Article Title: Construction of a Genetic Linkage Map Based on RAPD, AFLP, and SSR Markers
Article title: Construction of a genetic linkage map based on RAPD, AFLP, and SSR markers for tea plant (Camellia sinensis); Journal name: Euphytica; Author names: Yali Chang, Eun Ui Oh, Min Seuk Lee, Ho Bang Kim, Doo-Gyung Moon, and Kwan Jeong Song; Corresponding author: Kwan Jeong Song, Research Institute for Subtropical Agriculture & Biotechnology, Jeju National University, Jeju 63243, Korea, and E-mail: .
Online resource 2. RNA-seq based SSR markers being polymorphic between ‘Fushun’ and ‘Kemsull’. Notes: a, SSR markers with underline were used into mapping analysis; b, the anticipated band size in silico; c, ‘f’ means ‘Fushun’, ‘k’ means ‘Kemsull’; d, illuminate why some SSR markers with admissible genotypes by JoinMap 4.0 could not apply into linkage analysis.
IDa / Forward primer (5'-3') / Reverse primer (5'-3') / SSR unit type / Band sizeb / Genotype (f-k)c / NotesdKF0007 / TTTCACCAGGACGAATGCACTA / CACAGAATGCAGTGCTATGAAA / (ACAATA)2 / 194 / aa-ab
KF0036 / TATAAACCACCTCGCGAGACTC / GGGGCTGGTGTTCTTCATAGAA / (TCA)8 / 362 / ac-bd
KF0043 / TTTGCTCTGCATTTTGCTGC / ATGCTGCTTGAGAGACCATGAA / (ATCAAA)6 / 260 / ab-cd
KF0055 / ATGTGGTTGGTTCTGACTCGAT / ACTTGCCGGGTTAAGCGTATTA / (TTC)5 / 249 / abc-cc
KF0073 / CTTGTTCCCTCCTATGCCCTTT / ATGGCGCAAAATCATCATACGG / (CCACCT)4 / 192 / ab-bcd
KF0097 / CCTCCTACACAGCATCACCATT / TAATCTCACTCCTTTCGGTGGC / (CACCGC)2 / 206 / cc-ab
KF0112 / GTAGAAATCGCCGAAATCACCG / AACTCTTTGCTCCAGCTCATCA / (ACCCGG)2 / 212 / ab-ac
KF0120 / TGGGGCGTTGAACAATCATCTA / CCTGCCTGAATCACAAAACCAG / (TTGATG)2 / 194 / ab-ab
KF0134 / TACCAGCGTGTCCTAAAACTCC / CCTAACGAAGCAAAAACGAGCA / (CTAGCC)3 / 250 / aa-bb
KF0141 / GCATGAAGAGCTCGCCTTTAAG / ACCTTCCATGGCATTCGAATCT / (AAG)7 / 208 / ab-ab
KF0147 / CCTTGGCTACAAAGAGCAGAGA / GACCTTGCTAAAGTTGGTGCAG / (CAACAG)2 / 387 / ac-ab
KF0166 / ATCAGCTCGAATCAAACCCCTT / TTTGGGTTTTGCGGGGTTAATC / (TCGCTC)2 / 189 / cd-ab / PCR amplicons were unclear and most F1 seedlings were genotype ‘abcd’.
KF0191 / GCAGAAAGAAAGGGGCGAAAAT / GCTTCATCTACTTCGTCTCCGT / (AAG)5 / 202 / abcd-ac
KF0213 / AGATGTAACTCTAGCAGTGGC / GGAAAGGCTAATCGGGATAGGG / (ACC)7 / 279 / bb-abc
KF0217 / AGCCTCATGCCCAAATCTTGTA / GCAACTGGATGATGGTGATGTG / (TTTC)9 / 399 / aa-ab / PCR amplification failure.
KF0231 / AAAACTTCCCGATTCCTCCTCC / TCAGACAATGGACGCTGATGAA / (TCT)7 / 398 / ab-aa / PCR amplification failure.
KF0234 / CACTCTCTCTCACAGAAACCCC / ACGTGTTTCTTCGCTAGTGGAT / (TACGAC)3 / 273 / aa-ab
KF0236 / TCAAAATCGAGTGCGCCCTATA / AGGGATGACCAAACAGGATTCC / (AGG)5 / 268 / abc-ac
KF0251 / CCAACCCAACCTAGTCTACCAC / AAAGTGGATGTGTCCCTCTTCC / (GGCCAT)4 / 297 / ac-bc / PCR amplification failure.
KF0265 / GTGTTCTTCAGTGATCCCGAGT / TCTGGTTTCGCATCTTGCTAGT / (GGT)6 / 373 / aa-bc
KF0269 / CACCTCCTCCTCAAAAGGTCTC / GGGGACACACATGCCTATTACT / (AACACA)3 / 398 / aa-ab / PCR amplicons were undistinguishable.
KF0271 / TGCAACAGCCTTCATACTGCTA / GCCGCATGCTGTTAAAAGCTAA / (GCAG)4 / 344 / bb-ab
KF0288 / TCATGGGTCATTCATCGTTGGT / TGGAATTCAAAGTGAGCTCCGA / (AACCCT)2 / 361 / ab-aa
KF0289 / CCCAAATCCCAAGCAGTAGAGT / TGCTAACAATGGAAGCATCCCT / (GTA)6 / 228 / ac-bc / PCR amplification failure.
KF0290 / AGAGTGGGCTTTGATGATTCGT / CCACCCCTTCGAAAATTATAACCA / (GAGAT)3 / 212 / bb-ac / PCR amplification failure.
KF0304 / CTGGAGGCTGAATTGAGGAAGT / TTCTTCACAGGACAAGCACAGT / (CGA)5 / 356 / ab-bb / PCR amplification failure.
KF0305 / AGACAAAGCTATGACCCTGGTT / CTCCAGAAAACCCATCCACAGA / (TGAGT)3 / 260 / ab-aa
KF0311 / GCGTTGACAATGCTAACTTGGT / ACCTGTCCATGTTCCCTTTTGT / (AATGAACT)2 / 214 / aa-ab / PCR amplification failure.
KF0320 / CTCTCCCTGGGCTTGAATGAAT / GAACTCACAGCAACAACATCCC / (TTG)7 / 303 / ac-ab / PCR amplification failure.
KF0340 / CAAAGTGGAATGCACCGATTGT / ATTGGCTTTCAATCCAGCTATGG / (TATTAC)3 / 207 / ac-bb
KF0350 / TTTTTCATCTTCCGAATGGCGC / GGCTATTTGCTGGGTTCCAAAG / (CGG)5 / 228 / aa-ab
KF0353 / AATTCCCACCACGACTCTCTTC / TGATTCCAACTCCGACAACGAT / (AGGGTT)4 / 233 / cd-ab / Most F1 seedlings were ‘abcd’ genotypes.
KF0365 / GTGACTCCGTAGATGGTGATCC / CTTGGCCCTCTACATCCCTTTT / (TGATGT)8 / 420 / ab-cc
KF0382 / AGCTCAAGGATTGGTTTCCGAT / CACCCAGAGAGTGCAGTAAAGT / (TTTCT)5 / 388 / aa-ab
KF0387 / GGTTTCCCCCTTGTATGGCTTA / AGGCCTAAACTTCAAACCCTGT / (CTGATG)2 / 398 / ac-ab / PCR amplification failure.
KF0391 / TCTACCTCCTTAGAACCCTGCA / GTCGTCGTAGTTCTCGAAGTCA / (CCA)5 / 319 / bb-ab / PCR amplification failure.
KF0403 / AAGGTGGCCATCATGTTCATCT / GCTGAGACTCTGTCGCAATTTC / (CAA)7 / 187 / abcd-abcd
KF0411 / TTACCATCCTCCTCCACCTGAT / CACTCAAGCAAACAGAGCAGTC / (TCC)10 / 380 / aa-bb
KF0413 / GCATCTACTGACATTGCACGTC / TGCACATTTCTTGAGATGTTTACT / (GTTGATT)2 / 191 / abcd-cde
KF0416 / AGCAAAGAGTCAGCTGGATCAA / AGAGAGACAGAGAGATGGGCAA / (AATCCT)4 / 261 / bb-ab
KF0434 / GGAAATTCTGTTGTTGACCGCA / ACCAGCTGAGGATGTTGAGAAG / (CCCTAA)4 / 344 / bc-aa
KF0453 / TTCCCACCACCATACTCTTTCG / CTGAGGAGACCCAAACTGTAGG / (TGCCCT)4 / 187 / ab-bc
KF0468 / CTAAATCGGCTTCGTCGTTGTC / TCTTACTGTTCTTCGGTTCCGG / (ATCAGA)4 / 315 / bb-ab
KF0502 / TGAGGTTCAATCGGCTACACAA / GGAGGTTCCCCAAATGGAGAAT / (GAGTGG)4 / 231 / ab-bcd
KF0503 / AACAATCACTGAGGTCGATGCT / AACCCATCCCAGAAACAGATCC / (GGT)9 / 365 / ab-ac / PCR amplification failure.
KF0512 / TGCACCTCCAAACCCAATTACT / CCTCCCCAAGCTCATACTTGTT / (GGAGGT)2 / 436 / bb-ab
KF0515 / ATTCCCCATGTTTGCATACCCT / AGCTCTACATGTCACCATCGTC / (GTC)5 / 305 / ac-bd / Most F1 seedlings were ‘abcd’ genotypes.
KF0536 / ACAGTCAAGCCTCCATTCCTTT / GTTAGGGGGTTTGGAATCGCTA / (ATC)11 / 249 / ab-ac
KF0542 / TCCCAAATGCTTTCTCTCTCCT / TCATTGGCTTGGATGGTCTCAA / (AACAAT)2 / 430 / abc-ab
KF0545 / CTCTGTTCTTCGATCCACACCA / CGAAGAACCAAGAAGCTCGTTC / (TCTTCG)5 / 203 / ac-ab
KF0551 / GTGAGGGAAGTTGACGTTAGGT / GCGGCATGTCAAAGTATTTCGT / (GAA)8 / 361 / ac-bd / PCR amplicons were weak and most F1 seedlings were ‘abcd’ genotypes.
KF0587 / AGGACATTGCTTCCTACTGGTG / ATATGGGTGTCAAGCAAGCAGA / (TCGTCA)3 / 189 / bb-aa
KF0593 / AAATTCATCGTCGTTTTGCGGT / CTGAGAGAGGTGGAGAGACAGA / (TCT)5 / 222 / ac-bd / most F1 seedlings were ‘abcd’ genotypes.
KF0595 / TTGTTCCTCTTCAACCCCAACA / AACTGAACTGGAGATTCGGCAT / (AATCCG)3 / 179 / ab-bb
KF0606 / GCAAAAATATCACAAGAGAACTGA / TGATGAGTACTGCCGTTGTTCA / (CTCATC)6 / 164 / aa-ab / PCR amplification failure.
KF0607 / TGAACAACGGCAGTACTCATCA / CATAAGGCGGCCTTTTGATTGG / (TCC)7 / 166 / aa-ab
KF0610 / CTCCGATAGCTTCCTTTCCCTC / TCAGATTTGGGTGTGAATCGGT / (CTC)7 / 255 / ab-abc
KF0635 / CGCACCTCAAACAGCATTCATT / GGTTGAGCAGGTTTAGGATGGA / (GCCGCCACT)2 / 305 / ab-cd / most F1 seedlings were ‘abcd’ genotypes.
KF0638 / CCCTCCCTTCTCTTCTTTTCCA / CATCGATTGCATTGCAGCAGAA / (GGCTGT)3 / 129 / cd-ab / PCR amplification failure.
KF0643 / CCATTGTTGTCAGCAGCATCAA / TTCAAGCGGCAAAGGCAATTAA / (AACCCT)5 / 338 / ab-cc
KF0657 / ACCTTCACATGCTGGGAATCAT / GGGGAGATTGTGTGAACCTCAT / (CAG)6 / 198 / bc-ab / PCR amplification failure.
KF0660 / TTGGCGTAATTTTTGTCGCAGT / TCGTCTTCCTCTTAAGTCGCAC / (CACAAC)2 / 266 / bb-aa
KF0664 / GAAGTTACCTGAGTCGTCGAGG / GGTTCAACCCTCTTAGCTTCCA / (TGACTGGGT)2 / 300 / aa-ab / PCR amplicons were weak and unclear.
KF0665 / AACGAAACCAAACAGTGTTGGG / CTCACCGCCTAATTCCTCCTAC / (GAA)6 / 234 / aa-bb
KF0684 / GTGTGATCCGGAGACAGAAACT / TCCGAAACACACTCTTCTCACG / (AAC)7 / 174 / aa-ab / PCR amplification failure.
KF0685 / ATCCTGCTGTGTGTGTCAGATT / GTGCTTCGTCTTGTTGCTTTCT / (AGGCGG)3 / 224 / ab-ab
KF0696 / TGGTACACCAATCCAGGAACAG / AACATGTTACACTGCTGGGCTA / (CTG)5 / 267 / ab-aa
KF0734 / CTGTACCAGTGCCTAATCAGGG / ACTGAACATTTGAGGTGCATGC / (CCT)7 / 194 / aa-ab / PCR amplicons were weak and unclear.
KF0768 / AGAGTGACTTGGCTAATGGTGG / GACGTAGGCAGCGAATTTCTTC / (CAA)9 / 358 / abcd-ce
KF0783 / ATATACCACTAGCCGAACTCGC / AGATGTCGTGGAGTTGCAGAAT / (ACCACT)2 / 150 / abcd-ab
KF0784 / GAGAGGAGCACAACGAAGGTAA / CCAAGACCCCTTTCCAATAGCT / (ATTGTC)3 / 132 / aa-ab / PCR amplicons were weak and unclear.
KF0799 / TCAAGTTTTGCCTGCGAATGAG / ATAATGGGGAGAAGGGGGAGAA / (TTCCCA)5 / 442 / ab-bb
KF0807 / CCCTAGCAGCTGTCTCAAAGAA / GAAGATTTTGCGGTGAGAGACG / (ACTCCG)5 / 254 / abc-bcd
KF0810 / GCAACACGCTTAGGGTTTGATT / AAGTTGAGTAGAGGGCTTCTGC / (AGAGA)4 / 257 / aa-ab