MAP1LC3A Antibody Cat. No.: 7579
Western blot analysis of MAP1LC3A in HeLa cell lysate with MAP1LC3A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of MAP1LC3A in human brain tissue Immunohistochemistry of MAP1LC3A in human brain with MAP1LC3A antibody at 20 μg/mL. tissue with MAP1LC3A antibody at 5 μg/mL.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
MAP1LC3A antibody was raised against a 10 amino acid peptide near the amino terminus of human MAP1LC3A. IMMUNOGEN:
The immunogen is located within the first 50 amino acids of MAP1LC3A.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
September 28, 2021 1 https://www.prosci-inc.com/map1lc3a-antibody-7579.html MAP1LC3A antibody can be used for detection of MAP1LC3A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
MAP1LC3A antibody is human specific. At least two isoforms of MAP1LC3A are known to SPECIFICITY: exist. MAP1LC3A antibody is predicted to not cross-react with MAP1LC3B or MAP1LC3C.
POSITIVE CONTROL: 1) Cat. No. 1210 - HEK293 Cell Lysate
2) Cat. No. 1201 - HeLa Cell Lysate
3) Cat. No. 10-301 - Human Brain Tissue Slide
Predicted: 14 kDa PREDICTED MOLECULAR WEIGHT: Observed: 17 kDa
Properties
PURIFICATION: MAP1LC3A antibody is affinity chromatography purified via peptide column.
CLONALITY: Polyclonal
ISOTYPE: IgG
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: MAP1LC3A antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION: 1 mg/mL
MAP1LC3A antibody can be stored at 4˚C for three months and -20˚C, stable for up to STORAGE CONDITIONS: one year.
Additional Info
OFFICIAL SYMBOL: MAP1LC3A
MAP1LC3A Antibody: LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3Autophagy-related protein ALTERNATE NAMES: LC3 A
ACCESSION NO.: NP_852610
PROTEIN GI NO.: 31563518
GENE ID: 84557
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
September 28, 2021 2 https://www.prosci-inc.com/map1lc3a-antibody-7579.html Background and References
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3A belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). BACKGROUND: MAP1LC3A is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3A, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3).
1) Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. REFERENCES: Curr. Opin. Cell Biol. 1995; 7:72-81.
2) Halpain S and Dehmelt L. The MAP1 family of microtubule-associated proteins. Genome Biol. 2006; 7:224.
3) He H, Dang Y, Dai F, et al. Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J. Biol. Chem. 2003; 278:29278-87.
ANTIBODIES FOR RESEARCH USE ONLY.
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September 28, 2021 3 https://www.prosci-inc.com/map1lc3a-antibody-7579.html