EUROPEAN JOURNAL OF IMMUNOLOGY

Supporting Information

for

Eur. J. Immunol. 25381

© Wiley-VCH 2004 69451 Weinheim, Germany Enhanced basal AP-1 activity and de-regulation of numerous genes in T cells transgenic for a dominant interfering mutant of FADD/MORT1 Svetla Chaneva, Günter Schneider, Daniela Siegmund, Harald Wajant, Jörg Mages and Georg Häcker

Legends for Fig. 1 and Tables 1 and 2

Supplementary Fig. 1. Hierarchical analysis of expression profiling. Hierarchical cluster analysis was performed using Genesis Software [31]. The relative expression of each gene in resting and activated (20 h PMA+ionomycin) WT and FADDdn T cells is depicted by color coding (green, low expression; red, high expression). Genes that were differentially expressed by at least a factor of 2 in either resting or activated cells are shown.

[31] Sturn, A., Quackenbush, J. and Trajanoski, Z., Genesis: cluster analysis of microarray data. Bioinformatics 2002. 18: 207–208.

Supplementary Table 1. Genes differentially expressed in WT and FADDdn T cells. Absolute levels and relative changes in the levels of genes differentially expressed between WT and FADDdn T cells. The mRNA levels were determined by oligonucleotide microarray analysis in resting or activated (after 20 h PMA+ionomycin) T cells purified from lymph nodes of WT or FADDdn mice. Shown are only genes whose expression differed by a factor of at least 2 between the two mouse genotypes in either resting or activated T cells. Data were analyzed using the Microarray Suite 5.0 Affymetrix software. A, absent; P, present; M, marginally present; comp, comparison; I, increased; NC, no change; D, decreased. Supplementary Table 2. Expression of genes involved in cell cycle regulation. Absolute levels and relative changes in the levels of cell-cycle-associated genes. The mRNA levels were determined by oligonucleotide microarray analysis in resting or activated (after 20 h PMA+ionomycin) T cells purified from lymph nodes of WT or FADDdn mice. Data were analyzed using the Microarray Suite 5.0 Affymetrix software. A, absent; P, present; M, marginally present; comp, comparison; I, increased; NC, no change; D, decreased. Supplementary figure 1 Supplementary Table 1

Probe set Title Gene Symbol comp 0h Change comp 20h Change 0h Fold Change 20h Fold Change 0h WT Signal 0h WT Detection Signal 0h FADD 0h FADD Detection 20h WT Signal 20h WT Detection Signal 20h FADD Detection 20h FADD AFFX-MurIL2_ Interleukin 2 Il2 10.1 A 10 A 121 P 704 P NC I 1.0 5.8 AFFX-18SRNA------1122 P 3903 P 1759 P 307 P I D 3.5 0.2 AFFX-18SRNA------4496 P 6653 P 1996 P 494 P I D 1.5 0.2 99982_at nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, beta Nfkbib 91.8 A 330 M 601 P 802 P I I 3.6 1.3 99871_f_at ------31.8 A 142 A 38.1 A 93.5 M NC I 4.4 2.5 99665_at special AT-rich sequence binding protein 1 Satb1 3146 P 2717 P 668 P 1368 P D I 0.9 2.0 99392_at tumor necrosis factor, alpha-induced protein 3 Tnfaip3 255 P 369 P 138 P 315 P I I 1.4 2.3 99323_at interleukin 12 receptor, beta 2 Il12rb2 5.5 A 7.8 A 26.6 A 100 P NC I 1.4 3.8 99126_at inactive X specific transcripts Xist 654 P 517 P 335 P 1.5 A NC D 0.8 0.0 99051_at S100 calcium binding protein A4 S100a4 185 P 336 P 18.9 A 34.8 A I NC 1.8 1.8 99023_at platelet-activating factor acetylhydrolase, isoform 1b, alpha2 subunit Pafah1b2 43.1 A 104 P 123 P 151 P I I 2.4 1.2 98868_at B-cell leukemia/lymphoma 2 Bcl2 366 P 334 P 485 P 263 P NC D 0.9 0.5 98600_at S100 calcium binding protein A11 (calizzarin) S100a11 733 P 691 P 845 P 360 P NC D 0.9 0.4 98596_s_at sialyltransferase 9 (CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransfera Siat9 25.2 A 22.9 A 93 M 37.4 A NC D 0.9 0.4 98525_f_at erythroid differentiation regulator edr 6730 P 4861 P 6059 P 2808 P D D 0.7 0.5 98406_at chemokine (C-C motif) ligand 5 Ccl5 616 P 1881 P 17.6 A 159 P I I 3.1 9.0 98405_at serine (or cysteine) proteinase inhibitor, clade B, member 9 Serpinb9 88.4 M 82.1 P 174 P 1278 P NC I 0.9 7.3 98392_at lymphocyte-activation gene 3 Lag3 67.6 A 39.2 A 72.9 A 159 P NC I 0.6 2.2 98282_at inducible T-cell co-stimulator Icos 89.1 A 127 P 525 P 268 P I D 1.4 0.5 98026_g_at ecotropic viral integration site 2 Evi2 113 P 151 P 61.4 P 8.5 A NC D 1.3 0.1 98025_at ecotropic viral integration site 2 Evi2 118 P 126 P 61.7 P 6.1 A NC D 1.1 0.1 98002_at interferon consensus sequence binding protein 1 Icsbp1 189 P 228 P 443 P 844 P NC I 1.2 1.9 97911_at Mus musculus cDNA clone IMAGE:5683426, partial cds --- 370 P 325 P 341 P 156 P NC D 0.9 0.5 97844_at regulator of G-protein signaling 2 Rgs2 239 P 91.4 P 27.8 P 124 P D I 0.4 4.4 97718_at cytotoxic T-lymphocyte-associated protein 4 Ctla4 62.5 P 157 P 192 P 249 P I NC 2.5 1.3 97560_at ------94 M 98 P 97.3 P 36.4 P NC D 1.0 0.4 97409_at interferon inducible protein 1 Ifi1 87.5 P 97.6 P 33.7 P 129 P NC I 1.1 3.8 97106_at mitogen activated protein kinase kinase kinase 8 Map3k8 9.9 A 43.4 A 5.7 A 116 P NC I 4.4 20.3 96795_at fatty acid amide hydrolase Faah 341 P 292 P 374 P 163 P NC D 0.9 0.4 96764_at interferon-inducible GTPase Iigp-pending 187 M 379 P 47.3 A 403 P I I 2.0 8.5 96752_at intercellular adhesion molecule Icam1 75.7 P 132 P 79.9 P 230 P NC I 1.7 2.9 96574_at interleukin 9 Il9 28.2 A 57.6 A 64.3 A 648 P NC I 2.0 10.1 96553_at G-protein coupled receptor 25 Gpcr25 156 P 164 P 79.8 P 259 P NC I 1.1 3.2 96486_at Mus musculus 3 days neonate thymus cDNA, RIKEN full-length enriched li --- 244 P 299 P 181 P 61.7 P NC D 1.2 0.3 96254_at DnaJ (Hsp40) homolog, subfamily B, member 1 Dnajb1 608 P 318 P 237 P 298 P D NC 0.5 1.3 96215_f_at Mus musculus, clone IMAGE:3983821, mRNA, partial cds --- 2751 P 1317 P 2082 P 1430 P D D 0.5 0.7 96183_at forkhead box P1 Foxp1 611 P 625 P 1049 P 472 P NC D 1.0 0.4 95731_at Mus musculus Sestrin 1, mRNA (cDNA clone MGC:67135 IMAGE:641452 --- 387 P 295 P 254 P 110 P NC D 0.8 0.4 95721_at MAP kinase-activated protein kinase 2 Mapkapk2 277 P 467 P 581 P 1409 P I I 1.7 2.4 95706_at lectin, galactose binding, soluble 3 Lgals3 4.4 A 62.3 P 14.6 A 20.8 A I NC 14.2 1.4 95655_at RIKEN cDNA 5830411E10 gene 5830411E10R 31.3 P 29.6 P 41.9 P 94.3 P NC I 0.9 2.3 95508_at NCK-associated protein 1 Nckap1 46 A 43.4 A 72.7 P 170 P NC I 0.9 2.3 95466_at coactosin-like 1 (Dictyostelium) Cotl1 233 P 285 P 188 P 87.2 P NC D 1.2 0.5 95095_at flotillin 1 Flot1 283 A 303 A 144 A 277 P NC I 1.1 1.9 94928_at tumor necrosis factor receptor superfamily, member 1b Tnfrsf1b 214 P 219 P 375 P 158 P NC D 1.0 0.4 94881_at cyclin-dependent kinase inhibitor 1A (P21) Cdkn1a 27.5 A 9.1 A 87.1 P 197 P NC I 0.3 2.3 94781_at hemoglobin alpha, adult chain 1 Hba-a1 1316 P 815 P 60.2 A 222 P D I 0.6 3.7 94516_f_at preproenkephalin 1 Penk1 68 P 162 P 53.2 P 240 P I I 2.4 4.5 94458_at caspase 6 Casp6 34.8 A 7.5 A 40.6 P 129 P NC I 0.2 3.2 94432_at sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase) Siat1 419 P 338 P 516 P 130 P D D 0.8 0.3 94387_at spermatogenesis associated 5 Spata5 28.5 A 43.4 P 91.1 P 292 P NC I 1.5 3.2 94256_at DNA segment, Jeremy M. Boss 3 D0Jmb3 112 P 200 P 268 P 999 P I I 1.8 3.7 94255_g_at chloride intracellular channel 4 (mitochondrial) Clic4 65.5 P 83.6 P 119 P 427 P NC I 1.3 3.6 94186_at Tnf receptor-associated factor 1 Traf1 359 P 393 P 264 P 505 P NC I 1.1 1.9 94146_at chemokine (C-C motif) ligand 4 Ccl4 16.1 A 86.3 A 51.8 A 170 P NC I 5.4 3.3 94085_at proteoglycan, secretory granule Prg 178 P 219 P 110 P 442 P NC I 1.2 4.0 93953_at protease, serine, 12 neurotrypsin (motopsin) Prss12 58.5 A 44 A 86 P 23.9 A NC D 0.8 0.3 93875_at heat shock protein 1A Hspa1a 5910 P 567 P 16 A 71.2 P D I 0.1 4.5 93804_at solute carrier family 2 (facilitated glucose transporter), member 3 Slc2a3 81 P 191 P 434 P 144 P I D 2.4 0.3 93677_at killer cell lectin-like receptor, subfamily D, member 1 Klrd1 614 P 431 P 112 P 35.5 A D D 0.7 0.3 93584_at immunoglobulin heavy chain 6 (heavy chain of IgM) Igh-6 3263 P 2464 P 552 P 252 P D D 0.8 0.5 93465_at Jun dimerization protein p21SNFT Snft-pending 87.4 P 79.6 P 55.8 P 232 P NC I 0.9 4.2 93285_at dual specificity phosphatase 6 Dusp6 133 P 167 P 388 P 184 P NC D 1.3 0.5 93116_at protein kinase, cAMP dependent, catalytic, beta Prkacb 1113 P 869 P 399 P 297 P D D 0.8 0.7 93086_at immunoglobulin kappa chain variable 8 (V8) Igk-V8 9459 P 8268 P 260 P 2046 P NC I 0.9 7.9 93078_at lymphocyte antigen 6 complex, locus A Ly6a 1540 P 4061 P 929 P 5211 P I I 2.6 5.6 92901_at retinoic acid receptor, alpha Rara 22.1 A 15 A 110 P 36.7 A NC D 0.7 0.3 92873_f_at protease, serine, 2 Prss2 8.2 A 28.8 A 40.2 A 107 P NC I 3.5 2.7 92845_at 3-oxoacid CoA transferase Oxct 204 P 185 P 361 P 170 P NC D 0.9 0.5 92614_at inhibitor of DNA binding 3 Idb3 337 P 350 A 574 P 233 P NC D 1.0 0.4 92539_at S100 calcium binding protein A10 (calpactin) S100a10 1828 P 2106 P 2706 P 1563 P NC D 1.2 0.6 92305_s_at POU domain, class 2, transcription factor 2 Pou2f2 106 A 39.6 A 454 P 141 P NC D 0.4 0.3 92292_at solute carrier family 2 (facilitated glucose transporter), member 3 Slc2a3 93.6 A 45.5 A 247 P 107 P NC D 0.5 0.4 92280_at actinin, alpha 1 Actn1 85.6 P 91.9 P 123 P 36.9 M NC D 1.1 0.3 92249_g_at nuclear receptor subfamily 4, group A, member 2 Nr4a2 21.2 A 69.9 P 12.3 A 71 P NC I 3.3 5.8 92248_at nuclear receptor subfamily 4, group A, member 2 Nr4a2 37.4 A 55.8 A 11.3 A 88.1 P NC I 1.5 7.8 92232_at suppressor of cytokine signaling 3 Socs3 191 P 603 P 57.3 A 455 P I I 3.2 7.9 162206_f_at suppressor of cytokine signaling 3 Socs3 260 P 506 P 43.1 P 251 P I I 1.9 5.8 161968_f_at chemokine (C-C motif) receptor 5 Ccr5 1.3 A 42.7 M 6.2 A 102 P I I 32.8 16.4 161914_s_at lymphocyte specific 1 Lsp1 3006 P 2749 P 1336 P 602 P NC D 0.9 0.5 161817_f_at RIKEN cDNA 4930422J18 gene 4930422J18R 149 P 105 P 42.3 P 96.3 P NC I 0.7 2.3 161689_f_at interleukin 1 receptor, type II Il1r2 23.5 A 88.6 A 93.2 P 265 P NC I 3.8 2.8 161684_r_at lipocalin 2 Lcn2 70.2 M 66.6 P 68 A 122 P NC I 0.9 1.8 161666_f_at growth arrest and DNA-damage-inducible 45 beta Gadd45b 134 P 211 P 24.6 P 270 P I I 1.6 11.0 160901_at FBJ osteosarcoma oncogene Fos 3384 P 3186 P 14.2 A 95.1 P NC I 0.9 6.7 160829_at pleckstrin homology-like domain, family A, member 1 Phlda1 84.8 P 29.4 M 39.6 P 14.4 A D D 0.3 0.4 160601_at lunatic fringe gene homolog (Drosophila) Lfng 58.3 A 68.1 A 178 P 58.7 A NC D 1.2 0.3 160541_at eukaryotic translation initiation factor 3, subunit 5 (epsilon) Eif3s5 81.9 A 59.7 A 23 A 77.8 M NC I 0.7 3.4 160473_at expressed in non-metastatic cells 4, protein Nme4 59.4 A 45.2 A 180 P 98.9 P NC D 0.8 0.5 160353_i_at MAP kinase-activated protein kinase 2 Mapkapk2 26.9 A 67.6 A 106 P 227 P NC I 2.5 2.1 104728_at protein S (alpha) Pros1 21.9 A 5.4 A 9.8 A 144 P NC I 0.2 14.7 104701_at basic helix-loop-helix domain containing, class B2 Bhlhb2 35.9 A 53 A 194 P 437 P NC I 1.5 2.3 104692_at selectin, platelet Selp 8.8 A 7.6 A 60.2 A 402 P NC I 0.9 6.7 104597_at guanylate nucleotide binding protein 2 Gbp2 459 P 646 P 1184 P 3968 P I I 1.4 3.4 104418_at DNA segment, Chr 6, ERATO Doi 365, expressed D6Ertd365e 183 P 162 P 96.5 P 36.1 P NC D 0.9 0.4 104396_at RIKEN cDNA 2610209C05 gene 2610209C05R 601 P 533 P 309 P 160 P NC D 0.9 0.5 104155_f_at activating transcription factor 3 Atf3 61.4 P 69.8 A 146 P 423 P NC I 1.1 2.9 104149_at nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alphaNfkbia 565 P 866 P 253 P 490 P I I 1.5 1.9 104093_at lymphocyte specific 1 Lsp1 2068 P 1997 P 1369 P 570 P NC D 1.0 0.4 103990_at FBJ osteosarcoma oncogene B Fosb 388 P 807 P 55.5 A 129 P I I 2.1 2.3 103963_f_at interferon-inducible GTPase Iigp-pending 74.8 P 264 P 42.9 P 231 P I I 3.5 5.4 103891_i_at eleven-nineteen lysine-rich leukemia gene 2 Ell2-pending 4.4 A 3 A 53.1 A 107 P NC I 0.7 2.0 103842_at DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked Ddx3y 53.5 P 93.1 P 62.4 P 139 P NC I 1.7 2.2 103833_at homeodomain interacting protein kinase 2 Hipk2 18.9 A 95.5 A 54.6 A 296 P NC I 5.1 5.4 103674_f_at eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked Eif2s3y 52 A 72.1 P 29.5 P 86.7 P I I 1.4 2.9 103615_at expressed sequence AI447904 AI447904 138 P 83 P 91.9 P 21.8 P NC D 0.6 0.2 103563_at Mus musculus 10 days neonate olfactory brain cDNA, RIKEN full-length en--- 60.7 A 151 A 278 P 1176 P NC I 2.5 4.2 103562_f_at ------300 P 637 P 165 P 127 P I NC 2.1 0.8 103443_at absent in melanoma 1 Aim1 458 P 396 P 244 P 476 P NC I 0.9 1.9 103303_at Mus musculus transcribed sequences --- 136 P 97.1 P 53.4 P 107 P NC I 0.7 2.0 103259_at growth factor independent 1 Gfi1 61.3 P 84.3 P 19.7 A 236 P NC I 1.4 12.0 103202_at guanylate nucleotide binding protein 3 Gbp3 99.3 P 219 P 49 P 206 P I I 2.2 4.2 103200_at RIKEN cDNA 6330500D04 gene 6330500D04R 304 P 267 P 136 P 51 P NC D 0.9 0.4 103091_at avian reticuloendotheliosis viral (v-rel) oncogene related B Relb 154 A 263 P 375 P 465 P I I 1.7 1.2 102975_at CD8 antigen, alpha chain Cd8a 1705 P 1415 P 462 P 210 P D D 0.8 0.5 102962_at septin 1 Sept1 481 P 524 P 274 P 125 P NC D 1.1 0.5 102906_at T-cell specific GTPase Tgtp 2008 P 1666 P 233 P 547 P NC I 0.8 2.3 102905_at caspase 4, apoptosis-related cysteine protease Casp4 53.9 A 118 P 11 A 67.3 P I I 2.2 6.1 102877_at granzyme B Gzmb 44.5 A 84.4 P 330 P 509 P I I 1.9 1.5 102860_at serine (or cysteine) proteinase inhibitor, clade A, member 3G Serpina3g 211 A 602 A 129 A 421 P I I 2.9 3.3 102839_at phospholipid scramblase 1 Plscr1 7.6 A 26.9 A 191 P 518 P NC I 3.5 2.7 102779_at growth arrest and DNA-damage-inducible 45 beta Gadd45b 187 A 211 P 33.5 A 127 P NC I 1.1 3.8 102661_at early growth response 2 Egr2 189 P 141 P 179 P 487 P NC I 0.7 2.7 102658_at interleukin 1 receptor, type II Il1r2 70.4 A 89 P 92.7 P 222 P NC I 1.3 2.4 102650_at regulator of G-protein signaling 11 Rgs11 27.2 A 20.9 A 145 P 75.7 A NC D 0.8 0.5 102644_at kidney cell line derived transcript 1 Kdt1 48.7 P 70.4 P 85.7 P 346 P NC I 1.4 4.0 102424_at chemokine (C-C motif) ligand 3 Ccl3 50.4 A 78.3 A 41.5 A 139 P NC I 1.6 3.3 102371_at nuclear receptor subfamily 4, group A, member 1 Nr4a1 136 P 237 P 93.9 P 204 P NC I 1.7 2.2 102362_i_at Jun-B oncogene Junb 957 P 1614 P 493 P 848 P I I 1.7 1.7 102264_at schlafen 1 Slfn1 456 P 396 P 278 P 72.7 P NC D 0.9 0.3 102239_at B-cell leukemia/lymphoma 3 Bcl3 214 A 626 P 138 A 729 P I I 2.9 5.3 102156_f_at ------1825 P 1884 P 44.8 P 314 P NC I 1.0 7.0 102125_f_at ------36.5 A 60.6 P 27.7 P 102 P NC I 1.7 3.7 102064_at caspase 1 Casp1 64.3 P 142 P 9.8 A 18.8 P I I 2.2 1.9 101979_at growth arrest and DNA-damage-inducible 45 gamma Gadd45g 74.4 A 101 A 36.5 A 201 P NC I 1.4 5.5 101926_at proviral integration site 2 Pim2 306 P 296 P 2968 P 1258 P NC D 1.0 0.4 101869_s_at hemoglobin, beta adult minor chain Hbb-b2 1177 P 736 P 40.2 A 153 P D I 0.6 3.8 101857_at serine/arginine-rich protein specific kinase 2 Srpk2 52 P 52.6 M 63.5 P 12.2 A NC D 1.0 0.2 101851_at antigen identified by monoclonal antibody MRC OX-2 Mox2 37.1 A 46.1 M 111 P 244 P NC I 1.2 2.2 101835_at lymphoid-restricted membrane protein Lrmp 297 P 355 P 308 P 147 P NC D 1.2 0.5 101753_s_at P lysozyme structural Lzp-s 6.5 A 326 P 1.7 A 3.1 A I NC 50.2 1.8 101727_at nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor,Nfkbie 166 A 57.8 A 67.6 A 137 P NC I 0.3 2.0 101571_g_at insulin-like growth factor binding protein 4 Igfbp4 714 P 1321 P 730 P 497 P I D 1.8 0.7 101457_at Janus kinase 2 Jak2 161 P 146 P 193 P 410 P NC I 0.9 2.1 101054_at Ia-associated invariant chain Ii 3914 P 4536 P 223 P 475 P NC I 1.2 2.1 100946_at ------7059 P 887 P 14.3 A 40.7 P D I 0.1 2.8 100902_at RIKEN cDNA 2610019F03 gene 2610019F03R 510 P 458 P 194 P 100 P D D 0.9 0.5 100611_at lysozyme Lyzs 15 A 174 P 1.2 A 5.8 A I NC 11.6 4.8 100583_at immunoglobulin heavy chain (J558 family) Igh-VJ558 108 A 235 P 44.1 A 139 P I I 2.2 3.1 100569_at annexin A2 Anxa2 124 A 264 P 617 P 308 P I D 2.1 0.5 100533_s_at cAMP responsive element modulator Crem 37.3 A 53.7 P 79 P 193 P NC I 1.4 2.4 100484_at matrix metalloproteinase 13 Mmp13 19.2 A 7.7 A 45 P 165 P NC I 0.4 3.7 100423_f_at signal transducer and activator of transcription 5A Stat5a 539 P 528 P 498 P 1334 P NC I 1.0 2.7 100422_i_at signal transducer and activator of transcription 5A Stat5a 982 P 967 P 839 P 1709 P NC I 1.0 2.0 100418_at guanine nucleotide binding protein (G protein), gamma 2 subunit Gng2 53.2 A 79.5 P 158 P 66.3 P NC D 1.5 0.4 100397_at TYRO protein tyrosine kinase binding protein Tyrobp 60.4 A 205 P 9.9 A 23.9 A I NC 3.4 2.4 100387_f_at killer cell lectin-like receptor subfamily A, member 13 Klra13 90.9 P 21 A 18.5 P 3.1 A D D 0.2 0.2 100325_at glycoprotein 49 A Gp49a 3.7 A 36.4 P 9.3 A 120 P I I 9.8 12.9 100001_at CD3 antigen, gamma polypeptide Cd3g 2340 P 2456 P 866 P 406 P NC D 1.0 0.5 Supplementary Table 2

Gene Probe set Title Symbol 0h WT Signal 0h WT Detection Signal 0h FADD 0h FADD Detection 20h WT Signal 20h WT Detection Signal 20h FADD Detection 20h FADD comp 0h Change comp 20h Change 0h Fold Change Fold Change20h 99558_at cyclin C Ccnc 58.4 M 94.2 P 109 P 128 P NC NC 1.6 1.2 99186_at cyclin A2 Ccna2 161 A 190 A 155 A 205 M NC I 1.2 1.3 99073_at cyclin F Ccnf 99.3 P 153 P 149 P 174 P NC NC 1.5 1.2 98478_at cyclin G2 Ccng2 221 P 223 P 235 P 172 P NC D 1.0 0.7 97504_at cyclin D2 Ccnd2 1830 P 1868 P 916 P 836 P NC NC 1.0 0.9 97182_at cyclin E2 Ccne2 8.1 A 12.7 A 6 A 10.3 P NC I 1.6 1.7 94820_r_at cyclin I Ccni 47.4 P 29.3 P 9.3 P 14.3 P NC NC 0.6 1.5 94819_f_at cyclin I Ccni 655 P 570 P 478 P 314 P NC D 0.9 0.7 94294_at cyclin B2 Ccnb2 182 P 262 P 147 P 166 P NC NC 1.4 1.1 94272_g_at cyclin D binding myb-like transcription factor 1 Dmtf1 173 A 200 A 45.9 A 87.4 A NC I 1.2 1.9 94271_at cyclin D binding myb-like transcription factor 1 Dmtf1 29.6 A 2.4 A 0.8 A 8.3 A D NC 0.1 10.4 94232_at cyclin D1 Ccnd1 57 A 65.2 A 3 A 19.3 A NC NC 1.1 6.4 94231_at cyclin D1 Ccnd1 123 A 111 A 12.5 A 36.8 A NC NC 0.9 2.9 93712_at cyclin T1 Ccnt1 193 P 199 P 237 P 168 P NC NC 1.0 0.7 92911_at cyclin A1 Ccna1 7.3 A 5.3 A 4.3 A 4.3 A NC I 0.7 1.0 161527_r_at cyclin I Ccni 4.7 A 0.6 A 1.1 A 4.7 A NC NC 0.1 4.3 160545_at cyclin D3 Ccnd3 1002 P 944 P 753 P 728 P NC NC 0.9 1.0 160159_at cyclin B1 Ccnb1 2.7 A 4.8 A 38.2 P 49 P NC NC 1.8 1.3 160127_at cyclin G1 Ccng1 95.9 P 69.9 P 67.3 P 90.7 P NC NC 0.7 1.3 103034_at cyclin E1 Ccne1 263 A 205 A 266 A 257 A NC NC 0.8 1.0 102781_at cyclin L2 Ccnl2 725 P 543 P 322 P 367 P NC NC 0.7 1.1 98789_at cyclin-dependent kinase inhibitor 2A Cdkn2a 54.7 A 69.2 A 5.7 A 24.8 A NC NC 1.3 4.4 98532_at CDK2 (cyclin-dependent kinase 2)-associated protein 1 Cdkap1 264 P 319 P 1182 P 1212 P NC NC 1.2 1.0 98067_at cyclin-dependent kinase inhibitor 1A (P21) Cdkn1a 92.1 A 109 A 40.8 A 130 A NC I 1.2 3.2 96726_at cyclin-dependent kinase 8 Cdk8 208 P 189 P 349 P 363 P NC NC 0.9 1.0 95471_at cyclin-dependent kinase inhibitor 1C (P57) Cdkn1c 28 A 5.2 A 15.3 A 20.1 A D NC 0.2 1.3 95037_at cyclin-dependent kinase 9 (CDC2-related kinase) Cdk9 319 P 301 P 354 P 313 P NC NC 0.9 0.9 94881_at cyclin-dependent kinase inhibitor 1A (P21) Cdkn1a 27.5 A 9.1 A 87.1 P 197 P NC I 0.3 2.3 94521_at cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) Cdkn2d 684 P 656 P 255 P 323 P NC NC 1.0 1.3 94412_at cyclin-dependent kinase 2 Cdk2 126 P 103 P 272 P 247 P NC NC 0.8 0.9 94411_at cyclin-dependent kinase 2 Cdk2 38.9 A 8.8 A 8.6 A 19.1 A NC NC 0.2 2.2 161010_r_at cyclin-dependent kinase inhibitor 1B (P27) Cdkn1b 39.8 A 36.8 P 10.3 A 12 A NC NC 0.9 1.2 160658_at cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase) Cdk7 56.9 A 49.2 A 51.7 M 70.7 P NC NC 0.9 1.4 160638_at cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4) Cdkn2c 117 M 165 P 95.9 P 75.8 A NC NC 1.4 0.8 160623_at cyclin-dependent kinase-like 2 (CDC2-related kinase) Cdkl2 7 A 21.2 A 3.1 A 8.4 A NC NC 3.0 2.7 160538_at cyclin-dependent kinase 4 Cdk4 617 P 636 P 1826 P 1856 P NC NC 1.0 1.0 102664_at cyclin-dependent kinase 5, regulatory subunit (p35) Cdk5r 40.6 A 58 A 24.4 A 17.3 A NC NC 1.4 0.7 101900_at cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) Cdkn2b 4.5 A 5.5 A 1 A 3 A NC NC 1.2 3.0 101483_at cyclin D-type binding-protein 1 Ccndbp1 546 P 638 P 336 P 386 P NC NC 1.2 1.1 101335_at cyclin-dependent kinase 5, regulatory subunit 2 (p39) Cdk5r2 8.7 A 64.7 A 5.9 A 20.8 A NC NC 7.4 3.5 101017_at cyclin-dependent kinase 4 Cdk4 153 P 112 P 136 P 202 P NC NC 0.7 1.5 100444_at cyclin-dependent kinase 5 Cdk5 127 A 121 A 151 P 123 P NC D 1.0 0.8 100278_at cyclin-dependent kinase inhibitor 1B (P27) Cdkn1b 81.6 A 125 A 69.4 A 44.5 A I NC 1.5 0.6