Polyclonal Antibody to EEF1E1 (N-Term) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP32965PU-N Polyclonal Antibody to EEF1E1 (N-term) - Aff - Purified

Alternate names: AIMP3, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Elongation factor p18, Eukaryotic translation elongation factor 1 epsilon-1, Multisynthetase complex auxiliary component p18, P18 Quantity: 0.1 mg Concentration: 0.5 mg/ml Uniprot ID: O43324 NCBI: NP_004271.1 GeneID: 9521 Host: Goat Immunogen: Peptide with sequence from the internal region (near N-Terminus) of the protein sequence according to NP_004271.1; NP_001129122.1. AA Sequence: C-SKGNKYSAQGERQ

Format: State: Liquid purified Ig fraction Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide Buffer System: Tris saline, pH~7.3 Preservatives: 0.02% Sodium Azide Stabilizers: 0.5% BSA Applications: Peptide ELISA: 1/16000 (Detection Limit). Western blot: 0.1-0.3 µg/ml. Approx 19kDa band observed in lysates of cell line HepG2 and K562 (calculated MW of 19.8kDa according to NP_004271.1). Immunohistochemistry on Paraffin Sections: 5 µg/ml. Paraffin embedded Human Pancreas. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody is expected to recognize both reported isoforms (NP_004271.1; NP_001129122.1). Species Reactivity: Tested: Human, Pig. Expected from sequence similarity: Canine, Bovine. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20180604 1 / 3 AP32965PU-N: Polyclonal Antibody to EEF1E1 (N-term) - Aff - Purified

Pictures: AP32965PU-N (0.1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP32965PU-N (0.3µg/ml) staining of Pig Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20180604 2 / 3 AP32965PU-N: Polyclonal Antibody to EEF1E1 (N-term) - Aff - Purified

AP32965PU-N(5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20180604 3 / 3