Catalog # Aliquot Size

U214-911-05 3 x 5 nmol U214-911-20 3 x 20 nmol U214-911-50 3 x 50 nmol

UBE2D2 (UBC4) siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # U214-911

Lot # Z2109-19

Specificity Formulation UBE2D2 (UBC4) siRNAs are designed to specifically knock- The siRNAs are supplied as a lyophilized powder and down human UBE2D2 (UBC4) expression. shipped at room temperature.

Product Description Reconstitution Protocol UBE2D2 (UBC4) siRNA is a pool of three individual Briefly centrifuge the tubes (maximum RCF 4,000g) to synthetic siRNA duplexes designed to knock-down collect lyophilized siRNA at the bottom of the tube. human UBE2D2 (UBC4) mRNA expression. Each siRNA is Resuspend the siRNA in 50 µl of DEPC-treated water 19-25 bases in length. The accession number is (supplied by researcher), which results in a 1x stock NM_001093036. solution (10 µM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot Gene Aliases 1x stock solutions for storage. UBC4; PUBC1; UBC4/5; UBCH5B; E2(17)KB2 Related Products Storage and Stability The lyophilized powder is stable for at least 4 weeks at Product Name Catalog Number room temperature. It is recommended that the UBE2A U210-30H lyophilized and resuspended siRNAs are stored at or UBE2B Protein U211-30H below -20oC. After resuspension, siRNA stock solutions ≥2 UBE2C Protein U212-30H µM can undergo up to 50 freeze-thaw cycles without UBE2D1 (UBCH5A) U213-30H significant degradation. For long-term storage, it is Protein recommended that the siRNA is stored at -70oC. For most UBE2D2 (UBC4) Protein U214-30H favorable performance, avoid repeated handling and UBE2D3 (UBCH5C) U215-30H multiple freeze/thaw cycles. Protein

Scientific Background UBE2D2(UBC4) Protein or -conjugating enzyme E2D 2 is a member of the E2 ubiquitin-conjugating UBE2D2 (UBC4) siRNA Set I siRNA duplexes targeted against three exon regions enzyme family which functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 Catalog # U214-911 ubiquitin-protein ligase. UBE2D2 specifically ubiquitinates Lot # Z2109-19 E6AP and in vivo inhibition of UBE2D2 can lead to Packaging Specifications 2.5 nmol/tube for 3 x 5nmol inhibition of E6-stimulated p53 degradation (1). UBE2D2 could conjugate ubiquitin to target in an E6AP- Format Lyophilized powder Stability 1yr at -70oC from date of shipment dependent manner (2). Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized References and resuspended siRNAs are stored at 1. Rolfe, M.et.al: Reconstitution of p53-ubiquitylation or below -20oC. After resuspension, reactions from purified components: the role of siRNA stock solutions ≥2 µ M can human ubiquitin-conjugating enzyme UBC4 and E6- undergo up to 50 freeze-thaw cycles without significant degradation. For associated protein (E6AP). Proc. Nat. Acad. Sci. 92: long-term storage, it is recommended 3264-3268, 1995. that the siRNA is stored at -70oC. For 2. Jensen, J. P.et.al: Identification of a family of closely most favorable performance, avoid repeated handling and multiple related human ubiquitin conjugating enzymes. J. Biol. freeze/thaw cycles. Chem. 270: 30408-30414, 1995.

To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com

FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.