Data in Brief 7 (2016) 1038–1044
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Data in Brief
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Data Article Data supporting characterization of CLIC1, CLIC4, CLIC5 and DmCLIC antibodies and localization of CLICs in endoplasmic reticulum of cardiomyocytes
Devasena Ponnalagu a, Shubha Gururaja Rao a, Jason Farber a, Wenyu Xin a, Ahmed Tafsirul Hussain a, Kajol Shah a, Soichi Tanda b, Mark A. Berryman c, John C. Edwards d, Harpreet Singh a,n a Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA 19102, United States b Department of Biological Sciences, Ohio University, Athens, OH 45701, United States c Department of Biomedical Sciences, Ohio University, Athens, OH 45701, United States d Division of Nephrology, St. Louis University, St. Louis, MO 63110, United States article info abstract
Article history: Chloride intracellular channel (CLICs) proteins show 60–70% Received 22 January 2016 sequence identity to each other, and exclusively localize to the Received in revised form intracellular organelle membranes and cytosol. In support of 22 February 2016 our recent publication, “Molecular identity of cardiac mito- Accepted 16 March 2016 chondrial chloride intracellular channel proteins” (Ponnalagu Available online 26 March 2016 et al., 2016) [1], it was important to characterize the specificity Keywords: of different CLIC paralogs/ortholog (CLIC1, CLIC4, CLIC5 and Chloride intracellular channels DmCLIC) antibodies used to decipher their localization in car- Endoplasmic reticulum diac cells. In addition, localization of CLICs in the other orga- Mitochondria nelles such as endoplasmic reticulum (ER) of cardiomyocytes Cardiomyocytes was established. This article also provides data on the different primers used to show the relative abundance of CLIC paralogs in cardiac tissue and the specificity of the various CLIC antibodies used. We demonstrate that the predominant CLICs in the heart, namely CLIC1, CLIC4 and CLIC5, show differential
DOI of original article: http://dx.doi.org/10.1016/j.mito.2016.01.001 n Corresponding author. E-mail address: [email protected] (H. Singh). http://dx.doi.org/10.1016/j.dib.2016.03.061 2352-3409/& 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). D. Ponnalagu et al. / Data in Brief 7 (2016) 1038–1044 1039
distribution in endoplasmic reticulum. CLIC1 and CLIC4 both show co-localization to the endoplasmic reticulum whereas CLIC5 does not. & 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Specifications Table
Subject area Biology More specific Cardiac intracellular ion channels subject area Type of data Table, microscopy, Western blots and text files How data was Microscope (Olympus IX81), Western blotting acquired Data format Filtered, analyzed Experimental Specificity of CLIC antibodies using specific knock out mice and clic109 mutant flies. factors Experimental Confocal microscopy to determine the localization of CLICs in cardiac endoplasmic features reticulum, specificity of the antibody used is checked by Western blotting using specific CLIC KO mouse cardiac tissues and Drosophila mutant cardiac tubes. Data source Laboratory animals from Charles Rivers, PA location Data accessibility Data is with this article
Value of the data