VALERIE MIZRAHI

Abridged CV

PERSONAL Born in , , 23/08/58. Citizen of Italy and Permanent Resident of SA

EDUCATION 1983 Doctor of Philosophy (Chemistry), (UCT), 1980 Bachelor of Science (Honours) (Chemistry), UCT, Degree with distinction 1979 Bachelor of Science, (Chemistry & Mathematics), 1979, UCT. Distinctions in Chemistry & Mathematics and degree with distinction 1976 A Levels, Salisbury Girls High School, Zimbabwe (Distinctions in Mathematics, Chemistry and Physics), and S Level (Distinction in Chemistry)

POSTGRADUATE TRAINING & FELLOWSHIP APPOINTMENTS 1983-1986 Postdoctoral Fellow, Pennsylvania State University, State College, PA, USA

FACULTY APPOINTMENTS

2011- Director, Institute of Infectious Disease and Molecular Medicine (IIDMM), University of Cape Town (UCT) 2011- Professor, Faculty of Health Sciences, UCT 2011- Head, UCT Node, DST/NRF Centre of Excellence for Biomedical TB Research 2011- Director, MRC/NHLS/UCT Molecular Mycobacteriology Research Unit 2004-2010 Co-Director, DST/NRF Centre of Excellence for Biomedical Research (CBTBR) 2005- 2010 International Research Scholar of the Howard Hughes Medical Institute (USA) 2002-2010 Research Professor, School of Pathology, University of the Witwatersrand (WITS) 2000-2010 Director, MRC/NHLS//WITS Molecular Mycobacteriology Research Unit (MMRU) 2000- 2005 International Research Scholar of the HHMI, USA 1999-2001 Executive Committee, SA Institute for Medical Research (SAIMR) 1998-2001 Head, Research Grants Administration, SAIMR 1989-2000 Head, Molecular Biology Unit, SAIMR. 1992-2002 Reader in the Field of Molecular Biology, Department of Molecular Medicine & Haematology, WITS 1989-1991 Senior Research Officer, Department of Haematology, WITS 1988-1989 Associate Senior Investigator, Department of Macromolecular Sciences, SmithKline & French R&D, King of Prussia, PA, USA 1986-1987 Chief Researcher, CSIR Laboratory for Molecular & Cell Biology; Honorary Research Fellow, Department of Genetics, WITS

SCIENTIFIC AWARDS, PRIZES AND ACHIEVEMENTS

2010 Division U (Mycobacteriology) Lecturer, American Society for Microbiology

2009 Awarded “A” rating by National Research Foundation of South Africa 2009 Elected as a Fellow of the American Academy of Microbiology 2008 Co-organiser, Keystone Symposium on Pathogenesis and Control of Emerging Infections and Drug-Resistant Organisms, Bangkok, Thailand 2007 Order of the Mapungubwe: Silver, conferred by the State President of South Africa 2007 Chair, Gordon Research Conference on Tuberculosis Drug Development, Oxford 2006 Gold Medal, South African Society for Biochemistry & Molecular Biology 2006 Distinguished Woman Scientist Award, Dept. Science & Technology, South Africa 2006 Shoprite-Checkers/ SABC2 Woman of the Year , Science and Technology Category 2005 Vice-Chair, Gordon Research Conference on Tuberculosis Drug Development 2005 Selected as one of 42 International Research Scholars of the Howard Hughes Medical Institute (HHMI) under the Infectious Diseases and Parasitology Program, 2005-2010 2004 Co-awarded a DST/NRF Centre of Excellence (jointly with Prof. P.D. van Helden) 2003 Selected as one of 16 Jewish women scientists worldwide for inclusion in the Jewish Women Scientists calendar project of the Hadassah International Research Institute on Jewish Women, Brandeis University, USA 2002 Promoted to Research Professor in recognition of achievements in research 2002 Elected as an Associate Fellow of the Third World Academy of Science (TWAS) 2000 Winner, UNESCO-L’ORÉAL ‘ For Women in Science ’ Prize for the Africa and Middle-East Region 2000 Elected as a Fellow of the Royal Society of South Africa 1999 Selected as one of 45 International Research Scholars of the HHMI under the Infectious Diseases and Parasitology Program, 2000-2005 1996 Elected as a Member of the Academy of Science of South Africa 1992 Silver Medal, SA Biochemical Society 1991 President’s Award in Biochemistry, Foundation for Research Development 1991 Winner, Four Outstanding Young South Africans (FOYSA) Award 1990 Watkins-Pitchford Publication Prize, SAIMR 1990 Meiring-Naudé Medal, Royal Society of South Africa 1990 Vice-Chancellor’s Research Award, University of the Witwatersrand 1980-1983 AE&CI Graduate Fellowship, Beit Research Fellowship (Honorary), Myer Levinson Scholarship for Best Science Honours Graduate at UCT, Gordon Percy Memorial Prize for Best Chemistry Honours Graduate at UCT 1977-1979 Roderick Noble Scholarship for Best Science Graduate at UCT and 6 class medals

EDITORIAL POSITIONS 2010- Editorial Board, Cellular Microbiology Pre-2010 Positions held in FEMS Immunology and Medical Microbiology, Biochemical Journal , Current Respiratory Medicine Reviews , South African Journal of Science,Tuberculosis, International Journal of Biochemistry and Cell Biology. Ad hoc reviewer for the following international journals: Cell , Nature Medicine, Nature Reviews Microbiology, PLoS Medicine, PLoS Pathogens, PLoS ONE, Trends in Microbiology, Infection and Immunity, Journal of Bacteriology, Journal of Clinical Microbiology, Applied and Environmental Microbiology, Proceedings of the National Academy of Sciences USA, Journal of Experimental Medicine, Journal of Infectious Diseases, FEMS Microbiology Reviews, Molecular Microbiology, FEMS Microbiology Letters, Expert Opinion on Therapeutic Targets, BMC Microbiology, BMC Infectious Diseases, European Journal of Biochemistry, Microbiology, the Journal of Biological Chemistry, Biochemistry, Gene and Tuberculosis.

RESEARCH GRANTS Pending More Medicines for Tuberculosis (MM4TB), participant in EU FP7 project (Prof. Stewart Cole, PI) (confirmed) 2011-2014 Identification of High-Quality Hits for Tuberculosis (HIT-TB), sub-contractor on Application submitted to Bill & Melinda Gates Foundation; Dr. Clifton Barry, PI) (confirmed) 2011-2013 Current funding Swiss/South Africa Bilateral Research Program (co-PI with Prof. J. D. McKinney) 2009-2011 Seattle Biomedical Research Institute (sub-contractor on grant from Bill & Melinda Gates Foundation) 2007-2010 DST/NRF Centre of Excellence for Biomedical TB Research 2004-2013 HHMI, International Infectious Diseases and Parasitology Program 2000-2010 South African Medical Research Council Research Unit 1 2000-2015 University of the Witwatersrand 2000-2010

Past funding Wellcome Trust Collaborative Research Initiative Grant (co-PI with Prof. N. G. Stoker) 2002-2006 Wellcome Trust Major Equipment grant (#061017, with Prof. K. P. Klugman)) 2000-2003 National Institutes of Health, USA (co-PI with H. Rubin, Univ. of Pennsylvania) 1999-2004 GlaxoSmithKline Action TB Initiative Phase II (UK) 1999-2003 Technology and Human Resource in Industry Programme 1999-2003 National Research Foundation (NRF) Unlocking the Future Programme 2001-2004 National Health Laboratory Service and School of Pathology, Wits University 2002-2004 SAIMR Molecular Biology Unit (Annual research grant) 1989-2001 NRF Open Programme 1996-2000 DACST Lead Programme, Medical Research Council, South Africa 1999-2000 Glaxo Wellcome “ Action TB” Initiative Phase I (UK) 1993-1998 FRD President’s Award Core Programme 1991-1994 Medical Research Council (SA), short-term grants 1991-1993 SmithKline & French (USA) 1989-1990 SA Goldfields Foundation 1989

MEMBERSHIP OF PROFESSIONAL SOCIETIES Current: Fellow, American Academy of Microbiology; Associate Fellow, Academy of Sciences of the Developing World; Fellow, Academy of Science of South Africa; American Association for the Advancement of Science; American Society for Microbiology; Health Professions Council of South Africa (membership #MW008753); Royal Society of South Africa; South African Society for Biochemistry & Molecular Biology.

BIBLIOGRAPHY Articles in peer-reviewed journals

1. Mizrahi, V., and Modro, T.A. 1982. Preparation and fragmentation behavior of dialkylphosphoryl (and phosphinyl) acetyl (and benzoyl) imides and related systems. J. Org. Chem. 47: 3533-3538.

2. Mizrahi, V., and Modro, T.A. 1982. The molecular and crystal structure of N- benzoyldimethylphosphorimidate. Cryst. Struct. Commun. 11: 627-630.

3. Mizrahi, V., Koch, K.R., and Modro, T.A. 1983. N-substituted benzamides. NMR and spectroscopic study on substituent effects. S. Afr. J. Chem. 36:111-113.

4. Mizrahi, V., and Niven, M.L. 1983.The molecular and crystal structures of dibenzimide and its N- methylated analogue: steric and hydrogen-bonding effects. S. Afr. J. Chem. 36:137-142.

5. Mizrahi, V., and Modro, T.A. 1983. Phosphoric-carboxylic imides. II. The neutral and acid-catalyzed solvolysis of phosphoric-carboxylic imides and related systems. J. Org. Chem. 48: 3030-3037.

6. Mizrahi, V., Hendrickse, T.F., and Modro, T.A. 1983. Alkylation of ambident anions derived from phosphoric-caroboxylic imides. N-benzoyl-O,O-dimethyl-O-ethylphosphorimidate and E/Z O-ethyl-N- (dimethylphosphoryl) benzimidate. Can. J. Chem. 61:118-123.

7. Glover, S.A., Mizrahi, V., and Modro, T.A. 1984. MNDO Molecular orbital calculations on N- formylphosphinamide. J. Chem. Soc. Perkin. Trans. II. 325-328.

8. Hendrickse, T.F., Mizrahi, V., and Modro, T.A. 1984. Phosphoric-carboxylic imides IV. The acylation reactions of phosphoric amides and related systems. Phosphorus & Sulfur 20: 93-98.

9. Mizrahi, V., Henrie, R.N., Marlier, J.F., Johnson, K.A., and Benkovic, S.J. 1985. Rate-limiting steps in the DNA polymerase I pathway. Biochemistry 24:4010-4018.

10. Mizrahi, V., Benkovic, P.A., and Benkovic, S.J. 1986. Mechanism of the idling-turnover reaction of the large (Klenow) fragment of DNA polymerase I from Escherichia coli. Proc. Natl. Acad. Sci. USA 83:231- 235.

11. Mizrahi, V., Benkovic, P.A., and Benkovic, S.J. 1986. Mechanism of DNA polymerase I. Exonuclease/ polymerase activity switch and DNA sequence dependence of pyrophosphorolysis and misincorporation reactions. Proc. Natl. Acad. Sci. USA 83:5769-5773.

12. Kuchta, R.D., Mizrahi, V., Benkovic, P.A., Johnson, K.A., and Benkovic, S.J. 1987. Kinetic mechanism of DNA polymerase I (Klenow). Biochemistry 26:8410-8417.

13. Mizrahi, V., and Benkovic, S.J. 1988. The dynamics of DNA polymerase-catalyzed reactions. Adv Enzymol. Relat. Areas Mol. Biol. 61:437-457.

14. Teixeira, A.V.G., Mizrahi, V., and Thomson, J.A. 1988. Investigation into the nature of a Bacillus promoter cloned into a promoter-probe vector. Gene 81: 159-163.

15. Mizrahi, V. 1989. Analysis of the ribonuclease H mechanism of HIV-1 reverse transcriptase using RNA ⋅DNA hybrids derived from the gag region of HIV-1. Biochemistry 28:9088-9094.

16. Mizrahi, V., Lazarus, G.M., Miles, L.M., Meyers, C.A., and Debouck, C. 1989. Recombinant HIV-1 reverse transcriptase: purification, primary structure, and polymerase/ ribonuclease H activities. Arch. Biochem. Biophys. 273:347-358.

17. Dudding, L.R., Harington, A., and Mizrahi, V. 1990. Endoribonucleolytic cleavage of RNA ⋅oligodeoxynucleotide hybrids by the ribonuclease H activity of HIV-1 reverse transcriptase. Biochem. Biophys. Res. Commun. 167: 244-250.

18. Mizrahi, V., Usdin, M.T., Harington, A., and Dudding, L.R. 1990. Site-directed mutagenesis of the conserved Asp443 and Asp498 carboxy-terminal residues of HIV-1 reverse transcriptase. Nucleic Acids Res. 18:5359-5363.

19. Dudding, L.R., Nkabinde, N.C., and Mizrahi, V. 1991. Analysis of the DNA polymerase activities of point mutants of HIV-1 reverse transcriptase lacking ribonuclease H activity. Biochemistry 30:10498-10506.

20. Mizrahi, V., Huberts, P., Dawes, S.S. and Dudding, L.R. 1993. A PCR method for the sequence analysis of gyrA, polA, and rnhA gene segments from mycobacteria. Gene 136: 287-290.

21. Dudding, L.R., and Mizrahi, V. 1993. Rapid kinetic analysis of a point mutant of HIV-1 reverse transcriptase lacking ribonuclease H activity Biochemistry 32: 6116-6120.

22. Mizrahi, V., Brooksbank, R.L. and Nkabinde, N.C. 1994. Site-directed mutagenesis of the conserved Asp443, Glu478, Asn494 and Asp498 residues in the ribonuclease H domain of HIV-1 reverse transcriptase: expression and biochemical analysis. J. Biol. Chem. 269: 19245-19249.

23. Dawes, S.S., Crouch, R.J., Morris, S.L. and Mizrahi, V. 1995. Cloning, sequence analysis, overproduction in Escherichia coli and enzymatic characterization of the RNase HI from Mycobacterium smegmatis. Gene 165: 71-75.

24. Huberts, P., and Mizrahi, V. 1995. Cloning and sequence analysis of the gene encoding the DNA polymerase I from Mycobacterium tuberculosis. Gene 164: 133-136.

25. Gordhan, B.G., Andersen, S.J., De Meyer, A.R. and Mizrahi, V. 1996. Construction by homologous recombination and phenotypic characterization of a DNA polymerase domain polA mutant of Mycobacterium smegmatis. Gene 178: 125-130.

26. Mizrahi, V., and Huberts, P. 1996. Deoxy- and dideoxyribonucleotide discrimination and identification of critical 5’ nuclease domain residues of the DNA polymerase I from Mycobacterium tuberculosis. Nucleic Acids Res. 24:4845-4852.

27. Gordhan, B.G., and Mizrahi, V. 1997. The RLEP-flanked polA gene from Mycobacterium leprae polA gene is not transcribed in Mycobacterium smegmatis. Gene 187:63-66.

28. Durbach, S.I., Andersen, S.J., and Mizrahi, V. 1997. SOS induction in mycobacteria. Analysis of the DNA binding activity of a LexA-like repressor and its role in DNA damage induction of the recA gene from Mycobacterium smegmatis. Mol. Microbiol. 26:643-653.

29. Mizrahi, V. 1997. Genetics and Tuberculosis, Cape Town, South Africa, 21 November 1997. Tubercle and Lung Dis . 78: 171-174.

30. Mizrahi, V. and Andersen, S.J. 1998. DNA repair in Mycobacterium tuberculosis. What have we learnt from the genome sequence? Mol. Microbiol. 29:1331-1339.

31. Boshoff, H.I.M., and Mizrahi, V. 1998. Purification, gene cloning, targeted knockout, overexpression and biochemical characterization of the major pyrazinamidase from Mycobacterium smegmatis. J. Bacteriol. 180: 5809-5814.

32. Downing, K.J., McAdam, R.A., and Mizrahi, V. 1999. Staphylococcus aureus nuclease is a useful secretion reporter for mycobacteria. Gene 239: 293-299.

33. Parish, T., Gordhan, B.G., Quan, S., McAdam, R.A., Duncan, K., Mizrahi, V., and Stoker, N.G. 1999 Production of mutants in amino acid biosynthetic genes of Mycobacterium tuberculosis by homologous recombination. Microbiology 145: 3497-3503.

34. Machowski, E.E., McAdam, R.A., Derbyshire, K.D., and Mizrahi, V. 2000. Construction and application of mycobacterial reporter transposons. Gene 253: 67-75.

35. Boshoff, H.I., and Mizrahi V. 2000. Expression of Mycobacterium smegmatis pyrazinamidas in Mycobacterium tuberculosis confers hypersensitivity to pyrazinamide and related amides. J. Bacteriol . 182: 5479-5485.

36. Primm, T., Andersen, S.J., Mizrahi, V., Avarbock, D., Rubin, H., and Barry, C.E., III. 2000. The stringent response of Mycobacterium tuberculosis is required for long-term survival. J. Bacteriol . 182: 4889- 4898.

37. Boshoff, H.I.M., Durbach, S.I., and Mizrahi, V. 2001. DNA metabolism in Mycobacterium tuberculosis : implications for drug resistance and strain variability. Scand. J. Infect. Dis. 33: 101-105.

38. Dawes, S.S., and Mizrahi, V. 2001. DNA metabolism in Mycobacterium leprae. Lepr. Rev. 72: 408-414.

39. Kana, B.D., Weinstein, E., Dawes, S.S., Rubin, H., and Mizrahi, V. 2001. Characterization of the cydAB- encoded cytochrome bd oxidase from Mycobacterium smegmatis. J. Bacteriol. 183: 7076-7086.

40. Hide, W., Mizrahi, V., Venkatesh, B., Brenner, S., Simpson, A., Blatch, G., Soodyall, H., Denby, K., Wingfield, M., Wingfield, B., van Helden, P., Ramesar, R., Dorrington, R., Kelso, J., Oppon, E., Goyvaerts, E., Ramsay, M., de Villiers, E., van Heerden, C., Allsopp, B., Seoighe, C. 2001. A Platform for genomics in South Africa. S. Afr. Med. J . 91: 1006-1007.

41. Boshoff, H.I., Mizrahi, V., and Barry, C.E., III. 2002. Effects of pyrazinamide on fatty acid synthesis by whole mycobacterial cells and purified fatty acid synthase I . J. Bacteriol. 184: 2167-2172.

42. Gordhan, B.G., Smith, D.A, Alderton, H., McAdam, R.A., Bancroft, G.D., and Mizrahi, V. 2002. Construction and phenotypic characterization of an auxotrophic mutant of Mycobacterium tuberculosis defective in L-arginine biosynthesis. Infect. Immun. 70: 3080-3084.

43. Durbach, S.I., Springer, B., Machowski, E.E., North, R.J., Papavinasasundaram, K.G., Colston, M.J., Böttger, E.C., and Mizrahi, V. 2003. DNA alkylation damage as a sensor if nitrosative stress in Mycobacterium tuberculosis. Infect. Immun . 71: 997-1000.

44. Boshoff, H.I.M., Reed, M.B., Barry, C.E., III, and Mizrahi, V. 2003. DnaE2 polymerase contributes to in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis. Cell 113: 183-193. [Featured article of the issue ].

45. Dahl, J., Kraus, C.N., Doan, B., Foley, K., Avarbock, D., Kaplan, G., Mizrahi V., Rubin, H., and Barry,

C.E. III. 2003. The role of Rel MTb -mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice. Proc. Natl. Acad. Sci. USA . 100: 10026-10031.

46. Dawes, S.S., Warner, D.F., Tsenova, L., Timm, J., McKinney, J.D., Kaplan, G., Rubin, H., and Mizrahi, V. 2003. Ribonucleotide reduction in Mycobacterium tuberculosis: function and expression of the class Ib and class II ribonucleotide reductases. Infect. Immun . 71: 6124-6131.

47. Kana, B.D., and Mizrahi, V. 2004. Molecular genetics of Mycobacterium tuberculosis in relation to the discovery of novel drugs and vaccines. Tuberculosis 84: 63-75.

48. Downing, K.J., Betts, J.C., Young, D.I., Kelly, F., McAdam, R.A., Young, M., and Mizrahi, V. 2004. Global expression profiling of strains harbouring null mutations reveals that the five rpf -like genes of Mycobacterium tuberculosis show functional redundancy. Tuberculosis 84: 167-179.

49. Cowley, S., Ko, M., Pick, N., Chow, R., Downing, K.J., Gordhan, B.G., Betts, J.C., Mizrahi, V., Smith, D.A., Stokes, R.W., and Av-Gay, Y. 2004. The Mycobacterium tuberculosis protein serine/threonine kinase PknG is linked to cellular glutamine/glutamate levels and is important for growth in vivo. Mol. Microbiol . 52: 1691-1702.

50. Warner, D.F. and Mizrahi, V. 2004. Mycobacterial genetics in target validation. Drug Discovery Today: Technologies 1: 93-98.

51. Springer, B., Sander, P., Sedlacek, L., Hardt, W.D., Mizrahi, V., Schär and Böttger, E.C. 2004. Lack of mismatch correction facilitates genome evolution in mycobacteria. Mol. Microbiol . 53: 1601-1609.

52. Boshoff, H.I.M., Barry, C.E. III and Mizrahi, V. 2004. Mutational dynamics in Mycobacterium tuberculosis : Implications for the evolution of drug resistance. S. Afr. J. Sci . 100: 471-474.

53. Machowski, E.E., Dawes, S.S. and Mizrahi, V. 2005. TB tools to tell the tale – molecular genetic methods for mycobacterial research. Int. J. Biochem. Cell Biol . 37: 54-68.

54. Downing, K.J., Mischenko, V.V., Shleeva, M.O., Young, D.I., Young, M., Kaprelyants, A.S., Apt, A.S., and Mizrahi, V. 2005. Mutants of Mycobacterium tuberculosis lacking three of the five rpf -like genes are defective for growth in vivo and for resuscitation in vitro. Infect. Immun . 73: 3038-3043.

55. Kaufmann, S.H.E., Cole, S.T., Mizrahi, V., Rubin E., and Nathan, C. 2005. Mycobacterium tuberculosis and the host response. J. Exp. Med . 201: 1693-1697. (Meeting Review).

56. Matsoso, L.G., Kana, B.D., Crellin, P.K., Lea-Smith, D., Peolis, A., Powell, D.,Dawes, S.S., Rubin, H., Coppel, R.L., and Mizrahi, V. 2005. Function of the cytochrome bc 1-aa 3 branch of the respiratory network in mycobacteria and network adaptation occurring in response to its disruption. J. Bacteriol . 187: 6300-6307.

57. Shi, L., Sohaskey, C.D., Kana, B.D., Dawes, S.S., North, R.J., Mizrahi, V., and Gennaro, M.L. 2005. Changes in energy metabolism in Mycobacterium tuberculosis as revealed by transcriptional profiling during mouse lung infection and under in vitro conditions affecting aerobic respiration. Proc. Natl. Acad. Sci. USA 102: 15629-15634.

58. Cholo, M.C., Boshoff, H.I., Steel, H.C., Cockeran, R., Matlola, N.M. Downing, K.J., Mizrahi, V., and Anderson, R.J. 2006. Effects of clofazimine on potassium uptake by a Trk-deletion mutant of Mycobacterium tuberculosis . J. Antimicrob. Chemother. 57: 79-84.

59. Warner, D.F., Etienne, G., Wang, X.-M., Matsoso, L.G., Soetaert, K., Dawes, S.S., Stoker, N.G., Content, J., and Mizrahi, V. 2006. A derivative of Mycobacterium smegmatis mc 2155 that lacks the duplicated chromosomal region. Tuberculosis 86: 438-444.

60. Gordhan, B.G., Smith, D.A., Kana, B.D., Bancroft, G. and Mizrahi, V. 2006. The carbon-starvation- inducible genes Rv2557 and Rv2558 of Mycobacterium tuberculosis are not required for long-term survival under carbon starvation and for virulence in SCID mice. Tuberculosis 86: 430-437.

61. Warner, D.F. and Mizrahi, V. 2006. Tuberculosis chemotherapy. Influence of stress- and damage- response pathways on drug efficacy. Clin. Microbiol. Rev . 19: 558-570.

62. Machowski, E.E., Barichievy, S., Springer, B., Durbach, S.I., and Mizrahi, V. 2007. In vitro analysis of rates and spectra of mutations in a polymorphic region of the Rv1046 PE_PGRS gene of Mycobacterium tuberculosis . J. Bacteriol . 189: 2190-2195.

63. Warner, D.F., and Mizrahi, V. 2007. The survival kit of Mycobacterium tuberculosis. Nat. Med. 13: 282- 284.

64. Warner, D.F., Savvi, S., Mizrahi, V., and Dawes, S.S. 2007. A riboswitch regulates expression of the coenzyme B12-independent methionine synthase in Mycobacterium tuberculosis : implications for differential methionine synthase function in strains H37Rv and CDC1551. J. Bacterio l. 189: 3655-3659.

65. Kana, B. D., Gordhan, B.G., Downing, K.J., Sung. N., Vostroktunova, G., Machowski, E.E., Tsenova, G., Young, M., Kaprelyants, A., Kaplan, G., and Mizrahi, V. 2008. The resuscitation-promoting factors of Mycobacterium tuberculosis are required for virulence and resuscitation from dormancy but are collectively dispensable for growth in vitro. Mol. Microbiol . 67: 672-684

66. Savvi, S., Warner, D.F., Mizrahi, V., and Dawes, S.S. 2008. Functional characterization of a vitamin B12 - dependent methylmalonyl pathway in Mycobacterium tuberculosis : Implications for propionate metabolism during growth on fatty acids. J. Bacteriol . 190: 3886-3895.

67. Wang, X.M., Galamba, A., Warner, D.F., Soetaert, K., Merkel, J., Kalai, M., Bafani, P., Lefevre, P., Mizrahi, V., and Content, J. 2008. IS 1096 -mediated rearrangements play a key role on genome evolution of Mycobacterium smegmatis . Tuberculosis 88: 399-409

68. Mowa, M.B., Warner, D.F., Kaplan, G., Kana, B.D., and Mizrahi, V. 2009. Function and regulation of class I ribonucleotide reductase-encoding genes. J. Bacteriol . 191: 985-995.

69. Kana, B.D. and Mizrahi, V. 2010. Resuscitation promoting factors as lytic enzymes for bacterial growth and signaling. FEMS Immunol. Med. Microbiol . 58: 39-50.

70. Kana, B.D., Abrahams, G.L., Sung, N., Warner, D.F., Gordhan, B.G., Machowski, E.E. Tsenova, L., Sacchettini, J.C., Stoker, N.G., Kaplan, G., and Mizrahi, V. 2010. Role of the Y-family DNA polymerase- encoding DinB homologs, Rv1537 and Rv3056 in Mycobacterium tuberculosis . J. Bacteriol . 192: 2220- 2227.

71. Ioerger, T., Feng, Y., Ganesula, K., Cheng, X., Dobos, K.M., Fortune, S., Jacobs, W.R., Jr., Mizrahi, V., Parish, T., Rubin, E., Sassetti, C., and Sacchettini, J.C. 2010. Variation among genome sequences of H37Rv strains of M. tuberculosis from multiple laboratories. J . Bacteriol . 192: 3645-3653.

72. Kana, B.D., Mizrahi, V., and Gordhan, B.G. 2010. Depletion of resuscitation promoting factors has limited impact on the drug susceptibility of Mycobacterium tuberculosis . J. Antimicrob. Chemother . 65: 1583-1585.

73. Warner, D.F., Ndwandwe, D.E., Abrahams, G.L., Kana, B.D., Venclovas, Č., and Mizrahi, V. 2010. Essential roles for imuA’ and imuB -encoded accessory factors in DnaE2-dependent mutagenesis in mycobacteria. Proc. Natl. Acad. Sci. USA 107: 13093-13098.

74. Kana, B.D., and Mizrahi, V. 2010. Resuscitation-promoting factors in bacterial population dynamics during TB infection. Drug Discovery Today: Disease Mechanisms 7(1): e13-e18.

75. Williams, M.J., Kana, B.D., and Mizrahi, V. 2011. Functional analysis of molybdopterin biosynthesis in mycobacteria identifies a novel fused molybdopterin synthase in Mycobacterium tuberculosis. J. Bacteriol . 193: 98-106

76. Warner, D.F., and Mizrahi, V. 2011. How Mycobacteria Make Ends Meet. Mol. Microbiol . 79: 283-287 [Invited MicroCommentary]

77. Ahmadou Ahidjo, B., Kuhnert, D., Machoeski, E.E., Gordhan, B.G., Abrahams, G.L., and Mizrahi, V. VapC toxins from Mycobacterium tuberculosis differentially inhibit mycobacterial growth and are specifically neutralized by their cognate VapB antitoxins. Submitted.

Book chapters 78. Thomson, J.A., Harington, A., Prudence, K.W., Teixeira, A.V.G., Mizrahi, V., and Watson, T.G. (1988) Stability and expression in Bacillus subtilis of an α-amylase hybrid plasmid. In: Genetics and Biotechnology of the Bacilli (II) (Ganesan, A.T., and Hoch, J.A., eds.), Academic Press, pp. 331-335.

79. Debouck, C., Deckman, I.C., Lazarus, G.M., and Mizrahi, V. 1990. Expression of active and authentic protease and reverse transcriptase of HIV-1 in bacteria. In: Human Retroviruses (Groopman, J.E., Chen, I., Essex, M., and Weiss, R., eds.), Alan R. Liss, New York, 119: 217-227.

80. Mizrahi, V., Nossal, N.G., and Itaya, M. 1998. Other Enzymes that Degrade the RNA of RNA-DNA Hybrids. In: Ribonucleases H (Crouch, R.J. & Toulmé, J.J., eds.), INSERM Editions (Paris) and John Libbey (London), ch. 8, pp. 168-182.

81. Cazenave, C., Mizrahi, V., and Crouch, R.J. 1998. Methods - rnh and ribonuclease H activity analysis. In: In: Ribonucleases H (Crouch, R.J. & Toulmé J.J., eds.), INSERM Editions (Paris) and John Libbey (London), ch. 12, pp. 251-266.

82. Mizrahi, V., Dawes, S.S., and Rubin, H. 2000. DNA Replication. In: Molecular Genetics of the Mycobacteria (Hatfull, G.F. & Jacobs, W.R., Jr., eds.), ch. 10, pp. 159-172. American Society for Microbiology Press, Washington, D.C.

83. Warner, D.F., and Mizrahi, V. 2003. Mechanisms of stationary-phase mutagenesis in bacteria and their relevance to antibiotic resistance. In : Dormancy and Low-Growth States in Microbial Disease (Coates, A.R., ed.), ch. 5, pp. 131-159. Advances in Molecular and Cellular Microbiology Series, Cambridge University Press, Cambridge, UK.

84. Mizrahi, V., Buckstein, M. and Rubin, H. 2005. Nucleic Acid metabolism. In : Tuberculosis and the Tubercle Bacillus (Cole, S.T., McMurray, D., Gicquel, B., and Jacobs, W.R., Jr, eds), ch. 23, p. 369-378, American Society for Microbiology Press, Washington, D.C.

85. Mizrahi, V. 2007. Mobilising the best brains to deal with poverty and health in Africa: Challenges and opportunities in the world of science. In : Biotechnology and Health: South Africa’s aspirations in health- related biotechnology (J. Chataway & W. James, eds.), Van Schaik Publishers, Pretoria, South Africa, ch. 8, pp. 42-44.

86. Warner, D.F., and Mizrahi, V. 2008. M. tuberculosis physiology. In : Handbook of Tuberculosis: Molecular Biology and Biochemistry (S.H.E. Kaufmann & E.J. Rubin, eds.), WILEY-VCH, Weinheim, Germany, ch. 3, pp. 53-69.

87. Kana, B. D., Machowski, E.E., Schechter, N., Shin, J.-T., Rubin, H., and Mizrahi, V. 2009. Electron transport and respiration. In : Mycobacterium: Genomics and Molecular Biology (Parish, T. & Brown, A., eds.), Horizon Press, London, ch. 3, p. 35-64.

Articles in non-peer-reviewed journals and editorials 88. Kana, B.D., and Mizrahi, V. 2005. Understanding the TB bacterium. Quest: Science for South Africa 1: 7- 9.

89. Mizrahi, V. Key scientific issues pertaining to multi- and extensively drug-resistant tuberculosis. Op-Ed piece, The Cape Times , 8 June 2007.

90. Capron, M., and Mizrahi, V. 2010. Highlights from the first meeting of the Europe-Africa Frontier Research Conference Series. Infectious Diseases: From Basic to Translational Research. FEMS Immunol. Med. Microbiol . 58: 1-2.