-flanking region does not seem to contain any special sequence that could function as a regulatory element and the 3'-end is followed by a short cluster of T that may function as the transcription termination site. INTRODUCTION In contrast to the large amount of information that has been accumulated on the arrangement and structure of tRNA genes from a variety of eukaryotes such as yeast, Neurospora, Bombyx, Xenopus, Drosophila, rat and man (1-13), nothing is known about plant nuclear tRNA genes. To study genes for tRNA in plant cells, we have constructed a petunia genomic library and screened it with an unfractionated cytoplasmic petunia tRNA probe. Here we describe the detailed analysis of a tRNA gene-containing clone and the nucleotide se- Asn quence of a DNA fragment carrying a tRNAMKC(U) gene.
    [Show full text]
  • Nucleotide Sequence of the Cro-Cii-Oop Region of Bacteriophage
    Volume 6 Number 3 March 1979 Nucleic Acids Research Nucleotide sequence of the cro - cll - oop region of bacteriophage 434 DNA Rudolf Grosschedl and Elisabeth Schwarz* Institut f'ur Biologie III der Universitat Freiburg, Schlanzlestr. 1, D-7800 Freiburg i. Br., GFR Received 2 January 1979 ABSTRACT The nucleotide sequence of a 869 bp segment of phage 434 DNA in- cluding the regulatory genes cro and cII is presented and compared with the corresponding part of the phage A DNA sequence. The 434 cro protein as deduced from the DNA sequence is a highly basic protein of 71 amino acid residues with a calculated molecular weight of 8089. While the cro gene sequences of phage 434 and X DNA are very different, the nuc- leotide sequences to the right of the XAim434 boundary show differences only at 11 out of 512 positions. Nucleotide substitutions in the cII gene occur with one exception in the third positions of the respective codons and only one out of several DNA regulatory signals located in this region of the phage genomes is affected by these nucleotide sub- stitutions. INTRODUCTION Bacteriophage 434 is genetically very similar to phage X and together with several others belongs to the group of lambdoid coliphages. All of them share as common characteristics comparable DNA lengths and a similar structural and functional organization of their genomes. Genes coding for biologically related functions quite often are clustered into contiguous groups such as the imunity and replication regions. Because of their functional equivalence, these groups are exchangeable among the various lambdoid phages, resulting in the formation of hybrid bacteriophages such as X imm434 (1), i21 (2) and others.
    [Show full text]
  • "Unnatural Nucleosides with Unusual Base Pairing Properties"
    Unnatural Nucleosides with Unusual Base UNIT 1.4 Pairing Properties Donald E. Bergstrom1 1Purdue University, West Lafayette, Indiana ABSTRACT Synthetic modified nucleosides designed to pair in unusual ways with natural nucleobases have many potential applications in biology and biotechnology. This overview lays the foundation for future protocol units on synthesis and application of unnatural bases, with particular emphasis on unnatural base analogs that mimic natural bases in size, shape, and biochemical processing. Topics covered include base pairs with alternative H-bonding schemes, dimensionally expanded base pairs, hydrophobic base pairs, metal-ligated bases, degenerate bases, universal nucleosides, and triplex constituents. Curr. Protoc. Nucleic Acid Chem. 37:1.4.1-1.4.32. C 2009 by John Wiley & Sons, Inc. Keywords: nucleoside r mimetic r nonpolar r degenerate r universal INTRODUCTION McCutcheon (2007). Extensive efforts over Synthetic modified nucleosides designed to the past decade on DNA-metal base pairs pair in unusual ways with the natural nucleic has spawned three recent reviews in this area acid bases have many potential applications (Shionoya and Tanaka, 2004; Clever et al., in biology and biotechnology. These range 2007; Muller, 2008). from biochemical tools for probing nucleic To design bases that mimic natural bases acid structure or protein-nucleic acid inter- in function, it is useful to consider the fac- actions to tools for re-engineering DNA and tors that are essential for effective base pair- ultimately proteins. Applications as compo- ing and stable duplex formation. In addition nents of nucleic acid—based diagnostic tools to structures that are configured to hydrogen for clinical analysis have been envisioned.
    [Show full text]
  • Download File
    Interaction of DNA Bases with Gold Substrates Marta Rosa, Wenming Sun and Rosa Di Felice∗ Center S3, CNR Institute of Nanoscience, Via Campi 213/A, 41125 Modena, Italy ∗Corresponding author: [email protected] Received 6 December 2012; Accepted 17 December 2012 Abstract The interaction of molecules with inorganic substrates is a crucial issue for applications in molecular electronics. It influences important factors such as the immobilization efficiency and the charge injection through the inter- face. Moreover, mechanical aspects connected to the unfolding of biological molecules are important. We hereby present recent efforts in our group to tackle these problems, based on density functional theory calculations. In particular, we discuss our results on the adsorption of cytosine on Au(111) and on the interaction of guanine, in its natural and size-expanded forms, with small Au clusters. We find that cytosine binds to the Au(111) surface with a mechanism that involves charge sharing, intermediate between chemisorption and physisorp- tion. The investigation of small complexes between guanine and gold clusters reveals the formation of hydrogen bonds: these configurations with unusual bonds are relevant at the corners of nanoparticles, while they can probably be neglected when DNA binds on flat extended metal surfaces. Keywords: DNA/Au interfaces, Density Functional Theory, Van der Waals, DNA modifications, electronic hybridization. Journal of Self-Assembly and Molecular Electronics, Vol. 1, 41–68. doi 10.13052/same2245-4551.112 c 2013 River Publishers. All rights reserved. 42 M. Rosa et al. 1 Introduction The electronic structure of DNA molecules started to attract the attention of scientific research groups because of its implications in DNA damage and repair.
    [Show full text]
  • Modified Nucleic Acids: Replication, Evolution, and Next-Generation Therapeutics Karen Duffy†, Sebastian Arangundy-Franklin† and Philipp Holliger*
    Duffy et al. BMC Biology (2020) 18:112 https://doi.org/10.1186/s12915-020-00803-6 REVIEW Open Access Modified nucleic acids: replication, evolution, and next-generation therapeutics Karen Duffy†, Sebastian Arangundy-Franklin† and Philipp Holliger* Abstract Modified nucleic acids, also called xeno nucleic acids (XNAs), offer a variety of advantages for biotechnological applications and address some of the limitations of first-generation nucleic acid therapeutics. Indeed, several therapeutics based on modified nucleic acids have recently been approved and many more are under clinical evaluation. XNAs can provide increased biostability and furthermore are now increasingly amenable to in vitro evolution, accelerating lead discovery. Here, we review the most recent discoveries in this dynamic field with a focus on progress in the enzymatic replication and functional exploration of XNAs. Nucleic acids: natural and expanded functions afford completely new modalities for therapeutic inter- A heritable genetic system is a defining requirement for vention (e.g., direct protein expression or specific and life. The natural nucleic acids, DNA and RNA, are ex- programmable modulation of gene expression) that can- quisitely suited to store and propagate genetic informa- not be easily accessed by other biologics or small mol- tion with sufficient stability and fidelity to support large ecule drugs. However, several challenges have thus far genomes but also the flexibility to enable evolution. Nu- prevented nucleic acids from reaching their full potential cleic acids are unique among biopolymers in that both as medicines including poor chemical and biological accessible information and functional capacity coexist in stability and narrow chemical diversity. a single molecule.
    [Show full text]
  • Synthetic Biology: Cellular Xdna Readers
    research highlights DRUG DISCOVERY this state, Ooi et al. treated cells with dimethyl Science, published online 17 November 2011, fumarate and found increased expression Malaria under arrest doi:10.1126/science.1211936 of NRF2 and its target genes, suggesting that KEAP1, which is known to control the The malaria parasites from Plasmodium species have both a liver stage (EEF or sporozoite stability of NRF2, is sensitive to fumarate. MS stage) and a blood stage in human hosts. Because EEFs of certain Plasmodium strains can in both studies confirmed the modification of remain dormant for long periods and disease symptoms originate during the blood stage, several residues in KEAP1 to S-(2-succinyl)- therapeutics that can eliminate parasites of both stages are needed, yet most drugs target cysteine (2SC) in fumarate-accumulating only the blood-stage parasites. To find drugs that might inhibit multiple parasite stages, conditions. Ooi et al. further demonstrated Meister et al. screened a library of 5,697 compounds known to be active against P. falciparum that ubiquitinated KEAP1 was elevated in blood stages. In a high-content imaging assay of sporozoite-infected cultured liver cells, the FH-deficient cells compared to controls; authors found 275 compounds that are structurally unrelated to known antimalarial scaffolds additional experiments using proteasomal and decrease parasite size within cells after infection. They then used a hypergeometric inhibitors led to a model where 2SC-modified mean function to determine which of the 2,715 independent chemical-scaffold clusters KEAP1 is ubiquitinated and degraded by a in the original library were fully represented among the hits, identifying quinazoline, the proteasome.
    [Show full text]
  • Template-Independent Enzymatic Oligonucleotide Synthesis (Tieos): Its History, Prospects, and Challenges Michael A
    Perspective Cite This: Biochemistry XXXX, XXX, XXX−XXX pubs.acs.org/biochemistry Template-Independent Enzymatic Oligonucleotide Synthesis (TiEOS): Its History, Prospects, and Challenges Michael A. Jensen*,† and Ronald W. Davis†,‡ † Stanford Genome Technology Center, Department of Biochemistry, Stanford University, Palo Alto, California 94304, United States ‡ Department of Genetics, Stanford University, Palo Alto, California 94304, United States ABSTRACT: There is a growing demand for sustainable methods in research and development, where instead of hazardous chemicals, an aqueous medium is chosen to perform biological reactions. In this Perspective, we examine the history and current methodology of using enzymes to generate artificial single- stranded DNA. By using traditional solid-phase phosphoramidite chemistry as a metric, we also explore criteria for the method of template-independent enzymatic oligonucleotide synthesis (TiEOS). As its key component, we delve into the biology of one of the most enigmatic enzymes, terminal deoxynucleotidyl transferase (TdT). As TdT is found to exponentially increase antigen receptor diversity in the vertebrate immune system by adding nucleotides in a template- free manner, researchers have exploited this function as an alternative to the phosphoramidite synthesis method. Though TdT is currently the preferred enzyme for TiEOS, its random nucleotide incorporation presents a barrier in synthesis automation. Taking a closer look at the TiEOS cycle, particularly the coupling step, we find it is comprised of additions > n+1 and deletions. By tapping into the physical and biochemical properties of TdT, we strive to further elucidate its mercurial behavior and offer ways to better optimize TiEOS for production-grade oligonucleotide synthesis. n the mid-20th century, there were several key break- toward “green” technology development in terms of sustainable I throughs in the fields of genetics and biochemistry that have chemistry.14 Possible advantages of using an enzyme for ssDNA led us to current day medicine.
    [Show full text]
  • Efficient Replication Bypass of Size-Expanded DNA Base Pairs in Bacterial Cells** James C
    Zuschriften DOI: 10.1002/ange.200805683 Unnatural DNA Bases Efficient Replication Bypass of Size-Expanded DNA Base Pairs in Bacterial Cells** James C. Delaney, Jianmin Gao, Haibo Liu, Nidhi Shrivastav, John M. Essigmann,* and Eric T. Kool* One of the long-standing aims of biomimetic chemistry has The four size-expanded DNA base pairs are shown in been to develop molecules that function as much as possible Figure 1. The hydrogen-bonded pairs involve benzo homo- as the natural ones do.[1] Among biopolymers, nucleic acids logues opposite complementary natural partners, yielding have long served as a test bed for bioinspired design. The base pairs 2.4 larger than Watson–Crick pairs.[6] The earliest focus of altered structures for DNA was the redesign of the phosphodiester backbone,[2] and numerous studies found backbone variants that assembled well into helices. Moreover, in recent years, some altered sugars have also been shown to be substrates for certain polymerase enzymes.[3] Such work suggests the possibility of future biological activities associated with altered DNA backbones. More recently, a number of laboratories have focused on design of replacements for the DNA bases themselves, which encode the chemical information of the cell.[4] This is a challenging goal, because biological enzymes have evolved the ability to manipulate these bases and base pairs with extraordinarily high selectivity. The polymerase replication of designed DNA pairs has been studied in a number of laboratories. Significant successes have been reported using varied strategies, including base pairs with altered hydrogen- bonding patterns,[4a] pairs that lack hydrogen bonds altoge- ther,[4b–e,i,j,m–p] and pairs that have larger-than-natural dimen- Figure 1.
    [Show full text]
  • Expanded DNA Base Pairs in Bacterial Cells
    Efficient replication bypass of size- expanded DNA base pairs in bacterial cells The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. Citation Delaney, JamesC. et al. “Efficient Replication Bypass of Size- Expanded DNA Base Pairs in Bacterial Cells.” Angewandte Chemie International Edition 48.25 (2009): 4524–4527. As Published http://dx.doi.org/10.1002/anie.200805683 Publisher Wiley Blackwell Version Author's final manuscript Citable link http://hdl.handle.net/1721.1/75427 Terms of Use Creative Commons Attribution-Noncommercial-Share Alike 3.0 Detailed Terms http://creativecommons.org/licenses/by-nc-sa/3.0/ NIH Public Access Author Manuscript Angew Chem Int Ed Engl. Author manuscript; available in PMC 2012 September 06. NIH-PA Author ManuscriptPublished NIH-PA Author Manuscript in final edited NIH-PA Author Manuscript form as: Angew Chem Int Ed Engl. 2009 ; 48(25): 4524–4527. doi:10.1002/anie.200805683. Efficient Replication Bypass of Size-expanded DNA Base Pairs in Bacterial Cells** Dr. James C. Delaney, Departments of Chemistry and Biological Engineering, Massachusetts Institute of Technology Cambridge, MA 02139 USA Dr. Jianmin Gao, Department of Chemistry, Stanford University, Stanford, CA 94305 USA Dr. Haibo Liu, Department of Chemistry, Stanford University, Stanford, CA 94305 USA Nidhi Shrivastav, Departments of Chemistry and Biological Engineering, Massachusetts Institute of Technology Cambridge, MA 02139 USA Prof. Dr. John M. Essigmann, and Departments of Chemistry and Biological Engineering, Massachusetts Institute of Technology Cambridge, MA 02139 USA Prof. Dr. Eric T. Kool Department of Chemistry, Stanford University, Stanford, CA 94305 USA John M.
    [Show full text]
  • The Enzymatic Repair of Dna, I. Formation of Circular Xdna* by Malcolm L
    THE ENZYMATIC REPAIR OF DNA, I. FORMATION OF CIRCULAR XDNA* BY MALCOLM L. GEFTER, ANDREW BECKER, AND JERARD HURWITZ DEPARTMENT OF DEVELOPMENTAL BIOLOGY AND CANCER, ALBERT EINSTEIN COLLEGE OF MEDICINE, BRONX, NEW YORK Communicated by Alfred Gilman, April 19, 1967 Physical and genetic studies on bacteriophages indicate that genetic recombina- tion involves the breakage and reunion of DNA molecules." 2 It has been postu- lated that the process of reunion occurs first by the formation of a joint structure containing portions of the parental DNA's linked together by noncovalent bonds, and second by the formation of a phosphodiester "seal" between the internal ter- mini of DNA chains held together in this manner.2 In our search for the enzyme(s) responsible for the covalent joining of polynucleotides, we anticipated that phage XDNA, in the hydrogen-bonded circular form ("Hershey circle"),3 might provide a model system for studying the enzymatic fate of DNA termini held in apposition by hydrogen bonds. In agreement with the results reported by Gellert,4 it was found that extracts of Escherichia coli catalyzed the formation of covalently closed circles from hydrogen-bonded circles of XDNA. We have purified this enzyme activity from E. coli K12 approximately 2000-fold. The enzyme also catalyzes the conversion of XDNA containing single-strand breaks to a form which, in al- kaline sucrose gradients, sediments in a manner indistinguishable from intact XDNA. The enzyme requires double-stranded DNA as substrate and is dependent on a heat-stable factor. Similar results have been observed by Olivera and Leh- man.5 Materials and Methods.-Bacterial strains and bacteriophages: E.
    [Show full text]
  • Complete Nucleotide Sequence of Torpedo Marmorata Mrna
    Proc. Natl. Acad. Sci. USA Vol. 81, pp. 7313-7317, December 1984 Biochemistry Complete nucleotide sequence of Torpedo marmorata mRNA coding for the 43,000-dalton v2 protein: Muscle-specific creatine kinase (cDNA cloning/hybrid-selected translation/oligonucleotide-specific primer extension) JRR6ME GIRAUDAT*, ANNE DEVILLERS-THIERY*, JEAN-CLAUDE PERRIARDt, AND JEAN-PIERRE CHANGEUX* *Unitd de Neurobiologie Moldculaire et Laboratoire Associd 270, Centre National de la Recherche Scientifique Interactions Moldculaires et Cellulaires, Institut Pasteur, 25 rue du Docteur Roux, 75 015 Paris, France; and lInstitute for Cell Biology, Swiss Federal Institute for Technology, Honggerberg, CH 8093 Zurich, Switzerland Contributed by Jean-Pierre Changeux, August 1, 1984 ABSTRACT DNA sequences coding for the muscle-specif- charge. We previously reported the construction of cDNA ic subunit of creatine kinase have been isolated from cDNA libraries from Torpedo marmorata electric organ, which en- libraries constructed from Torpedo marmorata electric organ. abled us to isolate cDNA clones coding for the a-subunit of Clones were screened by differential in situ hybridization and the acetylcholine receptor (12, 13). We describe here the iso- hybrid-selected translation. The in vitro translation product of lation of cDNA clones coding for the T. marmorata muscle- the selected mRNA was immunoprecipitated by anti-chicken specific CK and report the complete nucleotide sequence of creatine kinase antibodies and comigrated with Torpedo mus- the corresponding mRNA. cle creatine kinase on two-dimensional gels at the same posi- tion as the cytosolic 43,000-dalton protein referred to as v2. MATERIALS AND METHODS The cDNA inserts hybridized to a mRNA species present in Materials.
    [Show full text]
  • Local Aromaticity in Natural Nucleobases and Their Size-Expanded Benzo-Fused Derivatives
    J. Phys. Chem. A 2006, 110, 12249-12258 12249 Local Aromaticity in Natural Nucleobases and Their Size-Expanded Benzo-Fused Derivatives Oscar Huertas,† Jordi Poater,‡ Miguel Fuentes-Cabrera,§ Modesto Orozco,| Miquel Sola`,*,⊥ and F. Javier Luque*,† Departament de Fisicoquı´mica, Facultat de Farma`cia, UniVersitat de Barcelona, AVenida Diagonal 643, 08028, Barcelona, Spain, Afdeling Theoretische Chemie, Scheikundig Laboratorium der Vrije UniVersiteit, De Boelelaan 1083, NL-1081 HV Amsterdam, The Netherlands, Center for Nanophase Material Sciences and Computer Science and Mathematics DiVision, Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN, 37831-6494, Departament de Bioquı´mica i Biologia Molecular, Facultat de Quı´mica, UniVersitat de Barcelona, Martı´ i Franque´s 1, 08028 Barcelona, Spain, Unidad de Modelizacio´n Molecular y Bioinforma´tica, Parc Cientı´fic de Barcelona, Josep Samitier 1-6, 08028 Barcelona, Spain, Computacional Biology Program, Barcelona Supercomputing Center. Edificio Nexus II. Barcelona 08028, Spain, and Institut de Quı´mica Computacional and Departament de Quı´mica, UniVersitat de Girona, 17071 Girona, Spain ReceiVed: June 18, 2006; In Final Form: August 29, 2006 The influence of the insertion/addition of a benzene ring to the natural nucleic acid bases on the local aromaticity of the so-called size-expanded (xN, with N being adenine, guanine, cytosine, and thymine) bases is examined. To this end, the local aromaticity of the six- and five-membered rings in both the natural bases and their benzoderivatives is determined using HOMA, NICS, aromatic fluctuation index (FLU), and para-delocalization index (PDI) descriptors. In general, there is a good correspondence between the different indices, so that ring moieties with more negative NICS values also have larger HOMA and PDI measures and lower FLU indices.
    [Show full text]