SANTA CRUZ BIOTECHNOLOGY, INC. PSAT1 shRNA (h) Lentiviral Particles: sc-92619-V

BACKGROUND APPLICATIONS PSAT1 (phosphoserine aminotransferase 1), also known as phosphoserine PSAT1 shRNA (h) Lentiviral Particles is recommended for the inhibition of aminotransferase, phosphohydroxythreonine aminotransferase, endometrial PSAT1 expression in human cells. progesterone-induced protein, PSA, PSAT, EPIP or MGC1460, is a 370 amino acid protein belonging to the class-V pyridoxal-phosphate-dependent amino- SUPPORT REAGENTS transferase family and serC subfamily. PSAT1 catalyzes the second step in Control shRNA Lentiviral Particles: sc-108080. Available as 200 µl frozen the L-serine synthesis pathway, converting 3-phosphohydroxypyruvate into 3- viral stock containing 1.0 x 106 infectious units of virus (IFU); contains an phosphoserine. While highly expressed in pancreas, brain, kidney and liver, shRNA construct encoding a scrambled sequence that will not lead to the PSAT1 is expressed at very low levels in colon, testis, thymus and prostate. specific degradation of any known cellular mRNA. There are two known isoforms of PSAT1 which result from alternative splicing events, and the gene encoding PSAT1 maps to human chromsome 9q21.2. RT-PCR REAGENTS PSAT1 deficiency can cause phosphoserine aminotransferase deficiency (PSATD), whose symptoms include psychomotor retardation, intractable Semi-quantitative RT-PCR may be performed to monitor PSAT1 gene expres- seizures, acquired microcephaly and hypertonia. sion knockdown using RT-PCR Primer: PSAT1 (h)-PR: sc-92619-PR (20 µl). Annealing temperature for the primers should be 55-60° C and the extension REFERENCES temperature should be 68-72° C. 1. Misrahi, M., et al. 1987. A novel progesterone-induced messenger RNA in BIOSAFETY rabbit and human endometria. Cloning and sequence analysis of the com- plementary DNA. Biochemistry 26: 3975-3982. Lentiviral particles can be employed in standard Biosafety Level 2 tissue culture facilities (and should be treated with the same level of caution as 2. Basurko, M.J., et al. 1999. Phosphoserine aminotransferase, the second with any other potentially infectious reagent). Lentiviral particles are repli- step-catalyzing enzyme for serine biosynthesis. IUBMB Life 48: 525-529. cation-incompetent and are designed to self-inactivate after transduction 3. Baek, J.Y., et al. 2003. Characterization of human phosphoserine amino- and integration of shRNA constructs into genomic DNA of target cells. transferase involved in the phosphorylated pathway of L-serine biosynthe- sis. Biochem. J. 373: 191-200. RESEARCH USE 4. Hart, C.E., et al. 2007. Phosphoserine aminotransferase deficiency: a novel The purchase of this product conveys to the buyer the nontransferable right disorder of the serine biosynthesis pathway. Am. J. Hum. Genet. 80: to use the purchased amount of the product and all replicates and derivatives 931-937. for research purposes conducted by the buyer in his laboratory only (whether the buyer is an academic or for-profit entity). The buyer cannot sell or other- 5. Online Mendelian Inheritance in Man, OMIM™. 2007. Johns Hopkins wise transfer (a) this product (b) its components or (c) materials made using University, Baltimore, MD. MIM Number: 610936. World Wide Web URL: this product or its components to a third party, or otherwise use this product http://www.ncbi.nlm.nih.gov/omim/ or its components or materials made using this product or its components 6. Vie, N., et al. 2008. Overexpression of phosphoserine aminotransferase for Commercial Purposes. PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cells. Mol. Cancer 7: 14. PROTOCOLS See our web site at www.scbt.com for detailed protocols and support CHROMOSOMAL LOCATION products. Genetic locus: PSAT1 (human) mapping to 9q21.2.

PRODUCT PSAT1 shRNA (h) Lentiviral Particles is a pool of concentrated, transduction- ready viral particles containing 3 target-specific constructs that encode 19- 25 nt (plus hairpin) shRNA designed to knock down gene expression. Each vial contains 200 µl frozen stock containing 1.0 x 106 infectious units of virus (IFU) in Dulbecco’s Modified Eagle’s Medium with 25 mM HEPES pH 7.3. Suitable for 10-20 transductions. Also see PSAT1 siRNA (h): sc-92619 and PSAT1 shRNA Plasmid (h): sc-92619-SH as alternate gene silencing products.

STORAGE Store lentiviral particles at -80º C. Stable for at least one year from the date of shipment. Once thawed, particles can be stored at 4º C for up to one week. Avoid repeated freeze thaw cycles.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com