Zootaxa 4272 (4): 529–550 ISSN 1175-5326 (print edition) http://www.mapress.com/j/zt/ Article ZOOTAXA Copyright © 2017 Magnolia Press ISSN 1175-5334 (online edition) https://doi.org/10.11646/zootaxa.4272.4.3 http://zoobank.org/urn:lsid:zoobank.org:pub:C6234E29-8808-44DF-AD15-07E82B398D66 A new Neotibicen cicada subspecies (Hemiptera: Cicadidae) from the southeast- ern USA forms hybrid zones with a widespread relative despite a divergent male calling song DAVID C. MARSHALL1 & KATHY B. R. HILL Dept. of Ecology and Evolutionary Biology, University of Connecticut, 75 N. Eagleville Rd., Storrs, CT 06269 USA 1Corresponding author. E-mail:
[email protected] Abstract A morphologically cryptic subspecies of Neotibicen similaris (Smith and Grossbeck) is described from forests of the Apalachicola region of the southeastern United States. Although the new form exhibits a highly distinctive male calling song, it hybridizes extensively where it meets populations of the nominate subspecies in parapatry, by which it is nearly surrounded. This is the first reported example of hybridization between North American nonperiodical cicadas. Acoustic and morphological characters are added to the original description of the nominate subspecies, and illustrations of com- plex hybrid song phenotypes are presented. The biogeography of N. similaris is discussed in light of historical changes in forest composition on the southeastern Coastal Plain. Key words: Acoustic behavior, sexual signals, hybridization, hybrid zone, parapatric distribution, speciation Introduction The cryptotympanine cicadas of North America have received much recent attention with the publication of comprehensive molecular and cladistic phylogenies and the reassignment of all former North American Tibicen Latreille species into new genera (Hill et al.