National Mission on Himalayan Studies (NMHS) NMHS Annual Progress Report – Pro forma

Kindly fill the NMHS Annual Progress Report segregated into following 11 segments, as applicable to the objectives & quantifiable outcomes of your NMHS Project.

1. Project Information 2. Project Site Details 3. Project Activities Chart w.r.t. Timeframe [Gantt or PERT] 4. Financial and Resource Information 5. Equipment and Asset Information 6. Expenditure Statement and Utilization Certificate (UC) 7. Project Beneficiary Groups 8. Project Progress Summary (as applicable to the project) 9. Project Linkages (with concerned Institutions/ State Agencies) 10. Knowledge Products – Publication, recommendations, etc. 11. Project Concluding Remarks

Kindly attach a descriptive Annexure/ Files separately for the segments marked for the detailed description required. Please let us know in case of any query at: [email protected]

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NMHS Progress Report (Period from 01st April-2020 to 31st March-2021) 1. Project Information Project ID: GBPNI/NMHS-2018-19/MG 06 Sanction Date: 12-12-2018 Project Title: “Ex-situ conservation and development of gene bank of commercially important threatened medicinal plants in the high altitude areas, " BTG: Conservation of Biodiversity

PI and Affiliation Dr. Ashok Singh, Senior Scientist, Environmental Technology Division, CSIR- (Institution): Institute of Himalayan Bioresource Technology Palampur-176 061, HP, Name & Address Co-PI: Partner Institute: of the Co-PI, if 1. Dr. Sarla Shashni, Scientist D, G.B. Pant National Institute of Himalayan any: Environment, Himachal Region Centre, Mohal-Kullu, HP-175126 Other Co-PIs: 1. Dr. Dinesh Kumar, Senior Scientist, Chemical Technology Division, CSIR-IHBT Palampur (H.P.): Ph. 9459344184; E-mail: [email protected] 2. Dr. Ashish R. Warghat, Senior Scientist, Biotechnology Division, CSIR-IHBT Palampur (H.P.): E-mail: [email protected]; Ph. 9463378921 3. Dr. Amit Kumar, Senior Principal Scientist, Environmental Technology Division, CSIR-IHBT Palampur (H.P.): E-mail: [email protected]; Ph.: 9418039091 4. Dr. Amit Chawla, Principal Scientist, Environmental Technology Division, CSIR- Institute of Himalayan Bioresource Technology Palampur-176 061, HP, India; E- mail: [email protected]: Ph. 9418076217; 01894233339 (437) 5. Dr. Ram Kumar Sharma, Senior Principal Scientist, Biotechnology Division, CSIR- Institute of Himalayan Bioresource Technology Palampur-176 061, HP, India (H.P.): E-mail: [email protected]; Ph.: 9418031964 6. Dr. Sanatsujat Singh, Senior Principal Scientist, Agrotechnology Division,: CSIR- Institute of Himalayan Bioresource Technology Palampur-176 061, HP, India (H.P.): E-mail: [email protected]; Ph.: 9418709582

Structured Background Abstract - The rapid increase in the demand of herbal species for preparation of different detailing the formulation against of various disease were observed at the global level. Hence current year over-overexploitation of the commercially important plants has been experienced. progress Which resulted in to the increase of RET plants. [Word Limit 250 words]: Ecological as well as field gene bank studies were carried out as per the project objectives and target assigned. The ex-situ conservation of selected medicinal plants with the aim to convert RET plants in to non RETs were focused in the present study. Seeds/seedlings/rhizomes of different accession of the Angelica glauca, Carum carvi and Arnebia euchroma were collected from the different locations of the Western Himalaya (Lahaul-Spiti, Kullu, Shimla, Chamba, and Chamoli regions). Collected accession has been planted in the nursery of the Ribling (Lahual), and G B Pant Institute (Kullu) and well established. It also being propagated through conventional (seeds/rhizomes) and tissue culture methods, and efforts are in the way of mass multiplication. After achieving of the successful methodology for propagation by conventional and tissue culture practices for selected species, we have been able to transfer the technology for cultivation by seeds of Carum carvi with testing of different accession, and produces large seedlings. With our efforts, today we are able to conserve the nurseries of Carum carvi in the farmer’s field (about 40 farmers) at high altitude of Manali, Lahual and Bathad panchayat of Tirthan valley selected for generating nursery of the plants. Interacted with more than 03 Vaidyas/ herbal NMHS 2020 NMHS-Annual Progress Report (APR) Pro Forma Page 2 of 27

practitioners and recorded the use of targeted medicinal plants (used by these herbal practitioners). Objectives/ Aim: studies of selected wild populations and collection of different accessions of selected commercially important threatened medicinal plants Establishment of a gene bank, field evaluation, chemical and molecular characterization of target species Selection of elite accessions from the cultivated species, their multiplication (by conventional and tissue culture techniques) Approach: Biotechnological techniques: Invitro propagation, Cryopreservation Medicinal Plants (MPs) multiplied through Conventional methods Characterization for selection: Chemical methods/ molecular and physical basis Results: Ecological and morphological study of targeted medicinal plants under project has been done from different locations of Western Himalaya. Targeted medicinal plants multiplied through Micro and Macro propagation. Successfully organized 02 awareness workshop/seed-plants distribution program Observed ecological parameters and marked the surveyed site with GPS system.

Samples of plants collected from different sites of HP & their plantation records at CeHAB genebank has been done: Conserved a total of 10 accessions of Angelica glauca plants in CeHAB field Conserved a total of 13 accessions of Arnebia euchroma in CeHAB field Conserved a total of 09 accessions of Carum carvi in the CeHAB field Conserved a total 1600 nos. of plants and seeds sown in 6000 polybags of Angelica glauca plants in CeHAB field by conventional method Conserved a total 1050 nos. of plants and seeds sown in 2000 polybags of Arnebia euchroma in the CeHAB field by conventional method. Conserved a total 20000 nos. of Carum carvi plants in CeHAB field by conventional method Also, with availability of planting materials (raised through conventional/tissue culture method) of different accessions of Angelica glauca and Carum carvi were sown in the four different location based nurseries at CeHAB farm, GBPNIHE Mohal, Kullu and CSIR-IHBT Palampur, Bathad Panchayat Kullu. Conclusion: Sparsely distributed populations and less availability of selected commercially threatened species like Angelica glauca, Arnebia euchroma and Carum carvi in the Western Himalaya has showed a scope for their mass conservation and characterization for the elite germplasm selection. Less density of Angelica glauca and Arnebia euchroma has been found. However, Carum carvi species showed sufficient density and restricted in the few habitats. The information on habitat types will be helpful for the prioritisation of species their conservation management and scope for application in the field on Biotechnology and Agro-technology. Majority of the selected species were successfully conserved in the field genebanks/farmers field and likely to be transplanted in their natural habitats. Also selected elite accessions of conserved targeted species in the field genebank were done. These results could be used as baseline for selection of suitable cultivars and a conversion step towards RET species into non-RETs status, development of agro techniques and a step for the future conservation strategy. Better establishment of plants by seeds of Carum carvi indicates well adaptation of this species under ex-situ conservation and has a commercial potential for the cultivation in future.

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Recommendations: The preliminary studies indicates that Carum carvi can grow well by conventional and tissue culture technique in the lowest altitude upto 1300 m asl at IHBT Palampur, GBPNIHE nursery at Dohranalla and highest altitude up to 3500 m asl at CeHAB Ribling conditions. Angelica glauca (a threatened species) can grow easily by the rhizome cuttings and showed a very less germination establishment by seeds in the CeHAB Ribling (3450 m asl) and CSIR-IHBT Palampur by conventional method. Standardized tissue culture protocol for the Angelica galuca and Arnebia euchroma showed promising initial results for conservation.

Target Role Role & Responsibilities Partner-I (CSIR-IHBT Partner-II (GBPNIHE, Mohal Partner- Palampur) Kullu) III (HP State Forest Departme nt) Identification of Population assessment, Population assessment, identification Endorsed us potential natural identification and collection of and collection of planting materials of to give help habitat of RET planting materials of Arnebia Arnebia euchroma, Angelica glauca, during the populations and euchroma, Angelica glauca, Carum carvi from minimum 6 habitat project their Carum carvi from minimum 08 populations of each species from the period morphological habitats each species from the sub-temperate and temperate zone studies high altitude (dry temperate, altitude zone of the western Himalaya alpine) zone of the western Himalaya Database on Dataset on morphological Dataset on field data for morphological genetically variations, GPS coordinates, variations, GPS, Habitats, Altitude in diverse accessions Habitats, Altitude range of the wild habitats. Quality planting of selected species species in their wild and to study material supply to the field genebank for conservation growth pattern in the field sites at CSIR-IHBT CeHAB and genebank at CeHAB IHBT for chemical/ molecular studies Establishment of Field preparation, layout of the Help to strengthen and establishment field Genebank at genebank, agro-technological of field Genebank at CSIR-IHBT CSIR-IHBT intervention and establishment of Centre for High Altitude Biology at Centre for High genebank Ribling, (L&S) Altitude Biology at Ribling (L&S) Selection of elite Responsible Responsible accessions from the ex-situ genebank Large scale Target to cultivate 70000 plants at Mass multiplication with a target to multiplication of CSIR-IHBT CeHAB farm and cultivate 30000 plants by conventional targeted demonstration sites propagation protocols medicinal plants through conventional and tissue culture technique Database on Responsible - molecular level status of selected accessions

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Characterization Performance in the field genebank Morphological studies of the wild (physical and and wild populations populations chemical) to select elite accessions Organizing Venue deciding and with expert Organize or help organizing Capacity Seminar, resource persons Building programs conference, workshop

2. Project Site Details Project Site: Western Himalaya: High Altitude area in Himachal Pradesh; Cold desert area in H.P. IHR States Covered: Himachal Pradesh, Uttarakhand Long. & Lat.: Annexure I, 31.1048° N, 77.1734° E Site Maps* (3 No.) Annexure II Site Photographs* (No.): Annexure III *Attach a separate Descriptive Annexure/ Files (.JPG, .TIFF, etc.). 3. Project Activities Chart w.r.t. Timeframe [Gantt or PERT]

Project activities Description of work undertaken (year 2020-2021) Quantifiable outputs Ecological and Target species: Angelica glauca, Arnebia euchroma, Carum carvi Morphological morphological Ecological and morphological studies: Surveyed sites like Tindi, parameter studies and studies in the Salgran, Kukumseri, Naingar, Rohru, Jobrang, waterfall Physical parameters Wild Habitats of side, Rarik, Salpat, Khanjhar, Changut, Karpat, Lobber, studies in the wild Himachal Pradesh Khanghsar, Dorni, Yangla, Sissu, Kibber, Chicham, Hikkim, populations Lossar, Sirla Gompa, near Rohtang tunnel, Beeling ghar, Dalag respectively maidan, Gwadhi Nallah, Thorang nallah, Gondla, Purat, Khorpani (Annexure I & IV) & Ropsang, Jagatsukh, Yurnath, Rahlafall, Khas village Chamoli etc. and collected sample accessions for the field Genebank establishment Mapping of Total 30 location has been selected for population assessment of the GIS mapping of the collection sites species (13 for Arnebia euchroma, 09 for Carum carvi and 10 for wild population Angelica glauca) (Annexure II) Establishment of Field Genebank establishment at CeHAB Ribling, Lahaul-Spiti: Ex-situ conservation / a Gene Bank This year we sown a total area of 660 sqm under conservation. Till Mass propagation by now we have conserved Angelica glauca (10 accessions, 1600 plants the conventional and seeds sown in 6000 polybags); Arnebia euchroma (13 method in the CeHAB accessions, 1050 plants and seeds sown in 2000 polybags); Carum Ribling (Annexure V carvi (09 accessions, seeds 1.5 kg). VI &VII) Molecular Molecular studies of Angelica glauca (07 population accessions) & Final compilation marker studies for Carum carvi (100 gm seeds) is done work is going on identification of elite accessions The sample for Arnebia euchroma: Root and root scalp of Rarik, Kibber (Annexure VIII) analysis of accessions shows the presence of Shikonin and their derivatives chemical active while there is no presence of Shikonin and derivatives in upper parts ingredient (i.e. leaf) samples analysis of Carum carvi: Samples of 6 accessions showed that the presence of cultivated and Di –hydrocarvone and carvone ranging from (58-62%) & (30-34%) wild accessions respectively populations Angelica glauca: oil extraction from roots of 5 accessions, and simultaneously identified the chemical compounds by UPLC

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4. Financial and Resource Information Note: A separate bank account is expected to be opened for NMHS Project as per the provision of Direct Beneficiary Account (DBA) as laid out by the Govt. of India and also facilitate the audit of accounts. The interest earned out of the NMHS project funds should be reported clearly in the utilization certificate. Total Grant: 69,61,760.00 Lacs Grant Received Rs: 23.63005 Lacs Date: December, 2020

Project Affiliations/ Institution Budget Work Done by each Partner(s) Allocated to Project Partner (Rs. Lacs) Year 2020-21 Partner 1 CSIR-Institute of Himalayan Bioresource Technology, 16.43005 [Name of descriptive PO-6, Palampur, Himachal Pradesh 177 061 Annexure/ File attached separately] Partner 2 G. B. Pant National Institute of Himalayan 7.20 [Name of descriptive Environment, Himachal Regional Centre, Mohal-Kullu, Annexure/ File HP, 175 126) attached separately]

Project Staff Information: S. No. Name Qualification Designation Fellowship/ Wages paid Remarks 1. Mr. Rajat MSc. JPF Rs. 20,000+HRA 8% PM Working at IHBT Bhardwaj Biotechnology 2. Mr. Abhishek Ph.D. SRF Rs. 31,000 +HRA 8% PM Working at Biotechnology GBPNIHE

5. Equipment and Asset Information S. No. Equipment Name Details Cost Date of Photographs Lowest (Qty) (Make/ Installation of Quotation, if not Model) Equipment* purchased 1. Shade-house Iron Angle, 2,00,000 1/02/2021 Attached Work almost polysheet, completed but greenshade / Payment is yet to local made be released Note: Attach a Descriptive Annexure/ File separately. 6. Expenditure Statement and Utilization Certificate Please update the annual Expenditure Statement and Utilization Certificate (UC) periodically. Expenditure Information: CSIR-IHBT: (Implementing agency) Expenditure Statement (Dr. Ashok Singh, Sr. Scientist, PI) S. Financial Position/Budget Opening Funds Expenditure % of Total No Head balance as on Sanctioned cost . 01.04.2020 (2020-21) I Salaries/Manpower cost 1,33,800 6,48,000 6,82,642 87.317

II Travel 2,081 2,25,000 2,27,081 100.00 III Expendables &Consumables 4,033 2,95,000 2,99,033 100.00 IV Contingencies 2,461 1,00,000 69,268 67.604

V Activities & Other Project cost 0 7,45,005 7,48,368 100.451

VI Institutional Charges 0 1,50,000 1,50,000 100.00 VII Equipments 350 2,00,000 Greenhouse Payment work Likely to completed be released NMHS 2020 NMHS-Annual Progress Report (APR) Pro Forma Page 6 of 27

payment left (about Rs. ~200000 99.00% ) Total 1,42,725 23,63,005** 21,76,392 ~94.838% Interest accrued 4,995 9,027 Grand Total 1,42,725* 23,72,032 * excluding interest ** Out of the total fund received Rs. 23,63,005 for the year 2020-21, we have transferred a sum of Rs. 7,20,000 to our project partner GBPNIHE Himachal Regional Centre, Mohal-Kullu (H.P.).

GBPNIHE Himachal Regional Centre, Mohal-Kullu (H.P.): (Partner Organization) Expenditure statement by (Dr. Sarla Shashni, Scientist D, PI) S. Financial Position/Budget Opening Funds Expenditure % of Total No Head balance as on Sanctioned cost . 01.04.2020 (2020-21) I Salaries/Manpower cost 97,161 3,24,000 2,54,880 60.518 II Travel 13,203 1,00,000 32,875 29.041 III Expendables &Consumables 65,193 75,000 1,07,410 76.616 IV Contingencies 17,871 25,000 11,573 26.995 V Activities & Other Project cost 91,222 1,50,000 94,579 39.208 VI Institutional Charges 0 46,000 46,000 100.00 VII Equipments 0 0 0 0.00 Total 2,84,650 7,20,000 5,47,317 54.478% Interest accrued - - Grand Total 2,84,650 7,20,000

Period Expenditure Statement* Utilization Certificate (UC)* Annual Attached the Statement of Expenditure Attached the Utilization Certificate [Attach in the prescribed format] [Attach in the prescribed format]

*Attach the descriptive Annexure/ File in the prescribed NMHS format. 7. Project Beneficiary Groups Beneficiary Groups Capacity Building] Target Achieved No. of Beneficiaries with income generation: nil 40 No. of stakeholders trained, particularly women: - - No. of capacity building Workshops/ trainings: - 01 No. of Awareness & outreach programmes: - 01 No. of Research/ Manpower developed: - -

8. Project Progress Summary* (as applicable to the project)

Description (Name of descriptive Annexure/ File Description Total (Numeric) attached)* IHR States Covered 2 Himachal Pradesh, Uttarakhand Project Site/ Field Stations 2 (attach photos) Survey site (Annexure II) & photos of Genebank and Developed: 3 (attach maps) Survey site (Annexure III) No. of Patents filed - - (Description): Article/ Review/ Research A Kumar, S. Shashni, P. Kumar, D. Pant, Ashok 1 Paper/ Publication: Singh, R.K. Verma. 2021. Phytochemical

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constituents, distribution and traditional usages of Arnebia euchroma: a review. Journal of Ethnopharmacology, 271: 113896 (IF:3.69) https://doi.org/10.1016/j.jep.2021.113896 New Methods/ Modellings Developed (description in 250 - - words): One day training program is conducted at Bathad- Banjar Kullu, organized by GBPNIHE Himachal No. of Trainings (No. of 01 (attach Regional Centre, Mohal-Kullu and CSIR-IHBT Beneficiaries): photos) Palampur under the funded project NMHS-PMU (dated 22 February 2021). (40 farmers) Workshop: - - Demonstration Models (Site): 2 Bathad-Banjar Kullu and Lahaul Livelihood Options: - - Training Manuals: - - Processing Units: - - Collected species from wild like Angelica glauca, Arnebia euchroma and Carum carvi and established Species Collection: 3 field genebank at CeHAB Ribling, CSIR-IHBT, Dauranallah Kullu Recorded diversity of species found with Angelica Species identified: >100 glauca, Arnebia euchroma and Carum carvi populations Database (Numeric/Images/GIS Angelica glauca, Arnebia euchroma and Carum 3 Maps, etc.): carvi *Attach a separate descriptive Annexure/ File. Note: Numeric Database should be provided in .xls format. Photos/Maps should be submitted in high quality (min. 300 dpi res.) compatible formats viz., JPEG, .JPG, .PNG, .SHP, etc. along with suitable figure legend/ caption. 9. Project Linkages (with concerned Institutions/ State Agencies): S. No. Institute/ Organization Type of Linkages Brief Description 1 HP State Forest Department Endorsement Helping the state government for conservation of RETS and their Reintroduction in the wild Note: Attach a separate, descriptive Annexure/ File. 10. Knowledge Products – Publication, recommendations, etc. Time Period Publications (Research Papers, Information Material, Policy drafts, Patents, etc.) Annual [Year 2020 _21] A Kumar, S. Shashni, P. Kumar, D. Pant, Ashok Singh, R.K.Verma.2021. Phytochemical constituents, distribution and traditional usages of Arnebia euchroma: a review. Journal of Ethnopharmacology, 271: 113896 (IF:3.69) https://doi.org/10.1016/j.jep.2021.113896 [Name of descriptive Annexure/ File attached] Note: Attach a separate, descriptive Annexure/ File.

11. Project Concluding Remarks Kindly update the following Progress Parameters for the Reporting Period: Project Quantifiable Progress made against Monitoring Indicators Remarks Objectives Output (specified in Sanction Letter) against each

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objective

1). Identification Angelica glauca: - Morphologic of potential Major sites (10 Nos.): Lahaul Valley (Tindi, Salgran, al studies of natural habitat Kukumseri, Naingar, Dorni, Yangla, Sissu & Salpat); (10 Nos.) of selected wild Uttarakhand (Chamoli); Kangra, Shimla (Rohru) RET Habitats: 04 populations population and No. of accessions surveyed: 10 Nos. and their Number of plants conserved: 1600 collection of morphological Arnebia euchroma: different studies Major sites (13 Nos.): (Jobrang, Sissu waterfall side, Rarik, accessions of Sirla gompa, near Rohtang tunnel, Beeling nallah, Dalang selected maidan, Gwadhi nallah, Thorang nallah, Kibber, Chicham, commerciall Hikkim & Lossar) Habitats: 4 Nos. y important No. of accessions surveyed: 08 Nos. threatened Number of plants conserved: 1050 & 100 gm seeds medicinal Carum carvi: plants Major sites (09 Nos.) Lahual Valley (Khanjhar, Changut, Karpat, Lobber, Khanghsar, Gondla, Purat, Khorpani & Ropsang) Habitats: Agriculture Grassland No. of accessions surveyed: 09 500gm seeds were collected Habitat types identified:11 Shady, Shady Moist, Riverine, Rocky, Dry, Scrub slope, Alpine scrub, Roadside, Sandy, Dense forest, Agriculture field bunds 2. Collection of Field Genebank establishment at CeHAB Ribling, Lahaul- More Establishmen variable Spiti: The area is sown under cultivation 1100 m2, Angelica selection t of a gene accessions, glauca (10 accessions, 1600 plants and 6000 polybags); s of elite bank, field layout plan for Arnebia euchroma (13 accessions, 1050 plants and 2000 accession evaluation, field- polybags); Carum carvi (09 accessions, seeds 1.5 kg) will be chemical and genebank, Characterized and selected elite accessions: Elite accession further molecular plantation, of Arnebia euchroma from ; Carum carvi from done evaluations Sissu & Khangsar villages in Lahaul; Angelica glauca from characterizati and Naingar in Lahaul, area etc. were selected on the basis of their on of target characterizatio physical appearances. species n for selection 3.Mass Target for Planting materials (Seeds/Rhizomes/Roots) of diverse Conserve multiplicatio conservation accessions of Carum carvi, Angelica glauca and Arnebia d plants n of the of 30,000 euchroma were conserved with their proper layout plan in the from the plants this planting field genebanks of CeHAB Ribling, CSIR-IHBT Palampur, GB field year (out of Pant National Institute of Himalayan Environment Mohal Kullu genebank material total 1 Lakh and farmers field, respectively. Also, achieved the conservation will be plants) target of plants of these species for this year. reintrodu The seeds of the Angelica glauca and Carum carvi of ced in different accession has been demonstrated among the farmers their of Upper Manali, Wari villages and Tirthan valley. Also, natural regularly monitored the status of variable accessions of target habitats species and currently established very well. this year. To impart the technological awareness, one day workshop cum training program was also organised and demonstrated in the field.

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Methodology Morphological studies of selected wild populations and collection of different (in brief): accessions of selected commercially important threatened medicinal plants: For the present study, the survey work for the collection of sample of planting material was conducted in the high altitude areas of Western Himalaya, Himachal Pradesh (31°19′– 32°45′N and 76°10′–77°58′E), during the month of July to October, 2020. In their natural populations (30 sites), ecological and morphological studies for Angelica glauca, Arnebia euchroma and Carum carvi were examined by using the quadrate sampling method under different habitats. Due to pandemic situation we have conducted in-depth surveys mainly in the cold desert region of Himachal Pradesh (28 sites), and one site each at Chamoli and Shimla (Annexure I). Mostly in the winter months heavy snow occurred. In the limited working season (about 5 summer months) we have to achieve the target of project site at CeHAB Ribling, Lahaul-Spiti (3450 masl). The climate of the CeHAB office is dry temperate type, in the summer months experiencing a maximum air temperature of 25°C during the day time and minimum freezing temperature at night time. In the winter months air temperature ranges in-between +8 to -200C. Habitat-wise the survey was conducted along an altitudinal range for the target species like Angelica glauca, Arnebia euchroma and Carum carvi. Each surveyed points were geo-referenced with GPS coordinates. Remote sensing based studies have been attempted for the mapping and monitoring of high altitude IHR including alpine zones. The data on the occurrence of individual species in each quadrate were counted. The percentage frequency (% F), density (D plant m-2) and Importance Value Index values were calculated in their natural population. Also, morphological parameters were compared between natural and cultivated accessions. To find out variations, regular morphological studies for the conserved accessions in the field genebank were conducted. Establishment of a gene bank, field evaluation, chemical and molecular characterization of the target species: After observing morphological parameters of each accession in the wild, the collections were carried out to enumerate in the germplasm banks at one location. Time of sowing of the planting material, emergence, establishment rate, harvesting, yield, and package/storage practices were done in the field Genebank of CeHAB farm site at Ribling (Lahaul & Spiti, 3450 masl). Generally, the plants raised from the seeds takes longer time (> 2 years) than vegetative ones to establish. The availability of planting materials (by seeds or vegetative means) were used for the conservation and laboratory analysis. For quick multiplication, higher preference was given to vegetative propagation, so that a large number of planting materials were ensured within a time frame period of the project. Major Research Identified various sites of selected RET plants in the Himachal Pradesh and Achievements: Uttrakhand. The distribution of species like Arnebia euchroma is recorded maximum in Lahaul- Spiti; Angelica glauca in Kullu, Chamba and Lahaul valley; Carum carvi in Lahaul- Spiti district. The GIS map of the selected populations were prepared and submitted. Surveyed the various populations for morphological, ecological parameters and collected different accessions from the Himachal Pradesh and Uttarakhand. Field genebank cum nursery established at different locations for the establishment of the field genebank at CeHAB Ribling (Lahaul-Spiti; at 3450 masl), Dohranallah nursery in GBPNIHE Kullu (1200 masl), CSIR-IHBT Palampur at (1472 masl) and Bathad village at Kullu (2300 masl) prepared. Collected accessions samples of selected RET plants were established in the field genebanks by conventional and tissue culture methods. Accessions of selected RET plants (conserved) were chemically characterized. Brief The number of Medicinal Plants (MPs) multiplied through tissue culture (Nos.): Conclusion - Arnebia euchroma: 450 Nos. NMHS 2020 NMHS-Annual Progress Report (APR) Pro Forma Page 10 of 27

the current year Carum carvi: 1100 Nos. progress – Angelica glauca: 500 is under process during the Initially, standard SoPs were prepared and likely to be mass multiplied reporting The number of Medicinal Plants (MPs) multiplied through conventional method (Nos.) period (point- At CeHAB Ribling, Lahaul-Spiti of last and this year wise): Carum carvi: 20,000 plants Angelica glauca:1,600 plants and seeds sown in 6000 polybags Arnebia euchroma: 1,050 plants and seeds sown in 2000 polybags Progress 60% Achieved (%): Remaining Mass conservation of species: to achieve target given under the project work to be Selection of more elite accessions: after conserving three year crops in the field done: genebanks Reintroductions/restoration of RET species in their natural habitats Ecological / morphological studies of more accessions in their wild populations