ACSL5 (NM_016234) Human cDNA ORF Clone

Cat. No. Description Myc -DDK -tagged ORF clone of Homo sapiens acyl -CoA synthetase long - RC201076 chain family member 5 (ACSL5), transcript variant 1 as transfection-ready DNA, 10µg

TrueORF Data

Vector: pCMV6-Entry Tag : C-terminal Myc- Western validation DDK with an anti-DDK Sequence ORF Nucleotide ORF Size: 2220 bp antibody * Data: Sequence and Predicted Protein L: Control HEK293 Protein Sequence, see MW: 82.1 kDa lysate below R: Over-expression Restriction SgfI-MluI Cloning Scheme for this gene, see lysate Sites: below OTI This clone was engineered to express the complete Annotation: ORF with an expression tag. OTI The molecular sequence of this clone aligns with Disclaimer: the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. Product The ORF clone is ion-exchange column purified, Components: transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. Protein Transmembrane Families: Protein Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway, Pathways: Adipocytokine signaling pathway

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq : NM_016234.3, RefSeq Size: 3372 RefSeq ORF: 2220 NP_057318 Synonyms : ACS2; ACS5; FACL5 LocusID: 51703 Cytogenetic: 10q25.1 Domains: AMP-binding Summary: The protein encoded by this gene is an isozyme of the long-chain fatty-acid- coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.

> ORF Nucleotide sequence

RC201076 representing NM_016234 Red =Cloning site Blue=ORF Green =Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCC GCGATCGC C

ATGGACGCTCTGAAGCCACCCTGTCTCTGGAGGAACCACGAGCGAGGGAAGAAGGACAGGGACTCGTGTG GCAGGAAGAACTCAGAGCCGGGAAGCCCCCATTCACTAGAAGCACTGAGAGATGCGGCCCCCTCGCAGGG TCTGAATTTCCTGCTGCTGTTCACAAAGATGCTTTTTATCTTTAACTTTTTGTTTTCCCCACTTCCGACC CCGGCGTTGATCTGCATCCTGACATTTGGAGCTGCCATCTTCTTGTGGCTGATCACCAGACCTCAACCCG TCTTACCTCTTCTTGACCTGAACAATCAGTCTGTGGGAATTGAGGGAGGAGCACGGAAGGGGGTTTCCCA GAAGAACAATGACCTAACAAGTTGCTGCTTCTCAGATGCCAAGACTATGTATGAGGTTTTCCAAAGAGGA CTCGCTGTGTCTGACAATGGGCCCTGCTTGGGATATAGAAAACCAAACCAGCCCTACAGATGGCTATCTT ACAAACAGGTGTCTGATAGAGCAGAGTACCTGGGTTCCTGTCTCTTGCATAAAGGTTATAAATCATCACC AGACCAGTTTGTCGGCATCTTTGCTCAGAATAGGCCAGAGTGGATCATCTCCGAATTGGCTTGTTACACG TACTCTATGGTAGCTGTACCTCTGTATGACACCTTGGGACCAGAAGCCATCGTACATATTGTCAACAAGG CTGATATCGCCATGGTGATCTGTGACACACCCCAAAAGGCATTGGTGCTGATAGGGAATGTAGAGAAAGG CTTCACCCCGAGCCTGAAGGTGATCATCCTTATGGACCCCTTTGATGATGACCTGAAGCAAAGAGGGGAG AAGAGTGGAATTGAGATCTTATCCCTATATGATGCTGAGAACCTAGGCAAAGAGCACTTCAGAAAACCTG TGCCTCCTAGCCCAGAAGACCTGAGCGTCATCTGCTTCACCAGTGGGACCACAGGTGACCCCAAAGGAGC CATGATAACCCATCAAAATATTGTTTCAAATGCTGCTGCCTTTCTCAAATGTGTGGAGCATGCTTATGAG CCCACTCCTGATGATGTGGCCATATCCTACCTCCCTCTGGCTCATATGTTTGAGAGGATTGTACAGGCTG TTGTGTACAGCTGTGGAGCCAGAGTTGGATTCTTCCAAGGGGATATTCGGTTGCTGGCTGACGACATGAA GACTTTGAAGCCCACATTGTTTCCCGCGGTGCCTCGACTCCTTAACAGGATCTACGATAAGGTACAAAAT GAGGCCAAGACACCCTTGAAGAAGTTCTTGTTGAAGCTGGCTGTTTCCAGTAAATTCAAAGAGCTTCAAA AGGGTATCATCAGGCATGATAGTTTCTGGGACAAGCTCATCTTTGCAAAGATCCAGGACAGCCTGGGCGG AAGGGTTCGTGTAATTGTCACTGGAGCTGCCCCCATGTCCACTTCAGTCATGACATTCTTCCGGGCAGCA ATGGGATGTCAGGTGTATGAAGCTTATGGTCAAACAGAATGCACAGGTGGCTGTACATTTACATTACCTG GGGACTGGACATCAGGTCACGTTGGGGTGCCCCTGGCTTGCAATTACGTGAAGCTGGAAGATGTGGCTGA CATGAACTACTTTACAGTGAATAATGAAGGAGAGGTCTGCATCAAGGGTACAAACGTGTTCAAAGGATAC CTGAAGGACCCTGAGAAGACACAGGAAGCCCTGGACAGTGATGGCTGGCTTCACACAGGAGACATTGGTC GCTGGCTCCCGAATGGAACTCTGAAGATCATCGACCGTAAAAAGAACATTTTCAAGCTGGCCCAAGGAGA ATACATTGCACCAGAGAAGATAGAAAATATCTACAACAGGAGTCAACCAGTGTTACAAATTTTTGTACAC GGGGAGAGCTTACGGTCATCCTTAGTAGGAGTGGTGGTTCCTGACACAGATGTACTTCCCTCATTTGCAG CCAAGCTTGGGGTGAAGGGCTCCTTTGAGGAACTGTGCCAAAACCAAGTTGTAAGGGAAGCCATTTTAGA AGACTTGCAGAAAATTGGGAAAGAAAGTGGCCTTAAAACTTTTGAACAGGTCAAAGCCATTTTTCTTCAT CCAGAGCCATTTTCCATTGAAAATGGGCTCTTGACACCAACATTGAAAGCAAAGCGAGGAGAGCTTTCCA AATACTTTCGGACCCAAATTGACAGCCTGTATGAGCACATCCAGGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA

> Protein sequence

RC201076 representing NM_016234 Red=Cloning site Green=Tags(s)

MDALKPPCLWRNHERGKKDRDSCGRKNSEPGSPHSLEALRDAAPSQGLNFLLLFTKMLFIFNFLFSPLPT PALICILTFGAAIFLWLITRPQPVLPLLDLNNQSVGIEGGARKGVSQKNNDLTSCCFSDAKTMYEVFQRG LAVSDNGPCLGYRKPNQPYRWLSYKQVSDRAEYLGSCLLHKGYKSSPDQFVGIFAQNRPEWIISELACYT YSMVAVPLYDTLGPEAIVHIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQRGE KSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPKGAMITHQNIVSNAAAFLKCVEHAYE PTPDDVAISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLADDMKTLKPTLFPAVPRLLNRIYDKVQN EAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAA MGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGY LKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVH GESLRSSLVGVVVPDTDVLPSFAAKLGVKGSFEELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLH PEPFSIENGLLTPTLKAKRGELSKYFRTQIDSLYEHIQD

TRTRRLEQKLISEEDLAANDILDYKDDDDKV

> Cloning scheme