Curriculum Vitae PERSONAL DETAILS Miao-Hsia Lin (Research Scholar) E-mail：[email protected] TEL: +886-958-806-350 Mailing Address: No. 303, Zhongbei 3rd St., Linkou Dist., New Taipei City, Taiwan
POSITIONS HELD
2018.08 – present Research Scholar, Institute of Chemistry, Academia Sinica, Taipei, Taiwan 2016.03 – 2018.02 Postdoctoral fellow, Biomolecular Mass Spectrometry and Proteomics, Utrecht University, Utrecht, Netherlands
2014.02 – 2016.02 Postdoctoral fellow, Institute of Chemistry, Academia Sinica, Taipei, Taiwan
2012.04 – 2014.01 JSPS Postdoctoral fellow, Department of Molecular & Cellular Bioanalysis, Graduate School of Pharmaceutical Science, Kyoto University, Kyoto, Japan
2010.02 – 2012.03 Postdoctoral fellow, Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan
EDUCATION
2010.01 Ph.D Institute of Biochemical Science, National Taiwan University, Taiwan 2005. 06 M.S. Institute of Biochemical Science, National Taiwan University, Taiwan 2003. 06 B.S. Department of Life Sciences, Tzu Chi University, Taiwan
HONORS AND AWARDS
2000.10 Presidential Award, Tzu Chi University
2001.03 Presidential Award, Tzu Chi University 2001.10 Presidential Award, Tzu Chi University
2002.03 Presidential Award, Tzu Chi University 2002.10 Presidential Award, Tzu Chi University 2003.03 Presidential Award, Tzu Chi University
2008.07 Presentation Award, The 5th conference of Glycoscience and Chemical Biology 2009.09 Young investigator Award, Human Proteomics (HUPO) 8th Annual World Congress 2009.12 Significant Research Achievements, Academia Sinica 2010.07 Presentation award, 2010 International Symposium of Glycosciences 2011.09 Scholarship for postdoctoral fellowship, Japan Society for the Promotion of Science 2013.07 Young Scientist Award, 4th Asia Oceania Mass Spectrometry Conference and 10th Taiwan Society for Mass Spectrometry Annual Conference
RESEARCH EXPERTISE Mass Spectrometry Experience - Experienced in the running and maintenance of Thermo and AB Sciex MS instrumentation. - Extensive experience in the use of modern mass analysers from Waters (Waters Synapt HDMS, G2), AB Sciex (Triple Quad) and Thermo (Q- Exactive, Fusion. Lumos). - Skilled in the sample preparation and MS-based protein post-translational modification analysis to solve a variety of biological problems. - Experienced in the application of CID, HCD, ETD and EThcD fragmentation methods to identify post-translation modifications at the peptide level. - LC-MS experience using the Waters nanoAquity LC system, Agilent and Dionex systems for peptide identification, protein purification, polysaccharides separation.
Biochemical Experience - Expertise in the use of biochemical techniques including cell culture, analytical gel filtration, gel electrophoresis, western blotting and chromatography techniques (HPLC, UPLC). - Extensive experience in molecular biology, PCR, gene cloning, recombinant protein expression and purification of cytoplasmic proteins, periplasmic protein complexes, and membrane proteins.
LECTURES
1. HUPO 12th Annual World Congress, Yokohama, Japan. (2013). Title: Enhanced phosphopeptide identification in Escherichia coli by stepwise hydroxy acid-modified metal oxide chromatography. 2. Young Scientist Forum, 4th Asia Oceania Mass Spectrometry Conference and 10th Taiwan Society for Mass Spectrometry Annual Conference, Taipei, Taiwan. (2013). Title: Enhanced phosphopeptide identification in Escherichia coli by stepwise hydroxy acid-modified metal oxide chromatography with elevated sample loading capacity. 3. International Symposium of Glycosciences, Taipei, Taiwan. (2010). Title: A Novel Exopolysaccharide from the Biofilm of Thermus aquaticus YT- 1 Induces the Immune Response through Toll-like Receptor 2. 4. The 5th conference of Glycoscience and Chemical Biology (2008). Topic: Structure characterization and immuno-modulatory effect of a noval exopolysaccharide from the biofilm of Thermus Aquaticus YT-1 5. Class : Advanced biotechnology laboratory for herbal medicines, School of Pharmacy, Taipei Medical University (2011). Topic: Current approach in phosphoproteomic analysis Experimental demonstration: Phosphoprotein identification by mass spectrometry and Data analysis
REFERENCES
Prof. Dr. Albert J.R. Heck (Postdoctoral supervisor) Biomolecular Mass Spectrometry and Proteomics, Utrecht University Padualaan 8, 3584 CH, Utrecht, The Netherlands E-mail: [email protected] Tel: +31-302536797
Prof. Dr. Yasushi Ishihama (Postdoctoral supervisor) Department of Molecular & Cellular BioAnalysis, Kyoto university 46-29 Yoshida-Shimo-Adachi-cho, Sakyo-ku, Kyoto 606-8501, Japan E-mail: [email protected] Tel: +81-757534565
Prof. Dr. Yu-Ju Chen (Postdoctoral supervisor) Institute of Chemistry, Academia Sinica No. 128, Sec. 2. Academia Rd., Nankang, Taipei 115, Taiwan E-mail: [email protected] Tel: +886-227898660
Prof. Dr. Shih-Hsiung Wu (PhD supervisor) Institute of Biological Chemistry, Academia Sinica No. 128, Sec. 2. Academia Rd., Nankang, Taipei 115, Taiwan E-mail: [email protected] Tel: +886-227855696#7101 5-year Publications
1. Clement Potel, Miao-Hsia Lin, Nadine Prust, Henk W. P. van den Toorn, Albert J.R. Heck, Simone Lemeer*. “Gaining evidence of Confidence in the Elusive Histidine Phosphoproteome”. Anal Chem., (2019). 91(9): 5542–5547.
2. Naoyuki Sugiyama, Satomi Miyake, Miao-Hsia Lin, Masaki Wakabayashi, Hiroyuki Marusawa, Shin Nishiumi, Masaru Yoshida, Yasushi Ishihama*. “Comparative proteomics of Helicobacter pylori reveals geographical features rather than genomic variations”. Genes Cells., (2019). 24(2):139-150.
3. Miao-Hsia Lin, Biswanath Chatterjee, Chun-Chang Chen, Kai-Lin Peng, Mu-Sheng Wu, Mei-Chun Tseng, Yu-Ju Chen*, Che-Kun James Shen*. “DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells”. Biochim Biophys Acta Gene Regul Mech., (2018). 1861(11):1048-1061. 4. Miao-Hsia Lin, Clement Potel, Kamaleddin H.M.E. Tehrani, Albert J.R. Heck, Nathaniel I. Martin, Simone Lemeer*. “A new tool to reveal bacterial signaling mechanisms in antibiotic treatment and resistance”. Mol. Cell. Proteomics., (2018). pii: mcp.RA118.000880. 5. Sabrina Mai-Yi Fan, Chia-Feng Tsai, Chien-Mei Yen, Miao-Hsia Lin, Wei-Hung Wang, Chih-Chieh Chan, Chih-Lung Chen, Kyle K.L. Phua, Szu-Hua Pan, Maksim V. Plikus, Sung-Liang Yu, Yu-Ju Chen*, Sung-Jan Lin*. “Inducing Hair Follicle Neogenesis with Secreted Proteins Enriched in Embryonic Skin”. Biomaterials., (2018). 167:121-131. 6. Miao-Hsia Lin, Clement Potel, Albert J.R. Heck*, Simone Lemeer*. “Defeating Major Contaminants in Fe3+- Immobilized Metal Ion Affinity Chromatography (IMAC) Phosphopeptide Enrichment”. Mol. Cell. Proteomics., (2018). 17(5):1028- 1034. 7. Miao-Hsia Lin, Clement Potel, Albert J.R. Heck*, Simone Lemeer*. “Widespread Bacterial Protein Histidine Phosphorylation Revealed by Mass Spectrometry- based Proteomics”. Nat Methods., (2018). 15(3):187-190. 8. Sui-Chih Tien, Hsiao-Hui Lee, Ya-Chi Yang, Miao-Hsia Lin, Yu-Ju Chen, Zee-Fen Chang*. “The Shp2-induced Epithelial Disorganization Defect is Rreversed by HDAC6 Inhibition Independent of Cdc42” Nat. Commun., (2016).7:10420. 9. Tanujaa Suriyanarayanan, Saravanan Periasamy, Miao-Hsia Lin, Yasushi Ishihama, Sanjay Swarup*. “Flagellin FliC Phosphorylation Affects Type 2 Protease Secretion and Biofilm Dispersal in Pseudomonas aeruginosa PAO1” PLos One, (2016). 11(10):e0164155. 10. Miao-Hsia Lin, Naoyuki Sugiyama, and Yasushi Ishihama*. * “Systematic Profiling of the Bacterial Phosphoproteome Reveals Bacterium-specific features of Phosphorylation” Sci. Signal., (2015). 8, rs10. Manuscript submitted 1. Miao-Hsia Lin, Chia-Feng Tsai, Wei-Ting Lai, Pei-Yi Lin, Yu-Ju Chen*. “In-Depth Phosphoproteome Analysis by Weak- and Strong-coordination Metal Oxide Chromatography” Mol. Cell. Proteomics. (Under revision). 2. Tzu-Hua Wu, Jhih-Tian Yang, Chang-Shiann Wu, Miao-Hsia Lin, Wan-Ling Wu, Cheng-Wei Lai, Sheng-Hsuan Ku, Yu-Chun Hung, Chia-Te Chou, Chien-Sheng Wang, Yasushi Ishihama, Jiahn-Haur Liao*, Shih-Hsiung Wu*. “Phosphoproteomic Analysis Reveals a Key Role for AKAP4 in Mouse Sperm Capacitation”. J. Proteome Res. (Under revision).