Effects of Dietary Feeds on the Growth Performance and Survivability of Stinging Catfish, Heteropneustesfossilis (Bloch, 1794) Fingerlings”

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Effects of Dietary Feeds on the Growth Performance and Survivability of Stinging Catfish, Heteropneustesfossilis (Bloch, 1794) Fingerlings” “Effects of Dietary Feeds on the Growth Performance and Survivability of Stinging Catfish, Heteropneustesfossilis (Bloch, 1794) Fingerlings” BY Examinations roll no. 605 Session: 2008-2009 Registration no. HA-912 A thesis submitted in the partial fulfillment of the requirements for the degree of Master of Science (MS) in Fisheries Previous degree: Bachelor of Science (Hon’s) in Fisheries Department of Fisheries University of Dhaka Department of Fisheries University of Dhaka Dhaka-1000, Bangladesh Dhaka University Institutional Repository CERTIFICATE This is to certify that this thesis entitled, “Effects of Dietary Feeds on the Growth Performance and Survivability of Stinging Catfish, Heteropneustesfossilis (Bloch, 1794) Fingerlings” submitted by Md. JonayedHossain, Exam. Roll: Curzon- 605, Session: 2012-2013, Registration No.: HA-912, Session: 2008-2009 has been carried out under our supervision. This is further to certify that this is an original work and suitable in partial fulfillment for the degree of MS in Fisheries, University of Dhaka. Anwar Hossain Shankar Chandra Mandal Assistant Professor Assistant Professor Department of Fisheries Department of Fisheries University of Dhaka University of Dhaka Dhaka-1000, Bangladesh Dhaka-1000, Bangladesh Md. AlamgirKabir Lecturer Department of Fisheries University of Dhaka Dhaka-1000, Bangladesh Dhaka University Institutional Repository Acknowledgements All praises belong to the Almighty Allah, who is the most gracious and the most merciful, for successful completion of the work. I express heartfelt gratitude and appreciation to my reverent teacher and research supervisor Anwar Hossain and Shankar Chandra Mandal, Assistant Professor, Department of Fisheries, University of Dhaka; for his incessant supervision, designing the study, guidance and constructive criticism throughout the work. I am also beholden to him for his boundless patience for careful reading and reviewing the write-up. I also express my heartfelt feelings for my co-supervisor Md. AlamgirKabir, Lecturer, Department of Fisheries, University of Dhaka for his kind assistance to complete my thesis. I am also indebted to Mrs. WahidaHaque, Chairperson, Department of Fisheries, University of Dhaka for giving me the opportunity to use departmental laboratory, and also to Dr. Md. GhulumMostafa and Dr. Mahmud Hasan, Professor, Department of Fisheries, University of Dhaka for their encouragement and assistance to complete the work. Special thanks to Mr. Mohammad MamunChowdhury, Associate Professor, also to Dr. Mohammad ShamsurRahman, Assistant Professor, and all other teachers of Department of Fisheries, University of Dhaka. Special thanks must go to my beloved friend, Mahmud for his outmost help during laboratory works and continuous encouragement. I am also delighted to express my wholehearted feelings of thankfulness and gratefulness to Lab attendances of the Department of Fisheries. March, 2014 The Author i Dhaka University Institutional Repository ABSTRACT Stinging catfish (Heteropneustesfossilis) is a highly nutritious food and contribute to the fulfillment of animal protein. Now a day, this fish are commercially cultured through the country. Fish feeds play important roles in growth, survival and development of stinging catfish. But due to inadequate knowledge on effects of feeds on growth, survival and development of stinging catfish, mass and quality production of this fish is not possible. Thus the present study was conducted to evaluate the effects of three different feeds on growth, survival and development of stinging catfish. There were three types of feeds used including Live Tubifex, Tubifexpowder (58% protein) and Pellet feed (20% protein) in the experiment. Feeding rates were evaluated (5%), as a percentage of fish body weight, with three replicates per treatment. All fingerlings were of the same age at stocking; with a mean weight of 8.04 ± 0.01 g. The fishes were fed twice per day at 9.30 to10.30 a.m. and 3.30 to 4.30 p.m. respectively. At the beginning of the experiment and at 7 day intervals all the fish from each aquarium were collected, counted, individually weighed (nearest mg) for 45 days. Final weights were significantly greater (p< 0.05) than initial weights in the entire feeding rate. After 45 day rearing, the mean harvesting weights of stinging catfish, Shingi were 48.53 ± 1.13g, 28.97 ± 0.24g and 25.67 ± 0.49g respectively in T1, T2, and T3. Significantly higher mean weight of fish (g) was found in stinging catfish at 45 day fed with live tubificid worm than that of others (P<0.05). The mean weights of stinging catfish, Shingi in three treatments such as T1, T2 and T3 were significantly different (p<0.05) from each other. The best survival was found in T1 (90%) among the treatments fed with live tubificid worm. The best feed conversion ratio (1.5167 ± 0.02028) was observed in T1. Significantly the highest specific growth rate (4.5833 ±0.04631) was obtained in T1.Significantly higher value (17.48 ± 0.28) of protein content was measured in stinging catfish at 45 day fed with live tubificid worm (P<0.05). The objective of the present study was to assess the possibility of improving growth performance of stinging catfish fingerlings with supplemented feeds. As stinging catfish was being grown with several options of feed including three types of feeds, Live Tubifex, Tubifexpowder and Pellet feed were tested. ii Dhaka University Institutional Repository Table of Content Chapter Title Page Acknowledgements i Abstract ii Contents iii List of tables vi List of figures vii 1 INTRODUCTION 1.1 General Background 1 1.2 Rationale 2 1.3 Problem statement 3 1.4 Research needs 3 1.5 Objectives 4 2 MATERIALS AND METHODS 5 2.1 Experimental fish 5 2.2 Scientific classification of Heteropneustes fossilis, 5 (Bloch, 1794) 2.3 Collection of Samples 6 2.4 Study Place 6 2.5 Study period 6 2.6 Experimental Design 6 2.7 Feeding trial of fish 7 2.8 Apparatus and Materials 7 2.9 Experimental feed 7 2.10 Shingifingerling growth trial 8 iii Dhaka University Institutional Repository 2.10.1 Fish sampling Procedure 8 2.10.2 Analysis of experimental data 8 2.10.2.1 Average Daily Gain (ADG, g/day) 8 2.10.2.2 Specific Growth Rate (SGR ,%) 9 2.10.2.3 Feed Conversion Ratio (FCR) 9 2.10.2.4 Determination of growth performance 9 2.11 Water Quality Monitoring 10 2.11.1 Water temperature(ºC) 10 2.11.2 Dissolved oxygen (DO) 10 2.11.3 Hydrogen ion concentration (PH) 10 2.11.4 Conductivity 10 2.11.5 Turbidity in water 10 2.12 Analytical methods for determination of biochemical 10 composition 2.12.1 Sample preparation 11 2.12.2 Determination of Moisture Content 11 2.12.3 Determination of the protein content 12 2.13 Statistical analysis 13 3 RESULTS 3.1 Effects on the growth performance of stinging catfish 18 fingerlings fed with different feed. 3.1.1 Growth Performance 18 3.1.2 Survival rate(%) of stinging catfish fingerlings 19 3.1.3 Average Daily Gain (ADG, g/day) 20 iv Dhaka University Institutional Repository 3.1.4 Specific Growth Rate (SGR) 21 3.1.5 Feed Conversion Ratio (FCR) 22 3.2 Water quality parameters 23 3.2.1 Water temperature(ºC) 23 3.2.2 Hydrogen ion concentration (PH) 24 3.2.3 Turbidity in water 25 3.2.4 Water Conductivity 26 3.3 Proximate Analysis of Fish Samples 27 3.3.1 Moisture Content in Fish 27 3.3.2 Protein Content in Fish 27 4 DISCUSSION 29 5 CONCLUSION AND RECOMMENDATIONS 34 5.1 Conclusion 34 5.2 Recommendations 35 REFERENCES 36 APPENDICES 42 v Dhaka University Institutional Repository LIST OF TABLES Table Title Page No. NO. 1 Weight of fish (g) (Mean ± SEM) of stinging catfish fingerlings at 45 days 18 rearing period. 2 Survival rate (%) (Mean ± SEM)of Stinging catfish fingerlings at 45 days 19 rearing period 3 Average Daily Gain, ADG (Mean ± SEM) of Stinging catfish fingerlings 20 at 45 days rearing period. 4 Specific Growth Rate, SGR (Mean ± SEM) of Stinging catfish fingerlings 21 at 45 days rearing period. 5 Feed Conversion Ratio, FCR (Mean ± SEM) of Stinging catfish 22 fingerlings at 45days rearing period. 6 Water temperature (Mean ± SEM)of Stinging catfish fingerlings at 23 45days rearing period. 7 Hydrogen ion concentration (PH) (Mean ± SEM) of Stinging catfish 24 fingerlings at 45days rearing period. 8 Turbidity in water (Mean ± SEM) of Stinging catfish fingerlings at 45days 25 rearing period. 9 Water Conductivity (Mean ± SEM) of Stinging catfish fingerlings at 26 45days rearing period. 10 Proximate composition (Mean ± SEM) at 45 days rearing period 27 vi Dhaka University Institutional Repository LIST OF FIGURS Title Page Figure No No 1 DO Meter(A), Conductivity Meter(B) And Live Tubificid Worm(C). 15 2 Powder Tubificid worm (D and F) and Pellet Feed (E). 16 3 Fish Sample ( G), Electric Balance (H), Way of taking Measurement( I & J) 17 vii Dhaka University Institutional Repository Chapter 1 Introduction 1.1 General background The first efforts at raising catfish were made in the early 1900's at several federal and state fish hatcheries. In the 1950's commercial catfish farming first started in Kansas and Arkansas. By 1965, there were over 7,000 acres of commercial catfish ponds in Arkansas, along with acreage in Louisiana, Texas, Alabama, Georgia, Oklahoma, and Kansas. From this inauspicious beginning, commercial catfish farming in Mississippi grew rapidly (Giachelli, J. W. and J. E. Waldrop. 1983). Among the air-breathing catfishes, stinging catfish (Heteropneustes fossilis) is very popular and high valued fish in Bangladesh. It is considered to be highly nourishing, palatable and tasty and well preferred because of its less spine, less fat and high digestibility in many parts of Indian subcontinent (Khan et al. 2003). Due to high nutritive value the fish is recommended in the diet of sick and convalescents.
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