(NADSYN1) (NM 018161) Human Tagged ORF Clone Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC200131

NAD Synthetase (NADSYN1) (NM_018161) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: NAD Synthetase (NADSYN1) (NM_018161) Human Tagged ORF Clone Tag: Myc-DDK Symbol: NADSYN1 Synonyms: VCRL3 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 5 NAD Synthetase (NADSYN1) (NM_018161) Human Tagged ORF Clone – RC200131

ORF Nucleotide >RC200131 ORF sequence Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGGGCCGGAAGGTGACCGTGGCCACCTGCGCACTCAACCAGTGGGCCCTGGACTTCGAGGGCAATTTGC AAAGAATTTTAAAGAGTATTGAAATTGCCAAAAACAGAGGAGCAAGATACAGGCTTGGACCAGAGCTGGA AATATGCGGCTACGGATGTTGGGATCATTATTACGAGTCGGACACCCTCTTGCACTCGTTTCAAGTCCTA GCGGCCCTTCTGGAGTCTCCCGTCACTCAGGACATCATCTGCGACGTGGGGATGCCTGTAATGCACCGAA ACGTCCGCTACAACTGCAGAGTGATATTCCTCAACAGGAAGATCCTGCTCATCAGACCCAAGATGGCCTT GGCCAATGAAGGCAACTACCGCGAGCTGCGCTGGTTCACCCCGTGGTCGAGGAGTCGGCACACAGAGGAG TACTTTCTGCCTCGGATGATACAGGACCTGACAAAGCAGGAAACCGTACCCTTCGGAGATGCGGTGCTGG TGACATGGGACACCTGCATTGGAAGTGAGATCTGTGAGGAGCTCTGGACACCCCACAGCCCGCACATCGA CATGGGCCTGGATGGCGTGGAGATCATCACCAACGCCTCGGGCAGCCACCACGTGCTGCGCAAAGCCAAC ACCAGGGTGGATCTCGTGACTATGGTCACCAGCAAGAACGGTGGGATTTACTTGCTGGCCAACCAGAAGG GTTGCGACGGGGACCGCCTGTACTACGACGGCTGTGCCATGATCGCCATGAACGGAAGCGTCTTTGCTCA AGGATCCCAGTTTTCTCTGGATGACGTGGAAGTCCTGACGGCCACGCTGGATCTGGAGGACGTCCGGAGC TACAGGGCGGAGATTTCATCTCGAAACCTGGCGGCCAGCAGGGCGAGCCCCTACCCCAGAGTGAAGGTGG ACTTTGCCCTCTCGTGCCACGAGGACTTGCTGGCACCCATCTCTGAGCCCATCGAGTGGAAATACCACAG CCCTGAGGAGGAGATAAGCCTTGGACCTGCCTGCTGGCTCTGGGATTTTTTAAGACGAAGTCAACAGGCA GGGTTTTTGCTGCCCTTGAGTGGCGGGGTGGACAGCGCAGCCACCGCCTGCCTCATCTACTCCATGTGCT GCCAGGTCTGCGAGGCCGTGAGGAGTGGAAATGAGGAAGTGCTGGCTGATGTCCGCACCATCGTGAACCA GATCAGCTACACCCCCCAGGATCCCCGAGACCTCTGTGGACGCATACTGACCACCTGCTACATGGCCAGC AAGAACTCCTCCCAGGAGACGTGCACCCGGGCCAGAGAGTTGGCCCAGCAGATTGGAAGCCACCACATCA GTCTCAACATCGATCCAGCCGTGAAGGCCGTCATGGGCATCTTCAGCCTGGTGACGGGGAAGAGCCCTCT GTTTGCAGCTCATGGAGGAAGCAGCAGGGAAAACCTGGCGCTGCAAAATGTGCAGGCTCGAATACGGATG GTCCTCGCCTATCTGTTTGCTCAGTTGAGCCTCTGGTCTCGGGGTGTCCACGGTGGGCTCCTCGTGCTGG GATCCGCCAACGTGGATGAGAGTCTCCTGGGCTACCTGACCAAGTACGACTGCTCCAGTGCGGACATCAA CCCCATAGGCGGGATCAGCAAGACGGACCTCAGGGCCTTCGTCCAGTTCTGCATCCAGCGCTTCCAGCTT CCTGCCCTGCAGAGCATCCTGTTGGCGCCGGCCACCGCAGAGCTGGAGCCCTTGGCTGATGGACAGGTGT CCCAGACCGACGAGGAAGATATGGGGATGACATATGCGGAGCTCTCGGTCTATGGGAAACTCAGGAAGGT GGCCAAGATGGGGCCCTACAGCATGTTCTGCAAACTCCTCGGCATGTGGAGACACATCTGCACCCCGAGA CAGGTCGCTGACAAAGTGAAGCGGTTTTTCTCCAAGTACTCCATGAACAGACACAAGATGACCACGCTCA CACCCGCGTACCACGCCGAGAACTACAGCCCTGAGGACAACAGGTTTGATCTGCGACCATTTCTGTACAA CACAAGCTGGCCTTGGCAGTTTCGGTGCATAGAAAATCAGGTGCTACAGCTCGAGAGGGCAGAGCCACAG TCCCTGGACGGCGTGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 5 NAD Synthetase (NADSYN1) (NM_018161) Human Tagged ORF Clone – RC200131

Protein Sequence: >RC200131 protein sequence Red=Cloning site Green=Tags(s)

MGRKVTVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVL AALLESPVTQDIICDVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSRHTEE YFLPRMIQDLTKQETVPFGDAVLVTWDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHHVLRKAN TRVDLVTMVTSKNGGIYLLANQKGCDGDRLYYDGCAMIAMNGSVFAQGSQFSLDDVEVLTATLDLEDVRS YRAEISSRNLAASRASPYPRVKVDFALSCHEDLLAPISEPIEWKYHSPEEEISLGPACWLWDFLRRSQQA GFLLPLSGGVDSAATACLIYSMCCQVCEAVRSGNEEVLADVRTIVNQISYTPQDPRDLCGRILTTCYMAS KNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPLFAAHGGSSRENLALQNVQARIRM VLAYLFAQLSLWSRGVHGGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCIQRFQL PALQSILLAPATAELEPLADGQVSQTDEEDMGMTYAELSVYGKLRKVAKMGPYSMFCKLLGMWRHICTPR QVADKVKRFFSKYSMNRHKMTTLTPAYHAENYSPEDNRFDLRPFLYNTSWPWQFRCIENQVLQLERAEPQ SLDGVD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV Chromatograms: https://cdn.origene.com/chromatograms/mk6291_c09.zip Restriction Sites: SgfI-MluI Cloning Scheme:

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 5 NAD Synthetase (NADSYN1) (NM_018161) Human Tagged ORF Clone – RC200131

Plasmid Map:

ACCN: NM_018161 ORF Size: 2118 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_018161.3 RefSeq Size: 2453 bp RefSeq ORF: 2121 bp Locus ID: 55191 UniProt ID: Q6IA69 Domains: CN_hydrolase, NAD_synthase Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism MW: 79.3 kDa Gene Summary: Nicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD synthetase (EC 6.3.5.1) catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD).[supplied by OMIM, Apr 2004]

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 4 / 5 NAD Synthetase (NADSYN1) (NM_018161) Human Tagged ORF Clone – RC200131

Product images:

Western blot validation of overexpression lysate (Cat# [LY413259]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un- transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200131 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).

Coomassie blue staining of purified NADSYN1 protein (Cat# [TP300131]). The protein was produced from HEK293T cells transfected with NADSYN1 cDNA clone (Cat# RC200131) using MegaTran 2.0 (Cat# [TT210002]).