Affinity Biosciences website:www.affbiotech.com order:[email protected] COX17 Antibody

Cat.#: AF0626 Concn.: 1mg/ml Mol.Wt.: 7kDa Size: 50ul,100ul,200ul Source: Rabbit Clonality: Polyclonal

Application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 *The optimal dilutions should be determined by the end user. Reactivity: Human,Mouse,Rat

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity: COX17 Antibody detects endogenous levels of total COX17.

Immunogen: A synthesized peptide derived from human COX17, corresponding to a region within N-terminal amino acids.

Uniprot: Q14061

Description: (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to . This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear encodes a which is not a structural subunit, but may be involved in the recruitment of copper to mitochondria for incorporation into the COX apoenzyme. This protein shares 92% amino acid sequence identity with mouse and rat Cox17 . This gene is no longer considered to be a candidate gene for COX deficiency. A pseudogene COX17P has been found on 13.

Storage Condition and Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM Buffer: NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

1 / 2 Affinity Biosciences website:www.affbiotech.com order:[email protected]

Western blot analysis of extracts from 293, using COX17 Antibody.

AF0626 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

AF0626 at 1/100 staining human skeletal muscle tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

AF0626 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

For Research Use Only. Not for use in diagnostic and therapeutic procedures. Not for resale without express authorization.

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