N B A G R N B A jkG iR vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G Rokf"kZd izfrosnujk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R N B A Gjk iR vk la C;wjks N B A jkG ANNUALiR vk la C;wjks REPORT jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR 2017vk la C;wjks - 18 N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A Gjk iR vk la C;wjks N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A jkG iR vk la C;wjks N B A Gjk iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A G R jk i vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A G R jk i vk la C;wjks N B A G R N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R jk i vk la C;wjks jk i vk la C;wjks jk-i-vk-la-C;wjksN B A G R N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R N B A jkG iR vk la C;wjks N B A Gjk iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R N B A jkG iR vk la C;wjks N B A Gjk iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks jk i vk la C;wjks N B A G R N B A Gjk iR vk la C;wjks N B A Gjk iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks jk i vk la C;wjks N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R HkkÑvuqi&jk"Vªh; i'kqjk vkuqoaf'kdi vk la C;wjks lalk/ku C;wjks N B A G R jk i vk la C;wjks N B A G R djuky & 132001 (gfj;k.kk) HkkjrN B A jkG iR vk la C;wjks N B A Gjk iR vk la C;wjks jk i vk la C;wjks N B A G R jk i vk la C;wjks ICAR-National NBureau B A of AnimalG R Genetic Resources Karnal - 132001 (Haryana) India N B A jkG iR vk la C;wjks N B A Gjk iR vk la C;wjks jk i vk la C;wjks N B A G R jk i vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R jk i vk la C;wjks N B A G R ii ANNUAL REPORT 2017-18 343, 1 Intech Printers&Publishers Printing Ms InduBala,StenoGr.III Dr PSDangi,A.C.T.O. Assistance I/C, P&EUnit Photographs Dr SonikaAhlawat,Scientist Dr JayakumarS,Scientist Dr SaketKumarNiranjan,Sr.Scientist Dr AKMishra,Pr.Scientist Dr RSKataria,Pr.Scientist Dr NKVerma,Pr.Scientist&I/c,PMECell Editors Dr JayakumarS,Scientist Dr SonikaAhlawat,Scientist Dr NKVerma,Pr.Scientist&I/c,PMECell Compiled by Karnal -132001(Haryana)India ICAR-National BureauofAnimalGeneticResources Dr ArjavaSharma,Director Published by Line... Credit E-mail: [email protected] Tel: 0184-4043541,3292951 Karnal -132001,Haryana jk-i-vk-la-C;wjks st Floor,MughalCanalMarket, NBAGR

m

Research Projects and Publications Research Accomplishments History and Profile Summary Executive From Director's Desk fgUnh [k.M Personnel Other Activities Capacity Building Network Project on AnGR &EvaluationGenomics of AnGR CharacterizationGenetic of AnGR of AnGR Conservation IdentificationCharacterization & of AnGR InformationLivestock Management Financial Outlay 7 Organogram ProfileDivisions' About Bureau jktHkk"kk izdks"Bdhxfrfof/k;k¡ dk;Zdkjh lkjka'k funs'kd dhdyels--- Superannuation Joining/Transfers Promotions Personalia 77 Distinguished Visitors Celebrations 72 Important Meetings Library Mera Gaon Mera Gaurav Exhibitions Trainings Attended Trainings Organized Awards Publications Research Projects

79 Contents

89 85 83 47 29 27 25 15 13 79 79 74 71 71 67 67 66 65 60 53 51 iii 8 4 3 i genes and pathways underlying meat quality meat underlying pathways and genes Attempts were also made to identify candidate buffaloes. and cattle indigenous our to value add will hope I which generated being also has profile genotypic and allelic A1A2 breeds. populations/ goat and sheep buffalo, cattle, of characterization genetic as well as phenotypic addition, some more projects were initiated on Ladakh was carried out through field surveys. In of population Yak and Karnataka of goats Rajasthan, Dumba sheep, Bidri and Nandidurga buffalo of Odisha, Chitarangi sheep of Punjab and Manda Nadu, Tamil and Kerala of population cattle non-descript of characterization genotypic and phenotypic year, the During been presentedthroughthisdocument. achievements made during the year 2017-18 has 18 chicken breeds so far. A brief report of overall camel, 7 pig, 1 yak, 1 duck, 1 geese, 1 donkey and buffalo, 28 goat, 42 sheep, 7 horse and ponies,9 13 cattle, 41 registered and characterized have we 1984, since excellence scientific of journey glorious the During registration. their and utilization conservation, evaluation, catering to breed identification, characterization, country the of institute only the is NBAGR ICAR- resources. genetic animal farm Indian of potentials genetic the manage and identify to working continuously is institute the 1984, September 21 on establishment its Since management during2017-18. technology and education extension, training, research, of areas the in made achievements the of glimpses has which Report Annual the present to pleasure feel I repute, international A From Director'sDesk... peir nttt o ntoa and national of Institute premier a Karnal, NBAGR ICAR- of Director the s NBAGR house discussions. Bureau is currently running currently is Bureau discussions. house in- through improved and screened also were of completed projects. New proposals proposed report final the approve and projects on-going of progress the review to time on held were meetings (IRC) Committee Research Institute agencies ofthecountry. various to males breeding of deficiency) XI Factor and Citrullinemia DUMPS, (BLAD, testing disease and screening cytogenetic of services consultancy providing also is Institute registration. breed for eligible them make and populations new of identification in help will efforts These characterization. for selected been have breeds/populations new 10 AnGR, on program Network the Under were added. breeds cattle Nagori and Khillar Rathi, Dangi, of doses semen NBAGR, at Bank Gene National the In preserved. and generated were camel fibroblast cell lines of Manipuri horse and Kutchi 41 Hariana) were born. For progress and a total of 46 calves (5 Sahiwal and in is Gaushalas through cattle Hariana and Sahiwal of conservation which under NBAGR of mandate important an is Conservation altitude adaptedZanskarponies. high in trait endurance and sheep Mandya in ex- situ ex- conservation, jk-i-vk-la-C;wjks

i ANNUAL REPORT 2017-18 fROM dIRECTOR'S dESK ... ii ANNUAL REPORT 2017-18 fROM dIRECTOR'S dESK ... our work. Foundation Day of Bureau, Biodiversity Dr. J K Jena, visited the bureau and appreciated (DARE) & DG, ICAR and Deputy Director General Secretary Mahapatra, T Dr. resources. genetic farmers about the benefits of indigenous animal sensitize and activities institute’s showcase to places various at organized were AnGR on exhibitions Five Abhiyan. Swachhta Rashtriya Mera Gaon Mera Gaurav, Jai Kisan Jai Vigyan and like programmes national the of some support Scientists of the Bureau participated actively to presented theirwork. and participated they where fora scientific various at recognised was scientists bureau of expertise The departments. husbandry animal of officials and veterinarians field teachers, workers, research for programs training five organizing by building capacity to contributed also has institute The period. reported the during published also were chapters book four twenty and monographs five books, Five factor. impact high of journals international popular articles were published in national and technical/ 12 and papers research 33 of total A project. Fellow National one and funded externally five including projects research 22 jk-i-vk-la-C;wjks NBAGR fROM dIRECTOR'S dESK... for ourfurtherimprovement. criticism and suggestions the welcome always achievements in the form of this document. We editorial committee which shaped the Bureau’s the thank to like also would I time, same the contribution in the progress of the institute. At staff members of the institute for their fabulous other and administrative technical, scientists, the all congratulate I satisfaction, and pride of sense great a With acknowledged. gratefully is Committee Advisory Research from received overall development of this Institute. Guidance the for support their for Delhi New ICAR, Sci., S Gandhi, ADG (AP&B) and Dr Vineet Bhasin, Pr. R Dr (AS), DDG Jena, K J Dr. ICAR, DG, & (DARE) Secretary Mahapatra, T Dr. to grateful am I registered livestockbreeds. indigenous rearing and keeping in involved organization and institutes people, for awards" first time NBAGR has started "Breed conservation the For enthusiasm. full with campus NBAGR at celebrated were Pakhwada Hindi Week, day, Republic Day, Independence Day, Vigilance (ARJAVA SHARMA) DIRECTOR based informationbased on activities of animal Punjab was initiated year. this Questionnaire for management the of AnGR inHaryana and evaluating livestock and policies programmes Under assigned newly mandate, the study for reached 169. indigenous livestock breeds and have poultry duckyak, and geese. Total number of registered firsttime registeredthe indigenous breeds of from NE region of country. the The institute has registered by ICAR-NBAGR. Five breeds were of horse, pig, duck geese, yak, and chicken – were – one ofpoultry cattle, two of goat, and one each Nine breeds new of indigenous livestock and during 2017-18through its different activities. Bureau could raise revenue the of Rs.35.66Lakhs againstRs. 76.6 Lakhs, Receipt of Rs. 77.0 Lakhs. Under Network project, total expenditure was during financial1335.3 Lakhs year of 2017-18. againstRs. 1318.49Lakhs total the receipt of Rs. Revenue and Pension &retirement benefits) was Total expenditure under different (Capital,heads Institute’sthe activities. Network Project are functioning to also support Livestock Information & Management and mandate. In addition, two units namely- createdbeen in2013to achieve institute’s the andGenetics, 3)Animal Biotechnology have Resources- 1)Animal Genetic 2)Animal management issues. and Three policy Divisions and capacity building inanimal genetic resources resources and 2)coordination of country; the utilization of livestock genetic and poultry evaluation, characterization, conservation and It has abroader mandate of 1)Identification, livestock breeds, country. and inthe poultry for benefit the and upliftment of indigenous instituteResources nodal the has been working ICAR-National Bureau of Animal Genetic EXECUTIVE SUMMARY NBAGR fibre transport.and Yaks of Nubra are medium community located for meat, milk, manure, hair characterized. are They reared by Buddhist Jammu & Kashmir, population yak local was inNubra region survey During of (Ladakh) reared for meat only. purpose are Black. Ears were pendulous. goats Both were goats, coat colour, muzzle, eyelids and hooves or black. Ears are and pendulous. leafy In Bidri Nandidurga is white, whereas, eyelids are brown Bidar and Kalaburagi of districts Karnataka. and Davangere, whereas Bidri is distributed in goats are distributed inChitradurga, Tumkur goats of Karnataka was completed. Nandidurga Characterization of Nandidurga and Bidri grouping by discriminant analysis. traits of Odisha could buffaloes distinct reveal is causetract of concern. Various biometric population of Sambalpuri in native buffaloes adjoining of districts Chhattisgarh. Dwindling is found inSambalpur of district Odisha and ranges from 2to 4kg/day. Sambalpuri buffalo for ploughing inhard laterite soil. yield Milk typical shape. These are excellent draught animal blackish-brown. Horns are long and heavy with andsmall compact body. colour Body is light Koraput of district Odisha. Manda possesses in Odisha state. Manda are distributed in Manda and Sambhalpuri buffaloes were surveyed overbest other the methods. and 1-to-Rest, the SVM performed model the In(SVM) methods. partition binary the 1-to-1 networks (ANN)and support vector machine analysis (DFA), and neural heuristic - artificial traits using classical -discriminant functional India were seven classified morphometric based on Eight indigenous sheep breeds/populations of keepers and other stakeholders. fromhusbandry was collected Farmers/livestock jk-i-vk-la-C;wjks

iii ANNUAL REPORT 2017-18 EXECUTIVE SUMMARY iv ANNUAL REPORT 2017-18 EXECUTIVE SUMMARY milk yield rangesmilk from 1.0 to Jhari 2.5kg. cattle areBullocks for used work. agricultural Daily long and concave. Horns are long and curved. of Warangal is white district colour. Face is and Kamma)Zari of Telangana state. Vandarvi of known lesser cattle populations (Vandharvi, wereSurveys conducted for characterization It is mainly reared for rituals (Qurbani). characterized by deposition the of fat tail. inthe large in size, pendulous and drooping. Dumba is colour is white and brown inmajority. Ears are Nagaur and Ajmer of districts Rajasthan. Coat and characterization. It is found mainly in Jaipur, was conducted for Dumba sheep distribution wool production is 1.5to Similarly, 2kg. survey enddistal of ear pinna. Average annual greasy of different shape and depth are available on around eyes, the muzzle and on ear. the Serrations large insize. colour Coat is white with tan patches sheep population.type Chitarangi is medium to Nagar of district Rajasthan. It wool is acarpet in Fazilka, Muktsar of districts Punjab, Ganga Sri Chitarangi and sheep was characterized surveyed decline human concern. has largely due declined to fading utility and to track innight easily also. Donkey population or white belly. Animals are sure footed and able dark grey, brown and black with light coloured and colour Coat sized docile. varies from light to transportation and manure. Animals are medium donkey is reared by Buddhist communities for region ofLadakh Jammu & Kashmir. Ladakhi Native donkey population was characterized in cause of decline of population. yak was evident region inthe and was asignificant under pastoralism. Preference of cross-hybrids thanwider shoulder region. Most are yaks reared upward and backward. Ears are Pelvis small. is black. Horns and laterally areplaced curved and moderate. Coatcolour is dark brown to jk-i-vk-la-C;wjks NBAGR EXECUTIVE SUMMARY both populations.both Any large or recent genetic sufficient the confirmed level of diversity in found heterozygosity polymorphic. Observed Bidri goats of Karnataka, markers the all were geneticDuring analysis Nandidurga and Two camps health were organized. also whereas inHariana ranged from 1.0to 4.0kg. yield rangeddaily milk from 4.0to 9.0kg, purity. Total 46calves were In born. Sahiwal, wereSahiwal inseminated with maintain breed cows.Sahiwal Atotal of 52of Hariana and 4of improvement and conservation of Hariana and of Haryana were for selected genetic the of indigenous cows inGuashalas. Three Gaushalas sustainable improvement vis-à-vis conservation Under project the for developing of amodel from 3rd to passage. 6th cryopreserved The media. cellsspecific were using explants culture technique using fibroblast culture culture method. Primary was established for establishment of lines cell by attachment cell Manipuri horse and Kutchi camel were utilized somatic cell banking. marginal Ear tissues of linescell were generated from skinexplants for Manipuri Horse and Kutchi camel fibroblast for cryopreservation. of Katchi camel and Marwarihorse were added 8 cattle breeds and 400somatic each doses cell and somatic cells). Total of doses 23975 semen DNA, embryos, (semen, epididymal sperms by diversified preserving form of germplasm ICAR-NBAGRBank, is strengthened being Germplasm repository at National Gene in cows. cattle. Hump is moderate and inbullocks small differentiation.than OngoleBody is smaller shape. Long faceis major the character for are grey in majority. and is small cylindrical Body markers. The mean F respectively were identified across 24 the and Dumbasheep, 237and 243distinct alleles, In order to genetically characterize Chitarangi haplotypesspecific also. had moreChilika buffaloes. number of breed of haplotypes and swamp Chilika between Median joining network indicated sharing maximum admixture of Kalahandi buffalo. mostbeing distinct. Paralakhemundi had Structure analysis Manda revealed and Murrah divergedwas highly from other populations. all populationsOdisha buffalo indicatedthat Manda Microsatellite markers data analysis based of five showed populations both as distinct. bottleneck was absent. 2D coordinate analysis was significantly in HF, higher Sahiwal and good expressionthe of DAZL Boule, and BvH genes motility.sperm To effect, assess functional the and PIWIL1 genes of Frieswal bulls with poor DNA methylation in DAZL, was observed BvH HF crossbred (Frieswal) Higher was assessed. genesspecific in spermatozoa of Sahiwal, HF and cattle fertility, methylation the profile of meiosis To speculate role the of methylation in crossbred were relevant to meat quality. functions. Prominent pathways that detected components and 117 terms for molecular for biological process, 146 terms for cellular classified intoGene Ontology 132 (GO) terms differentiallythe expressed genes could be regulated and 602up regulated inBandur. All Among 1236 these, were significantly down total of 20568 differentially expressedtranscripts. Bandur sheep of and Karnataka local a revealed Transcriptomic profiles of skeletal muscles of numberthe of heterozygotes. two populations,these indicating in deficiency inbreeding estimates) was 0.096and 0.043in EXECUTIVE SUMMARY NBAGR IS (within population samples were for analysis. collected further mice (diabetes progression) was completed and BCM9 inBCM7 effect the toand ascertain of ofmilk Karan out. Fries carried was also Trials cattle Expression types. analysis in casein of beta variant across arg) (ser122 three the was observed 13 known variants, other than A1/A2 only B native, exotic and crossbred cattle. Amongst the variants was estimated casein of beta across Indian andAllelic genotypic profile known forthirteen Red andRed least inMarwari. of HSPs was found highest the inMadras to be concentration sheep. inthese expression Gene found significantly affecting the T3 hormone for heat tolerance. at Genotypes were 2 loci SNPs were advantage found selective to carry genotypingDuring for loci of 5selected under normalassessed and stressed condition. Magra, Marwari, and Madras sheep was Red Functional profile of HSPs genes inChokla, during exercise. differentiallyto be Zanskar expressed in ponies altitude.high Atotal of 646genes were found genes before and a generated to differentially identify expressed during endurance exercise at altitude high was Transcriptome analysis of Zanskar PBMCs distinct. populationsand yak Sikkimi were found to be qualityhigh with coverage of 9.42%.Ladakhi respectively. 579575SNPs Overall, were of RD10 were 256051,241934,312518and 150425, and Total Sikkimi. Ladakhi SNPs identified at in four populations yak -Arunachali, Himachali, Genome SNPs wide and INDELs were identified motilitybulls semen with poor as control. motility crossbred bulls as compared to Frieswal ­fter endurance exercise at jk-i-vk-la-C;wjks

v ANNUAL REPORT 2017-18 EXECUTIVE SUMMARY vi ANNUAL REPORT 2017-18 EXECUTIVE SUMMARY lactation. regulation (II vs I) during different stages of Pathway (IIvsI)and exercise induced circardian Cardiacthe ((IIIvsIIstage), BDNF Signalling pathway (IIIvsIstage), calcium regulation in of up regulated genes were MAPK signalling lactation stages of buffaloes. Majorpathways transcriptomeMilk was generated for different traitthe estimated. can be availability of SNPthe effects, predicted value of on SNPbased effects were estimated.also After yield trait. milk the BLUP and BLUE of trait the genomic region had astrong association with Manhattan plot suggested that surrounding the traitsmilk of 1763daughters of 12sires. the The out on SNP based markers and phenomic data on Genome Wide Association Studies was carried eleven groups, largely by region-wise. allowed grouping the ofpopulations the all into Assam and Manipuri. Spatial genetic analysis of except country Mehsana, the Chilika, Toda, admixture for most ofpopulations buffalo the breeds/populations. There degree was high of identification of admixture among 17 buffalo Total 23,306 SNPs were analysed for association mapping. buffaloes werebe determined to required for an - 1,50,000for riverine and 1,20,000for swamp -HH3, HH4and Holstein cholesterol deficiency Jersey Haplotype Holstein (JH1), haplotypes of differentLoci including lethal genetic diseases transcripts out. carried was also assuming r buffaloes and 25-50 kb forthe swamp buffaloes, distance block of 20-25kb was found for riverine Total 23,306SNPs were genotyped. Genetic toutilized estimate linkage disequilibrium. populationsin 17buffalo (625 animals) was Genotyping by Sequencing (GBS) data generated jk-i-vk-la-C;wjks De novo De 2 greater than 0.2.Number of SNPs assembly of buffalo the NBAGR EXECUTIVE SUMMARY the benefitsthe of indigenous animals. Institute also institute’s and activities sensitize farmers about were organized at various places to showcase doorsteps andSeven exhibitions field. on AnGR and created awareness among farmers on their Gaon Mera Gaurav and visited various villages Scientists of Bureau the participated inMera of ICAR institutes. administrative, and technical supporting staff were organized for upscaling of skill the and staff. academic Twotraining programmes Training was conducted Course for scientific One eachtraining of Short and Course Model monographs were published also by institute. the articles were published. Five each of and books impact factor. Twelve technical/popular National and International Journals of high Total 33 research papers were published in Fellow projects are institute. inthe running including five funded externally and one National during last year. At present 22 research projects A total of 7research projects were completed 2 breeds are habitat. intheir conserved being have for undertaken characterization been and Under Network project, populations 9new Rs. 8,30,500/-was generated as revenue. bull was found chromosomal the to carry defect. 142 bulls were for screened One genetic diseases. weremales for screened chromosomal defects. agencies. 344bulls/ reported the During period, to different the testing government services Institute provides also genetic the defect/disease JH1 and HH3incattle. protocolsbased were developed for screening of and Holstein genetic groups, respectively. PCR heterozygous condition were identified in Jersey population. Two mutant and alleles-JH1 HH4in (HCD) were characterized inIndian cattle Republic day. Sport Meet-2018 organized on occasion the of participated various inthe events of Annual reached up to semi-final round. Bureau staff andBasket Volley Ball Smashing Ball teams participated ICAR inthe (North Zone) games. A contingent of 22staff members of NBAGR projects was reviewed. were held on and time progress the of research Research Advisory Committee (RAC) meetings The InstituteCommitteeResearch (IRC) and were subscribed. includingjournals 23 foreign and Indian journals developmentsscientific/technical different In Bureau the library, to keep track of current the Day, Hindi Pakhwada inits premises. International yoga day, Agricultural Education Rashtriya Swachhta Abhiyan, Vigilance Week, and Independence Day, Jai Kisan Jai Vigyan, Day of Bureau, Biodiversity day, Republic Day celebrated differentoccasions like Foundation EXECUTIVE SUMMARY NBAGR   ICAR-NDRI for masters and PhD degrees. different institutes and universities, including scientists are regularly guiding students the of involved in teaching various courses. The National Research Institute Dairy were actively disciplinesand Animal Biochemistry to ICAR- &Breeding,Genetics Animal Biotechnology NBAGR scientists of PGfaculty the being Animal symposia and conferences. andwithin country the attended workshops, the Scientists were deputed for training programmes ICARSciences), visited institute. the Deputy Director (Fisheries & Animal General DARESecretary &Director ICAR, General A number of distinguished persons including transferred and one superannuated during year. the grade. Twohigher members staff joined, two stafftechnical gottheir promotionsthe to next working at Institute. the Two scientists and four administrative persons staff and are 4 skilled At present 16technical, 29scientific, 17 jk-i-vk-la-C;wjks

vii ANNUAL REPORT 2017-18 EXECUTIVE SUMMARY viii ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks NBAGR History andProfile FinancialOutlay • Organogram • Divisions'Profile • AboutBureau • NBAGR jk-i-vk-la-C;wjks

1 ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks NBAGR

2 Plan. 5 During T was accepted inprinciple during 4 and conservation of Indian indigenous livestock institutes at started Regional Station of National were up set on 21st September, 1984.These National Institute of (NIAG) Animal Genetics of Resources Genetic Animal (NBAGR) and institutetwin form inthe of National Bureau establishmentthe of institute. this Therefore, a government agencies coordinated efforts the for About Bureau HISTORY ANDPRO FILE y y y y y y y y y y y y animal genetic resources. To conduct training programmes as related to evaluation, characterization and utilization of biology. To studies undertake on genetic characterization techniques using modern of molecular utilization of farm animal genetic resources. To for methodologies design ex-situ conservation and in-situ management and optimal genetic resourcespoultry and to establish National their Data Bank. T Coordination and capacity building inanimal genetic resources management issues. and policy genetic resourcespoultry of country. the Identification,evaluation, characterization, conservation and utilization of livestock and which canwhich after look the characterization forhe need establishment the of an institute o conduct systematic to characterize, evaluate surveys and catalogue farm livestock and NBAGR th and 6 and th Five Year Plan, various th Five Year OBJECTIVES MANDATE objectives as given below: organization inIndia mandate with the and Resources nodal the Animal Genetic has been Resources.Animal Genetic National Bureau of asfunction asingle unit as National Bureau of Instituteboth and Bureau the were merged to came on recommendation the of QRT when Karnal in1994.The unification of two institutes before shifting to its campus own at Makrampur, and temporarily housed inNDRI main campus institutethe were shifted then to Karnal in 1985 Research Institute,Dairy Bangalore. Bureau and jk-i-vk-la-C;wjks

3 ANNUAL REPORT 2017-18 About bureau 4 ANNUAL REPORT 2017-18 Divisions' profile breeds/populations are documented form inthe climatic conditions of habitat. their The surveyed breeds is evaluated and recorded under agro- products. The productionperformance of local performance, and marketing of live animals and biometry,body reproduction and production status, phenotypic practices, characteristics, to assess flocks/herdsurveys populationstructure, accomplished through systematic/pilot field in phenotypic characterization, is which along with its ‘National Bank’ Gene is engaged At present Resources Animal Genetic Division division continues inits present form. ResourcesGenetic Division and since the then Reproduction was merged into Section Animal In Physiology Animal 1996,the Section. and c) Animal Physiology andSection Reproduction ResourcesAnimal Genetic and Conservation and Management Unit/Computer Unit b) and units were again reframed as: a) Information Unit. Bank Gene sections 1994-95the During Resources Unit and and Animal Conservation and Management Animal Genetics Section, and two units Livestock viz. Information NBAGR then 1992-93 the had one section 1991-92. Reorganization continued and in Physiology and Reproduction during section was created was which reframed as Animal In of section 1991 a new Animal Bank Gene Resources Genetics and Section. Conservation divisionsConservation were merged into Animal In Resources 1987,Animal Genetic and Animal into existence after a number transformations.of ResourcesAnimal Genetic (AGR) division came description The methodologies. present form of strategiescharacteristics, and survey breed engagedbeing indeveloping formats for breed ResourceAnimal Genetic (AGR) Division is Resource Division Animal Genetic Divisions' Profile jk-i-vk-la-C;wjks NBAGR HISTORY ANDPRO FILE long term ex-situ conservation of germplasm. division working is also frontier inthe areas of of various livestock In species. addition, the conservation implemented has been for breeds of breeds the under field conditions. situ The in formulated for improvement and conservation on information, the strategies new have been charts inaddition to research publications. Based of breed monographs, breed descriptors and in reputed journals of impact high factor. The research findings have published been breeding bulls for any inheritable abnormality. provide for consultancies/services testing the of important the of activities division the is to division. inthe Genomics running is also One AGRI Research Consortia Platform project on in progress. In addition to one this, ICAR- Four IRC approved research projects are one DBT research funded projects successfully. from division this completed three IRC and under report, scientists period the During supportingskilled staff. one officer, technical one gradeIIIsteno and one three senior scientists, one scientist Scale, Senior division has presently five scientists,principal of Division. part the Animal Genetics The immunogenetics, and molecular genetics became Scientists working fields inthe cytogenetics, of candidate gene analysis of livestock species’. biochemical, cytogenetic characterization and objectivethe ‘Molecular, immunological, Indian of Council Agricultural Research with and was formally approved in2014by the Division was established year inthe 1996 ResourcesGenetic initially. Animal Genetics established with National Bureau of Animal (NIAG)Animal Genetics was which co- transformed form of National Institute of The present Division Genetics Animal is a Division Animal Genetics

casein milk protein milk casein variants on During health. in order to confirmthe role of A1 beta and A2 through proteomic and transcriptomic studies diet and enormous data is generated being as feedingmilk casein A1 and beta A2 type as peptides wellby and BCM9 injecting BCM7 proteins,milk have conducted micetrials been funded, NASF project on casein A1A2 beta indicus cattle and buffalo. In another externally molecular basis ofthe adaptation thermal in transcripts and polymorphic to understand loci made throughbeen identification of unique adaptation, remarkable achievements have Nationalthe Fellow project on heat stress division.in the last During one year, under institute projects or running recently completed collaborativeexternally funded as well as four adaptation traits. At present there are two with various production/disease resistance/ evaluation of genes and transcripts associated of genomic tools for identification the and arespecies with running emphasis on utilization projects on genetic characterization of livestock Variousactivities. institute and externally funded are working areas inthe of mandated institutional AnGR utilization’.scientists The the divisionof genes/biomoleculesfunctional for enhancing division has objective the of ‘Evaluation of andtechnical one supporting skilled staff. The divisionthe has astrength of sixscientists, three DNA erstwhile the Fingerprinting Unit. Currently in 2014,by orders the of council the reorganizing Animal Biotechnology Division was established Division Animal Biotechnology students. in teaching and and guiding M.Sc Ph.D the of part the being NDRI were faculty involved presentations.their Apart from scientists this, international conferences and fetched awards for participatedScientists also innational and HISTORY ANDPRO FILE NBAGR division topped list the again. internationalin the journals of repute, high the fora. Through publishing quality researchpapers posterand presentation several awards at various publication in Journal of Livestock Biodiversity have received SOCDAB young scientist, best Studentsactivities. and scientists of division the involvedalso inteaching and student guiding NDRI, Karnal, scientists of division the are faculty in Animal the BiotechnologyBeing at understand genetic the basis of tick resistance. crossbred cattle is generating also data new to breed. Work of out carried being in indicus and registered to be as new deserving buffaloes, Manda aunique being population among Odisha mitochondrial D-loop sequencing indicates Microsatellitebuffaloes. diversity allelic and and swamp have identified been among Chilika of buffaloes Odishaof state, hybrids riverineof While working on genetic the characterization thawpost motility sperm infrozen semen. important the couldwhich be reason for poor of crossbred, purebred indicus and exotic cattle, methylated CpG sites inBoule to document variations the number inthe of in cattle, scientists of division the have able been understanding molecular basis the of subfertility working on another institutional project on transport in Bandur mutton sheep. While and ion muscle tenderness, fat metabolism and pathways associated with expressed genes sheep, helping inidentification of differentially last one year out work carried in been has also to institute. the looking also is aftersection The provides internet LAN, and computing facilities in an retrievable easily format. also section This on animal resources and to provide it to users the This Unit is engaged in digitization of information Unit InformationLivestock Management gene promoter jk-i-vk-la-C;wjks

5 ANNUAL REPORT 2017-18 Divisions' profile 6 ANNUAL REPORT 2017-18 Divisions' profile core lab was established inNEHregion and were initiated also from IXplan. In XIplan one characterization and conservation activities for characterization of breeds. Genetic centres12 new in each plan were undertaken characterization of breeds. In IXand X plan There were 8 centres in VII plan for y y y followingthe objectives: The Network project was initiatedwith in1996 Network Project Unit resources. registration of livestock genetic and poultry y y y jk-i-vk-la-C;wjks To germplasm the conserve breedsTo develop breed the descriptors candidate gene studies inindigenous Molecular genetic characterization and qualitative and quantitative traits To characterize breeds the interms of both NBAGR HISTORY ANDPRO FILE  (Beetal, Surti) and Surti) Spiti(Beetal, horse. (Magra, Nilgiri, Kilakarsel), 2goat breeds (Pandharpuri, Jaffarabadi, 3 sheep Toda), breeds Kherigarh, Kangayam, Nagori), 3 buffalo breeds includes 5 cattle breeds (Krishna Valley, Ponwar, Mithun.Arunchali of Conservation AnGR Ankleshwar), Kutchi Camel, Spiti horse and Ganjam, Mehsana), 2 breeds of chicken (Aseel, breeds of goat (Osmanabadi, Barbari, Attapady, Banpala, Coimbatore, Chhota-Nagpuri), 5 Deccani, Mecheri, Mandya, Rampur Bushair, Nagpuri, 8breeds Surti), of sheep (Changthangi, Gangatiri), 3breeds of (Jaffarabadi, buffalo Dangi Amritmahal. Khillar, Gaolao, Tho Tho, cattle (Deoni, Ongole, Gir, Umblachery, Bachaur, development of breed descriptors of 11 breeds of phenotypic and genetic characterization and conservation. The updated achievements include work on 4at risk breeds started for has been have for undertaken characterization been and current XII plan breeds/populations 17 new buffalo genomics worktaken also was up. During HISTORY ANDPRO FILE

Organogram NBAGR jk-i-vk-la-C;wjks

7 ANNUAL REPORT 2017-18 ORGANOGRAM 8 ANNUAL REPORT 2017-18 FINANCIAL OUTLAY Financial Outlay *Includes of Releases Rs.68.50lakhs S. N. 7. 6. 5. 4. 3. 2. 1. S. N. 1. 2. 3. jk-i-vk-la-C;wjks HEAD Total Capital capitalii) Other expenditure i) Works Capital Total Revenue v) Miscellaneous expenses iv) Administrative Expenses iii) Research &Operational expenses ii) Traveling Allowance i) Establishment expenses Revenue Grant TotalGrant Pension &Retirement benefits Total Misc.Others Revenue Receipts ofSale farm Produce ofSale Technology Hostel and Guest house rent Training Programs -Income feeLicence ofSale Publication &Advertisement Head of Account Budget Estimate Grants under &Network Project of NBAGR for NBAGR Revenue Generated during theyear 2017-2018 financial year 2017-18 alongwith 2017-18 financial year expenditure Target :35.66Lakhs Achieved RE Grants 43.80 28.00 15.80 1239.00 10.00 185.00 157.00 10.00 877.00 1335.30 52.50 HISTORY ANDPRO FILE 35,66,498 13,55,688 3,45,399 12,30,260 3,07,907 1,11,000 1,65,394 50,850 Amount (Rs) Exp. 43.39 27.62 15.77 1225.32 8.47 180.06 151.69 9.76 875.34 1318.49 49.78 RE Network Project 0.00 0.00 0.00 77.00 0.00 0.00 0.00 0.00 0.00 77.00 77.00 0.00 (Rs.inLakhs) Exp. 0.00 0.00 0.00 76.60 0.00 0.00 0.00 0.00 0.00 76.60 76.60* 0.00 HISTORY ANDPRO FILE NBAGR jk-i-vk-la-C;wjks

9 ANNUAL REPORT 2017-18 FINANCIAL OUTLAY 10 ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks NBAGR Research Accomplishments Network Project on AnGR • Genomics&Evaluation ofAnGR • Genetic Characterization ofAnGR • Conservation ofAnGR • Identification &Characterization ofAnGR • Livestock Information Management • NBAGR

jk-i-vk-la-C;wjks

11 ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks

12 held on 4 Breed Registration Committee in its meeting Farm Animals New Registered of Breeds Indigenous Livestock Information rsngr and Krishnagiri Dharmapuri, manure in Salem, meat, and skin communities for Goundervellala Nadu are reared by Vanniyar and Kongu Black Salem goats Black Goat: Salem of Tamil yield rangesmilk from 270to 375kg. muddy fields forpaddycultivation. Lactation for carting and ploughing in the especially areBullocks excellent draft animals especially Uddercurved. and is small bowel shaped. medium insizeand is slightly backline the variable, mainly brown and grey. Hump is colourCoat is relatively short legs. and havehorned are sized, small Animals 79 Lakhs. about is population people. Totallocal draught by purposes distributed inentire state and reared for and milk cattle Cattle: of Lakhimi AssamLakhimi are duck, and geese donkey. camel, 7pig, 18chicken and one each of yak, cattle, 13buffalo, 28 goat, 42 sheep, 7 horse, 9 breeds is includes 169,which country inthe 41 duck and geese. Now total number of indigenous registered firsttime indigenous breeds of yak, geese, duckyak, and chicken. The institute has breeds of goat, and one breed eachof horse, pig, poultry. This includes one breed cattle,of two registration of ninebreeds new of livestock and Management Research Accomplishments NBAGR th August, 2017at New approved Delhi INDIA_CATTLE_0200_LAKHIMI_03041 INDIA_GOAT_1800_SALEMBLACK_06027

tolerance capacity inaridandsemi region. heat and drought excellent possess horse The great speed and stamina covering long distance. chal’‘Rewal its for Famous with performs it as docile temperament. Coat colour is mainly bay. bone length,broaderhoofforbettergripand pastern short back, short height, inch 60 to 56 other,each touching not but tips at curved ears features include roman nose appearance of face, 4 thousand.Unique population isabout Rajasthan. Total Barmer districts of Jaisalmer and Gujarat and Kachchh district of to native is breed Kachchhi-Sindhi Horse: with socio-culturalsignificance. used by local people for making traditional items are goats these from obtained fibres silky Long present. is Beard backwards. curved and sized small pointed, are Horns horizontal. are Ears patches on head, neck and legs. Head is straight. for its silky fibre. Coat colour is white with black 4,500. Itisamediumsizedgoatrearedmainly population isabout input. Estimated with almostzero open rangesystem tribes intraditional reared bySumi long hair goat” is known as“Nagaland Sumi-Ne Goat: and low mortality are main features of breed. this well maturity, placed. Early sexual multiple births backwards. In neck males, is thick, broad and that are upwards medium insize, curved and appearance. and males females Both have horns medium, semi-pendulous having leaf-like lean andtall, leggy. Colour is black. Ears are population is about 86thousand. These goats are ofErode districts Tamil Nadu. Estimated Sumi-Ne Goat of Nagaland also Sumi-Ne GoatofNagalandalso INDIA_HORSE_0417_KACHCHHISINDHI_07007 INDIA_GOAT_1400_SUMINE_06028 This indigenoushorse jk-i-vk-la-C;wjks

13 ANNUAL REPORT 2017-18 LIVESTOCK INFORMATION MANAGEMENT 14 ANNUAL REPORT 2017-18 LIVESTOCK INFORMATION MANAGEMENT Zovawk Plumage is dark is Plumage squat in posture. lakhs. population is 18.21 Assam in system production are ducks Pati Pati Duck: 1kg perday. massive and aggressive. Milk production is about weights rangesbetween206to416kg.Malesare body hair.Adult long with covered are legs and parts lateral ribs, belly, Yak.Brisket, Arunachali females. Horizontal ear is typical characteristic of than males in bigger are and colour in black curved, mostly are Horns stocky. and short are colour with medium sized and compact body. Legs yak is about 14 thousand. Predominantly black in of Arunachali population Estimated manure. and transportation fibre, for milk,meat, Monpa community These are reared by Arunachal Pradesh. Tawang districts of Kameng and West includes yak Arunachali of tract Yak:Arunachali Native in malesand59kgfemales. pig. Zovawk kg the Average54 is weight body of characteristics are mid-line on bristles long and line top belly,concave pot snout, concave ears, Erect boots. white and belly on patches forehead, white white spoton with black are purposes. Animals pork and manure for community reared byMizo 39 thousand.Itis Mizoram with anestimated population of about jk-i-vk-la-C;wjks rural areasof . Estimated These are Pig: Zovawk pig is distributed in distributed is pig Zovawk INDIA_YAK_2300_ ARUNACHALI_16001 INDIA_PIG_2700_ZOVAWK_09007 NBAGR INDIA_DUCK_0200_PATI_11001 reared in backyard in reared Research Accomplishments minimum inputsforrearing. hardy, diseaseresistantandforagersrequiring weighing about137g.Kashmir Anz geeseare each eggs/year white-shelled 12 about lays respectively. goose female, The and male in kg and white. Adult bodyweightis3.82and3.34 cinnamon of mixture a and white, cinnamon, is around the water bodies. Colour of the plumage located areas in hobby a as and feathers, and than 13,000birds. population ismore Kashmir. Estimated and Jammu of districts Budgam and Baramulla Ganderbal, Bandipora, geese is Srinagar, Native Kashmir Anz Geese: tract of Kashmir Anz body weightis1.58kg. average sacrifices. The ritual and egg meat, for used are ducks Pati yellow. predominantly are feet and shank bill, The sexes. both in neck at brown. A whiteringmayornotbepresent solid are Ducks feathers. white and black with tail head; black greyish with drakes in brown and 2.5kginfemales. males in kg 3.8 about is weight Averageg. body production is 67 per year with an egg weight of 46 egg Averagemales. in shoulder the over flow feathers hackle of plenty rudimentary; and small males. Beak is small, strong and instout. Wattlespresent are is Spur type. pea is Comb feathers. Males have golden yellow or red hackle and saddle black. predominantly is Plumage look. majestic have and slim, and 12,000. Birds are tall population isabout purposes. Estimated meat and (fighting) forgameOdisha Keonjhar districts of Mayurbhanj and in reared is chicken Hansli Hansli Chicken: They are reared for meat, eggs, They are reared formeat, eggs, INDIA_GEESE_0700_KASHMIRANZ_18001 INDIA_CHICKEN_1500_ HANSLI_12018 cattle. GaundluEdigi, communities are rearing this feeding. intensive including grazing and partly stall in Golkunda area. Management was semi different agricultural operations.Cows were bred areas, only were bullocks available and for used hock. Horns were and small stumpy. In these and concave or flat. Tail was short and abovethe black spots on hump and neck. Face was shorter colour insize. was grey with Body smaller light Ongole breed but are lighter in colour and Kamma cattle: maintained indifferent areas. for different agricultural operations and cows are that area in this only are bullocks reared and used farmers It of two districts. these was observed economic parameters were recorded from 22 Managementvillages. practices, utility and socio- and morphometric characteristics from 13 and were sex recorded for different physical Atotal ofdistricts. 90animals of different age conducted inMahbubnagar and Vikarabad of Vandharvi and Kamma cattle, was survey To characterize and establish breed characters Cattle Populations of Telengana State Identification & hrceiaino AnGR Characterization of Research Accomplishments NBAGR Telega, Madiga, Lambadi, Kapollu, Kamma bullock Kamma like look bullocks villages of werevillages 4districts recorded. Animals economic parameters (32farmers) from 10 management practices, utility and socio- different physical characteristics (84 animals), Jagithiyar Information (2 village) districts. on Asifabad Nirmal (4villages) and (1village) was conducted inAdilabad (3village), breedthe characters of Jhari cattle, Jhari cattle: To characterize and establish yield ranged from 1.0to 2.5kg. cows were bred indifferent areas.Daily milk werebullocks available for work agricultural and hock with black switch. areas, In only surveyed Hump and weredewlap moderate. Tail was upto were longer Ears were and horizontal. curved. area. Face was longer and concave Horns type. to other cattle populations available inthis colour.in body was larger Body as compared cattlethis breed. Vandharvi were bullocks white Munurkapnlu and Vajai communities are rearing in Warangal Telega, district. Golu, Lambadi, Vandharvi cattle: Vandharvi cattle are found Vardharvi cow Vardharvi Jhari cattle jk-i-vk-la-C;wjks survey survey

15 ANNUAL REPORT 2017-18 Identification & Characterization of AnGR 16 ANNUAL REPORT 2017-18 Identification & Characterization of AnGR funnel type. Temperamentfunnel type. was docile. shaped. Teats were 6-10cmlong and mostly Udder was not small, developed and mostly bowl touching ground. the were Legs long and strong. Tail was above hock with black switch (88%),not inlength andsmaller horizontal inorientation. (86%) and eyelids (91%)were black. Ears were proportionto body with other cattle. Muzzle majority and of cases the longer as compared cows. was Face small. Dewlap was concave in Hump was moderate and inbullocks in smaller as comparedsmaller to Ongole or its type. compared to Ongole was or its Body grades. i.e. longer inlength and at as thin base the other differentiating characters were horns for differentiating it from other cattle. The longer and convex is major the type, character shape. and Face,was small cylindrical is which Jhari cattle were (85%)in grey colour, body and considered can be for study. further one group uniform to be waswhich observed cattle. Among non-descript the cattle, there is Ongolethem and graded others non-descript mixed population of cattle is available, some of was natural. It that area inthis was observed feeding to only bullocks the at home. Breeding i.e. grazing only. Farmers provide supplement were reared on extensive system of management in the Koraputin the of district Odisha. Buffaloes Manda Manda buffalo: buffaloes are distributed Buffalo Populations Odisha of State jk-i-vk-la-C;wjks NBAGR Research Accomplishments Male and female of Manda buffalo registered as aseparate breed. and recognized population tothe be deserves are Manda Considering type. current scenario, is arounddistrict 20,000,out of 80-90% which Total estimatedpopulation buffalo in Koraput with buffaloes an average of 8 per family.animals farmers. The herdfrom size varies 5-20 adult as asource50,000 and of serves income to the pair of is bullocks at sold aprice of Rs.30,000to without8-10 km showing any signs of stress. A for transportation is 10quintal/day and up to laterite of soil area. this Their draughtcapacity animal for ploughing fields small of hard red of land in a day. It as an excellent serves draught pair has capability the of ploughing around 1acre ofinterval 1.5-2.5 years. On an average, a bullock firstcalving is invariably 4 yearswith calving a dependent fodder. on grazing and dry Age at extensive system of management and are purely average of Animals 2.5kg. are maintained on is low ranging day 2-4kgper with between an andmilk dung. yield inManda Milk buffaloes for draught and to some also purpose extent for are hardy and mainly maintained by farmers colour is lightBody blackish brown. Animals typicalheavy to seems which population. the with compactsmall body. Horns are long and that Mandawas observed buffaloes are uniformly Manda was buffaloes recorded.it During survey biometricbody traits of 69 adult maleand female uniform physical characters. Information on 18 variability from other Odisha buffaloes and have region inthis havesurveyed phenotypic observed for which wereobserved mechanization of is acausetract of concern, major reasons population of Sambalpuri buffalo in its native measurementsbody were recorded. Dwindling region confirmed it. Phenotypic characters and exchange of animals two states between inthe to recorded inChhattisgarhi buffaloes. Frequent bordering Chhattisgarh features had body similar in adjoining Bargarh and Jharsuguda districts and blocks of Redhakhol Sambalpur. Buffaloes mainly buffaloes in were observed Naktideul SambalpuriDeogarh districts, and Boudh type inSambalpur,tract Jharsuguda, Bargarh, visits During Sambalpuri buffalo: to breeding the Research Accomplishments NBAGR Discriminant analysis on body based measurements of buffalo Odisha populations Sambalpuri buffaloes of Odisha at 1%level of significance. significantlythe 4 between populations buffalo horn circumference, other traits all varied discriminant analysis. Except length and body groupingreveal distinct of populations by and Kalahandi of buffaloes Odisha could of Sambalpuri, Manda, Paralakhemundi 17 biometric traits recorded on 148 animals Analysis of data on measurements body of for draft purpose. of 2-4litres day. per Animals are hardy and good extensive system of rearing having potential milk production. Animals are maintained mostly on farm operations, and choice of Murrah for milk jk-i-vk-la-C;wjks

17 ANNUAL REPORT 2017-18 Identification & Characterization of AnGR 18 ANNUAL REPORT 2017-18 Identification & Characterization of AnGR and depth are visible on end distal of ear pinna Serrationswere inflocks. seen of different shape light brown, chocolaty and black colour patches patches around eyes, muzzle and on ear. The The coat colour whiteand face is tanwith colour Chitarangi animals are medium to large insize. knownalso as Shamaki and wali Ratani sheep. ofdistricts Rajasthan and nearby areas. It is Muktsar of district Punjab, Ganga Sri Nagar sheep population,type distributed inFazilka, Chitarangi wool sheep: Chitarangi is acarpet PopulationsSheep of Northern States production 1.5to The 2kg. cost of Feb- March of farmers reported average annual greasy wool June- July and October- November. The majority a year month inthe thrice of February- March, scarcity The sheep are period. generally shorn to pregnant the season, ewes,lambs and during concentrate during to sheep breeding especially on grazing, however, 55.36%farmers provide respectively. Sheep are primarily maintained 9.48 ±0.04,18.030.09and 22.19±0.17cm, ± 0.22,86.520.31,88.660.38,20.250.07, length and length tail were 72.54±0.24,73.70 paunch facelength, facewidth, ear girth, girth, The length,body height overall wither,at chest to and 95kg in males 26to 74kginfemales. respectively± 0.50kg, which varies from 39 andmales females were 56.27±1.28and 46.16 of breed. this The average adult body weight of animals, the is which in all characteristic the jk-i-vk-la-C;wjks Chitarangi male Chitarangi NBAGR Research Accomplishments to 2%.Theas high price these animalsof is very farmers reported that from twinning varied 1 in size, pendulous and drooping. Majority of by deposition the of fat tail. inthe Ears are large flocks during survey. The sheep are characterized black- white and brown –white in were seen also however some black animals/ and mixture of in size. The coat colourwhite is and brown; weightbody ofthat Dumba reflects it largeis money during Eidfestival. the and Biometry isDumba for or earning of purpose Qurbani ofdistricts UP. Main objective of rearing Malerkotla tehsil of Punjab and Muzzfarnagar animals of Dumbaare available also inDelhi, Nagour and Ajmer of districts Rajasthan. The Dumba is found sheep: Dumba mainly inJaipur, lambing. with anto October average of 80to 90%annual is January to March and minor is from August 71.43% of farmers. The mainlambing season at firstlambing is 17 to 18 months reported by reported by majority of sheep breeders and age first mating inrams is about 12-15 months as farmers. The ram-ewe ratio is 1:34.33 and age at lambs was reported as Rs. 3000 to 6000 by 55.36% 66.07% sheep farmers and cost the of surplus age of lambs male was reported as 6months by white incolour and others are canary. Marketing Nov clip (Rs.20-100).The Feb- March clip is 230; followed by June-July (Rs.30-140)and Oct- clip wool varies is which from higher Rs.80to Chitarangi female Chitarangi against rest the breeds. (1-vs-R) and the all by pairs taking of breeds (1-vs-1),asingle breed SangamneriMadgyal, and Solapuri) was made Lonand, Marwari, Munjal, Muzaffarnagri, Classification of eight sheep breeds (Kolhapuri, Traits Populations on Morphometric Based of IndigenousClassification Sheep of farmers. the percentage of about 95was reported by 63.64% The average ramsper flock is1.28. The lambing and Rijika. Breeding is through natural service. sheep. The major was loom, leaffodder of Ardu Farmers provide concentrate and fodder to these maintained on intensive system with no grazing. farmers kept sheep in covered area. The sheep are ewes and 3.48lambs. The majority of sheep DumbaThe flocks comprised of2.08 rams, 10.32 consisted of 15.88Dumbaand 0.16other sheep. size wasflock 16.04 (range: 2 flocksto 65). The and 17.69±0.22cm,respectively. The average ± 0.79,94.780.85,20.860.18,9.590.08 length were 71.88±0.55,76.070.60,93.38 facelength, facewidth, ear length andgirth, tail overall length, height, body paunch chest girth, and124 kg in males 35 to 96 kg in females. The respectively± 1.79kg, varies which from 50to of and males females were 83.89±3.95and 58.76 Rs. 25000-40000.The average adult body weight at rate the of Rs. 60000 to 125000 and lambs at claimed by sheep owners. Adult animals are sold Research Accomplishments NBAGR Dumba male Dumba of partition binary one-to-one (1-to-1) in female stored for of eachset data. According to type the providing classification best the results were (0.1,0.01,andset parameter 0.001).The values and 10).Parameter ‘decay’ was tuned inthe hidden layer -‘size’ was (3,5, tuned set inthe validation Number method. of inthe nodes Two parameters were tuned using 10-fold cross packageThe layer asingle hidden uses inANN. conducted using ‘nnet’ package inRsoftware. for of eachset data. Experiments on ANNwere tuned parameter values providing results best the were range inthe taken 2e1to 2e10for finding 10e-6 to 10e-2 and that of parameter Cost (C) of Gamma parameter (γ) were range inthe taken wereSVM model tuned on breed the data. Values ‘e1071’ package in R software. Parameters of Experiments on SVMwere conducted using experiments was calculated. thirty of data. Mean (±S.D.) of classification accuracy of conducted using tuned the parameters on each set ratio consisted of experiments 70:30. Thirty were for classification. the Training and testdata set and length, tail were included data inthe set withers, paunch chest ear length girth, girth, traits weight, length, body height viz. body at having profiled been seven morphometricon machine (SVM).Sheep 2-8teeth, aging between networksneural (ANN)and support vector discriminant analysis functional (DFA), artificial Methods of classification included classical Dumba female Dumba jk-i-vk-la-C;wjks

19 ANNUAL REPORT 2017-18 Identification & Characterization of AnGR 20 ANNUAL REPORT 2017-18 Identification & Characterization of AnGR of goats. . uniformity and purity within populations both of Bidar for district Bidri goats. There was and in Bidar, and Bhalkhi Humanabad taluks Chitradurga for district Nandidurga goats; Hosdurga and Molakal Muru of taluks Chitradurga, Challakere, Hiriyur, Holalkere, and Bidri goats. was conducted Survey in parameters on was collected Nandidurga economic, management and performance Information on morphological, socio PopulationsGoat of Karnataka State by ANNand DFA models. followed performed SVMmodel best the Overall, R(≥99%). inMarwari-vs- were observed and 0.896±0.008respectively. Highest accuracy were 0.857±0.005,0.886±0.006 accuracy model was inSolapuri-vs-R. The DFA, ANN and SVM rest (1-to-R), least the assignment (%) accuracy and ANNmodels. In partition binary the one-to- followed best performed SVMmodel the by DFA 0.812±0.031 inANNand SVMmodels. Overall, The corresponding values were 0.714±0.029 and (%) inSolapuri-vs-Madgayal (0.802±0.031). data, DFA the gave least assignment accuracy Muzaffarnagri-vs- R Munjal-vs- R Marwari-vs- R Sangamneri-vs- R Kolhapuri -vs-R -vs-R Madgyal Solapuri-vs- R Lonand-vs-R Type of partition jk-i-vk-la-C;wjks Correct classification rates classification Correct (%)using DFA, ANN and SVM(one to partition)rest NBAGR Research Accomplishments 0.959±0.009 0.931±0.008 0.991±0.003 0.899±0.002 0.897±0.009 0.897±0.009 0.864±0.004 0.936±0.001 DFA population. Total goat population of breeding There is uniformity and purity within Bidri Bidar and Kalaburagi of districts Karnataka. Bidri goat: Bidri goats are distributed in females. from 30 to 50 andkg in males 26 to 40 kg in meat. Twining is common. Adult weight varies Milking is not done goats as these are reared for brown or black. Ears are and pendulous. leafy on colour grazing.Coat is white and eyelids are kutcha sheds during night and are only raised areFlocks stationary. Goats are housed in 1,78,000. Average sizeis 29.6 (12-93). flock Estimated population of Nandidurga goat is population of breeding is 6.6lakhs. tract Davangere of districts Karnataka. Total goat distributed inChitradurga, Tumkur and Nandidurga goat: Nandidurga goats are 0.954±0.003 0.948±0.005 0.996±0.001 0.929±0.009 0.901±0.007 0.914±0.008 0.896±0.009 0.948±0.005 ANN Nandidurga goat 0.967±0.006 0.959±0.007 0.994±0.003 0.924±0.006 0.935±0.007 0.923±0.009 0.897±0.008 0.946±0.004 SVM less innumber. Yaks inNubra and other regions census, 2012),however, pure may yak account & Kashmir are about 54thousand (Livestock Yak and of yak-cattle Jammu crosses inLadakh system. populations and documenting production their yak to characterize local ofSham Ladakh valleys conducted indifferent villages of Nubra and and UVradiation. high were Breed surveys altitude conditions -hypoxia, low temperature distinctive flora and fauna adapted the high to cold-arid region is well known for possessing (Himachal Pradesh) of Indian Territory. This andmeter Lahaul-Spiti (J&K) MSL) of Ladakh conditions –occupies altitude high areas (>3000 inhabited region with extreme climatic North Trans-Himalaya world’s –the highest Yak Population (J&K) of Ladakh commonly observed. and 24to 40 kginfemales and twinning is Adult weight varies from 31 to 47 kg in males pendulous. Goats are reared for meat only. muzzle, eyelids and hooves are black. Ears are open sheds during night. colour Coat is black, areFlocks stationary. Housing is inkutcha is 1,10,000. Average size is 74.3(21-130). flock and is estimated 5.2lakh tract Bidri population Research Accomplishments Adult weight Tail length Ear length Ear Horn size Height at withers Body length Body Chest-girth Chest-girth Parameter NBAGR Body measurementsBody (cm)and adultweight (kg)of Nandidurga and Bidri goats 38.92±2.95 21.29±0.91 16.36±0.41 20.33±1.54 79.40±1.83 59.65±1.56 81.47±2.40 Avg. ±SE Male (46) 26-56 13-26 13-19 11-30 72-95 48-69 70-98 Range Nandidurga 30.11±1.03 18.90±0.43 15.38±0.29 15.79±0.73 71.92±0.72 55.31±0.87 75.07±1.02 Avg. ±SE Female (172) hair Different and high. set morphometrictraits covered with long hair. Tail with long is small region. Flank, lower and regions belly thigh are and horizontal. Pelvis is than wider shoulder backward with pointing tip. Ears are medium Horns and are laterally placed curved upward and concave on back. Limbs are and small cylindrical. Dorsalmales. line is convex on wither (ridge) and hump is Dorsal ridge small. is prominent in prominent. Forehead and bone nasal are straight, ofpart coat the and switch. Occipital bone is white possess patches also yaks on to small large switchtail are black, horns are grey to black. Few black with sheen. glossy Skin, muzzle, eyelids, temperament. colour Coat is dark brown to of Nubra are medium insizeand moderate in manure, hair-fibre transport and purposes. Yaks located at altitudes higher for meat, milk, areof reared Ladakh by Buddhist community 24-41 17-29 12-20 7-23 62-82 44-63 61-90 Range 36.78±2.76 17.17±0.65 16.25±0.37 16.44±1.85 79.25±1.79 58.17±1.46 80.75±2.12 Avg. ±SE Male (20) Bidri goat 23-52 14-21 15-18 9-26 72-89 52-71 68-89 Range Bidri 32.36±0.90 16.70±0.46 16.28±0.22 13.72±0.67 74.84±0.66 56.09±0.63 77.12±0.69 Avg. ±SE Female (43) jk-i-vk-la-C;wjks 19-45 11-23 12-19 6-20 64-83 47-70 64-87 Range

21 ANNUAL REPORT 2017-18 Identification & Characterization of AnGR 22 ANNUAL REPORT 2017-18 Identification & Characterization of AnGR used solelyused for feeding of Herd calf. sizeis 6to mostly once inmorning. upto Milk 2months is to 8months only. Female is (Demo) milked yak to 1.0Kg/day. ranges period from Lactation 6 during April-May. production Milk of is 0.5 yak major breeding whereas calving occurs period, 8 years. July-August months during summer is out generally. Males are castrated at age the of breeding is preferred; is carried no selection year and produces 7-8calves inlife time. Open at about 4years of age alternate and calves every maturity at 3years of age. calving starts Demo and bowlshaped. Male and female attain sexual given table inthe below. Udder small is very were measured for and male females are which Tail length with switch Tail length (without switch) Paunch girth Chest girth Body length Body Height at wither Height upto elbow Ear length Ear Face length Face width Distance horns between Horn circumference Horn length Morphometric Trait jk-i-vk-la-C;wjks Male yak of Ladakh of yak Male NBAGR Morphometric traits (incm)of Yaks of Ladakh 67.53±7.98 38.53±5.57 161.82±17.07 160.85±17.23 116.61±13.83 111.24±10.27 58.9±4.47 12.66±1.20 45.93±4.43 22.41±2.61 20.40±3.53 23.37±2.83 45.42±6.70 Mean ±SD Adult Male (n=40) Research Accomplishments 11.8 14.5 10.6 10.7 11.9 9.2 7.5 9.4 9.7 11.7 17.3 12.1 14.8 C.V. transport purposes. transport purposes. 2-4 Kgmilk/day. for Dzo is used ploughing and Girimo (F3)) insomeDzomo cases. produces other generations (Garu/Garamo (F2),Giri/ mostly for F1generation (Dzo/Dzomo), and hybrids (using as are amale) yak maintained make adaptable them to lower altitude. Cross- visitedthe area, to and increase particularly milk are largely being preferred over inmost yak the of are prevalent.diseases also Cross-hybrids of yak followed inNovember and April months. Skin outbreaks are common; vaccination for FMDis and remain there upto October/November. FMD and migrate towards land high pastures inApril 30. Most of are yaks the reared under pastoralism

62.45±7.82 36.25±4.76 146.20±11.96 147.2±11.37 106.86±9.04 100.86±7.27 53.73±4.02 12.48±0.89 41.64±3.04 18.46±1.50 16.00±1.79 15.58±1.89 31.93±5.13 Mean ±SD Adult Female (n=65) Female yak of Ladakh of yak Female 12.5 13.1 8.2 7.7 8.5 7.2 7.5 7.2 7.3 8.1 11.2 12.1 16.1 C.V. 1.08 1.06 1.10 1.09 1.09 1.10 1.09 1.01 1.10 1.21 1.28 1.50 1.42 index Dimorphism

Sonam, Namgyal, Tundup, mostly Dorje; inrural by Buddhist communities – Tsering, Tashi, about fivethousand. donkey is Ladakhi reared Total donkey population of is indistrict Leh PopulationDonkey (J&K) of Ladakh Research Accomplishments Estimated weight body (kg) Hoof circumference (HL) Hoof length (HL) Pastern circumference (HL) Pastern length (HL) Canon circumference (HL) Canon length (HL) Gaskin circumference Gaskin length Hoof circumference (FL) Hoof length (FL) Pastern circumference (FL) Pastern length (FL) Canon circumference (FL) Canon length (FL) Fore arm circumference Fore arm length Tail length-with switch Chest width Neck length Ear length Ear Face width Face length Paunch girth Heart girth Body length Body Height at wither Morphometric Trait NBAGR Morphometric traits (in cm) of adult donkeys of Ladakh Morphometric traits of (incm)of Ladakh adultdonkeys 82.88±9.28 20.85±1.68 5.1±0.57 12.54±1.05 6.87±0.64 13.12±1.23 25.8±1.47 25.23±2.09 42.4±3.14 22.57±1.4 5.43±0.46 12.25±0.61 7.37±0.48 12.43±0.83 17.2±1.37 20.5±2.02 39.13±2.39 50.53±6.93 21.63±2.21 27.27±3.58 21.33±1.72 20.07±1.16 46.07±2.6 111.07±8.69 101.6±5.14 95.53±5.66 94.27±3.75 Adult Male (n=15) Mean±SD 64-100 17-23 4-6 11-15 6-8 11-15 24-29 23-30 35-47 21-25 5-6 11-13 7-8 11-14 15-19 18-26 33-42 41-63 19-26 22-32 19-25 18-22 42-51 100-128 93-115 82-103 87-101 Range sized andsized docile. and, secondarily for manure. Animals are medium people and supply food-logistics by Indian Army construction/trekking-camping materials by local transportation of manure/fuel-wood/fodder/ areas. It primarily is used as apack animal for 8.06 11.18 8.37 9.32 9.38 5.70 8.28 7.41 6.20 8.47 4.98 6.51 6.68 7.97 9.85 6.11 13.71 10.22 13.13 8.06 5.78 5.64 7.82 5.05 5.92 3.98 CV% 20.33±1.42 78.97±18.03 5.1±0.52 12.14±0.69 6.62±0.77 12.69±0.89 25.89±1.34 24.38±2.41 41.26±2.93 21.9±1.3 5.25±0.56 11.65±0.68 7.17±0.64 11.94±0.55 17.37±1.65 19.85±2.0 38.4±2.52 51.47±5.91 19.92±1.74 27.51±4.21 22.39±1.99 19.19±1.48 47.28±2.88 109.55±6.5 102.96±5.58 97.46±7.37 93.85±3.8 Mean±SD Adult Female (n=54)

17-23 44-127 4-6 10-13 5-9 11-16 23.29 20.32 32-46 20-25 4-7 10-13 5-8 10.5-13 14-21 16-30 34-44 36-67 14-25 17-36 17-26 15-25 42-58 97-121 92-118 81-116 86-106 Range Range 6.98 10.20 5.68 11.63 7.01 5.18 9.89 7.10 5.94 10.67 5.84 8.93 4.61 9.50 10.08 6.56 11.48 8.73 15.30 8.89 7.71 6.09 5.93 5.41 7.56 4.05 CV% jk-i-vk-la-C;wjks 1.03 1.05 1.00 1.03 1.04 1.03 1.00 1.03 1.03 1.03 1.03 1.05 1.03 1.04 0.99 1.03 1.02 0.98 1.09 0.99 0.95 1.05 0.97 1.01 0.99 0.98 1.01 Index Dimorph.

23 ANNUAL REPORT 2017-18 Identification & Characterization of AnGR 24 ANNUAL REPORT 2017-18 Identification & Characterization of AnGR hrs with 40-50 Kg load in high terrain.hrs inhigh with Animals 40-50Kg load easily (Bonchangs) and can travel 15-20Km in5-6 donkey has abilityLadakhi to cross ridges small As in few cases. are uniqueness, observed the vaccination is done. Tympany, skin or eye diseases along fodder is provided. with dried jiggery No a mixture of wheat, salt, mustard oil (chhak), provided. pregnancy During and calving, Barley stored and fodder (Alfa-alfa) crop residues are on grazing in summer. winter During and snow, during night loose them also. Animals are raised separately during night. Some households keep or pucca) either along with other livestock or housed inenclosures or animal houses (kutchha household.1-6 donkeys per Animals are mostly for isMilk used feeding of foals only. Herd sizeis breeding is preferred, out. is carried no selection year and produce 5-7foals inher lifetime. Open is March-June. donkey foals alternate Ladakhi 4.5-5.5 years. Major of season breeding and foaling of first breeding is 3.5-4 years and firstis foaling donkey areLadakhi given intable. In females, age convex. Various morphometric characteristics of is straight to slightly concave. Forehead is flat to and dense on lateral sidesof body. the Nasal bone irrespective of Hair sex. coat is medium and curly, around in most eyes are of animals the observed mark around muzzle and mouth and white ring black with light coloured or white belly. White colourCoat varies from light to dark brown and jk-i-vk-la-C;wjks Male Ladakhi donkey Ladakhi Male NBAGR Research Accomplishments AnGR and inHaryana Punjab States Programmes for theManagement of Evaluation of Policies Livestock and manure and considered for good to be soil fertility. Donkey manure is mixed with human and cattle are sure footed and innight track easily also. were recorded. also of animal husbandry and problems by faced them pig were and consulted poultry for activities their kinds ofAll farmers keeping cattle, buffalo, goat, informationcollect as Q-1and four VOs Q-2. as per atotal21 villages, of 42farmers were to interviewed made to Tarnalso Taran of district Punjab and from usingcollected questionnaires. the One visit was farmers attending Kisan Mela at GADVASU was Punjab states were Information collected. from 16 andpolicies programmes of AHD Haryana and and Ludhiana) were for selected study. the Existing and Palwal) and Punjab (Tarn Taran, Ferozpur each in Three districts (Karnal,Haryana Hisar farmers/livestock keepers and veterinarians. were for developed information collecting from GADVASU, Ludhiana. Two of sets questionnaires was initiated in July 2017incollaboration with ResourcesGenetic inHaryana and Punjab states” programmes for management the of Animal projectnew “Evaluation of livestock and policies With extension of mandate the of Bureau, the a Female Ladakhi donkey Ladakhi Female of animal. marginal Ear tissues from 8 and without any limitations of or sex age ofbecause accessibility, easy non-invasiveness In addition, cells skin offer an attraction to advancement of culturecell techniques. precious livestock genetic resources due proposebanks approach apractical to preserve Manipuri horse and Kutchi Fibroblast camel: Banking Somatic Cell following table. procured during year this is given inthe sperms and somatic cells). The germplasm germplasm embryos, DNA, (semen, epididymal strengthened by diversified preserving form of The germplasm repository at NBAGRbeing is situEx Conservation AnGR of Conservation Research Accomplishments Germplasm cryopreserved during 2017-18 Germplasm cryopreserved Total Cells Somatic Semen Germplasm NBAGR Horse Camel Cattle Species Marwari Kutchi Nagori Khillar Rathi Dangi Bargur Sahiwal Gir Kankrej Breed Explant culture of fibroblast cells 24775 400 400 4000 7000 2475 6000 3000 500 500 500 vials doses/ No. of vials (1×10 vials stocking at least 75 cryogenically-preserved were cryopreserved fromwere 3 cryopreserved horse and Kutchi camel respectively. The cells 0.76 population doubling/24 hrs for Manipuri rate of 0.86population doubling/24 hrs and of 27.9hrs and 31.37hrs with multiplication plateau phase with population doubling time through alag phase, alogarithmic phase and a typically S-shaped as cell the population passed atThe growth passage-5 curve represented dependent inhibition during proliferation. cell likewhirlpool migrating patterns and density exhibitedFBS. Cells radiating, like or flame DMEM+Ham’s with 10% F12(1:1)media horse and Kutchi camel was continued using cells. Passaging of lines cell for Manipuri both cells grewrapidly and replaced epithelial the but during subsequent passages, fibroblast epithelial and fibroblast cells together grew with centrally located oval nuclei. Initially like cells showed typical fusiform morphology Manipuri horse and Kutchi camel. Fibroblast- sprouting from margins the of explants of both like and fibroblast-like cells seen could be (HiFibroXL™). At about 10-14days, epithelial- technique with fibroblast media specific culture was established using explant culture cryopreservationcell technique. Primary attachment culture cell followed method by forutilized establishment of lines cell by Manipuri horses and 6Kutchi camels were horse and Kutchi camel genetic the preserves established These newly lines cell of Manipuri 6 cells/ml) per animal. per cells/ml) rd to 6 to jk-i-vk-la-C;wjks th passage

25 ANNUAL REPORT 2017-18 Conservation of AnGR 26 ANNUAL REPORT 2017-18 Conservation of AnGR respective Gaushala. Among identified the ear and tagged kept in separate enclosures at and soundness. health Identified cows were and Uplana) on physical based characteristics Gaushalas (Shri Krishan Gaushala Jundla, Nising were grades their selected identified the 3 in Cows of Hariana and breeds Sahiwal and Cattle at Gaushalas and Hariana of Sahiwal Conservation and somatic cloning cell research. invaluable materials for genomic, post-genomic resources at cellular the level and provides jk-i-vk-la-C;wjks Growth curve of fibroblastGrowth curve cells NBAGR Research Accomplishments Hariana calves in Gaushala born it from varied 12to 20kg. calves ranged from 14 to 18 kg and in Hariana, ranged from weight 1.0 to Birth 4.0 kg. of Sahiwal In9.0 kg. Hariana, fortnightly yield daily milk fortnightly yield ranged daily milk from 4.0to (24 F+17M))calves were In born. Sahiwal, total of (4F+1M)and 46(5Sahiwal 41Hariana pregnancies were confirmed in Hariana cows. A (CIDR) treatment was applied to 10cows and 4 was given.Controlled The InternalRelease Drug reproductive status and accordingly, treatment were examined by ultrasonography for their Department, Karnal. In Jundla Gaushala, cows with help the of State Animal Husbandry camps were organized three the Gaushalas inall mixturemineral supplementation. Two health cows were given deworming treatment and containers Gaushalas. to these selected the All refilling liquid nitrogenmonthevery inLN2 containers three the Gaushalas. to all They are Department, Karnal has provided liquid nitrogen Sahiwal, respectively. State Animal Husbandry inseminated with elite of semen male Hariana and cows, 52cows of Hariana and 4of were Sahiwal was reflected inF was to closer other Same Odisha buffaloes. When compared, Chhattisgarhi buffalo, however, populationsbuffalo Odisha. of differentiated highly genetically from other all markers data analysis based indicated Manda being structure and evolutionary lineages. Microsatellite population to delineate was used genetic the D-loop sequence of 24animals of breed/each Sambalpuri and Manda as well as mitochondrial populations- Kalahandi, Chilika, Paralakhemundi, markers on 48animals eachof five Odisha buffalo dataGenotype generated for 20microsatellite Populations DiversityGenetic of BuffaloOdisha Genetic Characterizationof AnGR KH-Kalahandi, CG-Chhattisgarhi, SBP-Sambalpuri, MD-Manda) Research Accomplishments (MU-Murrah, NR-Nili Ravi, CH-Chilika, PM-Paralakhemundi, 0.167 0.147 0.210 0.149 0.146 0.143 0.035 0.000 Murrah Phylogenetic analysis using microsatellite allelic NBAGR diversity Nei’s based distance genetic Breed-wise Fst based genetic differentiation Fst based Breed-wise with Odishaof other buffaloes 0.183 0.161 0.219 0.166 0.152 0.157 0.000 Nili Ravi st based principal component based 0.048 0.040 0.093 0.028 0.031 0.000 Chilika 0.045 0.042 0.079 0.023 0.000 P’mundi 0.037 0.037 0.087 0.000 K’handi partitioned populations the into 7groups, atbuffalo K=7,having maximum support, breeds and including Murrah and Chhattisgarhi structure analysis out carried on five Odisha Microsatellite frequencies allelic based buffalonew breed. adistinct populationbeing fit for registration as Manda grouped separately, again indicating this into distant cluster. Within Odisha buffaloes, whereas Murrah and Nili grouped Ravi together of Odisha and Chhattisgarhi buffalo together, phylogenetic analysis showed also clustering analysis also. Nei’s genetic distance based Clusters inferredClusters STRUCTURE from 2.3.4(admixture model) including fiveOdisha buffalo populations and 0.085 0.083 0.000 Manda Murrah alongwithChhattisgarhi buffaloes 0.045 0.000 S’puri 0.000 Ch’garhi Ch’garhi S’puri K’handi Chilika Manda P’mundi Nili Ravi Murrah jk-i-vk-la-C;wjks

27 ANNUAL REPORT 2017-18 Genetic Characterization of AnGR 28 ANNUAL REPORT 2017-18 Genetic Characterization of AnGR (FIS) were estimated for Chitarangi and Dumba andloci within population inbreeding estimates information content (PIC) of different microsatellite heterozygosity (He) along with polymorphism of alleles (Ne), observed (H of (Ne), alleles observed variations and effective number observed viz. Different measurements withinof breed genetic Sheep Dumba DiversityGenetic of and Chitarangi groupedbuffaloes into adifferent sub-cluster. clusters but Odisha within riverine buffaloes, riverine and swamp into buffaloes distinct also. Phylogenetic analysis grouped also had more number of haplotypes breed specific and swamp Chilika between Chilika buffaloes. interesting was sharing part of haplotypes haplotypes were grouped separately. Most Manipuri swamp with unique buffaloes haplotypes shared being by riverine buffaloes. and swamp indicated buffaloes three major network with other reported Indian riverine comparative analysis using median joining D-loop sequencing of and Odisha buffaloes Evolutionary studies using mitochondrial Kalahandi buffalo. Paralakhemundi had maximum admixture of Manda and Murrah most being distinct. jk-i-vk-la-C;wjks Median joining analysis network on mitochondrial based haplotype NBAGR o sharing among different buffalo populations buffalo amongsharing different ) and expected Research Accomplishments F genetic variability populations. inthese The mean implied presence the of substantial amount of gene diversity heterozygosity) (mean expected diversity (mean and number alleles) of observed in Dumba, respectively. The estimates allele of 0.636 and 0.712 in Chitrangi and 0.689 and 0.749 values heterozygosity and expected values were latter.in the The heterozygosityaverage observed to 8.860(OarCP0049) with amean value of 4.852 value of former4.171 in the and 1.246 (CSSM47) 1.612 (CSSM47) to 8.828(CSSM31) with amean of populations inboth alleles and ranged from of number was lower alleles than observed the a mean of 10.125inDumba. Effective number and 5(BM757)to 19(BM1314,CSSM31) with 16 (CSSM31) with amean of 9.875inChitrangi number of ranged alleles from 4(OarAE129) to sheep. inDumba alleles distinct The observed across 24markers the inChitarangi sheep and 243 0.71. Atotal of were 237 distinctalleles identified informativehighly with average PIC value of population. markers the All were found to be polymorphic to be investigated inthe observed sheep. The microsatellite amplified loci were observed positive F observed the in deficiency number of heterozygotes. The 0.096 and two0.043 in the populations indicating populations might due be to non-random the IS (within population inbreeding estimates) was IS in the investigated inthe sheep and Principal Coordinate Analyses. assumedcan be on basis of the assignment tests absent. Distinctness of two the goat populations detectably large, recent genetic bottleneck was also in Bidri and 2.5%inNandidurga goats. Any populations not was also significant being 3.9% two populations. Heterozygote deficit these in theconfirmed sufficient level of diversitythe in 0.58±0.06 and 0.60±0.05 for Bidri and Nandidurga of Estimates alleles. heterozygosity, of observed population had 8.22 with mean of 3.58±0.44.Similarly, Nandidurga from 1.043(OarJMP29) to 8.804(OMHC1) number (16) of of Expected varied alleles. alleles with mean was number 8.48±0.88 alleles of observed polymorphism in Bidri goats was evident as the assortment was assumed. loci of the all Reasonable no significant P test for genotypic linkage disequilibrium yielded Nandidurga and Bidri goats, respectively. An exact and were 195alleles in across detected loci these markers were polymorphic and atotal of 189 a battery of 23 microsatellite markers. the All on genotype the based data generated by using Population genetic parameters were calculated and Bidri Goats DiversityGenetic Status of Nandidurga appears most the to probable be explanation. in differentflocks villages the of distribution area (Wahlund effect) due samplingto from different The existencepurpose. populationof substructure mating and of use fewer rams for breeding the Research Accomplishments 2D plot of the Principal Coordinate Analysis depicting ILSTS033 showing highest number the relative goat positions of two populations NBAGR values and therefore, independent ±0.66

mean observed number mean observed at NDRI, Karnal were to selected Karan Fries and 20of Sahiwal cells ofcells Karan Fries: somaticExpression analysis in milk of β-casein cattle (0.04). (0.19) followed by exotic (0.13)and Indian native offrequency was highest incrossbred Ballele cattle across arg) three the cattle was The observed types. variants, other than A1/A2only Bvariant 122 (ser and crossbred cattle. Amongst 13known the more than 1500samples of Indian native, exotic known variants of was estimated β casein across and(0.1). Allelic genotypic profile forthirteen indigenous cattle (0.01)and highest inexotic cattle least common genotype A1A1 was minimum in cattle was much lowerfrequency (0.09). The of proportion of A1A2genotype inIndian native of 0.41and arange of 0.40-0.43.The comparative well samples as semen with an average frequency proportionhigher in exotic and crossbred cattle as other0.59. Onthe hand, A1A2genotype was in (Indigenous cattle) with an average of frequency and ranged from 0.49 (exotic cattle) to 0.90 was predominant analyzed the cattle inall types of frequency (0.05).The A2A2 A1allele genotype (0.29). Indian native cattle minimum the revealed by samples semen (0.30)and crossbred cattle was highest inexoticallele cattle (0.31),followed The thatanalysis frequency the revealed of A1 livestock development boards of different states. samples were from collected stations/ semen and samples. semen The breeding semen bulls' Indian native (30breeds), crossbred, exotic cattle was estimated inmore than 4000animals including frequency allelic The allele of A1/A2 casein of β of β- A1/A2 alleles Health Implication of A1/A2Milk Variantsβ-casein inIndian and Cows Genomics &Evaluation of AnGR casein gene inIndiancasein cattle:

A total of 56 animals, 36 of cows maintained jk-i-vk-la-C;wjks check the check

29 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR 30 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR difference wasobserved. significant no hence similar, almost was peak and early mRNAat of value mean The stages. subsequently decreased in mid and late lactation by early with mean 8.9and then further peak with mean value 9.8 was highest followed at abundance mRNA The stages. late and mid than stage lactation peak and early in higher P<0.05) (P=0.0014, significantly was β-casein of theexpression andKaranFries, Sahiwal both in that revealed values) CT on (based data expression mRNA β-casein of Analysis A1A1/A1A2 genotype. low and for Sahiwal, none of animal the showed as number of KFcows with A1A1genotype is across lactation the stages for sample collection, cows, only one genotype traced (A2A2)could be genotypes (A1A2and A2A2)and for Sahiwal to isolate somatic cells. For Karan Fries cows, two mid (100-140days) and late (>215days) lactation lactation early (20-50 days), (5-15- days), peak samples were during collected different stages of lactation the stagesall before involution. Milk animalsthat were selected the able to complete yield records of current parity. Thedata showed of data of previous their parities and milk their stages. These animals werebasistheselected on expression profile of jk-i-vk-la-C;wjks

Allelic andgenotypicfrequenciesof A1/A2 variantβcaseininIndiannative,exotic, β-CN across lactation the NBAGR

Research Accomplishments crossbred cattleandsemensamples stages of However lactation was observed. the across A2A2and A1A2genotypes at different (P<0.05) differencethe expression in of β-casein could not included be study. inthe No significant stage, animal the belongingdry to A1A1genotype A1A2 genotype throughout in lactation. the Being expression of inA2A2genotype β-casein and on genotype their to basis delineate differential the Further KaranFriesanimals the were grouped also lactation stages. aand brepresent the statistical difference Relative in mRNA gene Karan of β-casein level Fries and Sahiwal milk somatic during cells different

(P=0.0014)

GAPDH> RPS23> UBI> HPRT1> RPS9> stable genes were, ACTB> EEF1A1>RPL4> stably expressed genes and most stable to least analysis identified EEF1A1 and ACTB as most analysis to ICGs. identify used The was also and RPS15was least stable. Further, Normfinder were ranked as most stable with lowest Mvalues HMBS> UBI> RPS15.EEF1A1and RPL4 genes ACTB>GAPDH> RPS23>RPS9>HPRT1> in decreasing stability order as EEF1A1=RPL4> exclusion resulted method in ranking of ICGs expressionhigher stability of agene. Stepwise cells of KF expression stability somatic derived milk inmilk candidate reference genes according to their were chosen. geNorm analysis to was rank used HMBS, UBI, GAPDH, RPL4, RPS23,and ACTB) candidate genes (EEF1A1, RPS15, RPS9, HPRT1, different lactation stages of Karan Fries, 10 evaluation of potential internal control across Identification of internalcontrol genes: For incomparisonhigh to A2A2. mRNA abundance of incase A1A2animals was Research Accomplishments Relative in mRNA gene KFmilk of somatic β-casein Statistical in level cells A2A2and A1A2genotypes. difference in

Ranking ofreferencegenesbased ongeNormand NBAGR . The lower Mvalue is indicative of Normfinder analysis expression was determined by the unpaired t-test. (P<0.05) genotype ifany. reveal association of phenomic data with specific differentcattle populations being carried is out to cows of A1A,A2A2and A1A2genotypes across Comparative profiling phenomicof milk data in progressiondisease are inmicemodel underway. relationship of A1A1, A2A2 and with A1A2 milk prepared and studies related cause and effect different casein genotype variantsbeta been has analysis. Also,further powder whole milk of and different tissues have for collected been appeared. Later, animals the were euthanized symptoms the 400ug), till for hyperglycemia ranging from 50ugto 400ug(50,100,200and animals group) ineach indifferent concentrations injection to four differentgroups of animals (8-10 was administeredBCM7/9/7+9 by intra peritoneal progression withpeptide disease inmicemodel. for cause the and effect relationshipBCM7 of ICAR-NDRI, to establish evidence experimental Workmodel: on was initiated animal trials at Association diabetes in mouse of with BCM7/9 value of 0.193. combination best to be of genes with stability RPS15>HMBS. EEF1A1and ACTB were found expression. are regarded as key players influencing gene histone modifications, chromatin remodelling) Epigenetic modifications (DNA methylation, in Bulls Promoter With Varying Motility Semen Methylation Status of DAZL Gene DAZL

gene plays an important role jk-i-vk-la-C;wjks

31 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR 32 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR to the poor motilityto poor the Frieswal bulls. This suggests values the were cases, both lower as compared former (15.21%)than latter the (4.34%)but in Friesian, epigenetic marks were more inthe In purebred counterparts, and Sahiwal Holstein (15.21%) and motility high phenotype (6.52%). motility (28.26%)as compared to medium of significantlyrevealed higher DNA methylation cloning vector. in pTZ57R/T analysis Sequence The amplified products weresequenced after 1 of DAZL gene using bisulfite converted DNA. were to amplify used of part promoter and exon DNA was isolated. Methylation specific primers motilitysemen parameters were and collected bulls XHolstein (Sahiwal Friesian) with varying from Sahiwal, Holstein Friesian and Frieswal crossbred cattle subfertility. samples Semen analyzed inan attempt to evaluate its role in DAZL and parents. their The methylation profile of correlated testes inthe of cattle-yaks hybrids of gene this have significantly been negatively The methylation and mRNA expression level in Position of methylated CpGsites in differentgroups (PQ:Poor motility, MQ: Medium motility, GQ:Good motility, HF: germline development and gametogenesis. jk-i-vk-la-C;wjks DAZL gene promoter inbull spermatozoa was gene inFrieswal bulls with poor NBAGR Research Accomplishments Holstein Friesian Holstein measures for LDis markers, one of most the commonly used parameters inbovines. islands could possibly influence reproductive that differential hypermethylation the of CpG Linkage disequilibrium analysis: disequilibrium Linkage Buffaloes Genome Wide Diversity of Indian Murrah, Nagpuri, Nili Ravi, Pandharpuri, UP, Jaffarabadi, Kalasthi, Kerala, Mehsana, Surti, (Assam, Manipuri, Banni, Bhadawari, Chilika, belonging to 17different breeds/populations linkage disequilibrium. Atotal of 625animals (GBS) was to utilized estimate values the of generated using Genotyping by Sequencing swamp are buffaloes) not known. The data for Indian the (riverine as buffaloes well as The linkagelocus. disequilibrium values atabsence allele of second the a particular and other the representing presence the or at allele absence of first locus the aparticular variables –one representing presence the or correlation two indicator between coefficient ) r 2 , the square of the , the

For biallelic pairs analysed and average r The various distance bins, number of SNP using PLINK software v1.9. estimation of linkage disequilibrium was done and were common individuals. to all The By GBS,atotal of 23,306SNPs were genotyped Tarai and Toda) were for utilized purpose. the given following inthe tables. distance for riverine and swamp buffaloes is Research Accomplishments

2 50-100 kb 25-50 kb 20-25 kb 10-20 kb 5-10 kb 0-5 kb Distance 50-100 kb 25-50 kb 20-25 kb 10-20 kb 5-10 kb 0-5 kb Distance Statistical information for LDover various Measure of LD (r ) Statistical information for LDover various NBAGR distances inriverine buffaloes distances inswamp buffaloes LDdecay in riverine buffaloes 37415 26541 20546 19113 16203 14553 pairs Number of SNP SNP pair distance 57250 37177 25607 23725 19791 17539 SNP pairs Number of 2 obtained for each 0.13 0.18 0.23 0.25 0.29 0.32 Average r 0.17 0.21 0.25 0.27 0.31 0.33 Average r 2 2 utilised forutilised creation the of areference family of The chip consists of 675,000 SNPs be and shall Density chip Buffalo for Murrah buffaloes. have completed SNP identificationwith High Genomethe Wide Association Studies, we Taking into account requirement the for SNPs required be shall for swamp buffaloes. riverine buffaloes and approximately 1,20,000 approximately 1,50,000 SNPs required to be for for swamp the This translates buffaloes. to and riverinethe buffaloes value of 25-50 kb get genetic the distance block of 20-25 kb for generally assumed that r the value of r 0.2. Thoughthere is criterionno fixed the for beyond 20-25 kb yield r populations (Riverine). The values of distance (Swamp)buffaloes and other 15buffalo all out carried was for also Manipuri and Assam Breed linkage wise disequilibrium analysis distance with r mapping experiment. If we only take genetic the numberthe of markers for needed an association genome with distance. It to determine is useful graphsThe howreveal fast LDdecays acrossthe distance for riverine both and swamp buffaloes. Thus,the values for LD arebetween 20-25Kb utilisation for genome association wide studies. than 0.2for its usage inlinkage disequilibrium Measure of LD (r2) 2 to be statisticallyto be significant but it is LD decay in swamp buffaloes SNP pair distance 2 value of greater than 0.2,we 2 values were less than 2 should greater be jk-i-vk-la-C;wjks

33 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR 34 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR and Manipuri. The ofbuffaloes Manipur and exceptcountry Mehsana, Chilika, Toda, Assam for most of populations buffalo the of the The analysis degree high reveals of admixture coefficients. populationsthe were estimated using ancestry locations. The admixture proportions foreach of populations belonging to different geographical software with K =17 theFaststruct i.e number of breeds/populations. Thedata was usinganalysed for identification of admixture among buffalo total of 23,306 genome SNPs wide were analysed SNP based diversitySNP analysis inbuffaloes: based implemented by DADF. of Enhancement of Productivity Bovine being Centre (indigenous breeds) under Mission the the under buffaloes NationalBovineGenome Total Toda Tarai Surti U PBuffaloes Pandhpuri Niliravi Nagpuri Murrah Mehsana Manipuri Kerala Kalasthi Jaffarabadi Chilika Bhadawari Banni (Swamp) Assam Breeds jk-i-vk-la-C;wjks 625 31 18 41 90 40 41 33 86 40 8 15 39 45 12 40 31 15 Number NBAGR 16104 16390 17500 17461 17323 17480 17466 16981 16821 8281 16806 17117 17436 11468 17095 16486 13401 SNP pairs Linkage disequilibrium inIndian disequilibrium buffaloes Linkage Research Accomplishments 0-5Kb 0.36 0.38 0.34 0.34 0.32 0.33 0.32 0.34 0.35 0.36 0.48 0.36 0.38 0.34 0.41 0.35 0.33 0.36 Average r

A 2 19017 17647 19747 19712 19058 19031 19703 19733 18871 8672 18984 19346 19680 12693 19321 18563 14856 SNP pairs on their ancestry coefficients.on ancestry their groups. Thus,there groupswere 11 all in based groups and rest of UP buffaloes the formedtwo groups. Tarai and Bhadawari form two separate Kalasthi buffaloes formingseparate clusters or KeralaToda buffaloes, buffaloes. Banni and fourth group of Surti, Mehsana, Nagpuri and group of Murrah and Nilithe buffaloes, Ravi second group third the buffaloes, of Chilika North region East of Assam and Manipur, the The first group consists ofswamp of buffaloes eleven different entities. breeds of buffalogroupedpopulations the all into spatial genetic analysis of SNP the data on diverse are crosses of riverine x swamp buffaloes. The therecharacteristics, are still few animals that samplingthe was done purely on phenotypic Assam are primarily swampAlthough buffaloes. 5-10Kb 0.33 0.35 0.31 0.31 0.29 0.30 0.29 0.31 0.32 0.33 0.46 0.33 0.35 0.30 0.38 0.32 0.30 0.34 Average r 2 22801 22078 23679 23645 23440 21657 23620 23661 22898 10402 22076 23250 23604 14828 23211 22190 17400 SNP pairs 10-20Kb 0.29 0.31 0.27 0.27 0.25 0.26 0.25 0.27 0.27 0.29 0.42 0.30 0.31 0.26 0.34 0.28 0.26 0.30 Average r 2 on data whichthe was generated. The technique SNPs may not common be individuals the inall nature to very the Owing of technique, the the to genomeutilized the identify markers. wide Genotyping by was Sequencing (GBS)method markers which were genome in nature. wide 12 sires was analyzed using SNP identified fieldthe belongedand thedaughters to theof The phenotypedata which was recordedfrom association studies inbuffalo: Genome wide 12= UPbuffaloes, 14= 13= Surti, Tarai, 15= 16= Toda, Assam, 17= Manipuri 1= Banni, 2=Bhadawari, 3=Chilika, 4=Murrah, 5=Jaffarabadi, 6=Kalasthi, 7=Kerala, 8= Mehsana, 9= 10= Nagpuri, NiliRavi, 11= Pandharpuri, Research Accomplishments NBAGR Estimation of admixture in proportion buffaloesusing by coufficientsancestry Admixture among diverse buffalo populations of India Data on was obtained 1763buffaloes with out versioncarried using 4.0 software. Beagle were they which The imputation born. was were imputed with sires’ the sequence data to at sequenced also 10Xcoverage. Thedaughters sires daughters to these which belonged were outwas carried using as sib half the design phenotypic data was generated. Imputation genotyped across 1763 buffaloes forwhichthe of imputation to was get SNPs used the all jk-i-vk-la-C;wjks

35 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR 36 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR the field.the traits The economic of interest were : for phenotype the which data was generated in from GBS and imputation) and traits several with known genotypesbuffaloes (obtained Thedata was analyzed usingwith CMLM 1763 reduction incomputational time. provide accurate predictions with adramatic for genomic prediction and and selection, can developed for GWAS efficiently can be used software CMLMmethod utilizes the which incorporated intoprograms. selection Gapit in large ruminants and potential this can be ultimate goals of genetic researchers especially genetic potential of individuals is one of the on genotype the based only. Identifying the prediction of breeding value of individuals estimating effect the eachof SNP foris used of SNP markers for quantitative the traits and Infinitesimal model by includinglarge number associated traits with the of interest. More so, the to SNPs the identify were which significantly forused GWAS. the The GWAS was utilized (MLM). Compressed mixed linear was model variance components mixed inthe linear model to reduce computational estimating when time EMMA Rpackage EMMAalgorithm the uses Population parameters previously defined. The Model Association (EMMA)Rpackage, P3D- package Efficient utilises which was used Mixed 1253973 SNPs genotyped. Gapit software, an R jk-i-vk-la-C;wjks Principal component analysis for milk yield NBAGR Research Accomplishments The negative logarithms the of fromP- values population structure and relatedness. familial how inGWAS well used model the accounts for yield in buffaloes presentsthe assessment as to The quantile-quantile plot (QQ) fortraitthe milk representative trait present inthe analysis. traitthe yieldis which shown being milk as the Three dimensional PCA diagram is depicted for power compared to population structure only. population structure, it improves statistical the kinshipbased matrix jointly (K) is used with individuals. the between When agenetic marker- in MLM as variance-covariance the matrix through kinship the (K) matrix, iswhich used information about relationships was conveyed about relationships among individuals. This abilityMLM the to incorporate information individuals as randomwhich effects provides and fixed both random We effects. included The mixed linear (MLM)included model analysis to care take of of structuring the data. SNP markers and Principal the used Component We Kinship the utilized matrix obtained from the (Synthetic variable). traits), Total fat (synthetic variable), Total protein at firstcalving and age at firstheat (functional Somatic count cell (as indicator of mastitis), Age yield,Milk Fat percentage, Protein percentage, Quantile-quantile plot of P-values (QQ) for milk yield Y-axis is negative the logarithm of P-value the genomicthe position of SNP, each and the summarizes GWAS results. The X-axis is The Manhattan plot is ascatter plot that association SNP the between and trait. the for QQ-plot under the null the hypothesis of no dotted lines show 95%confidence the interval P of the negative 10logarithm observed base expected logarithm of the The Y-axis negative 10 is observed the base size file graph.thethe of P-values (i.e. less significant P-values) to reduce plots inGAPIT show only asubset of larger the with trait the under study. By default, QQ- the diagonal. These SNPs are most likely associated upper of right section graph the deviate from the associations. It that SNPs isthe expected on the notdoes sufficiently account these for spurious relatedness,familial and that GWAS the model associations due to population structure and from line this suggest presence the of spurious plot should lieon diagonal the line. Deviations trait.the majority the of points the QQ- inthe SNPs tested are probably not associated with of no association trait. with the Most of the value under null the hypothesisexpected their modelsthe fitted inGWAS are plotted against Research Accomplishments -values follow auniform [0,1] distribution. The P- values under assumption the that the NBAGR P-values, and X-axis the is the Manhattan plot for in buffaloes milk yield ruminants (Cattle). iswhich aroutine inadvanced countries for large group, a procedure known as Genomic Selection from known genotypes the be shall as inference animals phenotypes predicted whose be shall genotypes and phenotypes available) and the development of areference family (with both an early stage. However, require shall this the of individuals the withgenetic higher merit at generatedcan be at allowing birth, selection the knowledge of genotypes. the These genotypes value of trait the estimated can be by only the Once SNP the effects are available,the predicted estimates of on trait the SNP based effects. Prediction Unbiased Linear and Best the We estimated Linear Unbiased Best the metacentric chromosomes. chromosomes are as wider they being large and larger Y-coordinate value. The size the firstof with stronger associations with trait the have a chromosomeEach is colored differently. SNPs for each chromosome drawn. can be entire genome and individual Manhattan plots association with trait. the Manhattan plot for the surroundingthe genomic region has a strong Manhattan plot (i.e., “skyscrapers”) suggest that SNP the between and trait). in the Large peaks from F-test the for testing H0:No association obtained from GWAS the (specifically model jk-i-vk-la-C;wjks

37 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR 38 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR 10 at taken The level significance has been p< during three the lactation stages. The marker genesthe were which differentially expressed The mappeddata then was processed to find out sequence is availablewhose inpublic domain). on cattle the genome nearest (the relative of cattle The GenerationNext sequence data was mapped lactation) and last three months (late lactation). months (early lactation), next three months (mid stages of lactationviz; inbuffaloes three Initial transcriptomeMilk data was generated for three Transcriptome inbuffalo: analysis of milk nearest genes identified. be shall of intergenic SNPs (significantly theassociated), exonic,-whether intronic or intergenic. In case annotated for identification the their locationof Trait Age at first heat Age at firstcalving Total protein Total fat Somatic count cell Protein percent Fat percent yield Milk -5 Principal component analysis -sample Folds Log - jk-i-vk-la-C;wjks . The highly significant. The highly SNPs are being SNPs various association with traits Different stages comparison NBAGR 110 140 169 198 107 208 147 132 significant SNPs Number of Research Accomplishments the microRNAsthe as well as transcription factor identified been and our includesanalysisalso and II and between I stage of lactation have also regulation of genes III and between I lactation transcripts out. carried was also The up- In addition, novo de formation of milk. compositionassociated milk with the and or Pathways the All are affected inone way Zscorethe utilising Wiki pathways. binding sites reveal following the structure as per up of genes, microRNA and transcription factor binding sites have completed. been also The sum upregulated micro RNA and transcription factor in IIIvsIIquarter. Similarly comparison the of myometrial relaxaion and contraction pathways (Wp3233)disease for IIvsIstage of lactation, autophagy, glomerular segmental focal primary III and I(WP3231)related and to senescence stage of lactation. The upregulated between genes induced circardian regulation (WP3230) for II vs I Signaling Pathway (3141)for IIvsI,exercise Cardiacin the (WP1043)for IIIvsII,BDNF III and I stage of lactation, regulation Calcium MAPK Signalling Pathway the (WP998)between The majorpathways of up regulated genes were were associated with different Wiki Pathways. of total the variance. The up-regulated genes secondvariance the PCaccounts while for 26% that first the PCaccounts for 37% the of total The principal component analysis revealed grouped together as given. data that revealed three stages the of lactation expressed. The principal component analysis of lactation present of set genes being specific The diagram reveals that three the stages of depicted. gene correlations found analysis inthe have been assembly of buffalo the carried outcarried intriplicate. Maximum yield of commercial experiments the kit. were All and 3) Combination of Trizol and method method, 2)Commercial RNA isolation kit three different 1) methods: Trizol extraction differentsources namely and milk MFG using centrifugation. RNA was isolated from two the (containing MFGs)was separated from by milk from fat buffalo milk was optimized. fat Milk for of extraction methodology the total RNA of total RNA from Fat Milk Globules (MFG), commercial kits are available for extraction of RNA from milk: buffalo Evaluation fat of as an milk alternative source buffaloes. may candidate the be genes for traits milk in QTLthe regions are which recognised being outis carried being and genes the underlying regulation of genes and associated pathways binding sites. Similarly, work the on down- Research Accomplishments NBAGR Network analysis IIand of the up-regulated Istage genes between of lactation

Since no specific mammary genesmammary expression. from buffalo MFG was suitable for studies of This study confirmed that the RNA obtained of MFG as an alternate source of total RNA. and fat derived RNA validated efficiency the fromgenes gland specific mammary milk skim and CSN2 genes. Quantitative profiling of using specific primers the for RPS9, ACTB cDNA derived from and milk fat both RNA, quantitative-PCR (qPCR)was performed on The 1.9 and cases. between to 2.05 in all be comparable. The A260/280ratio was observed integrity of samples the from was methods both as wellmethod as commercial kits. and Quality fatmilk as compared with to manual skim milk, much yields higher were obtained from buffalo isolated using only commercial kits. However, and milk fat.both amount Least of mRNA was followed by purification usingfilter columns in total RNA was obtained by Trizol method jk-i-vk-la-C;wjks

39 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR 40 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR on differentcattle types and Murrah buffaloes. Temperaturehigher Humidity Index (THI) order effect to the of visualize heat stress at These measurementsseason. were done in across hot summer, humid, winter and spring Frisian (Exotic cattle) and Murrah buffaloes cattle), Karan Fries (Cross-bred) ,Holstein in animals of Sahiwal, Tharparkar, Gir (native temp,rectal skintemperature were recorded with to respect respiration rate, pulse rate, year,the a total of 2400 physiological data and molecular changes inheat stress:and molecular Phenomic data related to cellular, biochemical Buffaloes and Adaptation inNative Cattle and Genome Data Mining for Thermotolerance On combining physiological the values, across Pulse ratePulse (PR) in Gir (GIC), Tharpakar (THC),sahiwl (SAC), Karan (KFC)and Fries Holstein Frsian (HFC) Rectal temperature (RT), Respiration rate (RR)and Temp (°F) bpm bpm jk-i-vk-la-C;wjks 100.0 100.5 101.0 101.5 102.0 102.5 103.0 103.5 98.0 98.5 99.0 99.5 cattle during hot summer (THI>85; *<0.05). 10 20 30 40 20 40 60 80 0 0 GIC GIC GIC * * * PR duirng hot summer RR duirng hot summer hot duirng RR RT in hot summer THC THC THC * * * SAC SAC SAC * * * NBAGR KFC KFC KFC * * * HFC HFC HFC * * Research Accomplishments * After Noon After Morning After Noon After Morning After Noon After Morning During During physiological stress at THI. higher nativevalues while cattle, showed minimal Karan Fries cattle showed physiological higher summer afternoon. Holsten Frisian followed by temp, skintemperature were recorded maximum breeds and that seasons, it was observed For haematological profile,data on as to conduct biochemical analysis. serum complete count profile blood (CBC) as well from breedscollected these to generate morethis, than samples 1000 blood were Holstein Frisian and Murrah For . buffaloes in Sahiwal, Tharparkar, Gir, Karan Fries , biochemical, and hematological changes parameters, efforts were made also to generate, data related to thermo-physiological In addition to generate systematic phenotype 80 PBMCs samples during collected different of THIand For season. study, this atotal of of differentcattle across degree types varying and oxidative stress were generated inPBMcs Thedata on cellularparameters like aptoptosis four differentseasons, covering different THI. in differentcattle types and across buffaloes physiological, biochemical, hematological data effort has helped to generate systematicdata on difference was non-significant (p>0.05). level during summer and humid seasons but the native breeds as well there was areduction inHb in comparison to winter In Gir period. and other during summer and humid seasons (≥85THI) showed significant (p<0.05)decline inHblevel Karanboth Fries and Holstein Frisian cattle parameters in different breeds. For example, showed effect hematologicalseveral season on to differentexposed seasons and THI. The data generated in5cattle and 1buffalo breeds (Hb), packed volume cell etc. (PCV), were hemoglobin red cells (RBCs), blood (WBCs), hematological parameters like white cells blood respiration rate, pulse rate, rectal Such an various in hot was observed to be predominantly to be was observed present in (n=95), and Karan Fries genotype (n=150).CC crossbred cattle- Jersey (n=36), Holstein Frisian (n=30),Gaolao (n=20)and exotic/ Ladakhi Nimari(n=32), (n=30), Dangi (n=26),Khillar (n=99), Hariana Kandhari(n=32), (n=32),Red (n=32), Kankrej (n=30),Gir(n=59), Sahiwal (n=32), Nagori Sindhi (n=32),Malvi (n=32),Red Indianviz., cattle- TharparkarRathi (n=84), screened for HSF-909 from locus 18cattle breeds 909andlocus: 4693.Atotal of 883animals were and genotyping out was carried at two different characterizedsequenced in Indian native cattle genes, year, this heat shock factor gene was earlier effort to characterize, heat responsive agroclimatic regions: nativediverse cattle adapted to different factor inheat shock gene in spectra Allelic in hot summer as well. are to generate underway cellular response data in comparison to PBMCs of native cattle. Efforts of Karan Fries cows during hot humid condition stress indicated cellular higher stress inPBMCs The meandata on total apoptosis and oxidative stress at THI on higher differentcattle types. were done inorder to effect the visualize of heat (August 2017-March 2018).These measurements across hot humid, winter and spring period Gir, Sahiwal, Tharpakar and Karan cattle Fries torespect total apoptosis and oxidative stress in seasons were to generate used cellular data with Research Accomplishments of Sahiwal (SAC), Tharparkar (THC), Gir (GIC) and Comparative analysis of cellular aptoptsis in PBMCs

Karan (KFC)cows across Fries humid, winter and Total Apoptosis (%) 10 20 30 0 a NBAGR SAC b a Apoptosis a THC b spring seasons b a In continuation to our GIC b b a KFC b b Spring Winter Humid 40 (SOD2) peroxidase-1 (GPX-1),superoxide dismutase 2 1 factor A(VEGFA transporter-1), glucose (GLUT- factor endothelial α(HIF-1α growth), vascular hypoxia related genes such as hypoxia inducing made to evaluate transcriptional the pattern of tropical condition: adaptation cows from migrated injersey Expression of genes related to altitude high studies for theromotolerant and adaptation traits. forvital future genotype: phenotype association and GTgenotype. Such information be will cattle of with frequency 0.725followed by GG genotype was predominantly present inIndian Karan Fries (n=99).Analysis that revealed TT Jersey(n=14), Holstein Frisian (n=48),and (n=30) and exotic/crossbredLadakhi cattle- Nimari(n=32), (n=30), Dangi (n=26),Khillar (n=71), Hariana Kandhari (n=32),Red (n=32), (n=32), Kankrej (n=30),Gir(n=63), Sahiwal Nagori Sindhi (n=32),Malvi (n=32),Red Indian cattle- TharparkarRathi (n=32), (n=82), animals were screened from 17cattle breeds Additionally, for HSF-4693 atotal of locus, 711 0.148 frequency, respectively. followed genotype by with 0.237, and CC TT TC genotype of with was frequency higher 0.615 Whereas inexotic or crossbred cattle breeds the (0.895) followed by genotypes. TC and TT Indian cattle breeds with overall frequency allelic level) and at then regular ( 2 intervals at 8differentpointstime i.e. from jugular external vein of 18 Jersey animals condition. For study this was collected blood level tropical condition altitude to high hypoxia analysis has helped us to understand about the for expressionused qPCRbased analysis. The mononuclear cells (PBMC) were purified and altitude.shifted to high peripheral The blood ), Tumor necrosis factor-1 (TNF-1), glutathione th , 80 th etc etc , 86 in Jersey cattle transported from sea th , and 110 In study, this an effort was th week) after animals were zero week (mean sea jk-i-vk-la-C;wjks nd , 3 rd , 23 rd ,

41 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR 42 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR (p<0.05) inheat stressed fibroblasts cells. oxygen and species ΔΨm increased significantly cytotoxicity, apoptosis, production of reactive otherthe hand, cellular parameters like cell significantly (p<0.05) On fortheboth species. rate of heat stressed fibroblast cells decreased determined.viability The cell and proliferation of heat responsive genes and miRNAs were oxidative stress, along with expression pattern mitochondrial membrane potential (ΔΨm), cellular parameters., apoptosis, viz proliferation, and 24h) along with control samples. Different different (0h,2h,4h,8h,16h intervals time allowed to recover at 37°C and at harvested at 42°Cfor 1hour. The cells were subsequently ( indicus cowsSahiwal (Bos culture established form ear pinna samples of and buffaloes: fibroblast skin stressed of Indian nativecattle and transcriptional changes inheatCellular molecular tools. altitudehigh acclimation and adaptation using are to generate underway more information on associated altitude with high adaptation. Efforts expression pattern of genes certain known to be Bubalus Bubalis Bubalus jk-i-vk-la-C;wjks For study, this fibroblast primary ) were exposed to thermal stress) were to thermal exposed Gene ontology catgoriesGene of DE in Bandur genes and Local sheep of Karnataka NBAGR ) and Murrah buffaloes Research Accomplishments Th e native cows compared to riverine buffaloes. tolerance of fibroblastfrom cells derived Indian and after heat stress indicatedbetter cellular HSP (Fig.3). level inbuffalo and nativecattle fibroblast cells subtle differences ininduction their expressionof during pre- and post-heat stress showed also immune genes and heat responsive miRNA pattern heat shock proteins, inflammation/ expressed across Bandur sheep. and local Of transcripts differentially to were be observed sequence assembly Oar 3.1. were filtered and mapped the to sheep genome on Illumina Platform. HiSeq-2000 The raw reads Karnataka. of skeletal muscles of Bandur sheep of and local to establishwas used transcriptomic the profiles throughput The high RNAsequencing technique acid and histidine as compared sheep. to local Bandur had asignificantly level higher of oleic analyzed for 17amino acids and 12fatty acids. The mutton of Bandur sheep and was local of Bandur (Mandya) Sheep MuscleSkeletal Transcriptome Profiling genes, and heat responsive miRNA before Thekind of cellular response,pattern of Paired end sequencing was performed A total of 20568 The datalarge a to identify used also was number and differentiation and Ketone body metabolism. pathway, Lipid metabolism, Muscle development Biosynthesis of amino acids, signaling Calcium pathway,signaling PPAR pathway, signaling were AMPK signaling pathway, Adipocytokine were that detected are relevant to meat quality protein and fat metabolism. Prominent pathways 99GOterms wereexpected, related to muscle or as well as amino acidphosphorylation. As regulation, and actin cytoskeleton organization, polymerization, muscle development and cellular response to stress, regulation of protein nucleic acidbinding, RNA catabolic process, significantly enriched clusters were involved with enrichment score of > 0.76 and p<0.05. The of 25annotation clusters were identifiedwith and 117terms for molecular functions. Atotal process, 146 terms for cellular components OntologyGene (GO)terms for biological categories using DAVID included which 132 genes classified could into be functional 395 out of which 602 were significant.the All DE Similarly, 9839 transcripts were up regulated, significantly down regulatedBandur in (p≤0.05). 10729werethese, down regulated and 1236were Research Accomplishments Electrophoretic obtained patterns ed by tetra-primer ARMSPCRb. by a.SNP1 genotyping: genotyp SNP2 by PCR- RFLP with Hpy CH4 III restriction enzyme. c. Hpy SNP4RFLP with byd. allele SNP3 by PCR- enzyme. specific PCR. with CH4IIIrestriction RFLP Taq αI NBAGR -primer ARMS PCR. e.SNP5 by tetra ARMSPCR. -primer enzyme. restriction lymphocyte (N/L) ratio, and total leukocyte total and ratio, (N/L) lymphocyte neutrophil/ (TEC), count erythrocyte total volume, cell packed Hb, like parameters rate (a.m. and p.m.), as well as hematological rateandpulsei.e., temperature,respiratory and breed on all the physiological parameters, revealed significant effect (P which out carried was analysis square Least calculated. was percentage adaptability their under normal and heat stressed condition and both generated was parameter biochemical hemato-physio- each for breeds the all for values reference The breeds. sheep Red Madras and Marwari, Magra, Chokla, supported bytheirexpressionprofilesin with hemato-physio-biochemical parameters, HSP70 gene polymorphism and its association thermo-tolerance is modulated by HSP90 and how study to conducted was research The Evaluation for Heat Stress inIndian Sheep and Physio-biochemicalMolecular in meat quality of Bandur sheep. and local and pathways that contribute to differences the results provide information of candidate genes of SNPs across two the groups of sheep.The

< 0.05) of season jk-i-vk-la-C;wjks

43 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR 44 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR significantly effected (P<0.05) in case of case in (P<0.05) effected significantly were SNP2 and SNP1 of genotypes the and THI)(low season winter during values mean (P<0.05) higher significantly had T4 and T3 SNPs of HSP90AA1 and HSP70 genes. Both identified 5 and season breed, among level cortisol of effect (P<0.05) significant no was There (65). THI lower than condition (84) THI high in higher be to found was level cortisol The ELISA. by level T4 and T3 breed were used for the estimation of cortisol, Red Madras and Magra Marwari, Chokla, of season) per and breed per animals (10 to heatstress. A totalof80plasmasamples adaptable more animals of identification the for advantage selection of be to found was SNP1-SNP2-SNP3-SNP4-SNP5 of alleles ofTACCA selection). combination (positive manner directional in acting selection the to be superior in most of the SNPs indicating found was allele Predominant SNP5. at TLC andSNP4 at N/Lratio and (g/dL) Hb (p.m.), SNP3; and rectal temperature (p.m.), pulse rate SNP01; albumin at SNP2; pulse rate (p.m.) at triglyceride, and total protein was observed at (p.m.), rate respiratory (a.m.), temperature A significant difference (P difference significant A carried out for 50 DNA samples of each breed. was SNPs five of genotyping large-scale and developed were protocols genotyping PCR allele-specific PCR-RFLP,and PCR, system Tetra-primer amplification refractory mutation differentamong conserved species.livestock fragments revealed that these genes are highly gene (96%) HSP70 and (93.6%) 90 HSP of (SNPs) were identified. polymorphisms single-nucleotide 13 and genes were characterized by direct sequencing 70 and HSP90 cholesterol. and triglyceride, phosphorous, SGOT, glucose, as such (P count (TLC). There was a significant influence

< jk-i-vk-la-C;wjks 0.05) of breed on biochemical parameters NBAGR Phylogenetic analysis

< Research Accomplishments 0.05) of rectal of 0.05) native animalsduringperiodsofstress. adaptable more in observed was expression cattle and buffalo breeds in which lower HSP different in generated genes HSP different of profiles expression the with consonance expression pattern. Marwari < Chokla < Magra < Red Madras higher expression in less adapted animals with revealed analysis expression Gene only. T3 and C-CMotif Chemokine Ligand 5(CCL5; ligand16 (CXCL16; FPKM T1:116.349 ) motif) of chemokines such as chemokine (C-X-C in animals Increased after trial. expression L (ITGAL) ), Integrin), cathepsin S(CTSS W (CTSW alpha superfamily member 1B (TNFRSF1B), cathepsin DRB, DQA, CCL5, MHCB3, TNFreceptor immuneSeveral related genes such as T1 and T2groups incomparison to T0group. system showed also an increased expression in presentation of antigens peptide to immune the (B2M), an immune related gene involved inthe top up-regulated genes. Beta-2-Microglobulin (RPL31) (RPL27A), (RPL37), L35A (RPL35A), L34 ( RPL34), S13 (RPS13),S6RPS6S24(RPS24),L37 S21 (RPS21),S15A(RPS15A),S28RPS28), protein (T2).Ribosomal hr post-trial such as and after endurance (T1) exerciseand trial 2 expressed inZanskar ponies before (T0) trial total of 646genes were found differentially to be exercise at altitude high among Zanskar ponies. A or down-regulated before and after endurance groups) was generated to genes the identify up- (T2)exercise (T1)and recovery period post samples (3 each from pre-exercise (T0), post- RNA transciptome based seq data of 9PBMCs atEndurance High Altitude of Ladakh Evaluation Ponies of Zanskar for and L5(RPL5)genes were among the were found also up-regulated to be L35 (RPL35), L23(RPL23),L31 The resultsobtainedare CXCL16, L27A

pathways impacted were glucagon signaling (21 genes). Some of important the molecular lipid binding (23genes), and transferase activity genes), transducer molecular (24genes), activity activity (33genes), enzyme regulator activity (32 (65 genes), ion binding (55genes), transporter hydrolase activity (73genes), nucleotide binding activity (93genes),genes), molecule structural binding (343genes), nucleic acidbinding (190 molecular functions identifiedwere; protein and proliferation cell (65 genes). Similarly, major (159 genes), communication cell (157genes), process (164 genes), multi-organism process stimulus (216 genes), unicellular organismal genes), localization (244 genes), response to process (311genes), biological regulation (276 data Top set. biological processes were metabolic processes and functions molecular inthe enriched classified in terms of most significant biological The up-regulatedZanskar genes in ponies were expression inT1and T2groups after exercise. dismutase 1(SOD1 showed) also increased 1A ( significant induction of hypoxia inducible factor strong immune response. Data suggested participate regulation cycle incell and induce stress conditions. These chemokines actively strong inflammatory response in horses during FPKMtreatment T2:3604)aftertrial indicated Research Accomplishments endurance trial (T0: Before exercise, T1:immediatelyendurance (T0: trial exercise, Before expressed genes (DEGs) genes expressed in Zanskar ponies under Up-regulated and differentially down-regulated HIF-1A). The anti-inflammatory superoxide NBAGR after T2:2 trial; hr post-trial) cytotoxicity, RNA transport and protein export. and presentation, natural mediated cell killer receptor signaling pathway, antigen processing pathway, NF-kappa Bsignaling pathway, Bcell and 0.3073;0.2831and 0.2817, respectively. 0.3362 and 0.2981;0.3435and 0.2992;0.3136 heterozygosity and expected observed were Indianin the Corresponding yaks. values for INDELs were identifiedwith a coverage of 9.42% of quality 5,79,575 high SNPs along with 50,319 49,665 and 33,782, respectively. a total Overall, and at yaks Sikkimi RD 10 were 1,04,188, 67,032, genotyped inArunachali, Himachali, Ladakhi 2000platform.HiSeq The number of SNPs using Illumina TrueSeq on Illumina chemistry identification of genomewide SNPs and INDELs were and yak for Sikkimi used theLadakhi Indian belonging yaks to Arunachali, Himachali, Genome Wide Diversity in Indian Yaks Variants identified in Indianyaks (RD10) Top for molecular ontology functions terms jk-i-vk-la-C;wjks

45 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR 46 ANNUAL REPORT 2017-18 Genomics & Evaluation of AnGR Genetic Defects inIndian Defects Livestock Genetic Karyotyping and DNA Testing for F The lethal mutationlethal at GART gene, causing the 40 Jersey bulls a screened. The HH4is also heterozygous condition animals in seven from presenceconfirmed the of with JH1 allele Sequencing and PCR-RFLP by TaqαI enzyme embryonic death of foetus inJersey cattle. a point mutation inCWC15 gene causes early respectively. JH1,originated Lethal through in Jersey and Holstein genetic groups, heterozygous condition were identified Two mutant -JH1 and alleles HH4 in samples ineach group. and indicus indigenous Bos Crossbred, Holstein Frisian (HF),HFCrossbreds in five different genetic groups- Jersey, Jersey were disease each amplified and sequenced in Indian cattle population. PCRproducts for characterized for presence the of mutant allele(s) Holstein cholesterol (HCD) – deficiency were Holstein(JH1), haplotypes -HH3,HH4and vertebral malformation (CVM),Jersey Haplotype Congenital of – fiveLoci diseases lethal genetic yak populationsyak are distinct. Indian and Sikkimi that revealed yaks Ladakhi distance on genome based SNPs wide inthe and The yaks. 0.015 genetic in Sikkimi in Ladakhi Himachali whereas yaks, it was found 0.022 to be jk-i-vk-la-C;wjks PCR-RFLP Test for JH1mutation bull in Jersey IS value was 0 in case ofvalue was 0incase and Arunachali NBAGR comprising 10male Research Accomplishments project during period. the institute provided under from the services these Rs. 8,30,500/- was received as a revenue to the found chromosomal the to carry Total defect. were for screened One bull was genetic diseases. Total 142bulls of cattle (93)and buffalo (49) dogs were for screened chromosomal defects. buffalo female, 17 breeding pigs and 5 breeding 344 bulls/males (125 cattle, 219 buffalo), one Rajasthan &UPstates and Indian Army. Total of J&K, HP, Uttarakhand, Punjab, Haryana, was provided to different government agencies phosphate synthase (DUMPS, in HF/HFX only) and of uridine monoCitrullinemia Deficiency testing for BLAD, genetic diseases- FXID, for chromosomalServices screening and DNA of exotic origin. in Indiangenetic diseases cattle, specifically would helpful be for rapid screening of the mutations. tests developed PCRbased These developed for screening of JH1and HH4 tests including PCR-RFLP protocols were inexotic Novel type. specifically PCR based HH4 mutations inIndian cattle population, reports about presence the of JH1and lethal (HF and HFcrosses) screened. are These first two out males of 100 bulls of Holstein origin indicated presence the of HH4 mutant in allele Sequencing and PCR-RFLP by enzyme MseI embryonic death incows of Holstein origin. Chromatogram homozygous (normal) and of wild Heterozygous (carrier) animalHeterozygous (carrier) for JH1loc plan, project were activities shifted to buffalo plan by establishing 3core labs. During characterization commenced was also in IX along with characterization of breeds. Genetic plan, conservation were activities initiated also characterization of breeds. IXand During X Project was initiated in VIII plan for Network onAnGR Project Research Accomplishments 8. Conservation 7. 6. 5. 4. 3. 2. 1. Characterization No. Sr. 9. Livestock and poultry breeds for breeds characterization and poultry Livestock Network under and conservation Project NBAGR Camel Camel Camel Chicken Duck Goat Cattle Species Chicken Marwari and Sindhi Poonchi Manipuri Sojat Balangir Kathani Kamma Vandarvi Breed/population Kharai Ankleshwar

XI XI  breeds are habitat. intheir conserved being have for undertaken characterization been and 2 conservation. In populations 2017-20, 9 new for characterization and 4at risk breeds for breeds/populationsnew have undertaken been shifted basic to mandate the of Bureau and 18 buffalo genome. In XII plan, was againfocus genomics to establish information baseline of Sahjeevan (NGO) NRCC, Bikaner SKUAST, Jammu CAU, Imphal RAJUVAS, Udaipur BAIF, Pune WASSAN (NGO), Telengana Centre AAU, Anand, Gujarat jk-i-vk-la-C;wjks

47 ANNUAL REPORT 2017-18 Network Project on AnGR 48 ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks NBAGR Research Projects &Publications Awards • Publications • Research Projects • NBAGR jk-i-vk-la-C;wjks

49 ANNUAL REPORT 2017-18 50 ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks NBAGR 6. 5. 4. 3. 2. 1. IRCCompleted Projects Research Projects RESEARCH PROJECTS &PUBLICATIONS March, 2018. Sharma (wef. 01.04.2017)-April, 2016 to & HMYatish (KVAFSU, Bidar) and Rekha Kulkarni (UAS, and Dharwar) VBShettar goats of Karnataka -MSTantia, PK Vij, VS Characterization of Bidri and Nandidurga 2018. Ganguly, LUVAS -April, 2014to March, Venkataramanan (TANVASU) and Anita Ganguly, HKNarula KNRaja, (CSWRI)R Indian sheep breeds - Sanjeev Singh, Indrajit evaluation of Heat Stress Protein genes in Molecular and physio-biochemical 2016 to March, 2018. Kumar Yadav and Avnish Kumar - April, classical and -Dinesh heuristicmethods India on morphometric based traits using Classification of indigenous sheep breeds of April, 2015to March 2018. Mishra, Sanjeev Singh and Anand Jain - and Dumba sheep native intheir -AK tract Phenotypic characterization of Chitarangi 2014 to March, 2018. Mishra and SK (OUAT, Das Odisha) -April, SK Niranjan, Vikas Vohra (NBAGR), Sanat populationsbuffalo Odishaof -RS Kataria, Phenotypic and genetic characterization of 2018. 2014 to March, 2017,extended up to March, Indrajit Ganguly and Sanjeev Singh -April, on Y-Chromosomebased markers specific - Haplotypic diversity of Indian cattle breeds NBAGR 7. 6. 5. 4. 3. 2. 1. Ongoing IRC Projects in Indian Cattle -Karan Veer Singh, Vikas variation associated with tick infestation Identification of genetic and phenotypic 2015 to March, 2020. PK Singh, AKMishra and -April, KNRaja at Gaushalas -RKPundir, Arjava Sharma, ofConservation and Sahiwal Hariana cattle to March, 2020. HK Singh (CHRSRohtak) -October, 2017 West Rajasthan -Anand Jain, DK Yadav and populations of Haryana, Punjab and North- evaluation of mixed indigenous cattle Identification, characterization and 2019. Foundation - October, 2017 to September, MS Tantia and Sukram Ninama; Prakriti cattle of Gujarat PK Vij, Aggarwal, -RAK Characterization of Mangadh Dahodi 2017 to September, 2020. and Kumar KAnil (KANVAS) -October, AKMishra,Raja, RSaravanan (TANVAS) population of Kerala and Tamil Nadu -KN Characterization of cattle non-described NGO) -July, 2017to June, 2020. (TSBDB) and Sabyasachi (WASSAN- Das PK Singh and PSDangi, Shilpi Sharma and Kamma) of Telangana state -RKPundir, known cattle populations (Vandharvi, Zari Characterization and evaluation of lesser April, 2014to March, 2018. (NDRI) and Arora Reena (from Nov.2014) - Ahlawat, Sharma Rekha and Sachinandan De sub-fertility or sterility inbovines -Sonika Deciphering molecular the basis of male jk-i-vk-la-C;wjks

51 ANNUAL REPORT 2017-18 RESEARCH PROJECTS 52 ANNUAL REPORT 2017-18 RESEARCH PROJECTS 14. 13. 12. 11. 10. 9. 8. jk-i-vk-la-C;wjks animal genetic resources of India -Avnish Development of mobile application on farm 2017 to March, 2020. andLeh) Vijay KBharti (DIHAR, -July, Leh) Jyotsna Behl, Rahul MIqbal Behl, (CAHO, Himalayan region of India -SK Niranjan, andyak donkey populations of North trans- Phenotypic and genetic characterization of 2018 (Extended). Karan Veer Singh - April, 2015to March, Yak populations -Jayakumar S,SP Dixit and Genome diversity wide analysis of Indian April, 2015to March, 2018(Extended). Class IIantigens -Jyotsna and NKVerma - goatthe Major Histocompatibility complex Study of genetic the variability present across March, 2020. Sharma and PSDangi -October, 2017to states -NKVerma, Rekha Aggarwal, RAK germplasm of Andhra Pradesh and Telangana Identification and characterization of goat to March, 2019. AhlawatSonika and RKVijh -April, 2016 Bandur (Mandya) Arora, sheep -Reena Skeletal muscle transcriptome profiling of September, 2020. (VO- ARD, Tirpura) - October, 2017 to SV Kuralkar (PGIVAS, Akola) and R Das Monika Karanvir Sodhi, Singh, PSDangi, and eastern India -Vikas Vohra, RSKataria, non-descriptpopulations buffalo of central Characterization and evaluation of some 2019. (BBAU Lucknow) -April, 2016to March, Vohra, Jayakumar Sand Kamal Jaiswal, RESEARCH PROJECTS &PUBLICATIONS NBAGR 1. FundedProjectsExternal 17. 16. 15. 3. 2. Avtar Singh (NDRI) - 9 Iquebal (IARI), Dinesh Kumar (IASRI) and Dixit, Jayakumar S,AKDang (NDRI), MA and indigenous dual-purpose cattle -SP development of breed signatures for dairy Whole genome SNP mining based and April, 2016to March, 2021. project) -SK Niranjan and Jayakumar S- genetic in Indian defects bovines (Service Karyotyping and DNA testing for screening June, 2019. and HK,Verma (GADVASU) -July, 2017to states -PK Singh, RKPundir, Arjava Sharma ResourcesGenetic in Haryana and Punjab programmes for management the of Animal Evaluation of livestock and policies Contd. MS Tantia (I/c,Network Project) -1992- Director NBAGR, Project coordinator and Resources - Arjava Sharma (w.e.f.01.10.2013) Network Project on Animal Genetic March, 2019. Kumar and DK Yadav -October, 2017to Institute of High Altitude Research, - Leh) (NDRI) and Vijay KBharti (Defence Niranjan and RSKataria, Ashok Mohanty region - Manishi Mukesh, Monika SK Sodhi, in Zanskar ponies adapted altitude to high pathways associated with endurance traits Identification of genes and molecular June, 2018. RS Kataria and SK Niranjan -July, 2015to - MonikaA1A2 milk M Mukesh, Sodhi, cows and potential implications health of variantsDelineating casein beta inIndian July, 2017. th July, 2014 to 8 th

1. National Fellow Project 5. 4. RESEARCH PROJECTS &PUBLICATIONS May, 2016extended up to May, 2021. - buffaloes Manishi Mukesh - May, 2011 to to diverse environment innative cattle and basis of tolerance thermo and adaptation Genome data mining to unravel molecular March, 2017,extended up to March, 2020. Jayakumar Sand SP Dixit -June, 2015to - RKVijh, Arora, Reena Ahlawat, Sonika Transcriptomics study inBuffalo and goats of markers for economic traits and project on Genomics: -Identification ICAR-AGRI Research Consortia Platform March. 2020. April, 2015to March, 2017,extended up to Vij, and Sharma Rekha Aggarwal RAK - animal genetic resources -MSTantia, PK CRP on Agro-biodiversity conservation of to 30 July, 2015to December, 2016,Extendedup NBAGR th November, 2017. 3. 2. 1. Research Articles Publications Livestock Biodiversity morphometric of characteristics. Journal of Indian donkey populations on based RK (2017) Comparison of three types Niranjan R, Behl Jand SK, Vijh Behl Sciences 87(11):1362-1366. Animal of cattle.Sahiwal Indian Journal in Toll like receptor indicus 9 gene in Bos Characterization of genetic polymorphism Kimothi SP, Bhatia R(2017) AKand Behl JD,Behl Sharma A,Kataria RS,Verma NK, Animal Sciences 87(4):525-528. mutton traits in sheep. of Indian Journal SNPs inovine GDF8 gene associated with primer ARMS-PCRassay for genotyping M, Tanwar Nand Girdhar Y(2017)Tetra- Arora Yadav R, DK, Sharma A,Pothuraju 7(1):17-21. jk-i-vk-la-C;wjks

53 ANNUAL REPORT 2017-18 PUBLICATIONS 54 ANNUAL REPORT 2017-18 PUBLICATIONS 8. 7. 6. 5. 9. 4. 10. jk-i-vk-la-C;wjks Bubalus bubalis buffalo (Bubalus its association production with milk traits inriver RS (2017)PCR-SSCP analysis gene of and MDGI J,. Singh KP, Kathiravan P, Mishra BP and Kataria S,Mishra PK, SK, Dubey AroraDubey Patel R, cattle Biotechnology breeds. Animal SNP genotyping array in indigenous dairy of High-Density Bovine (2017) Evaluation Sharma A,Singh S,Ganguly Iand Dixit SP Dash S,Singh A,Bhatia AK,Jayakumar S, Biodiversity of equines Livestock inIndia. of Journal (2017) Population trends and distribution Vij R, Behl Jand Sharma PK, Behl A Sciences 87:1221-1225. of Spiti donkeys. Indian Journal of Animal analysis for genetic diversity and bottlenecks (2017) Characterization and microsatellite J, Vijh RK,Attri PN, Nadda Sand Joshi BK J, Sadana DK, R, Behl P,Behl Banerjee Joshi of Animal Sciences 87(9):1102-1105. 1 isoform ( isoform 1 characterization(2017) Sequence of Alpha Sharma VL,Verma Pand Mukesh M Kaur M, Swami Sharma R, A, Sodhi SK, Science using microsatellite markers. of brown donkeys of type Andhra Pradesh Sharma characterization A(2017)Genetic DMV,Arora Rao R, PP, Reddy Vijh RKand Niranjan R, Behl J, SK, Behl Tantia MS, genes for normalization of quantitative of suitableM (2018)Selection reference Verma P, Swami SK, Kumari P and Mukesh M, SharmaKaur A, Sharma Sodhi VL, R, bubalis (Bubalus isoforms across tissues of riverine buffaloes and expression characteristics of its major 115: 307-309. 115:307-309. ATP1A1) of Na 7(2):71-77. RESEARCH PROJECTS &PUBLICATIONS ). Gene Reports 10:97-108. NBAGR ). Research in Veterinary + /K Indian Journal Indian Journal + ATPase gene 1-7. 11. 16. 15. 14. 13. 12. bovines. butyrophilin (BTN1A1) gene in Indian and Vohra diversity V(2017)Allelic of Kumar M,Ratwan P, Chopra R, Das A journal.pone.0191558. bubalis twelve tissues of riverine (Bubalus buffaloes RT-PCR (RT-qPCR) expression data across heat shock protein ( Beta-1 of and coding untranslated regions of OP and Mukesh M(2018)Conservation Singh MK,Kumari P, Sharma A,Verma Nigam A,Swami M,Verma SK, Sodhi P, Sciences indicus neutrophils of periparturient (Bos Sahiwal control genes incirculating poly-morpho- of stably(2018) Selection expressed internal MS, Kataria RS,Mohanty M AKand Sodhi Mukesh M,Kishore A,Sharma A, Tantia DOI:10.18805/ijar.v0iOF.8000 of India. Indian Journal of Animal Research populationsbuffalo in North-East region suggests distinctness of riverine-swamp the diversityGenetic at MHC-DRB3 locus Singh RV,R, Kumar Nand Kataria RS(2017) Mishra SK, Niranjan B, SK, Banerjee Singh Animal Sciences goats of Maharashtra state. Indian Journal of of Dangi PS and Verma NK (2017) Mishra P, Dixit SP, J, Behl RAK, Aggarwal Research population. in Indian native cattle breeds and crossbred Bhakat M(2017)Y-chromosome variation Kumar Mand S,Ganguly I,Singh S,Sodhi 22(2): 92-97. genetic diversity and relationship among Indian Journal of Animal of ) cows. Indian Journal 88:84-90. ). 51(1):1-7. Indonesian Journal of Biotechnology of Indonesian Journal PLOS ONE doi.org/10.1371/ Indian Journal of Animal of Indian Journal 87(8):982-990. HSPB1) gene Estimation

21. 20. 19. 18. 17. RESEARCH PROJECTS &PUBLICATIONS bubalis indicus of lactoferrin gene cows inSahiwal (Bos sequence variability and expression pattern P and Mukesh M(2017)Analysis of AK, Niranjan SK, Shandilya UK,Kumari Kataria RS,Jain P, Bhatia AK,Mohanty Sharma A,Swami SK, Kumar M, M,Sodhi 10.1111/rda.13187. Animals DOI: Domestic parameters. seminal varying Reproduction in methylation of DAZL gene in bulls with and Arora R(2018).Differential promoter Sarova N,Ahlawat S,Grewal A,Sharma R DOI:10.18805/ijar.v0iOF.8492. breeds. identification of TNP1gene inIndian cattle characterization(2017) Sequence and SNP Gupta ID, Bhakat Mand Mohanty TK Ranjan Ganguly KN,Sinha A,Raja R, I, v0iOF.9132. Animalof Research breed of Southern India. Indian Journal Cytogenetic profile of Rajapalayam dog PK, Mishra AK and Ganguly I (2017) KN,SaravananRaja Devendran P, R, Singh 172-176. region. ponies adapted altitude to high of Ladakh response to endurance exercise of Zanskar Evaluation of physiological parameters in A, Vivek P, Giri Aand Mukesh M(2018) VK, Kumar B, Garg A,Yadav MC, Sharma Niranjan SK, M,Bharti Kataria RS,Sodhi Gene bubalis riverine (Bubalus buffaloes Indian native indicus cattle (Bos mononuclearperipheral blood cells of and its expression pattern inheat stressed NBAGR 8:9-17. ). Journal of livestock Biodiversit Bubalus ) and Murrah (Bubalus buffaloes Indian Journal of Animal Research Defence Life Science Journal 3(2): DOI:10.18805/ijar. ). ) and and ) y 7:1 Agri Agri

28. 27. 26. 25. 24. 23. 22. stress tolerance associated HSP60 Comparative expression profiling of heat- Vohra V, Niranjan SK and Kataria RS (2018) Rajesh C, MishraSingh R, SK, Gurao A, 259-266. Research Livestock of Journal International A Distinct Registered Buffalo Breed of India. Niranjan SK and Kataria RS(2017)Chilika- MishraSingh R, SK, Rajesh C,Dash SK, Bioinformatics Proteomics MOJ Containing TCP-1 Subunit Taurus. 5inBos Insightswith Structural into Chaperonin Computational Analysis of HSP-60 Protein Sharma P, Mishra SK and Kataria RS(2017) Kulshrestha M,Kaushik A,Sehgal A, KumarSingh R, V, Rajesh C,Gurao A, 7(3):142-148. Research Livestock of Journal conditions.field International Performance of Gangatiri cattle rearing in Singh Gand PK, Sankhala Singh PK (2017) Research dromedarius gene, and genetic variability of Alpha Casein ZS (2017)Molecular characterization Singh KV, Jayakumar S,Dixit SP and Malik Chaperones aries in Indian sheep (Ovis gene polymorphism with adaptability traits Association of heat stress protein 90and 70 Ganguly A, Chopra A and Narula HK (2017). Singh KM, Singh S, Ganguly I, KN, Raja Sciences donkey of India. Indian Journal of Animal geneticreveal high diversity inRajasthan Simplesequence repeat (SSR) genotypic data P, Yash Pal, Rand Tantia Behl MS(2017). Sharma Sharma R, H,Ahlawat S,Panchal Camelus CSN1S1 inBikaneri camel (Camelus 87(12):1497-1503. DOI:10.18805/ijar.B-3468. 22:675-684. Indian Journal of Animal of Indian Journal ) milk. ). Cell Stress and 6(1):00183. jk-i-vk-la-C;wjks and GLUT- and 7: 7:

55 ANNUAL REPORT 2017-18 PUBLICATIONS 56 ANNUAL REPORT 2017-18 PUBLICATIONS 1. Technical /Popular articles 33. 32. 31. 30. 29. jk-i-vk-la-C;wjks India. PLoS ONE 12(9):e0184691. five ecotypes Deccani sheep of Maharashtra, Classification and conservation priority of Yadav DK, Arora R and Jain A (2017) Animal Sciences in Chhattisgarhi buffalo. of Indian Journal biometric traits and shared their variance Kataria RS (2017) Multivariate analysis of Vohra V, Chopra Singh R, M,Das Aand Animal Sciences 88(2):194-199. generation of Indian inbuffaloes. Journal phenotype recording and genotype data Creation of alarge reference family with Vijh RK,Sharma SB Uand (2018) Gokhle Life Science Journal 3(2):177-181. anaturalbe resource for Defence A2milk. gene establishes native cattle to of Ladakh genotyping of variants allelic casein of beta characterizationM (2018)Sequence and A, Kalia S,Gagoi D, Sarangi PP and Mukesh Kumar B, Iqbal M,Rabgais S,Kumar P, Giri P, Verma P, Swami SK, Sharma A,Bharti VK, M,Kataria RS,NiranjanSodhi SK, Kumari Animal Sciences indigenous sheep breeds. Indian Journal of its phylogenetic relationship with other diversity estimation inKajali sheep and genetic based R (2017) Microsatellite Singh Y, Singh KM,Ganguly Iand Arora Singh S,Mishra AK,Vohra V, KN, Raja Dairy Science 71:183-186. 1 i'kqèku çdk'k(8)%18&21- vè;;uA ,oa ewY;kadu dk HksaM okyh tkus ikbZ esa {ks= if'peh & mÙkj ds Hkkjr % fprjaxh (2017) flag latho ,oa tSu vkuan feJ] dqekj vfuy Indian Journal of genes of inIndian Indian Journal buffaloes. RESEARCH PROJECTS &PUBLICATIONS 87(7):864-870. 87(9):1097-1101. NBAGR 6. 5. 4. 3. 2. 11. 10. 9. 8. 7. çdk'k 8% 50&55 fodYiA mRÏ"V ,d fy;s ds vkthfodk VkafV;k ,l ,e ,oa xkS&uLyA i'kqèkuçdk'k(8)%1&5- kosk qk (2017) dqekj Hkqous'k Lokeh] dVkfj;k] flag j.kthr i'kqvksa esaXHkkZ';ksèk] nqXèkxaxk(5)%44&46- esJke] f'kogjs] iq"ijkt o mi;ksxA i'kqèku çdk'k8%28&32- y{k.k] 'kkjhfjd dk cdfj;ksa okyh ckyksa (2017) oekZ dqekj ujs'k gtjk] rUe; dj.k ohj flag ,oa ,l t;dqekj i'kqvksa dkcpkoA i'kqèkuçdk'k 8%89&90- (2017) eqds'k euh"kh ,oa dqekj vouh'k oksgjk] fodkl flag] ohj dj.k ky dej gsn fa pSk] k t cqjVs] th vkj pkSgku] flag gjsUnj dqekj] 'kkyw vgykor] lksfudk dqekj vfuy ,oa çdk'k (2017) ch feJ flag] oh lh iqaMhj] dqekj jkds'k lks<+h eksfudk flag chjcy dh ,d vueksy xks&lEink% vueksy ,d dh oekZ] vkèkkjA nqXèk xaxk(5)%17&19- dk djus lqjf{kr dks tkrh misf{kr ,d % egrk (2016) vgykor] ,oa nslh i'kqèku nslh ,oa fgekfy;u ;kdA i'kqèkuçdk'k8%39&41- dqekjh ,oa euh"kh eqds'k euh"kh ,oa dqekjh 6&10 44&47- vafdrk 'kekZ] vafdrk ,u ds v:i fxjh] v:i js[kk 'kekZ] foosd 'kekZ ,oa ,u ds ,u ,oa 'kekZ foosd 'kekZ] js[kk reu fæ; s xks d nè dh nwèk ds xèkksa esa ifjæ'; orZeku oekZ ] ] xkSj[key] & ,oa ,s ds pØorhZ cæh% u"h eqds'k] euh"kh e Hkxr] ,e qik f'kogjs] iq"ijkt fot; ds Hkkjrh] ds fot; (2017) usgk] lalkèku( çHkkr dqekj]nhid xxksbZ ,oa xxksbZ dqekj]nhid çHkkr mÙkjk[k.M fdyuh laØe.k ls nqèkk: ls laØe.k fdyuh jkds'k dqekj iqaMhj] dqekj jkds'k h s lg uhydkar flag] ds ih (2017) sk 'kekZ] js[kk n[h xk;% ynk[kh kfd lks<+h] eksfudk lesfdr Ïf"k% lesfdr i'kqèku çdk'k i'kqèku i'kqèku çdk'k i'kqèku kkSM h yEcs dh ukxkySaM s s qr vuqJh xqIrk ds ,s (2017) (2016) Xykscy okfeZax Xykscy o' dqekjh] ços'k 'ksys'k dqekj 'ksys'k dh hk vjksM+k jhuk ,d Hkkjrh; lksfudk çcUèku i'kqèku ynk[k nqèkk: lrr ços'k çhrh (8)% (8)% oekZ ubZ

Books 3. 2. 1. 12. RESEARCH PROJECTS &PUBLICATIONS 83537-36-5). by Director NBAGR, Karnal (ISBN: 978-93- Arjava Sharma (Pub. #106/2017)Published edited by NKVerma, and Aggarwal RAK ResourcesAnimal Genetic of India –Goats Sharma (ISBN: 978-93-83537-38-9). Ahlawat and Indrajit Ganguly and Arjava Perspectives edited by KN, Sonika Raja under Climate Change and Socio-economic Management of Resources Animal Genetic 83537-37-2). Jayakumar S and SK Niranjan (ISBN 978-93- and Poultry breeds edited by Arjava Sharma, situ In 96&98- (8)% çdk'k i'kqèku vkgkjA ikSf"Vd ,d fy, ds i'kqvksa esa vuko`fLV & lkbyst (2017) Hkxr ts Mh ,oa nk¡xh ,l ih iqaMhj] ds vkj nslkbZ] th ch pkSgku] Çlg gjsUnj cqjVs] th vkj dqekj] 'kkyw (8)% 56&58- (2017)- Hkkjr esa xkSèku dk egRoA i'kqèku çdk'k dqynhi yky tokgj ,oa lkgw ,l th ;kno] ch ,l nk¡xh] ,l ih iqaMhj] ds vkj nslkbZ] th ch NBAGR conservation of Indigenous Livestock 5. 4. 2. 1. ChaptersBook (ISBN: 978-93-83537-40-2). Published by Director NBAGR, Karnal N and Arjava Sharma (Pub. #111/2018). edited by AKMishra, Vikas Vohra, K Raja ResourcesAnimal Genetic of India: Sheep 39-6). NBAGR, Karnal (ISBN 978-93-83537- (Pub. #109/2018).Published by Director edited by Vikas Vohra and Arjava Sharma ResourceAnimal Genetic of India – Cattle 93-83537-37-2). Jayakumar S and SK Niranjan (ISBN 978- and Poultry breeds”. Ed. Arjava Sharma, situ “ entitled in book breed conservationthe in RoleMishra(2017) AK of Goshala and NGOs 83537-38-9). Ganguly and Arjava Sharma (ISBN 978-93- Ahlawat, KN,Sonika Ed. Raja Indrajit Change and Socio-economic perspectives”. of Resources Animal Genetic under Climate entitled book inthe scenario “Management and production system under climate change AK Mishra (2017)Indigenous breeds poultry conservation of Indigenous Livestock jk-i-vk-la-C;wjks In In

57 ANNUAL REPORT 2017-18 PUBLICATIONS 58 ANNUAL REPORT 2017-18 PUBLICATIONS 4. 3. 7. 6. 5. jk-i-vk-la-C;wjks of threatened breeds situ and in Ahlawat (2017)Strategies for conservation MS Tantia, Sharma Rekha and Sonika November 9-10,2017. ICAR- NBAGR, Makhdoom, Mathura from products value addition and safety their at National on Scope upscaling production to National Seminar on Small Ruminants: for Conservation in compendium of Priortization of Ruminant small resources Arjava Sharma and NK Verma (2017) 978-93-83537-38-9). Indrajit Ganguly and Arjava Sharma (ISBN perspectives”. Ahlawat, KN,Sonika Ed. Raja under Climate Change and Socio-economic “Management of Resources Animal Genetic and Implications” entitled book inthe ResourcesGenetic of India: Approaches NK Verma (2017)Characterization of Animal 93-83537-37-2). Jayakumar S and SK Niranjan (ISBN 978- and Poultry breeds”. Ed. Arjava Sharma, conservation of Indigenous Livestock situ populations entitled book inthe “In characterization of livestock and poultry forAhlawat methodology (2017) Survey MS Tantia, Sharma Rekha and Sonika 978-93-83537-38-9). Indrajit Ganguly and Arjava Sharma (ISBN perspectives”. Ahlawat, KN,Sonika Ed. Raja under Climate Change and Socio-economic “Management of Resources Animal Genetic of indigenous entitled AnGR book inthe Ahlawat (2017)Strategies for conservation MS Tantia, Sharma Rekha and Sonika Niranjan (ISBN 978-93-83537-37-2). Ed. Arjava Sharma, Jayakumar Sand SK Indigenous Livestock and Poultry breeds”. situ entitled book the “In RESEARCH PROJECTS &PUBLICATIONS NBAGR conservation of models in

13. 12. 11. 10. 9. 8. and Poultry breeds”. Ed. Arjava Sharma, “ Discrimanant analysis entitled book inthe on morphometric traits using Canonical RK Pundir (2017)Breed differentiation based 93-83537-37-2). Jayakumar S and SK Niranjan (ISBN 978- and Poultry breeds”. Ed. Arjava Sharma, situ and breeds at watch entitled book inthe “In RK Pundir (2017)Indigenous cattle diversity 978-93-83537-37-2). Sharma, Jayakumar Sand SK Niranjan (ISBN Livestock and Poultry breeds”. Ed. Arjava situ entitled “In of Animal genetic Resources book in the PK Singh and Indubala status (2017).Global Arjava Sharma (ISBN 978-93-83537-38-9). Ahlawat,KN, Sonika Indrajit Ganguly and and Socio-economic perspectives”. Ed. Raja ResourcesGenetic under Climate Change entitled book in the “Management of Animal for improvement and conservation of AnGR PK Singh (2017)State-wise breeding policy 93-83537-37-2). Jayakumar S and SK Niranjan (ISBN 978- and Poultry breeds”. Ed. Arjava Sharma, situ Indian Livestock entitled book inthe “In early warning and response system for PK Singh (2017) National watch list and Niranjan (ISBN 978-93-83537-37-2). Ed. Arjava Sharma, Jayakumar Sand SK Indigenous Livestock and Poultry breeds”. situ entitledbook “In and impending risk to its diversity inthe NK Verma (2017)Indigenous goat breeds In situ In conservation of Indigenous Livestock conservation of Indigenous Livestock conservation of Indigenous Livestock conservation of Indigenous conservation of 18. 17. 16. 15. 14. RESEARCH PROJECTS &PUBLICATIONS prospect for conservation book in the of indigenous livestock produce: Afuture and MS Tantia (2017) Characterization Sharma,Rekha Ahlawat, Sonika Arora Reena Niranjan (ISBN 978-93-83537-37-2). Ed. Arjava Sharma, Jayakumar Sand SK Indigenous Livestock and Poultry breeds”. situ entitled book in the “In Somatic approach cell for cryopreservation Sharma, Arora Reena and MSTantia (2017) Sharma,Rekha Ahlawat, Sonika Himani Sharma (ISBN 978-93-83537-38-9). Ahlawat,Sonika Indrajit Ganguly and Arjava Socio-economic perspectives”. Ed.KN, Raja Resources under Climate Change and entitled “Management of Animal Genetic of Resources Animal Genetic book inthe Markers for Diversity Genetic Analyses and Sharma Rekha (2017)Molecular Arora,Reena Mandeep Kaur, Ahlawat Sonika 93-83537-37-2). Jayakumar S and SK Niranjan (ISBN 978- and Poultry breeds”. Ed. Arjava Sharma, situ in farm animals entitled book in the “In approaches for genetic diversity analysis and Sharma Rekha (2017)Molecular Arora,Reena Mandeep Kaur, Ahlawat Sonika 83537-38-9). Ganguly and Arjava Sharma (ISBN 978-93- Ahlawat, KN,Sonika Ed. Raja Indrajit Change and Socio-economic perspectives”. of Resources Animal Genetic under Climate resources entitled book inthe “Management conservation of indigenous cattle genetic RK Pundir (2017)Role of gaushalas in 93-83537-37-2). Jayakumar S and SK Niranjan (ISBN 978- NBAGR conservation of Indigenous Livestock conservation of 22. 21. 20. 19. Springer. Ed. Joao Simoes and Carlos Gutierrez, Volume I(Welfare, Health and Breeding)”. goat production inadverse environments: Indian goats entitled book inthe “Sustainable diversity and population of structuring Nuclear and mitochondrial marker based AhlawatSonika and Sharma Rekha (2018) 978-93-83537-38-9). Indrajit Ganguly and Arjava Sharma (ISBN perspectives”. Ahlawat, KN,Sonika Ed. Raja under Climate Change and Socio-economic “Management of Resources Animal Genetic climatic conditions entitled book inthe livestock adapted species to different agro- evolution of immune response genes in and SK Niranjan (2017)Diversity and RS Kataria, SK Mishra, Namita Kumari 93-83537-37-2). Jayakumar S and SK Niranjan (ISBN 978- and Poultry breeds”. Ed. Arjava Sharma, conservation of Indigenous Livestock situ livestock entitled book inthe “In markersfunctional for improvement of (2017) Identification and genotyping of RS Kataria, SK Mishra and SK Niranjan 978-93-83537-38-9). Indrajit Ganguly and Arjava Sharma (ISBN perspectives”. Ahlawat, KN,Sonika Ed. Raja under Climate Change and Socio-economic “Management of Resources Animal Genetic endangered entitled book inthe species An alternate for method conservation of and MSTantia Banking: (2017)Somatic Cell Sharma,Rekha Ahlawat, Sonika Arora Reena 978-93-83537-37-2). Sharma, Jayakumar Sand SK Niranjan (ISBN Livestock and Poultry breeds”. Ed. Arjava situ entitled “In conservation of Indigenous jk-i-vk-la-C;wjks

59 ANNUAL REPORT 2017-18 PUBLICATIONS 60 ANNUAL REPORT 2017-18 AWARDS 24. 23. 2. 1. Monographs/ /Compendium Bulletin jk-i-vk-la-C;wjks increasing bovine productivity (2017) Biotechnological interventions for Arora,Reena RS Kataria and MS Tantia Ahlawat,Sonika SK Niranjan, Sharma, Rekha Arjava Sharma (ISBN 978-93-83537-38-9). Ahlawat,KN, Sonika Indrajit Ganguly and and Socio-economic perspectives”. Ed. Raja ResourcesGenetic under Climate Change entitled book the “Management of Animal Techniques for characterization of AnGR in Arora and MSTantia (2017)Molecular AhlawatSonika , Rabgais, Shakil Ahmad.Rabgais, Shakil Bhuvnesh Kumar, M.Iqbal, Stanzin Gogoi, SarangiKalia, Deepak Prabhu, Bharti, Arup Giri, Prabhat Kumar, Sahil Kumar Swami, Arjava Sharma, Vijay K Ankita Sharma, Parvesh Kumari, Shelesh K Niranjan, RKPundir, Preeti Verma, Mukesh, Monika RSKataria, Sodhi, Saket to High Altitude Manishi of Leh-Ladakh: Unique Resource Animal Genetic Adapted Technical Cattle: on bulletin A “Ladakhi Sharma (ISBN 978-93-83537-38-9). Ahlawat,Sonika Indrajit Ganguly and Arjava Socio-economic perspectives”. Ed.KN, Raja Resources under Climate Change and entitled “Management of Animal Genetic bdcky] ,ljkcxSl]'kdhyvgen lkfgy dkfy;k]nhidxksxksbZ] Hkqous'k dqekj],e 'kekZ ] fot; ds Hkkjrh] v:i fxjh] çHkkr dq ekj] vktZo Lokeh] ekj dq 'ksys'k ekjh] dq ijos'k 'kekZ] vkj ,l dVkfj;k] vkj ds iqaMhj] çhrh fujatu] oekZ] vafdrk ds lkdsr lks

61 ANNUAL REPORT 2017-18 AWARDS 62 ANNUAL REPORT 2017-18 AWARDS 9. 8. jk-i-vk-la-C;wjks Center, Nueva Ecija, Philippines. 26-27, 2017 atOctober Philippines Carabao EnterpriseCarabao Based Development, system” International inthe Conference on recording buffalo and dairy survey field “Application of information in technology presented an invited lecture on topic the Dr. Avnish Kumar, Principal Scientist 2017) at ICAR-NBAGR, Karnal awareness week (30 Arora and Sharma Rekha during Vigilance by Himani Sharma, Mandeep Kaur, Reena entitled “My vision-corruption India” free poster award best Second for research the RESEARCH PROJECTS &PUBLICATIONS th October – 4 October NBAGR th November,   11. 10. Mukesh Mat 36 by Sharma A,Kumar M,Jain M,Sodhi Pand A potential candidate for resistance” disease variants and haplotypes inlactoferrin gene: Delineating Indian native cattleallelic specific poster awardBest for research the entitled” June 2017 5th, field of Sciences Agriculture onAllied & achievement and accomplishment inthe (SVWS)Society on account of overall Scientist Award” by Samgra Vikas Welfare Dr. Monika received “Eminent Sodhi Ireland from 16-21st July 2017. of held Animal at Genetics Society Dublin in th conference of International Capacity Building • • • • NBAGR Mera GaonMera Gaurav Exhibitions Trainings Attended Trainings Organized jk-i-vk-la-C;wjks

63 ANNUAL REPORT 2017-18 64 ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks NBAGR 3. 2. 1. technical and supporting staff of ICAR institutes. at NBAGR for of upscaling skill the administrative, Following training programmes were conducted Trainings Organized Capacity B o`f¼ ,oa O;fäRo fodkl gsrq çf'k{k.k dk;ZØe] çf'k{k.k gsrq fodkl O;fäRo ,oa o`f¼ of ICAR entitled A Training program for Supporting Skilled Staff 28-31, 2017at ICAR-NBAGR. Husbandry,Animal Haryana during August organized for of officials Department of Training on ‘PCRdetection of beef’ based Karnal. during June 27-29,2017at ICAR-NBAGR, conducted under ICAR-HRD program and administrative professionals” was professional competence of ICAR technical A Training program on “Enhancement of 29-31, 2017at ICAR-NBAGR, Karnal. conducted under ICAR-HRD during August NBAGR

“ dq'ky lgk;d LVkQ dh ;ksX;rk uilding ” was ” was February, 2018. 5. 4. Sciences, BikanerSciences, (Rajasthan) from 8 Rajasthan University of Veterinary &Animal ofwith College Veterinary &Animal Science, Biodiversity (SOCDAB) incollaboration forby of Conservation Society Domestic Animal for enhancing Farmers’ the income” was organized Management of Livestock and Poultry Diversity National Symposium on “Sustainable National Symposium Resources under Climate Change and ofConservation Indigenous Animal Genetic Model Training for on ‘Strategies Course and above of SAUs ICAR, and SVUs. November 3,2017for Assistant Professors and Poultry Breeds” from 25to October ofConservation Indigenous Livestock ICAR situ sponsored Short on Course “In Board. Board. SAHD, SLDB/agencies and State Biodiversity from officers November of 23-30, 2017 for Socio-economic Perspectives’ was organized jk-i-vk-la-C;wjks th -10 th

65 ANNUAL REPORT 2017-18 Trainings organized 66 ANNUAL REPORT 2017-18 Trainings Attended 3. 2. 1. Trainings Attended jk-i-vk-la-C;wjks fodkl gsrq çf'k{k.k dk;ZØe “ Sh.Satbir attendedtraining programmeon and Deepak Sagar,Sh. Sh.Ram Lal, Krishan Sh. including staff supporting Skilled All ICAR-NDRI. analysis” from August 21-26,2017at programme on “Basic training on chemical Sh. Kumar Ramesh (TO) attended training 27-29, 2017 at ICAR-NBAGR. and administrative professionals” from June professional competence of ICAR technical training programme on “Enhancement of (STO) and Sh. Naresh Kumar attended (LDC) Anita Chanda (PA), Sh. Harvinder Singh S. Dangi (CTO) ,Sh. S.K.Jain (ACTO), Smt. Sh. Karambir Malik (PSto Director), Dr. P. 2017 at ICAR-NBAGR,Karnal. dq'ky lgk;d LVkQ dh ;ksX;rk o`f¼ ,oa O;fäRo NBAGR ” from August 29-31, 7. 6. 5. 4. ICAR- Karnal. IIWBR, 14-16,2017at December laboratory” from &biotechnologyin biochemistry “ on Division underwent atraining programme KumarSh. Rakesh of Animal Biotechnology Technical officers, Sh. Naresh Kumar and 2017 organized by ILRI at ICAR-CIRB, Hisar. Genomic Prediction” from November 20-24, on “Analysis of Animal Breeding Data and Dr. RKPundir attended training programme September 22, 2017 at ICAR-NDRI, Karnal. PFMSthe Training by ICICI BANK on Pawan Kumar Gupta (AF&AO) attended &AO), Sh. Harish (AO), Arya Sh. Sunil Kumar (F September 19-23,2017at CIAE Bhopal. road safety &behavioural skills” from programme on “Automobile maintenance, Sh. (Technician) Balwinder attended training Capacity B Proficiency enhancement for working Sh. SinghSh. (AAO) Balkar Sh. and uilding 4. 3. 2. 1. Exhibitions Capacity B 6. 5. AnGR exhibition was organised at 10 Meladuring Dairy on November 23-25, 2017. of was organized country the at NDRI, Karnal An exhibition on Resource Animal Genetic Haryana from 27-29,2017. October organized by Animal Husbandry Department Jajhar on occasion the of Livestock Show diversity and its utility at Police ground, Line An exhibition explaining farm animal the Dayoccasion of on 10,2017. Seed October diversity and its utility at ICAR-IIWBR on the An exhibition explaining farm animal the 2017. Jhalan, Patiala, Punjab from 1-5, December Livestock Championship, 2017held at village mobilized 16 progressive farmers from Barota during March 16-18,2018.The ICAR-NBAGR Unnati Mela at IARI Mela ground, New Delhi An exhibition was organised during Krishi the Centre) Karnal held on February 17,2018. Mela at Sugarcane Breeding Institute (Regional An exhibition was organised during Kisan the NBAGR uilding th

  Mera GaonGaurav were sensitized about also issues. various social various of Animal aspects Husbandry. People ofvillages Karnal and delivered lectures on Sambhli, Bras, Sirsi, Chiaro and Badshahpur Narukheri, Pingli, Dadupur, Shahjanpur, Gaurav programme. Bureau team visited were strengthened through Mera Gaon Mera Linkages with farmers and livestock keepers 7. (Haryana) held from March 24-26,2018. summit Kisan 2018cum Mela at Rohtak was organised during Haryana’s 3 An exhibition of farm animal genetic resources address and visit of various stalls. ofvillage Karnal to attend Prime Minister’s jk-i-vk-la-C;wjks rd Agri-

67 ANNUAL REPORT 2017-18 Exhibitions 68 ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks NBAGR Other Activities Distinguished Visitors • Celebrations • Important Meetings • Library • NBAGR jk-i-vk-la-C;wjks

69 ANNUAL REPORT 2017-18 70 ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks NBAGR held on April 28-29,2017 wherein final reports Institute Research Committee (IRC) meeting was Institute Research Committee RAC at occasion. this of Cattle and buffalo breeds were thereleased by Technology, The Banda. calendars breed revised Chancellor, Banda University of Agriculture and chairmanshipthe of Dr. SL Goswami, Vice (RAC) was held on April 3, 2017 under A meeting of Research Advisory Committee Committee Research Advisory Meetings Important scientific readers. journals have for subscribed benefit the been of Seventeen Indian Journals and Six foreign procured during library in the period. the and journals worth Rs.7,98,306/-wereBooks purchase of etc.books/journals scientific management of issues library the to pertaining Committee (LAC) is guiding force inthe stafftechnical the of bureau. Library Advisory important scientists the role and in serving The NBAGR playing has library been an Library OTHER ACTIVITIES No. of Newspapers subscribed subscribed No. of Indian/ Foreign Journals No. of added books Total collection NBAGR 4196 07 23 05 livestock were and poultry approved in meeting. New RegistrationDelhi. of nine breeds new of held on August 4,2017at Krishi ICAR, Bhawan, A meeting of Breed registration Committee was RegistrationBreed Committee Meeting AHD, Jammu &Kashmir. Institute of High Altitude Research andof Officers Scientists of ICAR-NBAGR and Defense DRDO- Scientists from ICAR Head Quarter, Director and (AP&B)andby ADG Councilors of LAHDC, and double hump camel. Meeting was attended non-descriptlocal cattle, Zanskari horse, donkey animal genetic resources yak, Ladakh especially issues for characterization and evaluation of to various discuss related Leh-Ladakh (LAHDC), AutonomousLadakh Hill Development Council Region was held onof May Ladakh 10,2017at An Interactive on meet “Animal Resources Genetic Interactive Meet project pending the new discuss proposals. IRC meeting was held on September 19,2017to proposals were and approved. discussed Another of completed the projects and project new jk-i-vk-la-C;wjks

71 ANNUAL REPORT 2017-18 IMPORTANT MEETINGS 72 ANNUAL REPORT 2017-18 Celebrations different indigenous livestock and poultry. Award- 2015for and conserving maintaining states were felicitated with Breed Saviour Twenty farmers / breeders representing various as chief function the guest.Fisheries) graced J.K. Jena, Deputy Director (AS & General Award ceremony from May 21- 22 2017. Dr. International Biodiversity and Breed Saviour with SEVA, Madurai, Tamil Nadu organized ICAR-NBAGR, Karnal incollaboration International Biodiversity Day Celebrations The 70 DayIndependence positions, respectively. Prerna Kapoor first,second secured thirdand Group, Ankita Sharma, Himani Sharma and received awards. the Among research scholars andSchool Adarsh Public (Third) School Tagore Niketan Bal The (Second), Millennium group, School the Public (First), Delhi School Research Scholars of ICAR-NBAGR, Karnal. In organizedwas also for students school and subject “Biodiversity and its conservation” occasion,On this aposter competition on the Director hoisted tricolor the and addressed attended programme. the Dr. Arjava Sharma, staff, the SRFs,All RAs and families their on August 15th Bureau inthe campus. jk-i-vk-la-C;wjks th Independence Day was celebrated NBAGR participated actively. conductedalso and in staff which wards their gathering.the Various cultural were activities different livestock was acknowledged species keepers inconservation of diverse breeds from were awarded. The contribution of livestock Sh. OmPrakash and Sh. Jita respectively Ram, Scientist. In technical and administrative category conferred upon Dr. Sharma, Rekha Principal genetic resourcespoultry of was country the conservation and utilization of livestock and of identification,evaluation, characterization, Nair Award for scientific contributionthe field in honour on occasion. The this prestigious Dr. P.G. Canara Karnal) Bank, (DGM, were guests the of AP&B, ICAR) and Sh. Umesh Kumar Sharma was chief the guest and Dr. Gandhi (ADG, R.S. Pathak, Vice-Chancellor, UPPDUPVV, Mathura Day on 21st September, 2017.Dr. K.M.L. Resources, Karnal celebrated its Foundation 34th ICAR-National Bureau of Animal Genetic Foundation Day OTHER ACTIVITIES excitement. and cultural program with enthusiasm and of tricolour. Children the participated in quiz National Anthem was played after unfurling presided over by Director, Dr. Arjava Sharma. andfervour enthusiasm. The was function the chiefthe guest. inspiring Foundation Day lecture delivered by K.M.L. Pathak. The programmewith ended an released on occasion this by chief the guest, Dr. Mouli sheep and Yelga sheep respectively were three breed monographs on Nagaland goats, The eighthedition of “Pashudhan Prakash” and issue of “Pashudhan Prakash” were awarded. also Dinesh Gulati and his team. guidancethe of awell-known Yoga teacher, Sh. with families their attended Yoga the camp under employees, RA/SRF/JRF, students/scholars along Bureau premises June on 21,2017.A Yoga Day and organized a Yoga camp in the ICAR-NBAGR, Karnal celebrated International International Yoga Day 69 Staff and families of Bureau the celebrated Day Republic awarded.best The articles publishedthe 7 in organized during “Hindi Pakhwada” were also Awards. Thewinners of various competitions bybestowing with them Breed Conservation OTHER ACTIVITIES th Republic Day on 26 Day on Republic NBAGR th January, 2018 with January, 2018 ll the the ll th

celebrations infuture as well. schools expressed desire their of part to such be The teachers and students participatingthe of competition that was organized on occasion. this schools enthusiastically participated inaquiz national development”. Students from the all on “Livestocktalk biodiversity and its role in Sharma, Director of Bureau the delivered a and institute’s the research work. Dr Arjava animalthe genetic resources of country the students (120) of fiveschools were briefed about open day for students. the participating The exist sector. agricultural inthe It was kept an studentsthe about career the opportunities that programmethis was to generate awareness among NBAGR on 3,2017. Theof December purpose Agricultural Education Day was celebrated at Day Education Agricultural NBAGR Foundation Day. actively. The winners were given prizes on including and ladies wards their participated from January 23-24,2018where most of staff the organizedalso an annual formeet sports its staff As of apart Republic Day celebration, NBAGR Carrom Board, Chess and athletic events. & shooting), Kabaddi, Badminton, Table Tennis, participated Volley inBasket Ball, (smashing Ball 30to NovemberOctober 2,2017.The contingent heldsports at ICAR-IISR, Lucknow from NBAGR participated ICAR in the (North) Zonal A contingent of members 22staff of ICAR- EventsSports jk-i-vk-la-C;wjks

73 ANNUAL REPORT 2017-18 Celebrations 74 ANNUAL REPORT 2017-18 Distinguished Visitors 2. 1. Distinguished Visitors jk-i-vk-la-C;wjks on International Biodiversity Day. Breed Saviour Award ceremony organized Karnal on 22.05.2017 for participation in (AP&B), ICAR visited ICAR-NBAGR, ICAR;Sciences), and Dr. R.S.Gandhi,ADG Dr. JKJena, (Animal and DDG Fishery others visited on 06.05.2017. Dr. Singh, Director, R.K. ICAR-IVRI and ofCouncil Agricultural Research along with Indian(Fisheries &Animal Science), Director Dr. General, J. K.Jena, DDG Dr. T. Mohapatra, Secretary, DARE and NBAGR  7. 6. 5. 4. 3. Institute visited on 03.03.2018. Math from Management Development Dr. Shiv Tripathi and Prof. Bhusnur N.R. ICAR visited on 13.02.2018. Sh. R P Singh, Member, Governing Body, visited on 24.10.2017. A Doordarshan Team Delhi from New 21.09.2017. Director (AP&B),ICAR General visited on Mathura and Dr. R.S.Gandhi, Assistant ICAR and Vice-Chancellor, DUVASU, Dr. K.M.L.Pathak, Former (AS), DDG visited on 10.07.2017. in EmbassySpecialist of USA, New the Delhi Dr. Santosh Kumar Singh, Agricultural OTHER ACTIVITIES Personnel Superannuation • Joinings/Transfers • Promotions • Personalia • NBAGR jk-i-vk-la-C;wjks

75 ANNUAL REPORT 2017-18 76 ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks NBAGR Technical *Not physically present inNBAGR. Scientific Personalia PERSONNEL 3. 2. 1. No. Sl. 29. 28. 27. 26. 25. 24. 23. 22. 21. 20. 19. 18 17. 16 15. 14. 13. 12. 11. 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. S.No NBAGR Dr. Ahlawat Sonika Dr. Jayakumar S. Dr. K.N.Raja Dr. Karan Veer Singh Dr. P. Kathiravan* Dr. Sanjeev Singh Dr. Indrajit Ganguly Dr. Saket Kr. Niranjan Dr. Vikas Vohra Dr. Sharma Rekha Dr. Behl Rahul Dr. Avnish Kumar Dr. Manishi Mukesh Dr. Arora Reena Dr. Dinesh Kumar Dr. Jyostna Behl Dr. Satpal Dixit Dr. Monika Sodhi Dr. Kumar Anil Mishra Dr. Kataria R.S. Dr. Pundir R.K. Dr. P.K. Singh Dr. Aggarwal R.A.K. Dr. N.K.Verma Dr. P.K. Vij Dr. M.S.Tantia Dr. Anand Jain Dr. Vijh R.K. Dr. Arjava Sharma Name Dr. P.S. Panwar Sh. S.K.Jain Dr. P. S.Dangi Name Scientist (SS) Scientist (SS) Scientist (SS) ScientistSenior ScientistSenior ScientistSenior ScientistSenior Scientist*Senior Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Director Designation Asstt. Technical Chief Officer Asstt. Technical Chief Officer TechnicalChief Officer Designation jk-i-vk-la-C;wjks

77 ANNUAL REPORT 2017-18 Personalia 78 ANNUAL REPORT 2017-18 Personalia Skilled Supporting Skilled Administrative 4 3. 2. 1. 17 16. 15. 14. 13. 12. 11. 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. Sl. No. Sl. No. Sl. 16. 15. 14. 13. 12. 11. 10. 9. 8. 7. 6. 5. 4. jk-i-vk-la-C;wjks Sh. Singh Balwinder Sh. Chand Ramesh Sh. OmPrakash Sh. Ashok Kumar Sh. Subhash Chander KumarSh. Rakesh Sh. Kumar Ramesh Sh. Naresh Kumar Smt. Pravesh Kumari Sh. Jamer Singh Sh. Sat Pal Sh. Harvinder Singh Sh. Sanjeev Mathur Sh. Satbir Sh. Deepak SagarSh. Ram Sh. Krishan Lal Sh. Babu Ram Sh. Naresh Kumar Smt. Neerja Kaul Sh. Shiv Chander Sh. Satish Kumar Sh. Yoginder (Presently on deputation) Sh. Jita Ram Smt. Shashi Bala Smt. Indu Bala Smt. Amita Kumari Smt. Anita Chanda Sh. Behl Ramesh Sh. Pawan Kr. Gupta SinghSh. Balkar Sh. Karambir Sh. Sunil Kumar Sh.Raj Kumar Name Name NBAGR SSS SSS SSS SSS LDC LDC LDC LDC UDC Assistant Assistant Assistant Steno Gr.III PA PA Assistant AF&AO AAO PS to Director F&AO A.O. Designation Designation Technical Assistant (Driver) TechnicalSenior Assistant TechnicalSenior Assistant Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer TechnicalSenior Officer Asstt. Technical Chief Officer PERSONNEL 6. 5. 4. 3. 2. 1. Promotions PERSONNEL w.e.f. 07.02.2013under CAS. to of next grade the higher Scientist Senior Dr. Karan Veer Singh promoted has been under Career Advancement Scheme(CAS). ofpost Principal Scientist on 02.04.2017 Dr. Vikas Vohra promoted has been to the Technical Officer w.e.f. 02.01.2017. i.e. nextthe grade higher Assistant Chief Sh. Sanjeev Mathur promoted has been to (Driver) w.e.f. 18.01.2016. of nextthe grade higher Technical Assistant Sh. Singh promoted Balwinder has been to 24.02.2016. ofnext grade higher Technical Officer w.e.f. Sh. Ashok Kumar promoted has been to the Technical Officer w.e.f. 28.02.2013. i.e. nextthe grade higher Assistant Chief Dr. P.S. Panwar promoted has been to NBAGR   4. 3. 2. 1. Joinings/Transfers 1. Superannuation (FN) as Administrative Officer. Sh. Raj Kumar joined NBAGR on 26.12.2017 on 27.12.2017. NDRI Karnal and relieved from NBAGR ofpost Administrative Senior at Officer promoted has been Arya Sh. H.R. to the deputation basis on 20.12.2017. commission of India, New on Delhi ofpost Assistant officer Section Election at Sh. Yoginder appointed has been to the 01.04.2017. on Administrative post the w.e.f. Officer Regional Station Karnal joined NBAGR the Administrative Arya, Sh. H.R. Officer, IARI superannuated on 31.03.2018. Sh. Ashok Kumar, Technical Officer jk-i-vk-la-C;wjks

79 ANNUAL REPORT 2017-18 Promotions 80 ANNUAL REPORT 2017-18 jk-i-vk-la-C;wjks NBAGR fgUnh [k.M • • • NBAGR jktHkk"kk izdks"Bdhxfrfof/k;k¡ dk;Zdkjh lkjka'k funs'kd dhdyels--- jk-i-vk-la-C;wjks

81 ANNUAL REPORT 2017-18 82 okf"kZd izfrosnu 2017-18 jk-i-vk-la-C;wjks jk- i-vk-la-C;wjks ,oa varjkZ"Vªh; Lrj ij egRo c<+sxkA ekaM;k HksaM+ dh HksaM+ ekaM;k c<+sxkA egRo ij Lrj varjkZ"Vªh; ,oa jk"Vªh; dk uLyksa dh HkSalksa ,oa xk;ksa ns'kh esa Hkfo"; vkuqoaf'kd :ijs[kk dk Hkh vè;;u fd;k x;k gS] blls nkSjku Lons'kh eosf'k;ksa esa ,1 ,2 vyhYl vkSj mudh bl gSaA x;h dh 'kq: Hkh ifj;kstuk,a vU; gsrq vè;;u ds lewgksa @ uLyksa Kkr de dh çtkfr;ksa ikyrw vU; ,oa cdjh HksaM+] HkSal] xk;] vykok blds x;k- fd;k vè;;u ls ekè;e ds losZ{k.k ls :i vkuqoaf'kd ,oa çk:fid dk lewg ;kd ds yík[k vkSj cdjh uUnhnqxkZ ,oa chnjh dh dukZVd HksaM+] nqEck HksaM+] fprjaxh dh jktLFkku ,oa iatkc HkSal] ekunk dh mM+hlk i'kqvksa] xksoa'kh voÆ.kr ds rfeyukMq ,oa dsjy nkSjku ds o"kZ ls çLrqrfd;kx;kgSA dh xkSjoe;h lexz miyfCèk;ksa dks bl fjiksVZ ds ekè;e 2017&18 o"kZ dh laLFkku gSA x;k fd;k iathdj.k 1 ;kd] 1 cÙk[k+ ] 1 xhtÛ ,oa 1 [kPpj dh uLyksa dk 'kwdj] 7 eqxhZ] 18 Å¡V] 9 Vêw] ,oa ?kksM+s 7 HksaM+] 42 cdjh] 28 HkSal] 13 xk;] 41 }kjk laLFkku nkSjku ds ;k=k xkSjoe;h bl dh vcrd ls LFkkiuk viuh gS- ç;kljr gsrq mi;ksx lrr muds ,oa mi;ksx laj{k.k] igpku] egRo] ds lalkèkuksa vkuqoaf'kd i'kq ns'kh tksfd gS laLFkku ,dek= dk ns'k ;g gSA ç;Ru'khy gsrq ykus tkx:drk esa i'kqikydksa esa ckjs ds egRo ds lalkèkuksa vkuqoaf'kd i'kq Hkkjrh; ls :i vfojy laLFkku ;g ;g ls 1984 flrEcj 21 fnol LFkkiuk vius eglwl djjgkgw¡A xkSjokfUor vkidks vius gq, djrs lk>k vkils dks gS% ys[kk&tks[kk dk miyfCèk;ksa dh 2017&18 o"kZ fd tks çfrosnu okf"kZd dks] miyfCèk;ksa çkIr ,oa dk;ks± x, foLrkj] f'k{kk ,oa rduhdh foLrkj vkfn ds {ks=ksa çf'k{k.k] esa fd;s vuqlaèkku] }kjk ds djuky C;wjks] lalkèku vkuqoaf'kd i'kq jk"Vªh; & HkkÏvuqi laLFkku çfrf"Br eSa funs'kd ds :i esa] jk"Vªh; ,oa varjkZ"Vªh; [;kfr çkIr funs'kd dhdyels--- fgUnh [k.M jk- i-vk-la-C;wjks mUgsa vkxsc<+uslsjksdktkldsA çtud ujksa ds vkuqoaf'kd nks"kksa dk irk yxkuk gS ftlls mís'; ftldk gS] jgk dj çnku Hkh lsok ijke'kZ gsrq ijh{k.k jksx fofHkUu vkSj LØhÇux lk;VkstsusfVd dh ujksa çtud jgs gks ç;ksx esa laLFkkvksa fofHkUu ds ns'kHkj vk;sxhA gekjk laLFkku Hkkjr ljdkj dh uhfr ds rgr] uLyksa esa o`f¼ gksxh ,oa voÆ.kr i'kqvksa dh la[;k esa deh iathÏr esa le; okys vkus ftlls gSA x;k fd;k gsrq 10 u, i'kq lewgksa dk lekos'k] muds y{kuhdj.k djus varxZr ds ifj;kstuk usVodZ lalkèku vkuqoaf'kd i'kq Mkst dklekos'kfd;kx;kgSA esa xksoa'kh uLyksa Mkaxh] jkBh] f[kYykj ,oa ukxksjh ds oh;Z cSad thu jk"Vªh; ds laLFkku gSA x;h cukbZ ykbZu lsy QkbZczksCykLV dh ÅaVksa dPNh ,oa ?kksM+s ef.kiqjh rgr ds gfj;k.kk uLydscPpsgSaA,Dl&lhVwlaj{k.kdk;ZØe 41 ,oa lkfgoky 5 ftuesa gS pqdk gks tUe dk cPpks 46 dqy esa ifj;kstuk bl ,oa gS ij çxfr dk;Z ds laj{k.k ds uLy gfj;k.kk ,oa lkfgoky ls ekè;e ds xks'kkykvksa rgr blds gS vfèkns'k egRoiw.kZ ,d dk C;wjks lalkèku vkuqoaf'kd i'kq jk"Vªh; laj{k.k uLy vè;;u Hkhfd;kx;kgSA ds fy, daMhMsV thu dh igpku ,oa muds fØ;kfofèk dk y{k.kksa lacaèkh etcwrh mudh ,oa lgu'khyrk esa Vêqvksa tULdkj jgs tk ikys ij Å¡pkbZ ,oa xq.koÙkk ek¡l mÙke jk-i-vk-la-C;wjks

83 okf"kZd izfrosnu 2017-18 funs'kd dh dye ls --- 84 okf"kZd izfrosnu 2017-18 funs'kd dh dye ls --- fo Ï" vqaku o fkk fHk@sj ,oa egkfuns'kd] Hkkjrh; Ïf"k vuqlaèkku ifj"kn~] ubZ fnYyh foHkkx@Ms;j) f'k{kk ,oa vuqlaèkku (Ïf"k lfpo egkik=k] Vh MkW o"kZ fiNys yhA #fp dkQh us yksxksa egRo ds ckjs esa tkx:drk QSykus gsrq fd;k x;k ftlesa ds i'kqvksa ns'kh ,oa crkus dks xfrfofèk;ksa dh laLFkku esa fgLlksa fofHkUu ds ns'k vk;kstu dk çn'kZfu;ksa ikap }kjk laLFkku fy;kA Hkkx ls :i lfØ; us deZpkfj;ksa ,oa vfèkdkfj;ksa oSKkfudksa] ds laLFkku esa vfn vfHk;ku LoPNrk jk"Vªh; ,oa foKku] t; fdlku t; xkSjo] esjk xkao esjk fd tSls dk;ZØeksa jk"Vªh; fofHkUu ds ns'k feyh gStgk¡ijbUgksausviusdk;ks±dksçLrqrfd;kA igpku Hkh ij epksa varjkZ"Vªh; ,oa jk"Vªh; fofHkUu dks dk;ks± x, fd;s }kjk muds ,oa fo'ks"kKrk dh oSKkfudksa nkSjku 5 çf'k{k.k dk;ZØe vk;ksftr fd;s x,A C;wjks ds fodkl ds fy, Hkh ;ksxnku dj jgk gS ftlds rgr bl {kerk esa yksxksa layXu esa dk;ZØeksa çlkj ,oa fpfdRlk çdkf'kr fd;s x,A laLFkku i'kq ikyu f'k{kk] vuqlaèkku] }kjk oSKkfudksa ds laLFkku Hkh vè;k; iqLrd 24 ,oa eksuksxzkQ 5 fdrkcsa] 5 vfrfjä blds gq,A çdkf'kr esa if=dvksa 'kksèk ,oa tuZy [;kfrçkIr varjkZ"Vªh; ,oa jk"Vªh; fofHkUu ys[k ys[k@yksdfç; rduhdh 12 ,oa i= 'kksèk 33 nkSjku bl gSA 'kkfey ifj;kstuk Qsyks us'kuy ,d vkSj iksf"kr foÙk cká 5 ftlesa gS tkjh gqbZ ,oa orZeku esa 26 vuqlaèkku ifj;kstukvksa ij dk;Z iw.kZ ifj;kstuk,a vuqlaèkku 7 dqy o"kZ fiNys x;hA dh çnku eatwjh dks ifj;kstukvksa ubZ ,oa x;h dh leh{kk dh dh ifj;kstukvksa dk;Zjr fjiksVZ] çxfr vafre dh ifj;kstukvksa gqbZ iw.kZ nkSjku ds o"kZ ftlesa gSa jgh gks vk;ksftr ij le; cSBdsa dh lfefr vuqlaèkku laLFkku jk-i-vk-la-C;wjks jk- i-vk-la-C;wjks   dk LokxrdjrsgSaA ges'kk gh lqèkkj ds fy, vkids lq>ko vkSj vkykspukvksa dh miyfCèk;ksa ds vkdkj esa vkids lEeq[k çLrqr gSA ge C;wjks nLrkost+ ;g ls ifjJe ftuds gw¡ djrk èkU;okn Hkh dks lnL;ksa ds eaMy lEiknd ds çfrosnu okf"kZd eSa] lkFk gh lkFk gw¡A nsrk cèkkbZ lkFk ds xoZ fy,] ds ;ksxnku muds esa çxfr dh laLFkku dks deZpkfj;ksa ,oa vfèkdkfj;ksa oSKkfudksa] lHkh ds laLFkku eSa gw¡A djrk lfefr ls çkIr lykg ,oa ekxZn'kZu gsrq eSa ÏrKrk Kkfir lykgdkj vuqlaèkku gw¡A vkHkkjh ls ân; gsrq ekxZn'kZu gsrq fodkl lexz ds laLFkku dk fnYyh ubZ ifj"kn~] vuqlaèkku Ïf"k Hkkjrh; oSKkfud] çèkku Hklhu] fouhr MkW ,oa çtuu) ,oa mRiknu (i'kq xkaèkh] egkfuns'kd lgk;d& ,l vkj MkW- foKku)] i'kq ,oa foKku (eRL; ifj"kn~ ubZ fnYyh ,oa MkW- ts ds tsuk] mi& egkfuns'kd vuqlaèkku Ïf"k Hkkjrh; egkfuns'kd] ,oa foHkkx@Ms;j) f'k{kk ,oa vuqlaèkku (Ïf"k lfpo egkik=k] Vh MkW- eSa] iqjLdkjß Hkh'k:fd;kgSA laj{k.k ÞuLy fy, ds leqnk;ksa ,oa laLFkkvksa O;fä;ksa] laLFkku us ns'kh iathÏr i'kq uLyksa ds laj{k.k esa layXu mRlkg ds lkFk euk;k x;kA orZeku o"kZ ls gh çFke ckj iw.kZ i[kokM+k fganh ,oa lIrkg lrdZrk fnol] fofoèkrk Lora=rk fnol] laLFkku LFkkiuk fnol] varjkZ"Vªh; tSo dk;ks± dh ljkguk dhA C;wjks ifjokj }kjk x.kra= fnol] ds C;wjks ,oa fd;k nkSjk dk laLFkku us foKku) i'kq ,oa foKku (eRL; egkfuns'kd mi& tsuk] ds ts MkW ,oa fgUnh [k.M (vktZo 'kekZ) funs'kd usVodZ (,,u,u) vkSj leFkZu osDVj e'khu (,loh,e) e'khu osDVj leFkZu vkSj (,,u,u) raf=dk usVodZ Ïf=e & vuqekuh vkSj (Mh,WQ+,)] fo'ys"k.k Hkkjr dh vkB Lons'kh HksM+ uLyksa dks foHksnd dk;kZRed xbZ A dh ,d= ls fgrèkkjdksa vU; vkSj okyksa j[kus i'kqèku @ fdlkuksa tkudkjh vkèkkfjr ç'ukoyh ij xfrfofèk;ksa dh i'kqikyu x;kA fd;k 'kq: lky bl vè;;u fy, ds ewY;kadu ds dk;ZØeksa vkSj uhfr;ksa i'kqèku fy, ds gfj;k.kk vkSj iatkc esa i'kq vkuqoaf'kd lalkèku ds çcaèku 169 rdigqapxbZgSA la[;k dqy dh uLyksa Lons'kh iathÏr rjg bl fd;kA ckj ;kd] cr[k vkSj gal dh Lons'kh uLyksa dks iathÏr igyh us laLFkku FkhA ls {ks= iwoksZÙkj ds ns'k uLysa ikap ?kksM+k] x;hA dh iathÏr fpdu vkSj cÙk[k gal] ;kd] lqvj] ,d çfr vkSj nks dh cdjh ,d] dh uLy& xksoa'k ubZ ukS dh dqôqV vkSj i'kqèku Lons'kh }kjk C;wjks lalkèku vkuqoaf'kd i'kq jk"Vªh; Hkk-Ï-vuq-i-& yk[k jktLods:iesaçkIrfd;sx,A dqy O;; gqvkA foÙkh; o"kZ 2017&18 esa #-35-66 dk yk[k #-76-6 ls çkfIr dqy dh yk[k #-77-0 rgr] ds ifj;kstuk usVodZ gqvkA O;; dqy dk yk[k rgr 9 #-1318-4 esa ds çkfIr dqy dh ykHk) yk[k #-1335-3 lsokfuo`fÙk vkSj isa'ku vkSj jktLo (iwath] enksa fofHkUu nkSjku ds 2017&18 o"kZ foÙkh; xfrfofèk;ksa dk leFkZu djus ds fy, Hkh dke dj jgh gSaA dh laLFkku ifj;kstuk usVodZ vkSj çcaèku vkSj lwpuk i'kqèku bdkb;ka& nks vykok] blds gSa% x, cuk;s esa 2013 çkS|ksfxdh tSo i'kq 3) vkSj vkuqoaf'kdh] i'kq laLFkku esa rhu foHkkx& 1) i'kq vkuqoaf'kd lalkèku 2) fy, ds djus çkIr dks vfèkns'k ds laLFkku fuekZ.kA lalkèku çcaèku vkSj uhfrxr eqíksa esa leUo; vkSj {kerk vkuqoaf'kd i'kq 2) vkSj mi;ksx vkSj laj{k.k y{k.k] ewY;kadu] igpku] dh lalkèkuksa vkuqoaf'kd dqôqV vkSj i'kqèku ds ns'k 1) fd gS vfèkns'k O;kid dk laLFkku ykHk vkSj mRFkku ds fy, dke dj jgk eq[; laLFkku gSA C;wjks i'kqvksa vkSj dqôqV fo'ks"k :i ls ns'k esa Lons'kh uLyksa ds lalkèku vkuqoaf'kd i'kq Hkk-Ï-vuq-i-&jk"Vªh; fgUnh [k.M jk- i-vk-la-C;wjks dk;Zdkjh lkjka'k dk ikyu ifjogu vkSj [kkn ds fy, fd;k tkrk gSA i'kq xèks yík[kh }kjk leqnk;ksa ckS¼ x;kA fd;k y{k.khÏr dks vkcknh dh xèks ewy esa {ks= yík[k ds tEew&d'ehj fxjkoV dk,degRoiw.kZdkj.kFkhA (xk; gkbfczM esa dks ?kqeUrq (isLVksjfyTe) ds rgr j[kk tkrk gSA bl {ks= ;kd vfèkdka'k gSA gksrk cM+k ls {ks= daèks Jksf.k gSaA gksrsa lhax Åij vkSj ihNs dh vksj ?kqekonkj gksrsa gSaA dku NksVs gSA gksrk dkyk ;k dRFkbZ xgjk jax gSaA gksrs ds vkdkj eè;e ;kd ds uqcjk gSA tkrk ikyk fy, ds ifjogu vkSj Qkbcj cky [kkn] ekal] nwèk] }kjk leqnk; ckS¼ mUgsa x;kA fd;k y{k.khÏr dks vkcknh ;kd LFkkuh; tEew&d'ehj ds uqcjk&yík[k {ks= esa losZ{k.k ds nkSjku] fy, j[kktkrkgSA ds mís'; ds ekal dsoy dks cdfj;ksa nksuksa gSaA gksrsa gq, yVds dku gSaA gksrs dh jax dkys iydsa vkSj FkwFku jax] dksV esa] cdfj;ksa fcnjh gSaA gksrs gq, yVds vkSj iÙksnkj gksrh gS] tcfd] iydsa Hkwjs ;k dkys jax dh gksrs gSaA dku lQsn uanhnqxZ gSA forfjr esa ftyksa dycqxÊ vkSj chnj ds dukZVd fcnjh tcfd gSa] forfjr esa nko.kxsjs vkSj fy, losZ{k.k fd;k x;k A uanhnqxZ cdjh] fp=nqxZ] rqedqj ds fo'ks"krk dh cdfj;ksa fcnjh vkSj uanhnqxZ esa dukZVd vksfM'kk HkSalksadksvyx&vyxlewgksaesaçdVdjrsgSaA y{k.k c‚;ksehfVªd fofHkUu }kjk fo'ys"k.k Hksn gSA jgh cu dkj.k dk Çprk vkcknh ?kVrh dh HkSalksa laHkyiqjh esa bykds ns'kh gSA tkrh ikbZ esa ftyksa ds NÙkhlx<+ ds vklikl vkSj ftys lEHkyiqj ds mM+hlk HkSal laHkyiqjh gSA gksrk rd fnu çfr fdxzk 4 ls 2 mRiknu nwèk gSaA dBksj ysVsjkbV feêh esa [ksrh ds fy, ;s mRÏ"V i'kq gksrs dk jax gYdk dkyk&Hkwjk gSA lÈx yacs vkSj Hkkjh gksrs gSaA gSA eank NksVs dn vkSj lqx<+ dkBh dh gksrh gSA 'kjhj tkrh ikbZ esa ftys dksjkiqV ds vksfM'kk eank x;kA fd;k losZ{k.k dk HkSalksa laHkyiqjh vkSj eank esa jkT; vksfM'kk vU; rjhdksalsloZJs"Bçn'kZufd;kA us e‚My ,loh,e~ esa] 1&ls&vU; vkSj foHkktu 1&ls&1 f}vkèkkjh x;kA fd;k oxÊÏr ij ds y{k.k vkèkkj eksQksZesfVªd lkr dj mi;ksx dk rjhds ;kd) dh çkFkfedrk ;kd la[;k esa la[;k ;kd çkFkfedrk dh ;kd) ´ jk-i-vk-la-C;wjks

85 okf"kZd izfrosnu 2017-18 dk;Zdkjh lkjka'k 86 okf"kZd izfrosnu 2017-18 dk;Zdkjh lkjka'k Årdksa dk mi;ksx vVSpesaV lsy dYpj fofèk ls lsy ls fofèk dYpj lsy vVSpesaV mi;ksx dk Årdksa dku ds ÅaV dPNh vkSj ?kksM+s ef.kiqjh xbZA dh mRiUu ls Ropk ykbu dksf'kdk QkbczksCykLV vkSj dh ?kksM+s ÅaV dPNh ef.kiqjh fy, ds cSaÇdx dksf'kdk nSfgd [kqjkd dh dksf'kdk Øk;ksfçtosZ'ku dsfy,tksM+hx;hA nSfgd 400&400 ls ?kksM+s ekjokM+h vkSj ÅaV dh dPNh vkSj uLyksa [kqjkd oh;Z i'kq 23]975 8 dqy gSA jgk tk fd;k etcwr ls laj{k.k esa dksf'kdkvksa) nSfgd vkSj 'kqØk.kqvksa vfèko`"k.kh Mh,u,] Hkzw.k] (oh;Z] :i fofoèk dks teZIykLe esa cSad thu jk"Vªh; ds C;wjks lalkèku vkuqoaf'kd i'kq jk"Vªh; gksrk gSAcSyesadwcM+eè;evkSjxk;ksaNksVk NksVk ls xksoa'k vksaxksy 'kjhj gSA gksrk eq[; psgjk eq[; gksrs gSaA 'kjhj NksVk vkSj csyukdkj gksrk gSA Hksn ds fy, Hkwjs vfèkdrj >kjh gSA gksrk rd fdyks 2-5 ls 1-0 dk;ks± ds fy, cSyksa dk mi;ksx fd;k tkrk gSA nSfud nwèk Ïf"k gSaA gksrs ?kqekonkj vkSj yacs lÈx gSA gksrk vory vkSj yack psgjk gSA jax lQsn oankjoh dk ftys okjaxy x;kA fd;k losZ{k.k fy, ds o.kZu y{k.k ds dEek) vkSj tjh (oankjoh] vkcknh i'kq Kkr de ds jkT; rsyaxkuk fd;k tkrkgSA ikyu fy, ds (dqjckuh) vuq"Bkuksa ls :i eq[; bldk gSA fo'ks"krk dh nqEck teko ds olk esa iwaN gSaA gksrs lQsn vkSj Hkwjk gksrk gSA dku vkdkj esa cM+s] yVdunkj vtesj ftyksa esa ik;h tkrk gSA vfèkdrj dksV dk jax x;kA ;g eq[; :i ls jktLFkku ds t;iqj] ukxkSj vkSj nqEck HksM+ ds forj.k vkSj fo'ks"krk ds fy, losZ{k.k fd;k 1-5 ls2fdyksxzkegSA mRiknu Åu fpduk okf"kZd gSaA gksrs lhjs'ku ds vkdkj fofHkUu ij Nksj nwjLFk ds dku gS] gksrk iSp xgjk ij gksrh gSaA dksV jax lQsn gksrk gS] vka[kksa] FkwFku vkSj dku Åu çdkj dh HksM+ gSA fprjaxh vkdkj esa eè;e ls cM+s dkyhu ,d ;g x;kA fd;k losZ{k.k dk HksM+ fprjaxh esa Q+ftydk eqälj] ds iatkc Jhxaxkuxj] ds jktLFkku vkbZ gSA esa deh ds dkj.k xèks dh vkcknh dkQh gn rd fxjkoV mi;ksfxrk gSaA l{ke esa djus VªSd ls vklkuh Hkh esa jkr tkuoj gSA gksrs lQsn ;k jax gYds ij isV vkSj dRFkbZ Hkwjs] xgjk ls gYds jax gSaA gksrs 'kkar vkSj vkdkj eè;e jk-i-vk-la-C;wjks jk- i-vk-la-C;wjks ?kVdksa vkSj 117 vk.kfod dk;ks± ds fy, oxÊÏr fd;k dks 132 thu vksaVksykWth tSfod çfØ;k] 146 lsyqyj vkSj 602 fuEu fofu;fer FksA lHkh fofHkUu O;ä thuksa fofu;fer mPp 1236 esa ckaMwj ls buesa pykA irk ftlls dqy 20568 vyx&vyx dadky O;ä VªkafLØIV dk dh HksM+ LFkkuh; ekalisf'k;ksa dk VªkalfØIVksfed çksQkby irk fd;k x;k dh dukZVd vkSj ckaMwj tks gsVjksT;xksV~ldhla[;kesadehn'kkZrhgSA (bufczÇMx vuqeku) Øe'k% 0-096 vkSj 0-043 Fkh] 243 vkSj ,QvkbZ,l vkSlr xbZA dh 237 igpku dh ,yhyksa fof'k"V Øe'k% ,oa x;k fd;k fu:i.k vkuqoaf'kd ls ekdZjksa 24 dk HksM+ nqEck vkSj fprjaxh vfèkd FkhA feyrk gSA fpfydk esa uLy fof'k"V gSIyksVkbi dh la[;k ladsr dk lk>kdj.k dk HkSalksa nyny vkSj fpYdk ls usVodZ esfM;u x;kA ik;k feJ.k dk HkSal esa dkykgkaMh ijy[keqaMh gSaA fof'k"V lcls eqjkZg vkSj eank fd pyk irk ls fo'ys"k.k lajpuk FkhA vyx cgqr ls lHkh HkSal eank fd fn;k ladsr us ekdZjksa ekbØkslkbVscy ij vkèkkj ds fo'ys"k.k MsVk ds vkcknh HkSal vksfM'kk ikap ik;k x;kA FkhA 2 Mh leUo; fo'ys"k.k esa nksuksa cdfj;ksa dks vyx vuqifLFkr ckèkk vuqokaf'kd gkfy;k ;k cM+h Hkh dksbZ gqbZA iqf"V dh Lrj i;kZIr ds fofoèkrk esa vkcknh nksuksa x,A ik, esa ikWfyekWfQZd ekdZj lHkh esa cdfj;ksa fcæh vkuqoakf'kd fo'ys"k.k ds nkSjku dukZVd ds uanhnqxZ vkSj LokLF; f'kfojHkhvk;ksftrfd,x,A nks gqvkA çkIr rd fdyksxzke 4-0 ls 1-0 esa gfj;k.kk gq,A lkfgoky esa nSfud nwèk 4-0 ls 9-0 fdxzk] tcfd iSnk cNM+s 46 dqy x;kA fd;k xHkkZèkku Ïf=e dks lkfgoky 4 vkSj gfj;k.kk 52 dqy x;hA pquh fy, ds laj{k.k vkSj lqèkkj vuqokaf'kd ds xk;ksa lkfgoky vkSj gfj;k.kk xks'kkyk,¡ rhu dh gfj;k.kk vUrxZr ds lrr lqèkkj ds ekWMy ds fodkl ds fy, ,d ifj;kstuk lkFk&lkFk ds laj{k.k ds xk;ksa Lons'kh esa xkS'kkykvksa Øk;ksfçtosZ'ku fd;kx;kA dks dksf'kdkvksa A xbZ dh LFkkfir ls rduhd dYpj QkbczksCykLV fof'k"V ehfM;k dk mi;ksx dj ,DlisykUV dYpj çkFkfed x;kA fd;k fy, ds LFkkiuk dh ykbuksa fgUnh [k.M ds vykok 13 Kkr :iksa esa ls dsoy ch laLdj.k rhuksa laLdj.k ch dsoy ls esa :iksa Kkr 13 vykok ds vkSj 2 , @ 1 fons'kh , x;kA yxk;k vuqeku dk xksoa'k ØkWlczsM ewy] Hkkjrh; çksQkby thuksVkbfid vkSj ,fyfyd fy, ds :iksa Kkr 13 ds dsflu chVk vkSj ekjokM+hesadelsikbZx;hA T;knk lcls esa jsM eækl vfHkO;fä thu dh ,p,lih dks x,A lkaærk ik, thuksVkbi ij yksdl 2 dh okys djus çHkkfor gkeksZu 3 Vh esa HksM+ksa bu x;kA fy, ik;k ds djus çkIr ykHk pqÇunk fy, xbZ ds nkSjku lfg".kqrk ds thuksVkbÇix dh yksdl p;fur 5 fy, ds ,l,uih x;kA fd;k rgr ds fLFkfr rukoxzLr vkSj lkekU; ewY;kadu dk çksQ+kby dk;kZRed dh thu pksdyk] exjk] ekjokM+h vkSj eækl jsM HksM+ esa ,p,lih gq,A O;ä vyx&vyx thu 646 dqy esa ?kksM+ksa taLdkjh nkSjku ds vH;kl x;kA fd;k mRiUu nkSjku ds vH;kl vkSj igys ls vH;kl fo'ys"k.k VªkafLØIVkse dk lh ,e~ ch ih fy, ds djus igpku dh thuksa O;ä nkSjku ds ijh{k.k lgu'khyrk ij ÅapkbZ mPp dh ?kksM+ksa taLdkjh flfôeh ;kdvkcknhvyx&vyxik,x,A vkSj yík[kh FksA okys xq.koÙkk mPp lkFk ds dojst ds 9-42þ ,l,uih 5797575 feykdj] dqy FksA 256051] 241934] 312518 vkSj 150425 dh xbZA vkjMh 10 esa igpkus x, dqy ,l,uih Øe'k% &v#.kkpyh] fgekpyh] yík[kh vkSj flfôeh esa igpku thukse foLr`r ,l ,u ih vkSj bUMsYl pkj ;kd vkcknh ladj lkaMksaesadkQhvfèkdikbZx;hA okys xfr'khyrk mÙke vkSj lfgoky ,p,Q] vfHkO;fä rqyuk esa ckmy] Mh , th ,y vkSj ch oh ,p thu dh dh fÝtoky okys xfr'khyrk oh;Z fuEu fy,] ds djus feFkkbys'ku ik;k x;kA dk;kZRed vfHkO;fä Mh,u, dk vkdyu mPp esa thu ihvkbZohvkbZ,y1 vkSj choh,p Mh,tsM,y] ds fÝtoky lkFk ds xfr'khyrk 'kqØk.kq ds feFkkbys'ku çksQ+kby dk vkdyu fd;k x;kA [kjkc (fÝtoky) ds 'kqØk.kq esa vèkZlw=hfoHkktu ds fof'k"V thu ladj ,p,Q vkSj ,p,Q lfgoky] fy,] ds djus irk Hkwfedk dh feFkkbyhdj.k esa {kerk çtuu i'kq ladj dk irkpykA tk ldk A ekal dh xq.koÙkk ds fy, çklafxd çeq[k ekxks± fgUnh [k.M jk- i-vk-la-C;wjks ldsZfM;u fofu;eu ( fofu;eu ldsZfM;u lZ Syski t,1] kYVu gSIyksVkbi gksYLVhu deh dh dksysLVªkWy gksYLVhu (ts,p1)] ,p,p4] vkSj &,p,p3 gSIyksVkbi tlhZ Mh uksoksvlsacyhHkhdhxbZA dkÆM,d esa dSfY'k;e fofu;eu ( fofu;eu dSfY'k;e esa dkÆM,d ,u ,WQ+ ladsru ekxZ ( ekxZ ladsru ,WQ+ ,u fofu;fer thu ,e~ ,s ih ds ladsru ekxZ ( ekxZ ladsru ds ih ,s ,e~ thu fofu;fer mRiUu fd;k x;k A nqXèkdky ds fofHkUu pj.kksa esa mPp VªkafLØIVkse fy, ds pj.kksa fofHkUu ds nqXèkdky ds HkSalksa ckn] vuqekfurewY;dkvuqekuyxk;ktkldrkgSA ds miyCèkrk dh çHkko ,l,uih x;kA yxk;k vuqeku çHkkoksa ds vkèkkj ij nqXèk xq.kksa ds fy, ,l,uih Cyi vkSj Cyw FksA dk tqM+s ls etcwrh lkFk ds xq.k mit nwèk esa {ks= thuksfed ds vklikl fd pyk irk ls Iy‚V y{k.kksa ij Qsuksfed MsVk ds vkèkkj ij ds dh x;hA eSugêu nwèk vkSj dh ekdZjksa ,l,uih lkaMksa ds larkuksa HkSal 12 1763 vè;;u ,lksfl,'ku okbM thukse {ks=okj }kjkvuqefrnhA ls :i eq[; esa] lewgksa X;kjg dks lewg ds vkcknh lHkh us fo'ys"k.k vuqokaf'kd LFkkfud FkhA feJ.k dh Lrj mPp fy, ds vkcknh HkSal vfèkdka'k dh ns'k NksM+dj dks ef.kiqjh vkSj vle rksMk] esglk.kk] fpfydk] x;kA fd;k fo'ys"k.k dk ,l,uih 23]306 dqy fy, ds igpku dh feJ.k chp ds vkcknh @ uLyksa HkSal 17 ekufp=.k dsfy,vko';dfeysA ,lksfl,'ku 1]20]000 fy, ds HkSalksa nyny vkSj 1]50]000 fy, ds unh la[;k& dh ,l,uih x;hA ikbZ CykWd nwjh tsusfVd dh dsch 25&50 HkSalksa fy, nyny ds vkSj dsch 20&25 fy, ds HkSalksa ds unh gq,] ekurs 2 vkj vfèkd ls 0-2 A x, fd, thuksVkbi ,l,uih 23]306 dqy x;kA fd;k fy, dk tsuksVkbÇix ds yxkus vuqeku dk fMlDohfyfcz;e Çydst mi;ksx }kjk MsVk (thch,l) lhDosaÇlx mRiUu esa tkuojksa 625 ds @vkcknh uLy HkSal 17 fy, ,d=fd,x,A ijh{k.kksa dks iwjk fd;k x;k vkSj uewus vkxs fo'ys"k.k ds esa chlh,e7 vkSj chlh,e9 ds çHkko dk irk yxkus ds çxfr) (eèkqesg pwgs x;kA fd;k Hkh fo'ys"k.k vfHkO;fä xksoa'k esa ik;k x;kA dj.kÝht ds nwèk esa chVk dsflu dk II cuke II cuke I ) FksA HkSal çfrys[kksa dh çfrys[kksa HkSal FksA ) I III ) vkSj O;k;ke çsfjr O;k;ke vkSj ) cuke jk-i-vk-la-C;wjks III II cuke )] ch Mh ch )] I )]

87 okf"kZd izfrosnu 2017-18 dk;Zdkjh lkjka'k 88 okf"kZd izfrosnu 2017-18 dk;Zdkjh lkjka'k d j s x.l= k" i; xk b lo ds lsok bl x;kA ik;k nks"k xq.klw= esa x,A uj fd, LØhu ,d uj 142 dqy fy, ds chekfj;ksa vuqokaf'kd x,A nks"kksa fd, LØhu Øksekslksey uj 344 o"kZ dqy fy, xr ds gSA djrk çnku lsok,a fy, ds yxkus irk ds Øksekslksey chekfj;ksa vuqokaf'kd ,oa dks nks"kksa ,tsafl;ka ljdkjh fofHkUu laLFkku vkSj ,p,p3dhLØhÇuxdsfy,fodflrfd,x,A ts,p1 esa xksoa'kksa çksVksd‚y vkèkkfjr ihlhvkj pysA irk esa lewgksa vuqokaf'kd gksYLVhu vkSj tlhZ Øe'k% esa fLFkfr gsVsjkstkbxl ,p,p4 vkSj ts,p1 & ,yhy mRifjorÊ nks x;kA fd;k fu:fir esa xksoa'kksa Hkkjrh; dks yksdl ds jksxksa vkuqokaf'kd ?kkrd fofHkUu lfgr nku v;st d x± lLku s y vyx vyx us laLFkku xb±A dh vk;ksftr çn'kZuh lkr ij LFkkuksa fofHkUu fy, ds cukus laosnu'khy dks fdlkuksa esa ckjs ds ykHkksa ds tkuojksa Lons'kh vkSj djus çnÆ'kr dks xfrfofèk;ksa dh laLFkku iSnkA tkx:drk chp ds fdlkuksa vkSj fd;k nkSjk dk xkaoksa fofHkUu vkSj fy;k Hkkx esa xkSjo esjk xkao esjk us oSKkfudksa ds C;wjks çf'k{k.k dk;ZØevk;ksftrfd,x,A vkSj lgk;d deZpkfj;ksa ds dkS'ky dks c<+kus ds fy, nks rduhdh ç'kklfud] ds laLFkkuksa vkbZlh,vkj x;kA fd;k vk;ksftr çf'k{k.k dk dkslZ VªsÇux ekWMy vkSj dkslZ 'kkWVZ fy, ds deZpkfj;ksa vdknfed vkSj oSKkfud vkSj iqLrdsa eksuksxzkQ Hkhçdkf'krfd,x,A ikap }kjk laLFkku x,A fd, çdkf'kr ys[k yksdfç; @ rduhdh 12 x,A fd, çdkf'kr i= 'kksèk 33 dqy esa if=dkvksa varjkZ"Vªh; vkSj jk"Vªh; ifj;kstuk,a gSaA 'kksèk 22 lfgr ifj;kstukvksa Qsyks jk"Vªh; ,d vkSj iksf"kr foÙk ckgjh ikap jgs py esa laLFkku xbZA esa orZeku dh iwjh ifj;kstuk,a 'kksèk 7 dqy lky fiNys lajf{kr fd;ktkjgkgSA esa vkokl muds dks uLyksa 2 vkSj x;h pquh vkcknh ubZ 9 fy, ds y{k.khdj.k rgr] ds çkstsDV usVodZ çkIr gq,A esa :i ds jktLo 8]30]500@& #- dks laLFkku }kjk jk-i-vk-la-C;wjks jk- i-vk-la-C;wjks

  LFkkukarfjr vkSj,dlsokfuo`r gq,A nks 'kkfey] esa laLFkku deZpkjh nks gqbZA çkIr inksUufr esa xzsM mPp vxys vius dks deZpkfj;ksa rduhdh pkj ç'kklfud vkSj 4 dq'ky deZpkjh gSaA nks oSKkfudksa vkSj orZeku esa laLFkku esa 29 oSKkfud] 16 rduhdh] 17 vuqlUèkku laLFkkuHkh'kkfeygSA ftuesa ekLVlZvkSjih,pMhfMxzhdsfy,us'kuyMs;jh gSa] jgs dj ekxZn'kZu dks Nk=ksa ds foÜofo|ky;ksa laLFkkuksa vkSj fofHkUu ls :i dk;Z fu;fer oSKkfud dk gSaA i<+kus djrs dks ikBîØeksa fofHkUu esa bu fMohtuksa vkSj gS x;k fd;k dh 'kkfey esa jlk;u ladk; LukrdksÙkj tSo i'kq vkSj ck;ksVsDuksykWth ,fuey çtuu] vkSj vkuqoaf'kdh i'kq ds laLFkku vuqlUèkku Ms;jh Hkk-Ï-vuq-i-&&us'kuy dks oSKkfudksa ds C;wjks vkSj dk;ZØeksa çf'k{k.k dk;Z'kkykvksa] laxks"Bh]lEesyuksaesaHkkxfy;kA Hkhrj ds ns'k us oSKkfudksa rFkk O;fä;ksa uslaLFkkudknkSjkfd;kA vkbZlh,vkj egkfuns'kd] miegkfuns'kd (i-fo-)] vkbZlh,vkj o lfgr dbZ çfrf"Br Ms;j lfpo [ksyksa esaHkkxfy;kA ij fofHkUu ds 2018 & izfr;ksfxrk [ksy okÆ"kd vk;ksftr volj ds fnol x.kra= us deZpkfj;ksa ds C;wjks vkSj ckLdsVckWy igq¡phA rd nkSj lsehQkbuy Vhe LeSÇ'kx o‚yhc‚y fy;kA Hkkx esa [ksyksa {ks=) (mÙkjh vkbZlh,vkj us ny ,d ds deZpkfj;ksa 22 ds laLFkku vkSj vuqlaèkku ifj;kstukvksadhçxfrleh{kkxbZA xbZ dh vk;ksftr ij le; lfefr lykgdkj laLFkku vuqlaèkku lfefr (vkbZvkj,lh) vkSj vuqlaèkku vkSj Hkkjrh;if=dk,a'kkfeydhxbZA fons'kh 23 esa if=dkvksa fofHkUu esa] iqLrdky; C;wjks bR;kfn euk;sA i[kokM+k Çgnh fnol] f'k{kk Ïf"k fnu] ;ksx varjkZ"Vªh; lIrkg] lrdZrk vfHk;ku] LoPNrk jk"Vªh; foKku] t; fdlku t; fnol] Lora=rk vkSj fnol x.kra= fnu] volj tSls dh C;wjks dk LFkkiuk fnol] tSo fofoèkrk fgUnh [k.M 15-2-2018 dksvk;ksftr dhxbZgSaA rFkk 14-9-2018 esa çfØ;k blh gS- tkrk fd;k vk;kstu dk O;k[;kuksa@dk;Z'kkykvksa fganh gsrq nsus nSfud jktdh; dk;ks± esa vfèkdkfèkd ç;ksx dks çxfr laLFkku esa jktHkk"kk fganh ds çpkj&çlkj rFkk blds vk;kstu laLFkku esa fganh O;k[;ku@dk;Z'kkykvksa dk 08-8-2017 dksfd;kx;kA rFkk 27-7-2017 vk;kstu dk cSBd dh lfefr lykgdkj jktHkk"kk gSA tkrh cuk :i&js[kk dh dk;ZØeksa djds ppkZ ij fo"k;ksa lacafèkr vk;kstu ds i[kokM+k vFkok ekg psruk fganh lacafèkr] ls çdk'ku ds çdk'kÞ Þi'kqèku if=dk fganh okf"kZd dh laLFkku lfefr dh cSBdksa dk vk;kstu Hkh fd;k tkrk gS ftles laLFkku esa le;≤ ij laLFkku jktHkk"kk lykgdkj cSBdksa dkvk;kstu dh lfefr lykgdkj jktHkk"kk laLFkku 26-3-2018 dksvk;ksftrdhxbZgSaA 2-5-2017] 29-7-2017 19-1-2018 rFkk cSBdsa nks rd] vHkh nkSjku ds vofèk bl gSA tkrh frekgh esa fu"ikfnr dkjZokbZ ij ppkZ djds iqf"V dh çR;sd gSA tkrk fd;k vuqikyu o fØ;kUo;u ij fu.kZ;ksa x, fy, esa cSBdksa bu gSaA tkrh dh fujarj cSBdsa dh lfefr dk;kZUo;u jktHkk"kk esa laLFkku cSBdsa dh lfefr dk;kZUo;u jktHkk"kk laLFkku fgUnh [k.M jk- i-vk-la-C;wjks jktHkk"kk izdks"Bdhxfrfof/k;k° gsrq fnukad 12-9-2017 dks Þesjk xkao esjk xksjoß C;wjks ds oSKkfudksa@rduhdh vfèkdkfj;ksa@'kksèkosÙkkvksa }kjk djdsfotsrkvksadksiqjLÏr fd;kx;kA ls fganh esa fd;s x, dk;ks± dk ewY;kadu xfBr lfefr esa dk;ks± jktdh; x;s fd;s nkSjku ds 2016&17 gsrq p;u ds fd;k dkfeZd mRÏ"V fganh esa laLFkku vk;kstu x;k- dk çfr;ksfxrk@dk;Z'kkyk Kku 'kCn fganh rhu fnol ls :i fo'ks"k fganh ij volj 'kqHk dks ds 14-9-2017 fnukad x;kA fd;k vk;kstu dk Fkk] Hkkjrß jfgr Þudnh fo"k; ftldk çfr;ksfxrk Hkk"k.k Hkk"k.k] vk'kq çfr;ksfxrk] vuqokn o 'kCnkFkZ ,oa çfr;ksfxrk ys[ku elksnk blds vfrfjä fganh i= ys[ku çfr;ksfxrk] fVIi.kh@ fo"k; xks laj{k.k esa xkspj.k dk egRo j[kk x;k FkkA çfr;ksfxrk dk vk;kstu fd;k fucaèk x;kA fganh fucaèk ys[ku varxZr dk ds çfr;ksfxrkvksa ys[ku fganh dk lapkyufd;kA C;wjks LVkQ ds fy, fyf[kr o ekSf[kd çfr;ksfxrkvksa us vfèkdkjh jktHkk"kk lriky] Jh lfpo lnL; feJ] MkW- lksfudk vgykor] MkW- dj.kohj flag vkSj vfuy MkW- lnL;x.k rFkk oSKkfud çèkku oekZ] ds- fganh ,u- MkW- xfBr vè;{k ftlds lfefr }kjk vk;kstu i[kokM+k egksn; funs'kd x;kA fd;k vk;kstu dk i[kokM+s fganh rd 2017 flrEcj] 6&21 Hkh o"kZ bl Hkkafr dh o"kZ çR;sd esa C;wjks fooj.k laf{kIr dk vk;kstu ds i[kokM+k fganh jk-i-vk-la-C;wjks

89 okf"kZd izfrosnu 2017-18 jktHkk"kk izdks"B dh xfrfof/k;k¡ 90 okf"kZd izfrosnu 2017-18 jktHkk"kk izdks"B dh xfrfof/k;k¡ oook; ej u fah f;sxkka ds çfr;ksfxrkvksa fotsrkvksa fganh dks iqjLdkj çnku fd;sA blh 'kqHk volj us esjB fo'ofo|ky;] vuqlUèkku xks ,oe~ foKku i'kq mikè;k; nhun;ky gh nkSjku eq[; vfrfFk MkW- ds-,e-,y- ikBd] dqyifr] iafMr ds lekjksg fd;kA vk;ksftr lekjksg viuk forj.k iqjLdkj fganh us rFkk fnol LFkkiuk laLFkku 34oka dks 21-9-2017 fnukad iqjLdkj forj.klekjksg vk;kstu fd;kx;kA dk çfr;ksfxrk çLrqrhdj.k 'kkSèk&i= esa fganh dks 13-9-2017 fnukad rFkk x;k fd;k vk;kstu dk çfr;ksfxrk iksLVj&çn'kZu ys[k fganh ij fo"k; jk-i-vk-la-C;wjks jk- i-vk-la-C;wjks   iqjLdkj çnkufd;sx,A Hkh dks ys[kksa Js"B rhu ij vkèkkj ys[kksa ds ewY;k¡du ds çdkf'kr esa 2016) (o"kZ vad lIre ds çdk'kß Þi'kqèku if=dk fganh okf"kZd dh C;wjks ij fo'ks"k miyfCèk foekspu Hkhfd;kA dk 2017) (o"kZ vad v"Ve vuqlUèkku ds çdk'k i'kqèku xks ,oe~ if=dk fganh okf"kZd dh C;wjks us esjB fo'ofo|ky;] foKku i'kq mikè;k; nhun;ky iafMr dqyifr] ikBd] ds-,e-,y- MkW- vfrfFk eq[; nkSjku ds lekjksg forj.k iqjLdkj vad dkfoekspu okf"kZd fganh if=dk i'kqèku çdk'k ds v"Ve çkIr gqvkA i= ç'kfLr rFkk VªkQh ,d fy, ds laLFkku ftles feyk] dks laLFkku gekjs nkSjku ds cSBd Nekgh dh f}rh; iqjLdkj fnukad 09-6-2017 dks ujkdkl dk 2016&17 o"kZ varxZr ds iqjLdkjksa jktHkk"kk uxj jktHkk"kk dk;kZUo;u lfefr }kjk fn, tkus okys fgUnh [k.M