bioRxiv preprint doi: https://doi.org/10.1101/2020.02.15.950782; this version posted February 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Oecophyllibacter saccharovorans gen. nov. sp. nov., a 2 bacterial symbiont of the weaver ant Oecophylla smaragdina 3 with a plasmid-borne sole rrn operon 4 Kah-Ooi Chuaa, Wah-Seng See-Tooa, Jia-Yi Tana, Sze-Looi Songb, c, Hoi-Sen Yonga, Wai-Fong 5 Yina, Kok-Gan Chana,d* a Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia b Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia c China-ASEAN College of Marine Sciences, Xiamen University Malaysia, 43900 Sepang, Selangor, Malaysia d International Genome Centre, Jiangsu University, Zhenjiang, China * Corresponding author Email address:
[email protected] 6 Keywords: 7 Acetobacteraceae, Insect, Whole genome sequencing, Phylogenomics, Plasmid-borne rrn operon 8 9 Abstract 10 In this study, acetic acid bacteria (AAB) strains Ha5T, Ta1 and Jb2 that constitute the core microbiota 11 of weaver ant Oecophylla smaragdina were isolated from multiple ant colonies and were distinguished 12 as different strains by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass 13 spectrometry and distinctive random-amplified polymorphic DNA (RAPD) fingerprints. These strains 14 showed similar phenotypic characteristics and were considered a single species by multiple delineation 15 indexes. 16S rRNA gene sequence-based phylogenetic analysis and phylogenomic analysis based on 16 96 core genes placed the strains in a distinct lineage in family Acetobacteraceae.