Florida Fishery-Wide Reproductive Indices of Stone Crab, Menippe Mercenaria, and Their Application to Stock Assessment and Manag
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University of South Florida Scholar Commons Graduate Theses and Dissertations Graduate School March 2012 Florida Fishery-Wide Reproductive Indices of Stone Crab, Menippe mercenaria, and Their Application to Stock Assessment and Management Claire Elizabeth Crowley University of South Florida, [email protected] Follow this and additional works at: http://scholarcommons.usf.edu/etd Part of the Other Oceanography and Atmospheric Sciences and Meteorology Commons Scholar Commons Citation Crowley, Claire Elizabeth, "Florida Fishery-Wide Reproductive Indices of Stone Crab, Menippe mercenaria, and Their Application to Stock Assessment and Management" (2012). Graduate Theses and Dissertations. http://scholarcommons.usf.edu/etd/6821 This Dissertation is brought to you for free and open access by the Graduate School at Scholar Commons. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Scholar Commons. For more information, please contact [email protected]. Florida Fishery-Wide Reproductive Indices of Stone Crab, Menippe mercenaria, and Their Application to Stock Assessment and Management by Claire Elizabeth Crowley A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy with a concentration in Marine Resource Assessment College of Marine Science University of South Florida Major Professor: Kendra L Daly, Ph.D. Ryan L. Gandy, Ph.D. David Chagaris, Ph.D. Cameron Ainsworth, Ph.D. Christopher Stallings, Ph.D. Date of Approval: April 17th, 2017 Keywords: stone crab, size at maturity, histology, fecundity, spawning potential ratios Copyright © 2017, Claire E. Crowley DEDICATION I dedicate this work to my parents, Linda and Paul Crowley. Thank you for fostering my love of animals, Mother Nature, and learning. You both taught me, in your own ways, that I could truly accomplish anything that my heart desired. In sixth grade when I, your only child, told you I would leave Montana to go to college, you did everything you could to ensure that my dreams would come true. From saving money, working at school events to help my marching band’s travel expenses, spending many late nights helping me with procrastinated projects, and attending every tennis match, even if that meant sitting in the cold and rain for 12 hours at a time. Despite teenage angst, differing opinions, and a few doors slamming (literally and figuratively), there is not a time in my life that I ever questioned your unconditional love and support. Every mountain climbed, degree earned, and challenge overcome, were made possible because of you. Love you forever. Love, Mouse / Button ACKNOWLEDGEMENTS I would like to acknowledge the sources of funding that made this research possible: Florida stone crab commercial licensing and trap tag fees provided by the State of Florida, to the FWRI stone crab fishery independent monitoring program, and the Bill Hogarth Endowed Fellowship. A special thank you to Dr. Kendra Daly for advising me through both my Master’s and Doctoral degrees. Her guidance, support, compassion, and encouragement were incredibly meaningful to me throughout this process. I would also like to thank Dr. Ryan Gandy, my supervisor at the Florida Fish and Wildlife Research Institute. Without his leadership, understanding, friendship, and patience, the completion of this degree, while working full time, would not have been possible. I would also like to thank my committee member, Dr. David Chagaris for his guidance on the fifth chapter of this dissertation. I greatly appreciate his knowledge, assistance, support, and many phone calls. Thank you to my committee members, Dr. Christopher Stallings and Dr. Cameron Ainsworth for your support, editorial comments, and challenging me to think outside the box. I would like to give thanks and acknowledgement to Dr. Erin Hoerl Leone and Dr. Colin Shea for their expertise in statistics and ecological modeling, their guidance, and teaching skills were invaluable. I give my most heartfelt thanks to my friends and colleagues at the Florida Fish and Wildlife Research Institute. Not only for the hundreds of hours spent by my side collecting, dissecting, and processing histological samples, but for the support, understanding, and, most importantly, the endless laughter that brightened so many of my days. I can’t imagine having a more amazing, kind, and crazy work family. Finally, I would like to thank my parents and the friends that have been by my side since kindergarten, those from college, and those that have come into my life more recently. You are more than just friends, you are my family too. Without you, the mountains looked impossible to climb, but your words of wisdom, encouragement, and unconditional love gave me strength when I needed it the most. I am forever grateful that our paths intersected. TABLE OF CONTENTS List of Tables ............................................................................................................................................... iv List of Figures ............................................................................................................................................ v Abstract ....................................................................................................................................................... vii Chapter One. Introduction ............................................................................................................................ 1 Chapter Two. A Florida Fishery–wide Assessment of Maturity in the Stone Crab, Menippe mercenaria: Female Development ................................................................................................................ 6 Introduction ..................................................................................................................................... 6 Methods ........................................................................................................................................... 9 Field Sampling and historical data ...................................................................................... 9 Ovary sampling and preservation ........................................................................ 10 Physiological Maturity and Ovarian Staging .................................................................... 11 Oogonia ................................................................................................................ 11 Early primary growth (EPG) ................................................................................ 11 Late primary growth (LPG) ................................................................................. 12 Early secondary growth (ESG) ............................................................................ 12 Late secondary growth (LSG) .............................................................................. 12 Spent ................................................................................................................ 12 Atresia ................................................................................................................ 13 Behavioral Maturity .......................................................................................................... 13 Morphological Maturity .................................................................................................... 13 Functional Maturity .......................................................................................................... 14 Statistical Analysis ............................................................................................................ 14 Results .......................................................................................................................................... 15 Description of Catch ......................................................................................................... 15 Physiological Maturity ...................................................................................................... 15 Behavioral Maturity .......................................................................................................... 16 Morphological Maturity .................................................................................................... 16 Functional Maturity .......................................................................................................... 17 Decision-Tree Analyses .................................................................................................... 17 Discussion ...................................................................................................................................... 18 Tables .......................................................................................................................................... 25 Figures .......................................................................................................................................... 26 Chapter Three. A Florida Fishery-Wide Assessment of Maturity in the Stone Crab, Menippe mercenaria: Male Development .................................................................................................. 35 Introduction .................................................................................................................................... 35 Methods ........................................................................................................................................