Mouse Insig2 Conditional Knockout Project (CRISPR/Cas9)
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Goat Anti-PCK1 / PEPCKC (Internal) Antibody Peptide-Affinity Purified Goat Antibody Catalog # Af1796b
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Goat Anti-PCK1 / PEPCKC (internal) Antibody Peptide-affinity purified goat antibody Catalog # AF1796b Specification Goat Anti-PCK1 / PEPCKC (internal) Antibody - Product Information Application IHC Primary Accession P35558 Other Accession NP_002582, 5105 Reactivity Human Predicted Mouse, Rat, Pig, Dog, Cow Host Goat Clonality Polyclonal Concentration 100ug/200ul Isotype IgG Calculated MW 69195 AF1796b (2 µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, Goat Anti-PCK1 / PEPCKC (internal) Antibody - AP-staining. Additional Information Goat Anti-PCK1 / PEPCKC (internal) Gene ID 5105 Antibody - Background Other Names Phosphoenolpyruvate carboxykinase, This gene is a main control point for the cytosolic [GTP], PEPCK-C, 4.1.1.32, PCK1, regulation of gluconeogenesis. The cytosolic PEPCK1 enzyme encoded by this gene, along with GTP, catalyzes the formation of Format phosphoenolpyruvate from oxaloacetate, with 0.5 mg IgG/ml in Tris saline (20mM Tris the release of carbon dioxide and GDP. The pH7.3, 150mM NaCl), 0.02% sodium azide, expression of this gene can be regulated by with 0.5% bovine serum albumin insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of Storage cytosolic phosphoenolpyruvate carboxykinase Maintain refrigerated at 2-8°C for up to 6 deficiency. A mitochondrial isozyme of the months. For long term storage store at encoded protein also has been characterized. -20°C in small aliquots to prevent freeze-thaw cycles. Goat Anti-PCK1 / PEPCKC (internal) Antibody - References Precautions Goat Anti-PCK1 / PEPCKC (internal) Antibody COMMON VARIANTS IN 40 GENES ASSESSED is for research use only and not for use in FOR DIABETES INCIDENCE AND RESPONSE TO diagnostic or therapeutic procedures. -
Genome-Wide Linkage and Admixture Mapping of Type 2 Diabetes In
ORIGINAL ARTICLE Genome-Wide Linkage and Admixture Mapping of Type 2 Diabetes in African American Families From the American Diabetes Association GENNID (Genetics of NIDDM) Study Cohort Steven C. Elbein,1,2 Swapan K. Das,1,2 D. Michael Hallman,3 Craig L. Hanis,3 and Sandra J. Hasstedt4 OBJECTIVE—We used a single nucleotide polymorphism (SNP) map in a large cohort of 580 African American families to identify regions linked to type 2 diabetes, age of type 2 diabetes ype 2 diabetes is marked by a clear genetic diagnosis, and BMI. propensity, a high concordance in identical twins, tendencies for both diabetes and age of RESEARCH DESIGN AND METHODS—After removing outli- onset to be familial (1), and marked differences ers and problematic samples, we conducted linkage analysis T in prevalence among ethnic groups (2). Despite consider- using 5,914 SNPs in 1,344 individuals from 530 families. Linkage analysis was conducted using variance components for type 2 able evidence for a genetic predisposition, unraveling the diabetes, age of type 2 diabetes diagnosis, and BMI and nonpara- genetic etiology has been daunting, with few confirmed metric linkage analyses. Ordered subset analyses were con- genes identified from genome-wide linkage scans. Recent ducted ranking on age of type 2 diabetes diagnosis, BMI, waist successes with genome-wide association scans (3) have circumference, waist-to-hip ratio, and amount of European ad- greatly increased the number of confirmed genetic loci, mixture. Admixture mapping was conducted using 4,486 markers but these successes have been limited primarily to Cauca- not in linkage disequilibrium. -
Expression of Candidate Genes Associated with Obesity in Peripheral White Blood Cells of Mexican Children
Basic research Expression of candidate genes associated with obesity in peripheral white blood cells of Mexican children Marcela Ulloa-Martínez1, Ana I. Burguete-García2, Selvasankar Murugesan1,3, Carlos Hoyo-Vadillo3, Miguel Cruz-Lopez4, Jaime García-Mena1 1Departamento de Genética y Biología Molecular, Centro de Investigación y de Corresponding author: Estudios Avanzados del IPN, México, México Jaime García-Mena PhD 2Dirección de Infecciones Crónicas y Cáncer, CISEI, Instituto Nacional de Salud Pública, Departamento de Genética México, México y Biología Molecular 3Departamento de Farmacología, Centro de Investigación y de Estudios Avanzados del Centro de Investigación IPN, México, México y de Estudios Avanzados 4Unidad Unidad de Investigación Médica en Bioquímica, Centro Médico Nacional del IPN Siglo XXI, Instituto Mexicano del Seguro Social, México, México Av IPN #2508 Col Zacatenco Submitted: 3 December 2014 07360 México, México Accepted: 4 February 2015 Phone: +52 55 5747-3800 ext. 5328 Arch Med Sci 2016; 12, 5: 968–976 E-mail: [email protected] DOI: 10.5114/aoms.2016.58126 Copyright © 2016 Termedia & Banach Abstract Introduction: Obesity is a chronic, complex, and multifactorial disease, char- acterized by excess body fat. Diverse studies of the human genome have led to the identification of susceptibility genes that contribute to obesity. However, relatively few studies have addressed specifically the association between the level of expression of these genes and obesity. Material and methods: We studied 160 healthy and obese unrelated Mexi- can children aged 6 to 14 years. We measured the transcriptional expression of 20 genes associated with obesity, in addition to the biochemical parame- ters, in peripheral white blood cells. -
Human Induced Pluripotent Stem Cell–Derived Podocytes Mature Into Vascularized Glomeruli Upon Experimental Transplantation
BASIC RESEARCH www.jasn.org Human Induced Pluripotent Stem Cell–Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation † Sazia Sharmin,* Atsuhiro Taguchi,* Yusuke Kaku,* Yasuhiro Yoshimura,* Tomoko Ohmori,* ‡ † ‡ Tetsushi Sakuma, Masashi Mukoyama, Takashi Yamamoto, Hidetake Kurihara,§ and | Ryuichi Nishinakamura* *Department of Kidney Development, Institute of Molecular Embryology and Genetics, and †Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan; ‡Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Hiroshima, Japan; §Division of Anatomy, Juntendo University School of Medicine, Tokyo, Japan; and |Japan Science and Technology Agency, CREST, Kumamoto, Japan ABSTRACT Glomerular podocytes express proteins, such as nephrin, that constitute the slit diaphragm, thereby contributing to the filtration process in the kidney. Glomerular development has been analyzed mainly in mice, whereas analysis of human kidney development has been minimal because of limited access to embryonic kidneys. We previously reported the induction of three-dimensional primordial glomeruli from human induced pluripotent stem (iPS) cells. Here, using transcription activator–like effector nuclease-mediated homologous recombination, we generated human iPS cell lines that express green fluorescent protein (GFP) in the NPHS1 locus, which encodes nephrin, and we show that GFP expression facilitated accurate visualization of nephrin-positive podocyte formation in -
Insig2 Polyclonal Antibody Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP58480
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Insig2 Polyclonal Antibody Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP58480 Specification Insig2 Polyclonal Antibody - Product Information Application WB Primary Accession Q9Y5U4 Reactivity Rat, Pig, Dog, Cow Host Rabbit Clonality Polyclonal Calculated MW 24778 Insig2 Polyclonal Antibody - Additional Information Gene ID 51141 Other Names Insulin-induced gene 2 protein, INSIG-2, INSIG2 {ECO:0000303|PubMed:12242332, Sample: Liver (Mouse) Lysate at 40 ug ECO:0000312|HGNC:HGNC:20452} Primary: Anti-Insig2 (bs-6625R) at 1/300 dilution Format Secondary: HRP conjugated Goat-Anti-rabbit 0.01M TBS(pH7.4) with 1% BSA, 0.09% IgG (bs-0295G-HRP) at 1/5000 dilution (W/V) sodium azide and 50% Glyce Predicted band size: 25 kD Observed band size: 23 kD Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃. Insig2 Polyclonal Antibody - Protein Information Name INSIG2 {ECO:0000303|PubMed:12242332, ECO:0000312|HGNC:HGNC:20452} Function Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR (PubMed:<a href="http://www.unip rot.org/citations/12242332" target="_blank">12242332</a>, Page 1/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 PubMed:<a href="http://www.uniprot.org/ci tations/16606821" target="_blank">16606821</a>, PubMed:<a href="http://www.uniprot.org/ci tations/32322062" target="_blank">32322062</a>). -
WMC-79, a Potent Agent Against Colon Cancers, Induces Apoptosis Through a P53-Dependent Pathway
1617 WMC-79, a potent agent against colon cancers, induces apoptosis through a p53-dependent pathway Teresa Kosakowska-Cholody,1 Introduction 1 2 W. Marek Cholody, Anne Monks, The bisimidazoacridones are bifunctional antitumor Barbara A. Woynarowska,3 agents with strong selectivity against colon cancers (1, 2). and Christopher J. Michejda1 Recent studies of the effect of bisimidazoacridones on sensitive colon tumors cells revealed that these com- 1 Molecular Aspects of Drug Design, Structural Biophysics pounds act as cytostatic agents that completely arrest cell Laboratory, Center for Cancer Research; 2Screening Technologies Branch, Laboratory of Functional Genomics, Science Applications growth at G1 and G2-M check points but do not trigger International Corporation, National Cancer Institute at Frederick, cell death even at high concentrations (10 Amol/L; ref. 3). Frederick, Maryland; and 3Department of Radiation Oncology, The chemical structure of bisimidazoacridones is symmet- University of Texas Health Science Center, San Antonio, Texas rical in that it consists of two imidazoacridone moieties held together by linkers of various lengths and rigidities. Abstract We recently reported on the synthesis of unsymmetrical variants of the original bisimidazoacridones (4). WMC-79 WMC-79 is a synthetic agent with potent activity (Fig. 1), a compound consisting of an imidazoacridone against colon and hematopoietic tumors. In vitro, the moiety linked to a 3-nitronaphthalimide moiety via agent is most potent against colon cancer cells that 1,4-bispropenopiperazine linker, was found to be a potent carry the wild-type p53 tumor suppressor gene (HCT- but selective cytotoxic agent in a variety of tumor cell f 116 and RKO cells: GI50 <1 nmol/L, LC50 40 nmol/L). -
Original Article Genetic Polymorphisms of INSIG2 Were Associated with Coronary Artery Disease in Uygur Chinese Population in Xinjiang, China
Int J Clin Exp Pathol 2016;9(8):8575-8585 www.ijcep.com /ISSN:1936-2625/IJCEP0026579 Original Article Genetic polymorphisms of INSIG2 were associated with coronary artery disease in Uygur Chinese population in Xinjiang, China Dilare Adi1,2*, Yun Wu3*, Xiang Xie1,2, Gulinaer Baituola1,2, Fen Liu2, Ying-Ying Zheng1,2, Yi-Ning Yang1,2, Xiao-Mei Li1,2, Ding Huang1,2, Xiang Ma1,2, Bang-Dang Chen2, Min-Tao Gai2, Xiao-Cui Chen2, Zhen-Yan Fu1,2, Yi-Tong Ma1,2 1Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, People’s Republic of China; 2Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, People’s Republic of China; 3Department of General Medicine, First Affiliated Hospital of Xinjiang Medical University, Urumqi, People’s Republic of China. *Equal contributors. Received February 24, 2016; Accepted May 21, 2016; Epub August 1, 2016; Published August 15, 2016 Abstract: Background: Dyslipidemia is a major and independent risk factor for the development of Coronary artery disease (CAD). The protein which is encoded by insulin induced gene2 (INSIG2) plays an important role in the media- tion of the feedback control of cholesterol synthesis, lipogenesis and glucose homeostasis. The aim of the present study was to assess the association between the human INSIG2 gene and CAD in Han Chinese and Uygur Chinese population of Xinjiang, China. Methods: A total of 832 CAD patients (334 Han, 498 Uygur) and 919 controls (346 Han, 573Uygur) were selected for the present Case-control study. Three tagging SNPs (rs1261829, rs21613329 and rs17047757) of INSIG2 gene were genotyped using TaqMan® assays from Applied Biosystems following the manufacturer’s instructions and analyzed in an ABI 7900HT Fast Real-Time PCR System. -
Activation of Sterol Regulatory Element‑Binding Proteins in Mice Exposed to Perfluorooctanoic Acid for 28 Days
Arch Toxicol DOI 10.1007/s00204-014-1322-7 MOLECULAR TOXICOLOGY Activation of sterol regulatory element‑binding proteins in mice exposed to perfluorooctanoic acid for 28 days Shengmin Yan · Jianshe Wang · Jiayin Dai Received: 10 April 2014 / Accepted: 21 July 2014 © Springer-Verlag Berlin Heidelberg 2014 Abstract Perfluoroalkyl acids are widely used in numer- lipid content and PPARα in Hepa 1-6 cells after exposure ous industrial and commercial applications due to their to PFOA for 72 h but SREBPs were not activated in vitro. unique physical and chemical characteristics. Although per- These results demonstrated that SREBPs were maturated fluorooctanoic acid (PFOA) is associated with hepatomeg- by activating the miR-183-96-182 cluster-SREBP regula- aly through peroxisome proliferator-activated receptor α tory loop in PFOA-exposed mouse liver. (PPARα) activation, liver fat accumulation and changes in gene expression related to fatty acid metabolism could still Keywords SREBP · Lipid metabolism · PPAR · be found in PPARα-null mice exposed to PFOA. To explore Perfluorononanoic acid the potential effects of PFOA on sterol regulatory element- binding proteins (SREBPs) activity, male mice were dosed with either Milli-Q water or PFOA at doses of 0.08, 0.31, Introduction 1.25, 5, and 20 mg/kg/day by gavage for 28 days. Liver total cholesterol concentrations and PFOA contents showed Perfluoroalkyl acids (PFAAs) are a family of anthropo- a dose-dependent decrease and increase, respectively. Tran- genic compounds widely used in industrial and commer- scriptional activity of PPARα and SREBPs was signifi- cial applications due to their unique physical and chemical cantly enhanced in livers. -
Gene Interactions of the INSIG1 and INSIG2 in Metabolic Syndrome in Schizophrenic Patients Treated with Atypical Antipsychotics
The Pharmacogenomics Journal (2012) 12, 54–61 & 2012 Macmillan Publishers Limited. All rights reserved 1470-269X/12 www.nature.com/tpj ORIGINAL ARTICLE Gene–gene interactions of the INSIG1 and INSIG2 in metabolic syndrome in schizophrenic patients treated with atypical antipsychotics Y-J Liou1,2,3,8, YM Bai1,2,8, The use of atypical antipsychotics (AAPs) is associated with increasing the risk 4,5 6 6 of the metabolic syndrome (MetS), which is an important risk factor for E Lin , J-Y Chen , T-T Chen , cardiovascular disease and diabetes. Two insulin-induced gene (INSIG) 1,2,7 1,2 C-J Hong and S-J Tsai isoforms, designated INSIG-1 and INSIG-2 encode two proteins that mediate feedback control of lipid metabolism. In this genetic case–control study, we 1Department of Psychiatry, Taipei Veterans General Hospital, Taipei, Taiwan; 2Division of investigated whether the common variants in INSIG1 and INSIG2 genes were Psychiatry, School of Medicine, National Yang- associated with MetS in schizophrenic patients treated with atypical Ming University, Taipei, Taiwan; 3Institute of antipsychctics. The study included 456 schizophrenia patients treated with Clinical Medicine, School of Medicine, National 4 clozapine (n ¼ 171), olanzapine (n ¼ 91) and risperidone (n ¼ 194), for an Yang-Ming University, Taipei, Taiwan; Vita ± Genomics, Taipei, Taiwan; 5Graduate Institute of average of 45.5 27.6 months. The prevalence of MetS among all subjects Clinical Medical Science, China Medical was 22.8% (104/456). Two single-nucleotide polymorphisms (SNPs) of the University, Taichung, Taiwan; 6Department of INSIG1 gene and seven SNPs of the INSIG2 gene were chosen as haplotype- Psychiatry, Yuli Veterans Hospital, Yuli, Hualien, tagging SNPs. -
INSIG2 Variants, Dietary Patterns and Metabolic Risk in Samoa
European Journal of Clinical Nutrition (2013) 67, 101–107 & 2013 Macmillan Publishers Limited All rights reserved 0954-3007/13 www.nature.com/ejcn ORIGINAL ARTICLE INSIG2 variants, dietary patterns and metabolic risk in Samoa A Baylin1,2, R Deka3, J Tuitele4, S Viali5, DE Weeks6,7 and ST McGarvey2,8 BACKGROUND/OBJECTIVES: Association of insulin-induced gene 2 (INSIG2) variants with obesity has been confirmed in several but not all follow-up studies. Differences in environmental factors across populations may mask some genetic associations and therefore gene–environment interactions should be explored. We hypothesized that the association between dietary patterns and components of the metabolic syndrome could be modified by INSIG2 variants. SUBJECTS/METHODS: We conducted a longitudinal study of adiposity and cardiovascular disease risk among 427 and 290 adults from Samoa and American Samoa (1990–1995). Principal component analysis on food items from a validated food frequency questionnaire was used to identify neotraditional and modern dietary patterns. We explored gene–dietary pattern interactions with the INSIG2 variants rs9308762 and rs7566605. RESULTS: Results for American Samoans were mostly nonsignificant. In Samoa, the neotraditional dietary pattern was associated with lower triglycerides, body mass index (BMI), waist circumference, systolic and diastolic blood pressure and fasting glucose (all P-for-trendo0.05). The modern pattern was significantly associated with higher triglycerides, BMI, waist circumference and lower high-density lipoprotein-cholesterol (all P-for-trendo0.05). A significant interaction for triglycerides was found between the modern pattern and the rs9308762 polymorphism (P ¼ 0.04). Those from Samoa consuming the modern pattern have higher triglycerides if they are homozygous for the rs9308762 C allele. -
Open Mithun Shah.Pdf
The Pennsylvania State University The Graduate School Department of Molecular Medicine ROLE OF SPHINGOLIPID SIGNALING IN PATHOGENESIS OF LARGE GRANULAR LYMPHOCYTE LEUKEMIA A Dissertation in Molecular Medicine by Mithun Vinod Shah © 2009 Mithun Vinod Shah Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy May 2009 ii The dissertation of Mithun Vinod Shah was reviewed and approved* by the following: Thomas P. Loughran, Jr. Professor of Medicine Dissertation Advisor Co-Chair of Committee Rosalyn B. Irby Assistant Professor of Medicine Co-chair of Committee Gary Clawson Professor of Pathology, and Biochemistry and Molecular Biology Edward J. Gunther Assistant Professor of Medicine Charles H. Lang Director, Molecular Medicine Graduate Program *Signatures are on file in the Graduate School iii ABSTRACT Large granular lymphocyte (LGL) leukemia is a disorder of mature cytotoxic cells. LGL leukemia is characterized by accumulation of cytotoxic cells in blood and infiltration in bone marrow and other tissues. Leukemic LGL could arise from expansion of either CD3+ CD8+ T-cells (T-cell LGL leukemia or T-LGL leukemia) or those arising from CD3- natural killer (NK)-cells (NK-cell LGL leukemia or NK-LGL leukemia). LGL leukemia is a rare disorder consisting of less than 5% of non-B cell leukemia. Clinically, LGL leukemia can manifest along a spectrum of disorders ranging from slowly progressing indolent disorder to an aggressive leukemia that could be fatal within months. About fifty percent of LGL leukemia patients also present with variety of autoimmune conditions, rheumatoid arthritis being the most common one. Normally, following antigen clearance, cytotoxic T-lymphocytes (CTL) become sensitive to Fas-mediated apoptosis resulting in activation-induced cell death (AICD). -
Interaction Networks of Lithium and Valproate Molecular Targets Reveal a Striking Enrichment of Apoptosis Functional Clusters and Neurotrophin Signaling
The Pharmacogenomics Journal (2012) 12, 328–341 & 2012 Macmillan Publishers Limited. All rights reserved 1470-269X/12 www.nature.com/tpj ORIGINAL ARTICLE Interaction networks of lithium and valproate molecular targets reveal a striking enrichment of apoptosis functional clusters and neurotrophin signaling A Gupta1, TG Schulze2, The overall neurobiological mechanisms by which lithium and valproate 3 1 stabilize mood in bipolar disorder patients have yet to be fully defined. The V Nagarajan , N Akula , therapeutic efficacy and dissimilar chemical structures of these medications 1 1 W Corona , X-y Jiang , suggest that they perturb both shared and disparate cellular processes. To N Hunter1, FJ McMahon1 and investigate key pathways and functional clusters involved in the global action SD Detera-Wadleigh1 of lithium and valproate, we generated interaction networks formed by well- supported drug targets. Striking functional similarities emerged. Intersecting 1Human Genetics Branch, National Institute of nodes in lithium and valproate networks highlighted a strong enrichment Mental Health, National Institutes of Health, of apoptosis clusters and neurotrophin signaling. Other enriched pathways 2 Bethesda, MD, USA; Department of Psychiatry included MAPK, ErbB, insulin, VEGF, Wnt and long-term potentiation and Psychotherapy, University Medical Center, Georg-August-Universita¨t, Go¨ttingen, Germany indicating a widespread effect of both drugs on diverse signaling systems. and 3Bioinformatics and Computational MAPK1/3 and AKT1/2 were the most preponderant nodes across pathways Biosciences Branch, Office of Cyber Infrastructure suggesting a central role in mediating pathway interactions. The conver- and Computational Biology, National Institute of gence of biological responses unveils a functional signature for lithium and Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA valproate that could be key modulators of their therapeutic efficacy.