Product Data Sheet APC anti-human CD143 (Angiotensin-converting )

Catalog # / Size: 2321030 / 100 tests Clone: 5-369 Isotype: Mouse IgG1, κ Reactivity: Human Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody. Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). Concentration: Lot-specific GM-CSF-stimulated human peripheral blood mononuclear cells (day-3) were stained with CD143 (clone 5-369) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram). Data shown was gated on CD14+ cell population.

Applications:

Applications: Flow Cytometry Recommended Each lot of this antibody is quality control tested by immunofluorescent staining Usage: with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Description: CD143 (ACE, kininase II, peptidyl 1, peptidase P, carboxycathepsin) is a 171 kD, type I, single chain transmembranal metallopeptidase, whose is zinc. Its main targets are angiotensin I and bradykinin, acting as a blood pressure regulator. CD143 is expressed in endothelial cells; varying amounts of CD143 have been reported in different epithelial cells. The activation of macrophages and histiocytes induces the expression of this molecule. Antigen 1. Nakamura T, et al. 2009. Int Heart J. 50:501. References: 2. Jayasooriya AP, et al. 2008. P. Natl. Acad. Sci. USA 105:6531. 3. Jokubaitis VJ, et al. 2008. Blood 111:4055. 4. Arndt PG, et a

For research use only. Not for diagnostic use. Not for resale. Sony Biotechnology Inc. will not be held responsible for patent infringement or other violations that may occur with the use of our products. Sony Biotechnology Inc. 1730 North First Street, San Jose, CA 95112 www.sonybiotechnology.com