Ye et al
7URSLFDO-RXUQDORI3KDUPDFHXWLFDO5HVHDUFK-DQXDU\ ,661 SULQW HOHFWURQLF 3KDUPDFRWKHUDS\*URXS)DFXOW\RI3KDUPDF\8QLYHUVLW\RI%HQLQ%HQLQ&LW\1LJHULD $YDLODEOHRQOLQHDWKWWS ZZZWMSURUJ KWWS G[GRLRUJWMSUYL 2ULJLQDO5HVHDUFK$UWLFOH /RQJQRQFRGLQJ51$.&14RSSRVLWHWUDQVFULSW .&1427 UHJXODWHVFHOOSUROLIHUDWLRQDQGLQYDVLRQYLD WDUJHWLQJPL5SLQJDVWULFFDUFLQRPD ;LQ\LQJ *For correspondence: Email: [email protected]; Tel: +86-18726520999 Sent for review: 13 April 2020 Revised accepted: 20 September 2020 Abstract Purpose: Long non-coding RNA (lncRNA) KCNQ1OT1 (KCNQ1 opposite strand/antisense transcript 1) has been associated with many kinds of cancers. However, its role hasn’t been researched much in gastric cancer. The present study investigated the role and mechanism of KCNQ1OT1 in gastric cancer. Methods: Quantitative-PCR assays was used to measure KCNQ1OT1 and miR-124-3p expression in gastric cancer tissues and cells. Transfection assays were conducted to evealuate the regulation of KCNQ1OT1 or miR-124-3p expression in vitro. CCK-8 assays examined cell proliferation and Transwell invasion assays checked cell invasion abilities due to the changes of the key genes. On the other hand, luciferase reporter assays assisted in verifying the binding between KCNQ1OT1 and miR-124-3p. To explore the mechanism of KCNQ1OT1 in gastric cancer, we checked the independent influence of .KCNQ1OT1 or miR-124-3p on cellular functions and also examined the interplay between the two genes in gastric cancer cell lines. Results: The findings showed that KCNQ1OT1 had high expression while miR-124-3p had low expression in gastric cancer tissues and cells in comparison with normal ones. KCNQ1OT1 was found to bind and target miR-124-3p. KCNQ1OT1 was shown to promote cell proliferation and invasion while miR-124-3p inhibit. Furthermore, the inhibition of miR-124-3p could partially reverse cell function changes induced by the knockdown of KCNQ1OT1 including cell viability, cell invasion and EMT process. Conclusion: KCNQ1OT1 was a target of miR-124-3p and reduction in its expression led to an increase of miR-124-3p and inhibited the proliferation, invasion and EMT in Gastric Cancer. These results provided evidence that KCNQ1OT1 promoted the growth and invasion of the cells via targeting miR- 124-3p in GC. Keywords: KCNQ1OT1, miR-124-3p, gastric carcinoma, proliferation, invasion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© 2021The authors. 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British journal 7KLV LV DQ 2SHQ $FFHVV DUWLFOH WKDW XVHV D of cancer 2016, 115:1367-1378. IXQGLQJPRGHOZKLFKGRHVQRWFKDUJHUHDGHUVRU 14. Nakano S, Murakami K, Meguro M, Soejima H, WKHLULQVWLWXWLRQVIRUDFFHVVDQGGLVWULEXWHGXQGHU Higashimoto K, Urano T, Kugoh H, Mukai T, Ikeguchi M, Oshimura M: Expression profile of LIT1/KCNQ1OT1 WKH WHUPV RI WKH &UHDWLYH &RPPRQV $WWULEXWLRQ and epigenetic status at the KvDMR1 in colorectal /LFHQVH KWWS FUHDWLYHFRPPRQVRUJOLFHQVHVE\ cancers. Cancer science 2006, 97:1147-1154. DQG WKH %XGDSHVW 2SHQ $FFHVV ,QLWLDWLYH 15. Soejima H, Nakagawachi T, Zhao W, Higashimoto K, Urano T, Matsukura S, Kitajima Y, Takeuchi M, KWWS ZZZEXGDSHVWRSHQDFFHVVLQLWLDWLYHRUJUHD Nakayama M, Oshimura M, et al: Silencing of imprinted G ZKLFK SHUPLW XQUHVWULFWHG XVH GLVWULEXWLRQ CDKN1C gene expression is associated with loss of DQG UHSURGXFWLRQ LQ DQ\ PHGLXP SURYLGHG WKH CpG and histone H3 lysine 9 methylation at DMR-LIT1 in esophageal cancer. Oncogene 2004, 23:4380-4388. 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