MCM3 Antibody Cat. No.: 25-374

MCM3 Antibody

Antibody used in IHC on Human brain at 5 ug/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human MCM3.

TESTED APPLICATIONS: ELISA, WB

MCM3 antibody can be used for detection of MCM3 by ELISA at 1:312500. MCM3 antibody APPLICATIONS: can be used for detection of MCM3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) 721_B Cell Lysate

PREDICTED MOLECULAR 91 kDa WEIGHT:

October 2, 2021 1 https://www.prosci-inc.com/mcm3-antibody-25-374.html Properties

PURIFICATION: Antibody is purified by peptide affinity chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: MCM3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: MCM3

ALTERNATE NAMES: MCM3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB

ACCESSION NO.: NP_002379

PROTEIN GI NO.: 394582093

GENE ID: 4172

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

October 2, 2021 2 https://www.prosci-inc.com/mcm3-antibody-25-374.html MCM3 is one of the highly conserved mini- maintenance (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has BACKGROUND: been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

REFERENCES: 1) Lin, D.I., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (23), 8079-8084.

ANTIBODIES FOR RESEARCH USE ONLY.

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