Sarcoma

FUSIONPlex Archer™ FusionPlex™ Sarcoma Panel Genomic translocations represent an important aspect in sarcoma biology. Translocations have been reported in 20-30% of soft tissue cancers, with more than Assay Targets 30 distinct fusions recurring in specific sarcoma Includes the following and their fusion partners: subtypes. Traditional methods of detecting transloca- ALK FUS NTRK3 TCF12 tions, such as FISH, lack the high-resolution molecular CAMTA1 GLI1 PDGFB TFE3 characterization crucial for understanding the identity CCNB3 HMGA2 PLAG1 TFG of these fusion partners. Amplicon-based enrichment methods (such as RT-PCR or traditional targeted CIC JAZF1 ROS1 USP6 sequencing) enable fusion identification, but require that EPC1 MEAF6 SS18 YWHAE fusion partners and breakpoints be known in advance. EWSR1 MKL2 STAT6 The FusionPlex Sarcoma Panel is a targeted FOXO1 NCOA2 TAF15 sequencing assay that simultaneously detects and identifies fusions of 26 genes associated with soft tissue cancers. Sarcoma Fusion Map

Using Archer’s proprietary Anchored Multiplex PCR™ based 5’ ROS1 P tner artner Par enrichment, fusions of all genes in this panel can be identified 3’ ALK

WWTR1 USP6 YWHAE EWSR1 in a single sequencing assay, even without prior knowledge NCOA2 NR4A3 FUS of fusion partners or breakpoints. SS18 PHF1 COL1A1 FKHR JAZF1 PDGFB TPM3 TPM4 TIC ERG A CARS DD1T3 CLTC RANBP2 A • Innovative – first comprehensive TF1 SEC31A GLI1 CD74 CCNB3 EZR NGS-based test on the market MKL2 GOPC DUX4 LRIG3 SDC4 NTRK3 • Comprehensive – identify sarcoma- SLC34A2 AT6 ST CDH11 YAP1 CNBP related fusions in a single assay JJAZ1 MYH9 PLAG1 OMD SSX4 THRAP3 • Streamlined – reduce turn-around SSX2 AHRR HEY1 SSX1 SRF TEAD time and eliminate reflex testing CREB3L2 TFE3 ZSG CREB3L1 NAB2 LPP ZNF444WT1 • Detailed – characterize all molecular HMGA2 ETV6 PBX1 COL1A2 POU5F1 TC2 CIC A C1 FLI1 BCOR ASPSCR1 FEV ACTB NF MEAF6 EPC 1orf95 P

fusion partners ETV1 AX7 E1AF

TFG

AX3 CREB1 AF15

P

TCF12 T

AM22A-B CAMTA1 F For Research Use Only. Not for use in diagnostic procedures. The FusionPlex Sarcoma Panel can be used to identify all of the genes list- ed in the diagram above. By targeting the genes highlighted in red, our Learn more about the FusionPlex Sarcoma Panel at Anchored Multiplex PCR enrichment chemistry has the ability to identify each of www.archerdx.com/sarcoma these sarcoma-associated fusion pairs. PN-MKT-0016 REV A