KCNK1 Antibody Cat. No.: 8017

KCNK1 Antibody

Immunohistochemistry of KCNK1 in mouse brain tissue Immunofluorescence of KCNK1 in mouse brain tissue with with KCNK1 antibody at 5 μg/ml. KCNK1 antibody at 20 μg/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: HOMOLOGY: (100%), Guinea pig: (100%)

KCNK1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KCNK1. IMMUNOGEN:

The immunogen is located within amino acids 220 - 270 of KCNK1.

TESTED APPLICATIONS: ELISA, IF, IHC-P, WB

September 30, 2021 1 https://www.prosci-inc.com/kcnk1-antibody-8017.html KCNK1 antibody can be used for detection of KCNK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

KCNK1 antibody is human, mouse and rat reactive. KCNK1 antibody is predicted to not SPECIFICITY: cross-react with other KCNK proteins.

POSITIVE CONTROL: 1) Cat. No. 1212 - 3T3 Cell Lysate

Predicted: 37 kDa PREDICTED MOLECULAR WEIGHT: Observed: 35 kDa

Properties

PURIFICATION: KCNK1 antibody is affinity chromatography purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: KCNK1 antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION: 1 mg/mL

KCNK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year.

Additional Info

OFFICIAL SYMBOL: KCNK1

Potassium channel subfamily K member 1, DPK, HOHO, K2P1, K2p1.1, KCNO1, TWIK-1, ALTERNATE NAMES: TWIK1

ACCESSION NO.: NP_002236

PROTEIN GI NO.: 4504847

GENE ID: 3775

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 30, 2021 2 https://www.prosci-inc.com/kcnk1-antibody-8017.html The potassium channel, subfamily K, member 1 (KCNK1), is member of the superfamily of potassium channel proteins containing two pore-forming P domains (1,2). KCNK1 is a ubiquitous human weakly inward rectifying K+ channel that is widely expressed in human BACKGROUND: tissues, and particularly abundant in brain and heart (1). Both KCNK1 and the related protein KCNK2 contribute significantly to the passive conductance of hippocampal astrocytes (3).

1) Lesage F, Guillemare E, Fink M, et al. TWIK-1, a ubiquitous human weakly inward REFERENCES: rectifying K+ channel with a novel structure. EMBO J. 1996; 15:1004-11.

2) Goldstein SA, Bockenhauer D, O’Kelly I, et al. Potassium leak channels and the KCNK family of two-P domain subunits. Nat. Rev. Neurosci. 2001; 2:175-84.

3) Zhou M, Xu G, Xie M, et al. TWIK-1 and TREK-2 are potassium channels contributing significantly to astrocyte passive conductance in rat hippocampal slides. J. Neurosci. 2009; 29:8551-64.

ANTIBODIES FOR RESEARCH USE ONLY.

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