DPAGT1 Antibody Cat. No.: 6485

DPAGT1 Antibody

Immunofluorescence of DPAGT1 in kidney tissue Immunohistochemistry of DPAGT1 in human kidney tissue with DPAGT1 antibody at 20 μg/mL. with DPAGT1 antibody at 2.5 μg/mL.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse

HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (88%)

DPAGT1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human DPAGT1. IMMUNOGEN:

The immunogen is located within amino acids 30 - 80 of DPAGT1.

TESTED APPLICATIONS: ELISA, IF, IHC-P, WB

September 29, 2021 1 https://www.prosci-inc.com/dpagt1-antibody-6485.html DPAGT1 antibody can be used for detection of DPAGT1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

At least four isoforms of DPAGT1 are known to exist; this antibody will recognize the two SPECIFICITY: longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1.

POSITIVE CONTROL: 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate

2) Cat. No. 10-401 - Human Kidney Tissue Slide

Properties

PURIFICATION: DPAGT1 Antibody is affinity chromatography purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: DPAGT1 Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION: 1 mg/mL

DPAGT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: DPAGT1

DPAGT1 Antibody: GPT, ALG7, DGPT, G1PT, UAGT, UGAT, CDG1J, DPAGT, CDG-Ij, CMSTA2, ALTERNATE NAMES: DPAGT2, D11S366, UDP-N-acetylglucosamine--dolichyl-phosphate N- acetylglucosaminephosphotransferase, GlcNAc-1-P transferase

ACCESSION NO.: NP_001373

PROTEIN GI NO.: 42794009

GENE ID: 1798

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 29, 2021 2 https://www.prosci-inc.com/dpagt1-antibody-6485.html DPAGT1 Antibody: The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl- glucosaminephosphotransferase (DPAGT1) is an that catalyzes the first step in the -linked oligosaccharide pathway for . in this integral endoplasmic reticulum (ER) membrane enzyme belongs to the BACKGROUND: family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.

1) Wu X, Rush JS, Karaoglu D, et al. Deficiency of UDP-GlcNAc:Dolichol Phosphate N- REFERENCES: Acetylglucosamine-1 Phosphate Transferase (DPAGT1) causes a novel congenital disorder of glycosylation type Ij. Hum. Mutat. 2003; 22:144-50.

2) Bretthauer RK. Structure, expression, and regulation of UDP-GlcNAc:dolichol phosphate GlcNAc-1-phosphate transferase (DPAGT1). Curr. Drug Targets 2009; 10:477-82

3) Sengupta PK, Bouchie MP, and Kukuruzinska MA. N-glycosylation DPAGT1 is a target of the Wnt/beta-catenin signaling pathway. J. Biol. Chem. 2010; 285:31164-73.

ANTIBODIES FOR RESEARCH USE ONLY.

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