Phospho ERBB2 / HER2 Antibody Duo (Total, Py1221/1222)

ARG30034 Package: 1 pair Phospho ERBB2 / HER2 Antibody Duo (Total, pY1221/1222)

Component

Cat. No. Component Name Host clonality Reactivity Application Package

ARG51067 anti-ERBB2 / HER2 Rabbit pAb Hu ICC/IF, IHC-P, WB 50 μl antibody

ARG51579 anti-ERBB2 / HER2 Rabbit pAb Hu WB, ICC/IF 50 μl phospho (Tyr1221 / Tyr1222) antibody

Summary

Product Description Receptor tyrosine-protein kinase erbB-2, a 185 kDa protein, also known as CD340 (cluster of differentiation 340), proto-oncogene Neu, Erbb2 (rodent), or ERBB2 (human) is a protein that in humans is encoded by the ERBB2 gene. The ERBB2 gene is also frequently called HER2 (from human epidermal growth factor receptor 2) or HER2/neu.

HER2 is a member of the epidermal growth factor receptor (EGFR/ERBB) family. HER2 has been shown to play an important role cancer development, metastasis and chemotherapeutic resistance in many kinds of multiple cancers including, breast cancer, lung cancer, gastric cancer, ovarian cancer and so on through its overexpression or mutation. HER2 is also become an important therapeutic target for cancers.

Tyr1248 and Tyr1221/1222 are the major autophosphorylation sites on ErbB2, and the phosphorylation on these sites correct to the activity and signaling transduction of HER2.

Phosphorylation at tyrosine 1221/1222 also has been approved to improve the prediction of poor survival for breast cancer patients with hormone receptor-positive tumors. Thus, both HER2 and phosphorylated HER2 are important targets for cancer diagnosis.

Target Name ERBB2 / HER2

Alternate Names Phospho ERBB2 / HER2 antibody; Phospho erb-b2 receptor tyrosine kinase 2 antibody; Phospho ERBB2 antibody; ERBB2 / HER2 antibody; ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody

Properties

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Phospho erb-b2 receptor tyrosine kinase 2 (ERBB2) Antibody Duo (Total, pY1221/1222)

Research Area Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody

www.arigobio.com 1/3 Images

ARG51067 anti-HER2 antibody WB image

Western Blot: extracts from SKOV3 and MDA cells stained with anti- HER2 antibody ARG51067.

ARG51579 anti-HER2 phospho (Tyr1221/Tyr1222) antibody WB image

Western Blot: extracts from MDA cells untreated or treated with EGF stained with anti-HER2 (phospho Tyr1221/Tyr1222) antibody ARG51579.

ARG51579 anti-HER2 phospho (Tyr1221/Tyr1222) antibody WB image

Western Blot: extracts from HeLa cells, treated with EGF or calf intestinal phosphatase (CIP), stained with anti-HER2 (phospho Tyr1221/Tyr1222) antibody ARG51579.

ARG51067 anti-HER2 antibody IHC-P image

Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-HER2 antibody ARG51067 (left) or the same antibody preincubated with blocking peptide (right).

www.arigobio.com 2/3 ARG51067 anti-HER2 antibody ICC/IF image

Immunofluorescence: methanol-fixed MCF cells stained with anti- HER2 antibody ARG51067.

ARG51579 anti-HER2 phospho (Tyr1221/Tyr1222) antibody ICC/IF image

Immunofluorescence: methanol-fixed MCF cells stained with anti- HER2 (phospho Tyr1221/Tyr1222) antibody ARG51579.