BIOLOGY 101
CHAPTER 8: An Introduction to Metabolism: Energy of Life An Introduction to Metabolism: Energy of Life
CONCEPTS: • 8.1 An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics • 8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously • 8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions • 8.4 Enzymes speed up metabolic reactions by lowering energy barriers • 8.5 Regulation of enzyme activity helps control metabolism
Can you describe or explain these concepts? An Introduction to Metabolism: Energy of Life
8.1 An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics • The sum total of an organism’s chemical reactions is called metabolism. An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 The chemistry of life is organized into metabolic pathways • A metabolic pathway begins with a specific molecule, which is then altered in a series of defined steps to form a specific product. An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 The chemistry of life is organized into metabolic pathways • A metabolic pathway begins with a specific molecule, which is then altered in a series of defined steps to form a specific product. ✓ A specific enzyme catalyzes each step of the pathway. • Catabolic pathways release energy by breaking down complex molecules to simpler compounds. o The energy released by catabolic pathways becomes available to do the work of the cell, such as ciliary beating or membrane transport. • Anabolic pathways require energy derived from catabolic pathways to build complex molecules from simple ones An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 Organisms transform energy • Energy is the capacity to cause change. o In everyday life, some forms of energy can be used to do work—that is, to move matter against opposing forces, such as gravity and friction.
• Energy exists in various forms, and cells transform energy from one type to another. An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 Organisms transform energy • Kinetic energy is the energy associated with the relative motion of objects. o Objects in motion can perform work by imparting motion to other matter.
✓ Work = to move matter (or cause change) against opposing forces o Thermal energy is kinetic energy associated with the random movement of atoms or molecules. ✓ Heat is caused by thermal energy o Mechanical Energy is derived from particles in motion o Electrical Energy exists in the form of membrane potentials that exist in cells An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 Organisms transform energy • Potential energy is the energy that matter possesses because of its location or structure. o Water behind a dam possesses energy because of its altitude above sea level.
o Molecules possess energy because of the arrangement of electrons in the bonds between their atoms. o Chemical energy is a term used by biologists to refer to the potential energy available for release in a chemical reaction. An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 The energy transformations of life are subject to two laws of thermodynamics • Organisms are open systems: They absorb energy—light or chemical energy in the form of organic
molecules—and release heat and metabolic waste products, such as urea or CO2, to their surroundings. • Two laws of thermodynamics govern energy transformations in organisms and all other collections of matter An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 The energy transformations of life are subject to two laws of thermodynamics • The first law of thermodynamics states that the energy of the universe is constant: Energy can be transferred and transformed, but it cannot be created or destroyed. o The first law is also known as the principle of conservation of energy. o Plants do not produce energy; they transform light energy to chemical energy An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 The energy transformations of life are subject to two laws of thermodynamics • Energy transfers and transformations make the universe more disordered due to the loss of usable energy. • Entropy is a measure of disorder or randomness: The more random a collection of matter, the greater its entropy An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 The energy transformations of life are subject to two laws of thermodynamics • The second law of thermodynamics states: Every spontaneous energy transfer or transformation increases the entropy of the universe.
• Although order can increase locally (as in a cell), there is an unstoppable trend toward randomization of the universe.
• This randomizing requires energy – this energy is lost (unusable now) from the system An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 The energy transformations of life are subject to two laws of thermodynamics
o For a process to occur on its own, without outside help in the form of energy input, it must increase the entropy of the universe
• A spontaneous process is one that is energetically favorable. A process that cannot occur on its own is said to be nonspontaneous: it will happen only if energy is added to the system. An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 The energy transformations of life are subject to two laws of thermodynamics
• Living systems increase the entropy of their surroundings, even though they create ordered structures from less ordered starting materials. An Introduction to Metabolism: An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics 8.1 The energy transformations of life are subject to two laws of thermodynamics • Over evolutionary time, complex organisms have evolved from simpler ones. • This increase in organization does not violate the second law of thermodynamics.
o The entropy of a particular system, such as an organism, may decrease as long as the total entropy of the universe—the system plus its surroundings—increases. o Cells are open systems, both receiving and distributing energy and molecules Organisms are islands of low entropy in an increasingly random universe
Can you explain this statement? An Introduction to Metabolism: Energy of Life
8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously
❖ How can we determine which reactions occur spontaneously and which ones require an input of energy?
• The concept of free energy (symbolized by the letter G) is useful for measuring the spontaneity of a system.
✓ G stands for Gibbs Free Energy An Introduction to Metabolism: Energy of Life
8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously
• Free energy (G) is the portion of a system’s energy that can perform work when temperature and pressure are uniform throughout the system, as in a living cell.
o The key to understanding Free Energy (G), is that it is composed of the energy that can actually do work, as well as energy lost in reactions due to heat loss or entropy An Introduction to Metabolism: Energy of Life
8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously • The change in free energy, ∆G, can be calculated for any specific chemical reaction by applying the following equation: ∆G = ∆H – T∆S Change in Free Energy = Change in Enthalpy – (Temperature (K)) x (Change in Entropy)
• In this equation: • ∆H symbolizes the change in the system’s enthalpy (in biological systems, equivalent to total energy (or total heat content of the system) • ∆S is the change (loss or gain) in the system’s entropy; and • T is the absolute temperature in Kelvin (K) units (K = °C + 273) An Introduction to Metabolism: Energy of Life
8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously • The change in free energy, ∆G, can be calculated for any specific chemical reaction by applying the following equation: ∆G = ∆H – T∆S Change in Free Energy = Change in Enthalpy – (Temperature (K)) (Change in Entropy)
• In this equation: • ∆H symbolizes the change in the system’s enthalpy (in biological systems, equivalent to total energy (or total heat content of the system) • ∆S is the change (loss or gain) in the system’s entropy; and • T is the absolute temperature in Kelvin (K) units (K = °C + 273) An Introduction to Metabolism: Energy of Life
8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously • The change in free energy, ∆G, can be calculated for any specific chemical reaction by applying the following equation: ∆G = ∆H – T∆S
H would equal G, but the system is missing energy lost to heat and entropy
• Enthalpy measures the usable energy content of the system
• Enthalpy is an abstract term that relates to the usable internal energy in Joules/mol An Introduction to Metabolism: Energy of Life
8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously
• WHY IS THE CHANGE IN ENTROPY SUBTRACTED IF IT IS A POSITIVE VALUE? ∆G = ∆H – T∆S
• This is explained by understanding that in spontaneous reactions, the change in free energy is NEGATIVE
1. This means the combination of enthalpy change and entropy change must both be negative.
2. Additionally, the energy from heat and entropy are ‘lost’ from the system – so they must be subtracted An Introduction to Metabolism: Energy of Life
8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously • ∆G must have a negative value (∆G < 0) in order for a process to be spontaneous. o In other words, every spontaneous process decreases the system’s free energy, and processes that have positive or zero ∆G are never spontaneous
o Because it has less free energy, the system in its final state is less likely to change and is therefore more stable than it was previously
✓ Spontaneous reactions that release energy are called exergonic, while those that are nonspontaneous (requiring energy) are called endergonic An Introduction to Metabolism: Energy of Life
8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously • A system at equilibrium is at maximum stability. o In a chemical reaction at equilibrium, the rates of forward and backward reactions are equal, and there is no change in the relative concentrations of products or reactants. o At equilibrium, ∆G = 0, and the system can do no work. An Introduction to Metabolism: Energy of Life
8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously • A process is spontaneous and can perform work only when it is moving toward equilibrium • Movements away from equilibrium are nonspontaneous and will have a positive ∆G An Introduction to Metabolism: Energy of Life
8.2 Chemical reactions can be classified as either exergonic or endergonic • An exergonic reaction proceeds spontaneously because the reactants already have enough stored energy
• An endergonic reaction is not spontaneous because the reactants are lacking sufficient stored energy to complete the reaction An Introduction to Metabolism: Energy of Life
8.2 Chemical reactions can be classified as either exergonic or endergonic • An exergonic reaction proceeds with a net change of free energy; G is negative. o The magnitude of G for an exergonic reaction is the maximum amount of work the reaction can perform. • An endergonic reaction is one that absorbs free energy from its surroundings. o Endergonic reactions must add Free Energy to reactants; G is positive.
o Endergonic reactions are nonspontaneous
An Introduction to Metabolism: Energy of Life
8.2 Chemical reactions can be classified as either exergonic or endergonic • An endergonic reaction is one that absorbs free energy from its surroundings. o Endergonic reactions require energy; G is positive. o Endergonic reactions are nonspontaneous, and the magnitude of G is the quantity of energy required to drive the reaction An Introduction to Metabolism: Energy of Life
8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions • A cell does three main kinds of work: 1. Chemical work, pushing endergonic reactions such as the synthesis of polymers from monomers; 2. Transport work, pumping substances across membranes against the direction of spontaneous movement; 3. Mechanical work, such as the beating of cilia, contraction of muscle cells, and movement of chromosomes during cellular reproduction
This is what -G can accomplish in cells An Introduction to Metabolism: Energy of Life
8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions
So if work is done inside cells…
Where does the energy to power endergonic reactions come from? An Introduction to Metabolism: Energy of Life
8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions • Cells manage their energy resources to do this work by energy coupling, using an exergonic process to drive an endergonic one. • ATP is responsible for mediating most energy coupling in cells, and in most cases it acts as the immediate source of energy that powers cellular work
• ATP is the energetic link between Exergonic and Endergonic reactions! An Introduction to Metabolism: Energy of Life
8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions • Cells manage their energy resources to do this work by energy coupling, using an exergonic process to drive an endergonic one. • ATP is responsible for mediating most energy coupling in cells, and in most cases it acts as the immediate source of energy that powers cellular work
• The formation of Glutamine is not spontaneous (+3.4G) An Introduction to Metabolism: Energy of Life
8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions • Cells manage their energy resources to do this work by energy coupling, using an exergonic process to drive an endergonic one. • ATP is responsible for mediating most energy coupling in cells, and in most cases it acts as the immediate source of energy that powers cellular work
• The formation of Glutamine is not spontaneous (+3.4G) • Phosphorylation by ATP changes the G from + to - Because it adds 7.3 Kcal/mol to the reactants • ATP brings the reaction to -3.9G An Introduction to Metabolism: Energy of Life
8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions • Cells manage their energy resources to do this work by energy coupling, using an exergonic process to drive an endergonic one. • ATP is responsible for mediating most energy coupling in cells, and in most cases it acts as the immediate source of energy that powers cellular work
• The formation of Glutamine is not spontaneous (+3.4G) • Phosphorylation by ATP decreases system by -7.3 G
• ATP brings the reaction to -3.9G An Introduction to Metabolism: Energy of Life
8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions • Cells manage their energy resources to do this work by energy coupling, using an exergonic process to drive an endergonic one. • ATP is responsible for mediating most energy coupling in cells, and in most cases it acts as the immediate source of energy that powers cellular work
✓ Through energy coupling, ATP can take an endergonic reaction and make it exergonic! Energetic Coupling by ATP
Why does adding ATP change the system by -7.3kcal/mol? Energetic Coupling by ATP
Why does adding ATP change the system by -7.3kcal/mol? Energetic Coupling by ATP
Why does adding ATP change the system by -7.3kcal/mol?
This is the energy resulting from the hydrolysis of ATP to ADP + Pi (which is There are 2 reactions occurring here, one exergonic – yielding -7.3Kcal/mol) yielding ∆G of +3.4 Kcal/mol, and another yielding ∆G of -7.3Kcal/mol Energetic Coupling by ATP
Why does adding ATP change the system by -7.3kcal/mol? Energetic Coupling by ATP
Why does adding ATP change the system by -7.3kcal/mol? An Introduction to Metabolism: Energy of Life
8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions • Cells manage their energy resources to do this work by energy coupling, using an exergonic process to drive an endergonic one. • ATP is responsible for mediating most energy coupling in cells, and in most cases it acts as the immediate source of energy that powers cellular work
✓ Through energy coupling, ATP can take an endergonic reaction and make it exergonic! An Introduction to Metabolism: ATP powers cellular work by coupling exergonic reactions to endergonic reactions
8.3 ATP mediates most energy coupling in cells • Under standard conditions, ∆G = -7.3 kcal/mol An Introduction to Metabolism: ATP powers cellular work by coupling exergonic reactions to endergonic reactions
8.3 ATP mediates most energy coupling in cells • Although the phosphate bonds of ATP are sometimes referred to as high-energy phosphate bonds, these are actually fairly weak covalent bonds.
• The release of energy during the hydrolysis of ATP comes from the chemical change to a state of lower free energy change, not from the phosphate bonds themselves.
• Why does the hydrolysis of ATP yield so much energy?
o Each of the three phosphate groups has a negative charge. o These three like charges are crowded together, and their mutual repulsion contributes to the instability of this region of the ATP molecule. o The triphosphate tail of ATP is the chemical equivalent of a compressed spring. An Introduction to Metabolism: Energy of Life
8.4 Enzymes speed up metabolic reactions by lowering energy barriers • Spontaneous chemical reactions may occur so slowly as to be imperceptible. o The hydrolysis of table sugar (sucrose) to glucose and fructose is exergonic, with G = −7 kcal/mol. o Despite this, a solution of sucrose dissolved in sterile water may sit for years at room temperature with no appreciable hydrolysis. o If a small amount of the enzyme, sucrase, is added to a solution of sugar, all the sucrose is hydrolyzed within seconds An Introduction to Metabolism: Energy of Life
8.4 Enzymes speed up metabolic reactions by lowering energy barriers • An enzyme is a macromolecule that acts as a catalyst, a chemical agent that speeds up the rate of a reaction without being consumed by the reaction. o Enzymes are generally proteins (exception of ribozymes)
• Every chemical reaction involves bond breaking and bond forming. An Introduction to Metabolism: Energy of Life
8.4 Enzymes speed up metabolic reactions by lowering energy barriers • The initial investment of energy for starting a reaction is the free energy of activation, or activation energy (E ). A Energy required to rearrange chemical bonds o Activation energy is the amount of energy necessary to push the reactants over an energy barrier so that the “downhill” part of the reaction can begin.
o EA is often supplied in the form of thermal energy that the reactant molecules absorb from the surroundings. o This accelerates the reactant molecules, which then collide more often with more force making the breakage of bonds more likely.
✓ When reactants reach EA, they are in an unstable transition state An Introduction to Metabolism: Energy of Life
8.4 Enzymes speed up metabolic reactions by lowering energy barriers • The initial investment of energy for starting a reaction is the free energy of activation, or activation
energy (EA).
o This energy barrier is critical to life because it keeps exergonic reactions from running out of control o This means that some input of energy (or
lowering of needed EA) is required for reactions to proceed – it is a regulating device An Introduction to Metabolism: Energy of Life
8.4 Enzymes speed up metabolic reactions by lowering energy barriers
• For some processes, EA is not high, and the thermal energy provided by room temperature is sufficient for many reactants to reach the transition state
• In most cases, EA is high enough that the transition state is rarely reached and the reaction hardly proceeds at all. In these cases, the reaction will occur at a noticeable rate only if the reactants are heated. An Introduction to Metabolism: Energy of Life
8.4 Enzymes speed up metabolic reactions by lowering energy barriers • How are the barriers for selected reactions surmounted to allow cells to carry out the processes of life?
o Heat would speed up all reactions, not just those that are needed. o Excessive Heat also denatures proteins and can kill cells An Introduction to Metabolism: Energy of Life
8.4 Enzymes speed up metabolic reactions by lowering energy barriers
• Enzymes speed reactions by lowering the required EA. The transition state can then be reached even at moderate temperatures. An Introduction to Metabolism: Energy of Life
8.4 Enzymes speed up metabolic reactions by lowering energy barriers
• Enzymes speed reactions by lowering the needed EA. The transition state can then be reached even at moderate temperatures. An Introduction to Metabolism: Energy of Life
8.4 Enzymes speed up metabolic reactions by lowering energy barriers
• Enzymes speed reactions by lowering the required EA. The transition state can then be reached even at moderate temperatures. • Enzymes do not change G; they hasten reactions that would occur eventually. • Because enzymes are so selective, they determine which chemical processes will occur at any time. An Introduction to Metabolism: Energy of Life
8.4 Enzymes speed up metabolic reactions by lowering energy barriers
• Enzymes speed reactions by lowering the needed EA. The transition state can then be reached even at moderate temperatures. • Enzymes do not change G; they hasten reactions that would occur eventually. • Because enzymes are so selective, they determine which chemical processes will occur at any time.
How Enzymes Work [VIDEO] An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 Enzymes are substrate specific • The reactant that an enzyme acts on is the substrate. • The enzyme binds to a substrate, or substrates, forming an enzyme-substrate complex. An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 Enzymes are substrate specific • The reactant that an enzyme acts on is the substrate. • The enzyme binds to a substrate, or substrates, forming an enzyme-substrate complex.
• While the enzyme and substrate are bound, the catalytic action of the enzyme speeds the conversion of reactants to their respective products. An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 Enzymes are substrate specific • The reactant that an enzyme acts on is the substrate. • The enzyme binds to a substrate, or substrates, forming an enzyme-substrate complex.
• While the enzyme and substrate are bound, the catalytic action of the enzyme speeds the conversion of reactants to their respective products.
• Products are then released An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 Enzymes are substrate specific • The reactant that an enzyme acts on is the substrate. • The enzyme binds to a substrate, or substrates, forming an enzyme-substrate complex.
• While the enzyme and substrate are bound, the catalytic action of the enzyme speeds the conversion of reactants to their respective products.
• Products are then released
• The enzyme is then available for new substrates An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 Enzymes are substrate specific • Only a restricted region of the enzyme binds to the substrate. o The active site of an enzyme is typically a pocket or groove on the surface of the protein where catalysis occurs. o The active site is usually formed by only a few amino acids. The rest of the protein molecule provides a framework that determines the configuration of the active site. An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 Enzymes are substrate specific • The specificity of an enzyme is due to the fit between the active site and the substrate • The active site is not a rigid receptacle for the substrate. • As the substrate enters the active site, interactions between the chemical groups on the substrate and those on the side chains of the amino acids that form the active site cause the enzyme to change shape slightly. o This change leads to an induced fit that brings the chemical groups of the active site into position to catalyze the reaction. An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 A cell’s physical and chemical environment affects enzyme activity • The activity of an enzyme is affected by general environmental conditions, such as temperature and pH. • Each enzyme works best at certain optimal conditions, which favor the most active conformation for the enzyme molecule. An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 A cell’s physical and chemical environment affects enzyme activity • Temperature has a major impact on reaction rate. o As temperature increases, collisions between substrates and active sites occur more frequently as molecules move more rapidly. o As temperature increases further, thermal agitation begins to disrupt the weak bonds that stabilize the protein’s active conformation, and the protein denatures
• Each enzyme has an optimal temperature that allows the greatest number of molecular collisions and the fastest conversion of the reactants to product molecules.
o Most human enzymes have optimal temperatures of about 35–40°C An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 A cell’s physical and chemical environment affects enzyme activity • Temperature has a major impact on reaction rate. o As temperature increases, collisions between substrates and active sites occur more frequently as molecules move more rapidly. o As temperature increases further, thermal agitation begins to disrupt the weak bonds that stabilize the protein’s active conformation, and the protein denatures
• There is a delicate balance between an enzyme’s optimal temperature and increasing thermal energy too much
• Too much heat will denature proteins! An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 A cell’s physical and chemical environment affects enzyme activity • Maintenance of the active conformation of the enzyme requires a particular pH. o This optimal pH falls between pH 6–8 for most enzymes. o However, digestive enzymes in the stomach are designed to work best at pH 2, whereas those in the intestine have an optimal pH of 8. An Introduction to Metabolism: Enzymes speed up metabolic reactions by lowering energy barriers
8.4 A cell’s physical and chemical environment affects enzyme activity • Many enzymes require nonprotein helpers, called cofactors, for catalytic activity o Cofactors bind permanently or reversibly to the enzyme. o Some inorganic cofactors are zinc, iron, and copper in ionic form.
o Organic cofactors are called coenzymes. o Most vitamins are coenzymes or the raw materials from which coenzymes are made An Introduction to Metabolism: Regulation of enzyme activity helps control metabolism
8.5 Metabolic control often depends on allosteric regulation • In enzymes with multiple catalytic subunits, binding by a substrate molecule to one active site in a multisubunit enzyme triggers a shape change in all the subunits. o This mechanism, called cooperativity, amplifies the response of enzymes to substrates, priming the enzyme to accept additional substrates. o Cooperativity is considered to be “allosteric” regulation because binding of the substrate to one active site affects catalysis in a different active site. An Introduction to Metabolism: Regulation of enzyme activity helps control metabolism
8.5 Metabolic control often depends on allosteric regulation • The vertebrate oxygen-transport protein hemoglobin is not an enzyme, but is a classic example of cooperativity. o Hemoglobin is made up of four subunits, each with an oxygen-binding site. o The binding of an oxygen molecule to each binding site increases the affinity for oxygen of the remaining binding sites. o Under conditions of low oxygen, as in oxygen-deprived tissues, hemoglobin is less likely to bind oxygen and releases it where it is needed. o When oxygen is at higher levels, as in the lungs or gills, the protein has a greater affinity for oxygen as more binding sites are filled. An Introduction to Metabolism: Energy of Life
CONCEPTS: (Review) • 8.1 An organism's metabolism transforms matter and energy, subject to the laws of thermodynamics • 8.2 The free-energy change of a reaction tells us whether or not the reaction occurs spontaneously • 8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions • 8.4 Enzymes speed up metabolic reactions by lowering energy barriers • 8.5 Regulation of enzyme activity helps control metabolism
Can you describe or explain these processes?